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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5123R-2 |
| Symbol |
W |
Full Name |
Wrinkled |
| CG No |
CG5123 |
Old CG No |
CG5123 |
| Synonyms |
hid, Hid, Hid1, HID, CG5123, l(3)05014, W |
| Accession No (Link to NCBI) |
NM_079412.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
male lethal, female abnormal notum/wing |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Khammari A, Agnès F, Gandille P, Pret AM.
Physiological apoptosis of polar cells during Drosophila oogenesis is mediated by Hid-dependent regulation of Diap1.
Cell Death Differ (2011) 18(5) 793-805
[
PubMed ID = 21113144
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCCGTGCCCTTTTATTTGCCC |
| Primer Seq. 3' |
aatctagaggtaccTGCTGCTGCCGGAAGAAGTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCCGTGCCCT TTTATTTGCC CGAGGGCGGC GCCGATGACG TAGCGTCGAG TTCATCGGGA
0061 GCCTCGGGCA ACTCCTCCCC CCACAACCAC CCACTTCCCT CGAGCGCATC CTCGTCCGTC
0121 TCCTCCTCGG GCGTGTCCTC GGCCTCCGCC TCCTCGGCCT CATCTTCGTC ATCCGCATCG
0181 TCGGACGGCG CCAGCAGCGC CGCCTCGCAA TCGCCGAACA CCACCACCTC GTCGGCCACG
0241 CAGACGCACG ATGCAGTCTC CACTGCCCAC CGACCAAGTG CTATACGCCC TCTACGAGTG
0301 GGTCAGGATG TACCAGAGCC AGCAGAGTGC CCCGCAAATC TTCCAGTATC CGCCGCCAAG
0361 CCCCTCTTGC AATTTCACTG GCGGCGATGT GTTCTTTCCG CACGGCCATC CGAATCCGAA
0421 CTCGAATCCC CATCCGCGCA CCCCCCGAAC CAGCGTGAGC TTCTcCTCCG GCGAGGAGTA
0481 CAACTTCTTC CGGCAGCAGC A |
| in silico PCR Fragment |
0001 GCCGTGCCCT TTTATTTGCC CGAGGGCGGC GCCGATGACG TAGCGTCGAG TTCATCGGGA
0061 GCCTCGGGCA ACTCCTCCCC CCACAACCAC CCACTTCCCT CGAGCGCATC CTCGTCCGTC
0121 TCCTCCTCGG GCGTGTCCTC GGCCTCCGCC TCCTCGGCCT CATCTTCGTC CTCCGCATCG
0181 TCGGACGGCG CCAGCAGCGC CGCCTCGCAA TCGCCGAACA CCACCACCTC GTCGGCCACG
0241 CAGACGC-CG ATGCAGTCTC CACTGCCCAC CGACCAAGTG CTATACGCCC TCTACGAGTG
0301 GGTCAGGATG TACCAGAGCC AGCAGAGTGC CCCGCAAATC TTCCAGTATC CGCCGCCAAG
0361 CCCCTCTTGC AATTTCACGG GCGGCGATGT GTTCTTTCCG CACGGCCATC CGAATCCGAA
0421 CTCGAATCCC CATCCGCGCA CCCCCCGAAC CAGCGTGAGC TTCTCCTCCG GCGAGGAGTA
0481 CAACTTCTTC CGGCAGCAGC A |
| Assemble Data |
5123R-2.IR_full 1 GCCGTGCCCTTTTATTTGCCCGAGGGCGGCGCCGATGACGTAGCGTCGAGTTCATCGGGA 60
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5123R-2.in silico 1 GCCGTGCCCTTTTATTTGCCCGAGGGCGGCGCCGATGACGTAGCGTCGAGTTCATCGGGA 60
5123R-2.IR_full 61 GCCTCGGGCAACTCCTCCCCCCACAACCACCCACTTCCCTCGAGCGCATCCTCGTCCGTC 120
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5123R-2.in silico 61 GCCTCGGGCAACTCCTCCCCCCACAACCACCCACTTCCCTCGAGCGCATCCTCGTCCGTC 120
5123R-2.IR_full 121 TCCTCCTCGGGCGTGTCCTCGGCCTCCGCCTCCTCGGCCTCATCTTCGTCATCCGCATCG 180
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5123R-2.in silico 121 TCCTCCTCGGGCGTGTCCTCGGCCTCCGCCTCCTCGGCCTCATCTTCGTCCTCCGCATCG 180
5123R-2.IR_full 181 TCGGACGGCGCCAGCAGCGCCGCCTCGCAATCGCCGAACACCACCACCTCGTCGGCCACG 240
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5123R-2.in silico 181 TCGGACGGCGCCAGCAGCGCCGCCTCGCAATCGCCGAACACCACCACCTCGTCGGCCACG 240
5123R-2.IR_full 241 CAGACGCACGATGCAGTCTCCACTGCCCACCGACCAAGTGCTATACGCCCTCTACGAGTG 300
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5123R-2.in silico 241 CAGACGC-CGATGCAGTCTCCACTGCCCACCGACCAAGTGCTATACGCCCTCTACGAGTG 300
