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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5374R-2 |
| Symbol |
T-cp1 |
Full Name |
Tcp1-like |
| CG No |
CG5374 |
Old CG No |
CG5374 |
| Synonyms |
T-cpl, gh05123, BEST:GH05123, Tcp-1, CG5374, T-cp1 |
| Accession No (Link to NCBI) |
NM_079726.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Kim AR, Choi KW.<br> TRiC/CCT chaperonins are essential for organ growth by interacting with insulin/TOR signaling in Drosophila.<br> Oncogene (2019) 38(24) 4739-4754
[
PubMed ID = 30792539
]
[
RRC reference
]
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.<br> Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.<br> PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Jia D, Soylemez M, Calvin G, Bornmann R, Bryant J, Hanna C, Huang YC, Deng WM.<br> A large-scale in vivo RNAi screen to identify genes involved in Notch-mediated follicle cell differentiation and cell cycle switches.<br> Sci Rep (2015) 5 12328
[
PubMed ID = 26205122
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCATCGGTGCGCACACAGAACG |
| Primer Seq. 3' |
aatctagaggtaccGATTTTCACCGACTGGGCAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CATCGGTGCG CACACAGAAC GTTATGGCCG CTCTGTCCAT ATCGAACATC GTGAAAAGCT
0061 CCTTGGGACC CGTGGGTCTG AGACAAGATG CTGGTGGACG ACATCGGCGA TGTCACAGTG
0121 ACCAACGATG GAGCCACCAT TCTGCGACTG CTGGAGGTGG AGCACCCGGC AGCCAAGGTC
0181 CTGGTTGAGC TGGCTCAGCT GCAGGACGAG GAGGTCGGCG ATGGCACTAC CTCGGTGGTT
0241 ATCCTGGCCG CTGAGCTGCT GAAAAACGCC GACGAGCTGG TCAAGCAGAA GATTCATCCT
0301 ACCTCCATAA TCTCCGGCTA CCGCATTGCC TGCAAGGAGG CCTGCAAGTA CATCTCGGAG
0361 CATCTGACCG CTCCCGTTGA TGAGCTGGGC CGCGATTCCC TGATTAACAT TGCCAAGACC
0421 TCGATGAGCT CCAAGATCAT TGGTGCTGAC GCAGAGTTCT TCTCAGCCAT GGTTGTGGAC
0481 GCTGCCCAGT CGGTGAAAAT C |
| in silico PCR Fragment |
0001 CATCGGTGCG CACACAGAAC GTTATGGCCG CTCTGTCCAT ATCGAACATC GTGAAAAGCT
0061 CCTTGGGACC CGTGGGTCTG -GACAAGATG CTGGTGGACG ACATCGGCGA TGTCACAGTG
0121 ACCAACGATG GAGCCACCAT TCTGCGACTG CTGGAGGTGG AGCACCCGGC AGCCAAGGTC
0181 CTGGTTGAGC TGGCTCAGCT GCAGGACGAG GAGGTCGGCG ATGGCACTAC CTCGGTGGTT
0241 ATCCTGGCCG CTGAGCTGCT GAAAAACGCC GACGAGCTGG TCAAGCAGAA GATTCATCCT
0301 ACCTCCATAA TCTCCGGCTA CCGCATTGCC TGCAAGGAGG CCTGCAAGTA CATCTCGGAG
0361 CATCTGACCG CTCCCGTTGA TGAGCTGGGC CGCGATTCCC TGATTAACAT TGCCAAGACC
0421 TCGATGAGCT CCAAGATCAT TGGTGCTGAC GCAGAGTTCT TCTCAGCCAT GGTTGTGGAC
0481 GCTGCCCAGT CGGTGAAAAT C |
| Assemble Data |
<PRE>
5374R-2.IR_full 1 CATCGGTGCGCACACAGAACGTTATGGCCGCTCTGTCCATATCGAACATCGTGAAAAGCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5374R-2.in silico 1 CATCGGTGCGCACACAGAACGTTATGGCCGCTCTGTCCATATCGAACATCGTGAAAAGCT 60
5374R-2.IR_full 61 CCTTGGGACCCGTGGGTCTG<span class="snp"><tt>A</tt></span>GACAAGATGCTGGTGGACGACATCGGCGATGTCACAGTG 120
||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||
5374R-2.in silico 61 CCTTGGGACCCGTGGGTCTG<span class="snp"><tt>-</tt></span>GACAAGATGCTGGTGGACGACATCGGCGATGTCACAGTG 120
5374R-2.IR_full 121 ACCAACGATGGAGCCACCATTCTGCGACTGCTGGAGGTGGAGCACCCGGCAGCCAAGGTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5374R-2.in silico 121 ACCAACGATGGAGCCACCATTCTGCGACTGCTGGAGGTGGAGCACCCGGCAGCCAAGGTC 180
5374R-2.IR_full 181 CTGGTTGAGCTGGCTCAGCTGCAGGACGAGGAGGTCGGCGATGGCACTACCTCGGTGGTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5374R-2.in silico 181 CTGGTTGAGCTGGCTCAGCTGCAGGACGAGGAGGTCGGCGATGGCACTACCTCGGTGGTT 240
5374R-2.IR_full 241 ATCCTGGCCGCTGAGCTGCTGAAAAACGCCGACGAGCTGGTCAAGCAGAAGATTCATCCT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5374R-2.in silico 241 ATCCTGGCCGCTGAGCTGCTGAAAAACGCCGACGAGCTGGTCAAGCAGAAGATTCATCCT 300
