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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5178R-1 
 Symbol Act88F  Full Name Actin 88F 
 CG No CG5178  Old CG No CG5178 
 Synonyms Actin, actin, E, act88F, CG5178, Act(88F), act 88F, Act88-F, Ifm(3)7, rsd, Act88F 
 Accession No (Link to NCBI) NM_079643.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002 [ PubMed ID = 25977760 ] [ RRC reference ]

Fairchild MJ, Islam F, Tanentzapf G.
Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
PLoS Genet. (2017) 13(9) e1007026 [ PubMed ID = 28957323 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCTCCGGTCGTACCACCGGTAT 
 Primer Seq. 3'
aatctagaggtaccTACCGCCGGACAGCACAGAGT 
 Predicted Fragment Size
476 
 IR fragment full Seq
0001 CTCCgGTCGT ACCACCGGTA TTGTGCTGGA CTCCGGCGAT GGTGTCTCCC ACACCGTGCC 
0061 CATCTATGAG GGCTTcgcCC NGCNCCCACG CCATTCTGCG TCTGGATCTG GCTGGTCGCG 
0121 ATCTGACCGA TTACCTGATG AAGATCCTGA CGGAGCGCGG CTACAGCNTT CACCACCACC 
0181 GCCGAGCGTG AGATCGTGCG CGACATCAAG GAGAAGCTGT GCTACGTGGC TCTGGACTTC 
0241 GAGCAGGAGA TGGCCACCGC TGCCGCCTCC ACCTCGCTGG AGAAGTCGTA CGAGTTGCCT 
0301 GACGGCCAGG TGATCACCAT TGGCAANCGA GCGCTTCCGC TGCCCCGAGG CTCTGTTCCA 
0361 GCCCTCGTTC CTGGGCATGG AGTCGTGCGG CATCCACGAG ACCGTCTACA ACTCGATCAT 
0421 GAAGTGCGAC GTGGACATCC GCAAGGATCT GTATGCCAAC TCTGTGCTGT NCGGCGGTA 
 in silico PCR Fragment
0001 CTCCGGTCGT ACCACCGGTA TTGTGCTGGA CTCCGGCGAT GGTGTCTCCC ACACCGTGCC 
0061 CATCTATGAG GGCTTCGCCC TGC-CCCACG CCATTCTGCG TCTGGATCTG GCTGGTCGCG 
0121 ATCTGACCGA TTACCTGATG AAGATCCTGA CGGAGCGCGG CTACAGC-TT CACCACCACC 
0181 GCCGAGCGTG AGATCGTGCG CGACATCAAG GAGAAGCTGT GCTACGTGGC TCTGGACTTC 
0241 GAGCAGGAGA TGGCCACCGC TGCCGCCTCC ACCTCGCTGG AGAAGTCGTA CGAGTTGCCT 
0301 GACGGCCAGG TGATCACCAT TGGCAA-CGA GCGCTTCCGC TGCCCCGAGG CTCTGTTCCA 
0361 GCCCTCGTTC CTGGGCATGG AGTCGTGCGG CATCCACGAG ACCGTCTACA ACTCGATCAT 
0421 GAAGTGCGAC GTGGACATCC GCAAGGATCT GTATGCCAAC TCTGTGCTGT CCGGCGGTA 
 Assemble Data

5178R-1.IR_full       1   CTCCGGTCGTACCACCGGTATTGTGCTGGACTCCGGCGATGGTGTCTCCCACACCGTGCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5178R-1.in silico     1   CTCCGGTCGTACCACCGGTATTGTGCTGGACTCCGGCGATGGTGTCTCCCACACCGTGCC 60


5178R-1.IR_full       61  CATCTATGAGGGCTTCGCCCNGCNCCCACGCCATTCTGCGTCTGGATCTGGCTGGTCGCG 120
                          |||||||||||||||||||| || ||||||||||||||||||||||||||||||||||||
5178R-1.in silico     61  CATCTATGAGGGCTTCGCCCTGC-CCCACGCCATTCTGCGTCTGGATCTGGCTGGTCGCG 120


5178R-1.IR_full       121 ATCTGACCGATTACCTGATGAAGATCCTGACGGAGCGCGGCTACAGCNTTCACCACCACC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
5178R-1.in silico     121 ATCTGACCGATTACCTGATGAAGATCCTGACGGAGCGCGGCTACAGC-TTCACCACCACC 180


