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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5092R-2 |
| Symbol |
Tor |
Full Name |
Target of rapamycin |
| CG No |
CG5092 |
Old CG No |
CG5092 |
| Synonyms |
TOR, dTOR, mTOR, CG5092, dTor, dtor, unnamed, FRAP/TOR, CT24817, CT24745, CT16317, l(2)k17004, Tor, tor |
| Accession No (Link to NCBI) |
NM_080152.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Matsuka M, Otsune S, Sugimori S, Tsugita Y, Ueda H, Nakagoshi H.<br> Fecundity is optimized by levels of nutrient signal-dependent expression of Dve and EcR in Drosophila male accessory gland.<br> Dev Biol (2024) 508 8-23
[
PubMed ID = 38199580
]
[
RRC reference
]
Ostalé CM, Esteban N, López-Varea A, de Celis JF.<br> Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.<br> G3 (Bethesda) (2021) 11(12)
[
PubMed ID = 34599799
]
[
RRC reference
]
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.<br> ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.<br> Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.<br> A Kinome RNAi Screen in <i>Drosophila</i> Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.<br> G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Hosono C, Matsuda R, Adryan B, Samakovlis C.<br> Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.<br> Nat Cell Biol (2015) 17(12) 1569-76
[
PubMed ID = 26551273
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.<br> Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.<br> Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Resnik-Docampo M, de Celis JF.<br> MAP4K3 is a component of the TORC1 signalling complex that modulates cell growth and viability in Drosophila melanogaster.<br> PLoS One (2011) 6(1) e14528
[
PubMed ID = 21267071
]
[
RRC reference
]
Willecke M, Toggweiler J, Basler K.<br> Loss of PI3K blocks cell-cycle progression in a Drosophila tumor model.<br> Oncogene (2011) 30(39) 4067-74
[
PubMed ID = 21516128
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGTCGACGACGTCGGTGGTG |
| Primer Seq. 3' |
aatctagaggtaccGGCAAGGCGATAGCCAGCTCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGTCGACgA CGTCGGTGGT GCAGCAGTTC GTCAACGGGC TTAAGTCCCG CAACCGGAAT
0061 GTACAGAACA AGGCAACCCA GGATCTGCTC TTCTATGTGA AGACCGAGCT ACGCGAAATG
0121 TCCCAGGAGG AGCTGGCCCA ATTCTTCGAC GAGTTCGACC ATCACATTTT CACGATGGTG
0181 AATGCGACAG ATATCAACGA GAAAAAAGGC GGTGCCCTGG CCATGAAGTG CCTGATTAAC
0241 TGCGAGGGCA GTCTTACTGC CAGGAAGGGC ATTTCGCCCT ATCTCAACCG CCTGCGCGAT
0301 CTTCTGCTAA TCAACGATGT GTCCGTCATG GAGATTGCCG CCCGCTCCCT GGTCAAATTG
0361 GCCAACATGC CCACCTCCAA GGGCGCCGAT TCCTTCGACT TTGATATTAA GAAGGCTTTT
0421 GAGGTGCTCA GAGGCGAGAG ACAAGAGTAC CGCCGCCATT CAGCCGTCTT TATCCTCCGT
0481 GAGCTGGCTA TCGCCTTGCC |
| in silico PCR Fragment |
0001 ATGTCGACGA CGTCGGTGGT GCAGCAGTTC GTCAACGGGC TTAAGTCCCG CAACCGGAAT
0061 GTACAGAACA AGGCAACCCA GGATCTGCTC TTCTATGTGA AGACCGAGCT ACGCGAAATG
0121 TCCCAGGAGG AGCTGGCCCA ATTTTTCGAC GAGTTCGACC ATCACATTTT CACGATGGTG
0181 AATGCGACAG ATATCAACGA GAAAAAAGGC GGTGCCCTGG CCATGAAGTG CCTGATTAAC
0241 TGCGAGGGCA GTCTTACTGC CAGGAAGGGC ATTTCGCCCT ATCTCAACCG CCTGCGCGAT
0301 CTTCTGCTAA TCAACGATGT GTCCGTCATG GAGATTGCCG CCCGCTCCCT GGTCAAATTG
0361 GCCAACATGC CCACCTCCAA GGGCGCCGAT TCCTTCGACT TTGATATTAA GAAGGCTTTT
0421 GAGGTGCTCA GAGGCGAGAG ACAAGAGTAC CGCCGCCATT CAGCCGTCTT TATCCTCCGT
0481 GAGCTGGCTA TCGCCTTGCC |
| Assemble Data |
<PRE>
5092R-2.IR_full 1 ATGTCGACGACGTCGGTGGTGCAGCAGTTCGTCAACGGGCTTAAGTCCCGCAACCGGAAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5092R-2.in silico 1 ATGTCGACGACGTCGGTGGTGCAGCAGTTCGTCAACGGGCTTAAGTCCCGCAACCGGAAT 60
5092R-2.IR_full 61 GTACAGAACAAGGCAACCCAGGATCTGCTCTTCTATGTGAAGACCGAGCTACGCGAAATG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5092R-2.in silico 61 GTACAGAACAAGGCAACCCAGGATCTGCTCTTCTATGTGAAGACCGAGCTACGCGAAATG 120
5092R-2.IR_full 121 TCCCAGGAGGAGCTGGCCCAATT<span class="snp"><tt>C</tt></span>TTCGACGAGTTCGACCATCACATTTTCACGATGGTG 180
|||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||
5092R-2.in silico 121 TCCCAGGAGGAGCTGGCCCAATT<span class="snp"><tt>T</tt></span>TTCGACGAGTTCGACCATCACATTTTCACGATGGTG 180
