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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5055R-1 
 Symbol baz  Full Name bazooka 
 CG No CG5055  Old CG No CG5055 
 Synonyms baz, Par-3, Baz, par-3, PAR3, Par3, dPar-3, l(1)G0484, D-Par3, CG5055, Baz/Par-3, PAR-3 
 Accession No (Link to NCBI) NM_078659.3 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Diaz-de-la-Loza MD, Ray RP, Ganguly PS, Alt S, Davis JR, Hoppe A, Tapon N, Salbreux G, Thompson BJ.
Apical and Basal Matrix Remodeling Control Epithelial Morphogenesis.
Dev. Cell (2018) 46(1) 23-39.e5 [ PubMed ID = 29974861 ] [ RRC reference ]

Jones TA, Metzstein MM.
A novel function for the PAR complex in subcellular morphogenesis of tracheal terminal cells in Drosophila melanogaster.
Genetics (2011) 189(1) 153-64 [ PubMed ID = 21750259 ] [ RRC reference ]

Wang H, Qiu Z, Xu Z, Chen SJ, Luo J, Wang X, Chen J.
aPKC is a key polarity determinant in coordinating the function of three distinct cell polarities during collective migration.
Development (2018) 145(9) [ PubMed ID = 29636381 ] [ RRC reference ]

Wang Y, Antunes M, Anderson AE, Kadrmas JL, Jacinto A, Galko MJ.
Integrin Adhesions Suppress Syncytium Formation in the Drosophila Larval Epidermis.
Curr. Biol. (2015) 25(17) 2215-27 [ PubMed ID = 26255846 ] [ RRC reference ]

Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92 [ PubMed ID = 19363474 ] [ RRC reference ]

Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgTGAAGGTCACCGTCTGCTTCG 
 Primer Seq. 3'
aatctagaggtaccGCGTTTGTTACTGCCCTCTGC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 TGAAGGTCAC CGTCTGCTTC GGCGACGTTC GCATTCTGGT TCCCTGTGGT TCCGGCGAAC 
0061 TCCTCGTCCG CGATCTCGTC AAGGAGGCCA CGCGACGCTA CATCAAAGGC GGCCGGCAAG 
0121 CCCCGATTCC TGGGTGACCG TGACGCATCT CCAGACACAG TCGGGCATCC TCGATCCGGA 
0181 TGATTGTGTC CGCGACGTGG CCGACGATCG GGAGCAGATA TTGGCGCACT TTGATGACCC 
0241 AGGACCCGAT CCAGGAGTTC CGCAAGGAGG CGGAGATGGA GCATCGGGCA GTTCGTCCGT 
0301 TGGCACCGGT TCGCCGGACA TCTTTCGCGA TCCCACCAAC ACGGAGGCGC CCACCTGTCC 
0361 GCGGGATCTC TCCACGCCAC ACATCGAGGT CACCAGCACC ACATCGGGAC CGATGGCTGG 
0421 ACTCGGAGTT GGACTGATGG TGCGTCGCAG CAGTGATCCC AATCTGCTGG CCTCGCTGAA 
0481 GGCAGAGGGC AGTAACAAAC GC 
 in silico PCR Fragment
0001 TGAAGGTCAC CGTCTGCTTC GGCGACGTTC GCATTCTGGT TCCCTGTGGT TCCGGCGAAC 
0061 TCCTCGTCCG CGATCTCGTC AAGGAGGCCA CGCGACGCTA CATC-AAGGC GGCCGGCAAG 
0121 -CCCGATTCC TGGGTGACCG TGACGCATCT CCAGACACAG TCGGGCATCC TCGATCCGGA 
0181 TGATTGTGTC CGCGACGTGG CCGACGATCG GGAGCAGATA TTGGCGCACT TTGATGACCC 
0241 AGGACCCGAT CCAGGAGTTC CGCAAGGAGG CGGAGATGGA GCATCGGGCA GTTCGTCCGT 
0301 TGGCACCGGT TCGCCGGACA TCTTTCGCGA TCCCACCAAC ACGGAGGCGC CCACCTGTCC 
0361 GCGGGATCTC TCCACGCCAC ACATCGAGGT CACCAGCACC ACATCGGGAC CGATGGCTGG 
0421 ACTCGGAGTT GGACTGATGG TGCGTCGCAG CAGTGATCCC AATCTGCTGG CCTCGCTGAA 
0481 GGCAGAGGGC AGTAACAAAC GC 
 Assemble Data

5055R-1.IR_full       1   TGAAGGTCACCGTCTGCTTCGGCGACGTTCGCATTCTGGTTCCCTGTGGTTCCGGCGAAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5055R-1.in silico     1   TGAAGGTCACCGTCTGCTTCGGCGACGTTCGCATTCTGGTTCCCTGTGGTTCCGGCGAAC 60