5374R-2.IR_full 301 ACCTCCATAATCTCCGGCTACCGCATTGCCTGCAAGGAGGCCTGCAAGTACATCTCGGAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5374R-2.in silico 301 ACCTCCATAATCTCCGGCTACCGCATTGCCTGCAAGGAGGCCTGCAAGTACATCTCGGAG 360
5374R-2.IR_full 361 CATCTGACCGCTCCCGTTGATGAGCTGGGCCGCGATTCCCTGATTAACATTGCCAAGACC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5374R-2.in silico 361 CATCTGACCGCTCCCGTTGATGAGCTGGGCCGCGATTCCCTGATTAACATTGCCAAGACC 420
5374R-2.IR_full 421 TCGATGAGCTCCAAGATCATTGGTGCTGACGCAGAGTTCTTCTCAGCCATGGTTGTGGAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5374R-2.in silico 421 TCGATGAGCTCCAAGATCATTGGTGCTGACGCAGAGTTCTTCTCAGCCATGGTTGTGGAC 480
5374R-2.IR_full 481 GCTGCCCAGTCGGTGAAAATC 501
|||||||||||||||||||||
5374R-2.in silico 481 GCTGCCCAGTCGGTGAAAATC 501
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079726.3 |
CG5374-RA, transcript variant A (T-cp1), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_170016.1 |
CG5374-RB, transcript variant B (T-cp1), mRNA |
| 0.2 |
1 |
4 |
2 |
4 |
NM_134910.2 |
CG18555-RA (CG18555), mRNA |
| 0.2 |
1 |
2 |
5 |
12 |
NM_141578.1 |
CG8351-RA (CG8351), mRNA |
| 0 |
0 |
13 |
23 |
15 |
NM_167176.1 |
CG7033-RB, transcript variant B (CG7033), mRNA |
| 0 |
0 |
13 |
23 |
15 |
NM_132296.2 |
CG7033-RA, transcript variant A (CG7033), mRNA |
| 0 |
0 |
13 |
23 |
15 |
NM_176715.1 |
CG7033-RC, transcript variant C (CG7033), mRNA |
| 0 |
0 |
5 |
16 |
36 |
NM_078983.2 |
CG8439-RA, transcript variant A (Cct5), mRNA |
| 0 |
0 |
5 |
16 |
36 |
NM_165867.1 |
CG8439-RB, transcript variant B (Cct5), mRNA |
| 0 |
0 |
4 |
4 |
6 |
NM_078866.2 |
CG6549-RA, transcript variant A (fws), mRNA |
| 0 |
0 |
4 |
4 |
6 |
NM_165221.1 |
CG6549-RC, transcript variant C (fws), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_143567.3 |
CG11315-RA, transcript variant A (CG11315), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_206238.1 |
CG13923-RA (CG13923), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_132998.1 |
CG8326-RA (CG8326), mRNA |
| 0 |
0 |
2 |
9 |
13 |
NM_142315.2 |
CG8977-RA, transcript variant A (Cctgamma), mRNA |
| 0 |
0 |
2 |
9 |
13 |
NM_169727.1 |
CG8977-RB, transcript variant B (Cctgamma), mRNA |
| 0 |
0 |
2 |
6 |
24 |
NM_135735.1 |
CG5525-RA (CG5525), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_137249.1 |
CG18243-RA (Ptp52F), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_135751.2 |
CG5075-RA (CG5075), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_131937.2 |
CG3564-RA (CHOp24), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_136727.2 |
CG18408-RA, transcript variant A (CAP), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_165758.1 |
CG18408-RF, transcript variant F (CAP), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_165757.1 |
CG18408-RC, transcript variant C (CAP), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139810.2 |
CG9953-RA (CG9953), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_167908.1 |
CG7961-RB, transcript variant B (alphaCop), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_058047.3 |
CG7961-RA, transcript variant A (alphaCop), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139421.3 |
CG5687-RA (CG5687), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165762.1 |
CG18408-RE, transcript variant E (CAP), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165761.1 |
CG18408-RD, transcript variant D (CAP), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165760.2 |
CG18408-RB, transcript variant B (CAP), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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