5123R-2.IR_full 301 GGTCAGGATGTACCAGAGCCAGCAGAGTGCCCCGCAAATCTTCCAGTATCCGCCGCCAAG 360
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5123R-2.in silico 301 GGTCAGGATGTACCAGAGCCAGCAGAGTGCCCCGCAAATCTTCCAGTATCCGCCGCCAAG 360
5123R-2.IR_full 361 CCCCTCTTGCAATTTCACTGGCGGCGATGTGTTCTTTCCGCACGGCCATCCGAATCCGAA 420
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5123R-2.in silico 361 CCCCTCTTGCAATTTCACGGGCGGCGATGTGTTCTTTCCGCACGGCCATCCGAATCCGAA 420
5123R-2.IR_full 421 CTCGAATCCCCATCCGCGCACCCCCCGAACCAGCGTGAGCTTCTCCTCCGGCGAGGAGTA 480
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5123R-2.in silico 421 CTCGAATCCCCATCCGCGCACCCCCCGAACCAGCGTGAGCTTCTCCTCCGGCGAGGAGTA 480
5123R-2.IR_full 481 CAACTTCTTCCGGCAGCAGCA 501
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5123R-2.in silico 481 CAACTTCTTCCGGCAGCAGCA 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
6 |
NM_079412.2 |
CG5123-RA (W), mRNA |
| 0 |
0 |
7 |
8 |
23 |
NM_176460.1 |
CG31374-RC, transcript variant C (CG31374), mRNA |
| 0 |
0 |
7 |
8 |
23 |
NM_169390.2 |
CG31374-RB, transcript variant B (CG31374), mRNA |
| 0 |
0 |
7 |
4 |
15 |
NM_169391.1 |
CG31374-RA, transcript variant A (CG31374), mRNA |
| 0 |
0 |
4 |
9 |
32 |
NM_132831.1 |
CG8184-RB (CG8184), mRNA |
| 0 |
0 |
4 |
4 |
16 |
NM_132908.2 |
CG4453-RA (Nup153), mRNA |
| 0 |
0 |
4 |
4 |
15 |
NM_139508.2 |
CG32486-RD (CG32486), mRNA |
| 0 |
0 |
4 |
3 |
9 |
NM_135755.1 |
CG9932-RA (CG9932), mRNA |
| 0 |
0 |
3 |
6 |
14 |
NM_001038902.1 |
CG33993-RA (CG33993), mRNA |
| 0 |
0 |
3 |
5 |
17 |
NM_167210.1 |
CG32694-RC, transcript variant C (CG32694), mRNA |
| 0 |
0 |
3 |
5 |
17 |
NM_167209.1 |
CG32694-RA, transcript variant A (CG32694), mRNA |
| 0 |
0 |
3 |
5 |
6 |
NM_136882.2 |
CG30040-RA (jeb), mRNA |
| 0 |
0 |
3 |
3 |
7 |
NM_001015362.1 |
CG41101-PA.3 (CG41101), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_141590.2 |
CG11964-RA (CG11964), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_143422.1 |
CG11898-RA (CG11898), mRNA |
| 0 |
0 |
2 |
5 |
47 |
NM_080025.1 |
CG11387-RA, transcript variant A (ct), mRNA |
| 0 |
0 |
2 |
4 |
15 |
NM_166126.1 |
CG18255-RC, transcript variant C (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_139725.2 |
CG5249-RA (Blimp-1), mRNA |
| 0 |
0 |
2 |
4 |
13 |
NM_166130.1 |
CG18255-RE, transcript variant E (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_134752.2 |
CG5561-RA (CG5561), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_166129.2 |
CG18255-RD, transcript variant D (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
3 |
20 |
NM_001038965.1 |
CG9924-RE, transcript variant E (CG9924), mRNA |
| 0 |
0 |
2 |
3 |
15 |
NM_057773.3 |
CG7538-RA (Mcm2), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_078689.2 |
CG14228-RA (Mer), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_164605.1 |
CG8886-RB, transcript variant B (l(2)05714), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143776.3 |
CG8886-RA, transcript variant A (l(2)05714), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_001014630.1 |
CG33547-RA (Rim), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_169046.1 |
CG31538-RA (CG31538), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_143281.1 |
CG6066-RA (CG6066), mRNA |
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