5055R-1.IR_full       61  TCCTCGTCCGCGATCTCGTCAAGGAGGCCACGCGACGCTACATCAAAGGCGGCCGGCAAG 120
                          |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
5055R-1.in silico     61  TCCTCGTCCGCGATCTCGTCAAGGAGGCCACGCGACGCTACATC-AAGGCGGCCGGCAAG 120


5055R-1.IR_full       121 CCCCGATTCCTGGGTGACCGTGACGCATCTCCAGACACAGTCGGGCATCCTCGATCCGGA 180
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5055R-1.in silico     121 -CCCGATTCCTGGGTGACCGTGACGCATCTCCAGACACAGTCGGGCATCCTCGATCCGGA 180


5055R-1.IR_full       181 TGATTGTGTCCGCGACGTGGCCGACGATCGGGAGCAGATATTGGCGCACTTTGATGACCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5055R-1.in silico     181 TGATTGTGTCCGCGACGTGGCCGACGATCGGGAGCAGATATTGGCGCACTTTGATGACCC 240


5055R-1.IR_full       241 AGGACCCGATCCAGGAGTTCCGCAAGGAGGCGGAGATGGAGCATCGGGCAGTTCGTCCGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5055R-1.in silico     241 AGGACCCGATCCAGGAGTTCCGCAAGGAGGCGGAGATGGAGCATCGGGCAGTTCGTCCGT 300


5055R-1.IR_full       301 TGGCACCGGTTCGCCGGACATCTTTCGCGATCCCACCAACACGGAGGCGCCCACCTGTCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5055R-1.in silico     301 TGGCACCGGTTCGCCGGACATCTTTCGCGATCCCACCAACACGGAGGCGCCCACCTGTCC 360


5055R-1.IR_full       361 GCGGGATCTCTCCACGCCACACATCGAGGTCACCAGCACCACATCGGGACCGATGGCTGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5055R-1.in silico     361 GCGGGATCTCTCCACGCCACACATCGAGGTCACCAGCACCACATCGGGACCGATGGCTGG 420


5055R-1.IR_full       421 ACTCGGAGTTGGACTGATGGTGCGTCGCAGCAGTGATCCCAATCTGCTGGCCTCGCTGAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5055R-1.in silico     421 ACTCGGAGTTGGACTGATGGTGCGTCGCAGCAGTGATCCCAATCTGCTGGCCTCGCTGAA 480


5055R-1.IR_full       481 GGCAGAGGGCAGTAACAAACGC 502
                          ||||||||||||||||||||||
5055R-1.in silico     481 GGCAGAGGGCAGTAACAAACGC 502

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_078659.3  CG5055-RA, transcript variant A (baz), mRNA 
75.93   366  NM_001042818.1  CG5055-RB, transcript variant B (baz), mRNA 
0   NM_080152.2  CG5092-RA (Tor), mRNA 
0   NM_080104.2  CG4032-RA (Abl), mRNA 
0   NM_136591.3  CG8213-RA (CG8213), mRNA 
0   NM_132909.1  CG9782-RA (CG9782), mRNA 
0   NM_138171.1  CG17142-RA, transcript variant A (pyx), mRNA 
0   NM_167813.1  CG17142-RB, transcript variant B (pyx), mRNA 
0   NM_136355.1  CG14593-RA (CG14593), mRNA 
0   NM_132922.2  CG4653-RA (CG4653), mRNA 
0   NM_140156.1  CG6418-RB (CG6418), mRNA 
0   NM_137323.3  CG8910-RA (CG8910), mRNA 
0   NM_137625.1  CG16868-RA (CG16868), mRNA 
0   NM_132440.2  CG2145-RA (CG2145), mRNA 
0   NM_080141.2  CG10120-RB, transcript variant B (Men), mRNA 
0   NM_135877.2  CG4701-RA (CG4701), mRNA 
0   NM_169479.1  CG10120-RA, transcript variant A (Men), mRNA 
0   NM_166019.1  CG18076-RH, transcript variant H (shot), mRNA 
0   NM_141521.1  CG11671-RA (CG11671), mRNA 
0   NM_136994.2  CG13326-RA (CG13326), mRNA 
0   NM_166968.1  CG32498-RN, transcript variant N (dnc), mRNA 
0   NM_168277.1  CG6673-RA, transcript variant A (CG6673), mRNA 
0   NM_132412.1  CG9817-RA (CG9817), mRNA 
0   NM_166964.1  CG32498-RD, transcript variant D (dnc), mRNA 
0   NM_166963.1  CG32498-RK, transcript variant K (dnc), mRNA 
0   NM_166962.1  CG32498-RC, transcript variant C (dnc), mRNA 
0   NM_166965.1  CG32498-RJ, transcript variant J (dnc), mRNA 
0   NM_166961.1  CG32498-RI, transcript variant I (dnc), mRNA 
0   NM_001014504.1  CG30372-RB (CG30372), mRNA 
0   NM_001038935.2  CG33989-RF, transcript variant F (pHCl), mRNA 

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