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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5397R-3

Symbol CG5397 Full Name CG5397

CG No CG5397 Old CG No CG5397

Synonyms CT16527, CG5397

Accession No (Link to NCBI) NM_134747.2

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965 [ PubMed ID = 25647511 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTGGTGATCTTGGCCAGCTGC

Primer Seq. 3'

aatctagaggtaccCGATACTGAGGAGCCACCACG

Predicted Fragment Size

IR fragment full Seq

0001 GTGGTGATCT TGGCCAGCTG CAAGTCTGGC GGGGATGCGC ACGTTCGCGT GAAGCGGATA 
0061 GTGGGTGGCA AGCAGTCGAA GGCGCCGCCC GTCGATGATC CCGTGATCTT CGCGAGACTC 
0121 TTTGATCGCG ATGCGCGAGT GGNAGGGATT CCGAAATCCC AGCACGGGCA TTTACTCGTT 
0181 TCTGGGAATG CACTATGCGN AGCCACCTGT TGGTCCACTG AGATACTCCA GACCGGTGTA 
0241 CAAAAGGCTT GCTGGTGACT TCAATGCCAC CAAACATGGG CCCACCTTGT ATACAGCCGC 
0301 ATCCGCAGTT TCCCCAAAGG ATTATCGGCG ATGAGGATTG CCTGCTGCTG AACGTCTACA 
0361 CCCCGCAAAT GCCGGATGAG ACCACAGGGC TGCCAGTGTT CGTTTGGATT CACCCGGGTG 
0421 GCTATCGATA TGGATCGGCG GCCCAGTACG ATGCCACNCC tATGgcNCAA AGGGGCGCAA 
0481 TCGTGGTGGC TCCTCAGTAT CG

in silico PCR Fragment

0001 GTGGTGATCT TGGCCAGCTG CAAGTCTGGC GGGGATGCGC ACGTTCGCGT GAAGCGGATA 
0061 GTGGGTGGCA AGCAGTCGAA GGCGCCGCCC GTCGATGATC CCGTGATCTT CGCGAGACTC 
0121 TTTGATCGCG ATGCGCGAGT GG-AGGGATT CCGGAATCCC AGCACGGGCA TCTACTCCTT 
0181 TCTGGGAATG CACTACGCGG AGCCACCTGT TGGTCCACTG AGATACTCCA GACCGGTGTA 
0241 CAAAAGGCTG GCTGGTGACT TCAATGCCAC CAAACAT-GG CCCACCTTGT ATACAGCCGC 
0301 ATCCGCAGTT TCCTCAGAGG ATTATCGGCG ATGAGGATTG CCTGCTGCTG AACGTCTACA 
0361 CCCCGCAAAT GCCGGATGAG ACCACAGGGC TGCCAGTGTT CGTTTGGATT CACCCGGGTG 
0421 GCTATCGATA TGGATCGGCG GCCCAGTACG ATGCCACGCC TATGGCCCAA AGGGGCGCAA 
0481 TCGTGGTGGC TCCTCAGTAT CG

Assemble Data


5397R-3.IR_full       1   GTGGTGATCTTGGCCAGCTGCAAGTCTGGCGGGGATGCGCACGTTCGCGTGAAGCGGATA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5397R-3.in silico     1   GTGGTGATCTTGGCCAGCTGCAAGTCTGGCGGGGATGCGCACGTTCGCGTGAAGCGGATA 60


5397R-3.IR_full       61  GTGGGTGGCAAGCAGTCGAAGGCGCCGCCCGTCGATGATCCCGTGATCTTCGCGAGACTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5397R-3.in silico     61  GTGGGTGGCAAGCAGTCGAAGGCGCCGCCCGTCGATGATCCCGTGATCTTCGCGAGACTC 120


5397R-3.IR_full       121 TTTGATCGCGATGCGCGAGTGGNAGGGATTCCGAAATCCCAGCACGGGCATTTACTCGTT 180
                          |||||||||||||||||||||| |||||||||| ||||||||||||||||| ||||| ||
5397R-3.in silico     121 TTTGATCGCGATGCGCGAGTGG-AGGGATTCCGGAATCCCAGCACGGGCATCTACTCCTT 180


5397R-3.IR_full       181 TCTGGGAATGCACTATGCGNAGCCACCTGTTGGTCCACTGAGATACTCCAGACCGGTGTA 240
                          ||||||||||||||| ||| ||||||||||||||||||||||||||||||||||||||||
5397R-3.in silico     181 TCTGGGAATGCACTACGCGGAGCCACCTGTTGGTCCACTGAGATACTCCAGACCGGTGTA 240