5178R-1.IR_full       181 GCCGAGCGTGAGATCGTGCGCGACATCAAGGAGAAGCTGTGCTACGTGGCTCTGGACTTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5178R-1.in silico     181 GCCGAGCGTGAGATCGTGCGCGACATCAAGGAGAAGCTGTGCTACGTGGCTCTGGACTTC 240


5178R-1.IR_full       241 GAGCAGGAGATGGCCACCGCTGCCGCCTCCACCTCGCTGGAGAAGTCGTACGAGTTGCCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5178R-1.in silico     241 GAGCAGGAGATGGCCACCGCTGCCGCCTCCACCTCGCTGGAGAAGTCGTACGAGTTGCCT 300


5178R-1.IR_full       301 GACGGCCAGGTGATCACCATTGGCAANCGAGCGCTTCCGCTGCCCCGAGGCTCTGTTCCA 360
                          |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
5178R-1.in silico     301 GACGGCCAGGTGATCACCATTGGCAA-CGAGCGCTTCCGCTGCCCCGAGGCTCTGTTCCA 360


5178R-1.IR_full       361 GCCCTCGTTCCTGGGCATGGAGTCGTGCGGCATCCACGAGACCGTCTACAACTCGATCAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5178R-1.in silico     361 GCCCTCGTTCCTGGGCATGGAGTCGTGCGGCATCCACGAGACCGTCTACAACTCGATCAT 420


5178R-1.IR_full       421 GAAGTGCGACGTGGACATCCGCAAGGATCTGTATGCCAACTCTGTGCTGTNCGGCGGTA 479
                          |||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
5178R-1.in silico     421 GAAGTGCGACGTGGACATCCGCAAGGATCTGTATGCCAACTCTGTGCTGTCCGGCGGTA 479

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   458  NM_079643.1  CG5178-RA (Act88F), mRNA 
19.65   90  94  184  70  NM_079486.3  CG7478-RA (Act79B), mRNA 
10.48   48  119  134  78  NM_001014726.1  CG4027-RC, transcript variant C (Act5C), mRNA 
10.48   48  119  134  78  NM_001014725.1  CG4027-RD, transcript variant D (Act5C), mRNA 
10.48   48  119  134  78  NM_167053.1  CG4027-RA, transcript variant A (Act5C), mRNA 
10.48   48  119  134  78  NM_078497.2  CG4027-RB, transcript variant B (Act5C), mRNA 
9.82   45  150  125  82  NM_057743.3  CG18290-RA, transcript variant A (Act87E), mRNA 
9.82   45  150  125  82  NM_169525.1  CG18290-RB, transcript variant B (Act87E), mRNA 
8.73   40  50  157  119  NM_078901.2  CG12051-RA (Act42A), mRNA 
7.64   35  137  106  105  NM_166414.1  CG10067-RB, transcript variant B (Act57B), mRNA 
7.64   35  137  106  105  NM_079076.3  CG10067-RA, transcript variant A (Act57B), mRNA 
0.65   22  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
0.65   22  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
0.65   22  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
0   NM_144354.2  CG6987-RA (SF2), mRNA 
0   10  NM_132320.2  CG12124-RA (CG12124), mRNA 
0   NM_001031917.1  CG1414-RB, transcript variant B (bbx), mRNA 
0   NM_001031918.1  CG1410-RA, transcript variant A (waw), mRNA 
0   NM_001031916.1  CG1414-RC, transcript variant C (bbx), mRNA 
0   NM_139766.1  CG6619-RA (CG6619), mRNA 
0   20  46  NM_057689.1  CG5409-RA (Arp53D), mRNA 
0   NM_078648.2  CG9901-RA (Arp14D), mRNA 
0   10  NM_057862.3  CG17246-RA, transcript variant A (Scs-fp), mRNA 
0   10  NM_166343.1  CG17246-RB, transcript variant B (Scs-fp), mRNA 
0   10  NM_166344.1  CG17246-RC, transcript variant C (Scs-fp), mRNA 
0   NM_136415.1  CG12835-RA (CG12835), mRNA 
0   13  NM_168755.1  CG8127-RB, transcript variant B (Eip75B), mRNA 
0   NM_167020.1  CG6986-RB, transcript variant B (CG6986), mRNA 
0   NM_001014724.1  CG6986-RC, transcript variant C (CG6986), mRNA 
0   NM_131955.1  CG6986-RA, transcript variant A (CG6986), mRNA 

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