5092R-2.IR_full 181 AATGCGACAGATATCAACGAGAAAAAAGGCGGTGCCCTGGCCATGAAGTGCCTGATTAAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5092R-2.in silico 181 AATGCGACAGATATCAACGAGAAAAAAGGCGGTGCCCTGGCCATGAAGTGCCTGATTAAC 240
5092R-2.IR_full 241 TGCGAGGGCAGTCTTACTGCCAGGAAGGGCATTTCGCCCTATCTCAACCGCCTGCGCGAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5092R-2.in silico 241 TGCGAGGGCAGTCTTACTGCCAGGAAGGGCATTTCGCCCTATCTCAACCGCCTGCGCGAT 300
5092R-2.IR_full 301 CTTCTGCTAATCAACGATGTGTCCGTCATGGAGATTGCCGCCCGCTCCCTGGTCAAATTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5092R-2.in silico 301 CTTCTGCTAATCAACGATGTGTCCGTCATGGAGATTGCCGCCCGCTCCCTGGTCAAATTG 360
5092R-2.IR_full 361 GCCAACATGCCCACCTCCAAGGGCGCCGATTCCTTCGACTTTGATATTAAGAAGGCTTTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5092R-2.in silico 361 GCCAACATGCCCACCTCCAAGGGCGCCGATTCCTTCGACTTTGATATTAAGAAGGCTTTT 420
5092R-2.IR_full 421 GAGGTGCTCAGAGGCGAGAGACAAGAGTACCGCCGCCATTCAGCCGTCTTTATCCTCCGT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5092R-2.in silico 421 GAGGTGCTCAGAGGCGAGAGACAAGAGTACCGCCGCCATTCAGCCGTCTTTATCCTCCGT 480
5092R-2.IR_full 481 GAGCTGGCTATCGCCTTGCC 500
||||||||||||||||||||
5092R-2.in silico 481 GAGCTGGCTATCGCCTTGCC 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_080152.2 |
CG5092-RA (Tor), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_143634.2 |
CG2191-RA (CG2191), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_132678.2 |
CG2691-RA (CG2691), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_134737.2 |
CG5118-RA (CG5118), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141877.1 |
CG18347-RA (CG18347), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_170183.2 |
CG11120-RB, transcript variant B (CG11120), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143055.2 |
CG11120-RA, transcript variant A (CG11120), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_137864.1 |
CG30259-RA (CG30259), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_165312.1 |
CG10263-RC, transcript variant C (CG10263), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_136149.2 |
CG10263-RA, transcript variant A (CG10263), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_176061.2 |
CG10263-RD, transcript variant D (CG10263), mRNA |
| 0 |
0 |
1 |
4 |
13 |
NM_132370.1 |
CG2989-RA (CG2989), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132217.2 |
CG10958-RA (CG10958), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_080325.3 |
CG6450-RC (lva), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142106.1 |
CG14843-RA (CG14843), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168955.1 |
CG6838-RB, transcript variant B (CG6838), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141152.1 |
CG6838-RA, transcript variant A (CG6838), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142573.1 |
CG11391-RA (CG11391), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_132036.1 |
CG15767-RA (CG15767), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135181.1 |
CG13982-RA (CG13982), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143685.2 |
CG11062-RA (activin-beta), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_136422.2 |
CG1845-RA (CG1845), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_135765.3 |
CG5458-RA (CG5458), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_138187.2 |
CG1228-RC, transcript variant C (Ptpmeg), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167830.1 |
CG1228-RA, transcript variant A (Ptpmeg), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167832.2 |
CG1228-RB, transcript variant B (Ptpmeg), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167831.1 |
CG1228-RD, transcript variant D (Ptpmeg), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_170158.1 |
CG5794-RB, transcript variant B (CG5794), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_143018.2 |
CG5794-RD, transcript variant D (CG5794), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135239.1 |
CG18304-RA (CG18304), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04136
:
Autophagy - other
dme04140
:
Autophagy - animal
dme04150
:
mTOR signaling pathway
dme04213
:
Longevity regulating pathway - multiple species
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