5397R-3.IR_full       241 CAAAAGGCTTGCTGGTGACTTCAATGCCACCAAACATGGGCCCACCTTGTATACAGCCGC 300
                          ||||||||| ||||||||||||||||||||||||||| ||||||||||||||||||||||
5397R-3.in silico     241 CAAAAGGCTGGCTGGTGACTTCAATGCCACCAAACAT-GGCCCACCTTGTATACAGCCGC 300


5397R-3.IR_full       301 ATCCGCAGTTTCCCCAAAGGATTATCGGCGATGAGGATTGCCTGCTGCTGAACGTCTACA 360
                          ||||||||||||| || |||||||||||||||||||||||||||||||||||||||||||
5397R-3.in silico     301 ATCCGCAGTTTCCTCAGAGGATTATCGGCGATGAGGATTGCCTGCTGCTGAACGTCTACA 360


5397R-3.IR_full       361 CCCCGCAAATGCCGGATGAGACCACAGGGCTGCCAGTGTTCGTTTGGATTCACCCGGGTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5397R-3.in silico     361 CCCCGCAAATGCCGGATGAGACCACAGGGCTGCCAGTGTTCGTTTGGATTCACCCGGGTG 420


5397R-3.IR_full       421 GCTATCGATATGGATCGGCGGCCCAGTACGATGCCACNCCTATGGCNCAAAGGGGCGCAA 480
                          ||||||||||||||||||||||||||||||||||||| |||||||| |||||||||||||
5397R-3.in silico     421 GCTATCGATATGGATCGGCGGCCCAGTACGATGCCACGCCTATGGCCCAAAGGGGCGCAA 480


5397R-3.IR_full       481 TCGTGGTGGCTCCTCAGTATCG 502
                          ||||||||||||||||||||||
5397R-3.in silico     481 TCGTGGTGGCTCCTCAGTATCG 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	6	6	NM_134747.2	CG5397-RA (CG5397), mRNA
0	0	3	2	6	NM_169775.1	CG7467-RA, transcript variant A (osa), mRNA
0	0	3	2	6	NM_079668.2	CG7467-RB, transcript variant B (osa), mRNA
0	0	3	2	4	NM_206506.1	CG7467-RC, transcript variant C (osa), mRNA
0	0	3	2	2	NM_132058.2	CG5966-RA (CG5966), mRNA
0	0	2	2	3	NM_079034.2	CG8425-RA (Jhe), mRNA
0	0	2	2	2	NM_078723.2	CG3022-RA, transcript variant A (GABA-B-R3), mRNA
0	0	2	2	2	NM_164387.1	CG3022-RB, transcript variant B (GABA-B-R3), mRNA
0	0	2	2	2	NM_134793.2	CG7291-RA (NPC2), mRNA
0	0	1	4	2	NM_132650.1	CG10617-RA (CG10617), mRNA
0	0	1	2	5	NM_139743.2	CG10483-RA (CG10483), mRNA
0	0	1	2	4	NM_135753.2	CG9934-RA (CG9934), mRNA
0	0	1	2	3	NM_139764.2	CG13295-RA (CG13295), mRNA
0	0	1	2	3	NM_139623.2	CG1309-RA (CG1309), mRNA
0	0	1	1	2	NM_206438.1	CG17603-RB, transcript variant B (Taf1), mRNA
0	0	1	1	2	NM_206437.1	CG17603-RC, transcript variant C (Taf1), mRNA
0	0	1	1	2	NM_057608.4	CG17603-RA, transcript variant A (Taf1), mRNA
0	0	1	1	1	NM_134613.2	CG1644-RA (Cyp6t1), mRNA
0	0	0	6	5	NM_166881.1	CG32813-RE, transcript variant E (CG32813), mRNA
0	0	0	5	8	NM_001042903.1	CG7527-RB, transcript variant B (CadN2), mRNA
0	0	0	5	8	NM_136011.2	CG7527-RA, transcript variant A (CadN2), mRNA
0	0	0	5	2	NM_133070.1	CG15046-RA (CG15046), mRNA
0	0	0	5	2	NM_136347.1	CG14471-RB, transcript variant B (CG14471), mRNA
0	0	0	4	6	NM_132430.2	CG11207-RA (feo), mRNA
0	0	0	4	5	NM_167875.1	CG9181-RD, transcript variant D (Ptp61F), mRNA
0	0	0	4	5	NM_057339.3	CG9181-RA, transcript variant A (Ptp61F), mRNA
0	0	0	4	5	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0	0	0	4	5	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA
0	0	0	4	3	NM_136702.2	CG18445-RA (CG18445), mRNA
0	0	0	4	3	NM_078676.3	CG6223-RA (betaCop), mRNA

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