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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

5013R-3

Symbol

CG5013

Full Name

CG5013

CG No

CG5013

Old CG No

CG5013

Synonyms

CG5013

Accession No (Link to NCBI)

NM_142263.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Park J, Lee J, Kim JH, Lee J, Park H, Lim C.<br> ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.<br> Nucleic Acids Res (2021) 49(19) 11294-11311 [ PubMed ID = 34551427 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCGAGGACAATGACATTGTG

Primer Seq. 3'

aatctagaggtaccCGCTCCAGCAGAAAAGCAACT

Predicted Fragment Size

IR fragment full Seq

0001 AGCGAGGACA ATGACATTGT GNCGNCCACA GcCACTGCCG AGCATATCCG CAAGTTTGTC 
0061 TTCAGCGGAT CACCCGCCGA GCGACTGGAG ATCAAAATAC CTGAGCTGCT TCAGGGAGCC 
0121 TACTCCTTCT ACACATGGCC TTGCGCACCA ATTTTAGCCC ACTTCCTGTG GGAGCGCCGA 
0181 CAGACACTGG CGGGCAAGCG CATCCTGGAG CTGGGAAGCG GCACCGCCCT GCCCGGCATT 
0241 NCTGGCGGCC AAATGCCGCG CCCAAGTGGT ACTTACTGAC AACTgCATTC TGCCCAAGTC 
0301 ACTGGCCCAT ATCCGAAAGT CCTGCCTGGC CAATCAACTG CAGCCGGGCG TTGATATCGA 
0361 TGTGGTGGGC CTGAGTTGGG GCCTGCTGCT GAACAGCGTG TTCCNACTGC CGcCTCTGGA 
0421 CCTTATCATT GCAGCAGACT GCTTCTACGA TCCCAGCGTG TTCGAGGATA TCGTGGTCAC 
0481 AGTTGCTTTT CTGCTGGAGC G

in silico PCR Fragment

0001 AGCGAGGACA ATGACATTGT GGCGGCCACA GCCACTGCCG AGCATATCCG CAAGTTTGTC 
0061 TTCAGCGGAT CACCCGCCGA GCGACTGGAG ATCAAAATAC CTGAGCTGCT TCAGGGTGCC 
0121 TACTCCTTCT ACACATGGCC TTGCGCACCA ATTTTAGCCC ACTTCCTGTG GGAGCGCCGA 
0181 CAGACACTGG CGGGCAAGCG CATCCTGGAG CTGGGAAGCG GCACCGCCCT GCCCGGCATT 
0241 -CTGGCGGCC AAATGCCGCG CCCAAGTGGT ACTTACTGAC AACTGCATTC TGCCCAAGTC 
0301 ACTGGCCCAT ATCCGAAAGT CCTGCCTGGC CAATCAACTG CAGCCGGGCG TTGATATCGA 
0361 TGTGGTGGGC CTGAGTTGGG GCCTGCTGCT GAACAGCGTG TTCCGACTGC CGCCTCTGGA 
0421 CCTTATCATT GCAGCAGACT GCTTCTACGA TCCCAGCGTG TTCGAGGATA TCGTGGTCAC 
0481 AGTTGCTTTT CTGCTGGAGC G

Assemble Data

<PRE>

5013R-3.IR_full       1   AGCGAGGACAATGACATTGTG<span class="snp"><tt>N</tt></span>CG<span class="snp"><tt>N</tt></span>CCACAGCCACTGCCGAGCATATCCGCAAGTTTGTC 60
                          |||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||
5013R-3.in silico     1   AGCGAGGACAATGACATTGTG<span class="snp"><tt>G</tt></span>CG<span class="snp"><tt>G</tt></span>CCACAGCCACTGCCGAGCATATCCGCAAGTTTGTC 60


5013R-3.IR_full       61  TTCAGCGGATCACCCGCCGAGCGACTGGAGATCAAAATACCTGAGCTGCTTCAGGG<span class="snp"><tt>A</tt></span>GCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
5013R-3.in silico     61  TTCAGCGGATCACCCGCCGAGCGACTGGAGATCAAAATACCTGAGCTGCTTCAGGG<span class="snp"><tt>T</tt></span>GCC 120


5013R-3.IR_full       121 TACTCCTTCTACACATGGCCTTGCGCACCAATTTTAGCCCACTTCCTGTGGGAGCGCCGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5013R-3.in silico     121 TACTCCTTCTACACATGGCCTTGCGCACCAATTTTAGCCCACTTCCTGTGGGAGCGCCGA 180


5013R-3.IR_full       181 CAGACACTGGCGGGCAAGCGCATCCTGGAGCTGGGAAGCGGCACCGCCCTGCCCGGCATT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5013R-3.in silico     181 CAGACACTGGCGGGCAAGCGCATCCTGGAGCTGGGAAGCGGCACCGCCCTGCCCGGCATT 240


5013R-3.IR_full        241 CTGGCGGCCAAATGCCGCGCCCAAGTGGTACTTACTGACAACTGCATTCTGCCCAAGTC 299
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5013R-3.in silico      241 CTGGCGGCCAAATGCCGCGCCCAAGTGGTACTTACTGACAACTGCATTCTGCCCAAGTC 299


5013R-3.IR_full       301 ACTGGCCCATATCCGAAAGTCCTGCCTGGCCAATCAACTGCAGCCGGGCGTTGATATCGA 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5013R-3.in silico     301 ACTGGCCCATATCCGAAAGTCCTGCCTGGCCAATCAACTGCAGCCGGGCGTTGATATCGA 359


5013R-3.IR_full       361 TGTGGTGGGCCTGAGTTGGGGCCTGCTGCTGAACAGCGTGTTCC<span class="snp"><tt>N</tt></span>ACTGCCGCCTCTGGA 419
                          ||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||
5013R-3.in silico     361 TGTGGTGGGCCTGAGTTGGGGCCTGCTGCTGAACAGCGTGTTCC<span class="snp"><tt>G</tt></span>ACTGCCGCCTCTGGA 419


5013R-3.IR_full       421 CCTTATCATTGCAGCAGACTGCTTCTACGATCCCAGCGTGTTCGAGGATATCGTGGTCAC 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5013R-3.in silico     421 CCTTATCATTGCAGCAGACTGCTTCTACGATCCCAGCGTGTTCGAGGATATCGTGGTCAC 479


5013R-3.IR_full       481 AGTTGCTTTTCTGCTGGAGCG 500
                          |||||||||||||||||||||
5013R-3.in silico     481 AGTTGCTTTTCTGCTGGAGCG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_142263.2	CG5013-RA (CG5013), mRNA
0	0	3	2	3	NM_206278.1	CG5406-RC, transcript variant C (sif), mRNA
0	0	3	2	3	NM_079908.2	CG5406-RA, transcript variant A (sif), mRNA
0	0	3	2	2	NM_168126.1	CG5406-RB, transcript variant B (sif), mRNA
0	0	2	3	5	NM_136731.1	CG12908-RA, transcript variant A (Ndg), mRNA
0	0	2	3	5	NM_206074.1	CG12908-RB, transcript variant B (Ndg), mRNA
0	0	2	3	4	NM_166326.1	CG15118-RC, transcript variant C (CG15118), mRNA
0	0	2	3	4	NM_166325.1	CG15118-RA, transcript variant A (CG15118), mRNA
0	0	2	3	4	NM_137552.2	CG15118-RB, transcript variant B (CG15118), mRNA
0	0	2	3	4	NM_166327.1	CG15118-RD, transcript variant D (CG15118), mRNA
0	0	2	2	3	NM_164597.1	CG15626-RB, transcript variant B (CG15626), mRNA
0	0	2	2	3	NM_135020.2	CG15626-RA, transcript variant A (CG15626), mRNA
0	0	1	4	4	NM_143372.1	CG9995-RA (htt), mRNA
0	0	1	3	8	NM_143018.2	CG5794-RD, transcript variant D (CG5794), mRNA
0	0	1	3	6	NM_206564.1	CG5794-RE, transcript variant E (CG5794), mRNA
0	0	1	3	4	NM_167216.1	CG15312-RC, transcript variant C (CG15312), mRNA
0	0	1	3	4	NM_132373.2	CG15312-RA, transcript variant A (CG15312), mRNA
0	0	1	3	4	NM_167215.1	CG15312-RB, transcript variant B (CG15312), mRNA
0	0	1	3	4	NM_170158.1	CG5794-RB, transcript variant B (CG5794), mRNA
0	0	1	3	2	NM_079771.1	CG11921-RA (fd96Ca), mRNA
0	0	1	3	2	NM_137573.2	CG10073-RA (CG10073), mRNA
0	0	1	2	6	NM_136538.2	CG30359-RA (CG30359), mRNA
0	0	1	2	5	NM_001038987.1	CG1721-RB, transcript variant B (Pglym78), mRNA
0	0	1	2	5	NM_079822.2	CG1721-RA, transcript variant A (Pglym78), mRNA
0	0	1	2	5	NM_001038986.1	CG1721-RC, transcript variant C (Pglym78), mRNA
0	0	1	2	3	NM_079946.3	CG6657-RB, transcript variant B (veg), mRNA
0	0	1	2	3	NM_166202.2	CG6657-RA, transcript variant A (veg), mRNA
0	0	1	2	3	NM_079821.2	CG1954-RA (Pkc98E), mRNA
0	0	1	2	2	NM_079998.2	CG5912-RA (arr), mRNA
0	0	1	2	2	NM_132960.2	CG4949-RA, transcript variant A (CG4949), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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