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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5000R-3 |
| Symbol |
msps |
Full Name |
mini spindles |
| CG No |
CG5000 |
Old CG No |
CG5000 |
| Synonyms |
TOG, XMAP215, Dis1, Msps, CG5000, anon-WO0140519.136, msps |
| Accession No (Link to NCBI) |
NM_169698.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tang Q, Rui M, Bu S, Wang Y, Chew LY, Yu F.<br> A microtubule polymerase is required for microtubule orientation and dendrite pruning in Drosophila.<br> EMBO J (2020) 39(10) e103549
[
PubMed ID = 32267553
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGGCCTTGTTTATTGCCCGTG |
| Primer Seq. 3' |
aatctagaggtaccGGGGGTTATTTCGCGTTCCGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGGCCTTGTT TATTGCCCGT GCCCTAACCC GCACCCAACC AACCGCGCTA AACAAAAAGC
0061 TGCTTAAACT TCTGACCACC AGTCTAGTGA AAACGCTTAA CGAACCAGAT CCAACCGTAC
0121 GCGACAGCAG NCGCCGNAAG CTCTTGGTAC TTTAATAAAG CTAATGGGAG ACAAAGCGGT
0181 GACTCCTTTG CTCGCCGACG TCGATCCACT CAAAATGGCC AAGATAAAGG AGTGCCAAGA
0241 AAAGGCGGAG ATTAAAATAA AGGTTGCAGG TCCAAAGAAG GAGACACGAC CTGCATCTGC
0301 ACCGACTGCG AAAGCTGCNT GCTCCGGCTA AGACAGTGGC TGGTAGTGTG GACCCTAAGC
0361 CAGTAACCCG ACCGGCCACT ACAGGGGCAC GTAAGGTCTT GAAAAAACCG GCGACGGTTA
0421 GTGGAGGAGN NGCTACATCT GCCCCAACTG CAGCTCTTAA AGCGGGTGGA AAACCCCTAG
0481 CCACGGAACG CGAAATAACC CCC |
| in silico PCR Fragment |
0001 CGGCCTTGTT TATTGCCCGT GCCCTAACCC GCACCCAACC AACCGCGCTA AACAAAAAGC
0061 TGCTTAAACT TCTGACCACC AGTCTAGTGA AAACGCTTAA CGAACCAGAT CCAACCGTAC
0121 GCGACAGCAG -CGCCG-AAG CTCTTGGTAC TTTAATAAAG CTAATGGGAG ACAAAGCGGT
0181 GACTCCTTTG CTCGCCGACG TCGATCCACT CAAAATGGCC AAGATAAAGG AGTGCCAAGA
0241 AAAGGCGGAG ATTAAAATAA AGGTTGCAGG TCCAAAGAAG GAGACACGAC CTGCATCTGC
0301 ACCGACTGCG AAAGCTGC-T GCTCCGGCTA AGACAGTGGC TGGTAGTGTG GACCCTAAGC
0361 CAGTAACCCG ACCGGCCACT ACAGGGGCAC GTAAGGTCTT GAAAAAACCG GCGACGGTTA
0421 GTGGAGGAGG AGCTACATCT GCCCCAACTG CAGCTCTTAA AGCGGGTGGA AAACCCCTAG
0481 CCACGGAACG CGAAATAACC CCC |
| Assemble Data |
<PRE>
5000R-3.IR_full 1 CGGCCTTGTTTATTGCCCGTGCCCTAACCCGCACCCAACCAACCGCGCTAAACAAAAAGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5000R-3.in silico 1 CGGCCTTGTTTATTGCCCGTGCCCTAACCCGCACCCAACCAACCGCGCTAAACAAAAAGC 60
5000R-3.IR_full 61 TGCTTAAACTTCTGACCACCAGTCTAGTGAAAACGCTTAACGAACCAGATCCAACCGTAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5000R-3.in silico 61 TGCTTAAACTTCTGACCACCAGTCTAGTGAAAACGCTTAACGAACCAGATCCAACCGTAC 120
5000R-3.IR_full 121 GCGACAGCAG<span class="snp"><tt>N</tt></span>CGCCG<span class="snp"><tt>N</tt></span>AAGCTCTTGGTACTTTAATAAAGCTAATGGGAGACAAAGCGGT 180
||||||||||<span class="snp"><tt> </tt></span>|||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||
5000R-3.in silico 121 GCGACAGCAG<span class="snp"><tt>-</tt></span>CGCCG<span class="snp"><tt>-</tt></span>AAGCTCTTGGTACTTTAATAAAGCTAATGGGAGACAAAGCGGT 180
5000R-3.IR_full 181 GACTCCTTTGCTCGCCGACGTCGATCCACTCAAAATGGCCAAGATAAAGGAGTGCCAAGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5000R-3.in silico 181 GACTCCTTTGCTCGCCGACGTCGATCCACTCAAAATGGCCAAGATAAAGGAGTGCCAAGA 240
5000R-3.IR_full 241 AAAGGCGGAGATTAAAATAAAGGTTGCAGGTCCAAAGAAGGAGACACGACCTGCATCTGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5000R-3.in silico 241 AAAGGCGGAGATTAAAATAAAGGTTGCAGGTCCAAAGAAGGAGACACGACCTGCATCTGC 300
5000R-3.IR_full 301 ACCGACTGCGAAAGCTGC<span class="snp"><tt>N</tt></span>TGCTCCGGCTAAGACAGTGGCTGGTAGTGTGGACCCTAAGC 360
||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||
5000R-3.in silico 301 ACCGACTGCGAAAGCTGC<span class="snp"><tt>-</tt></span>TGCTCCGGCTAAGACAGTGGCTGGTAGTGTGGACCCTAAGC 360
5000R-3.IR_full 361 CAGTAACCCGACCGGCCACTACAGGGGCACGTAAGGTCTTGAAAAAACCGGCGACGGTTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5000R-3.in silico 361 CAGTAACCCGACCGGCCACTACAGGGGCACGTAAGGTCTTGAAAAAACCGGCGACGGTTA 420
5000R-3.IR_full 421 GTGGAGGAG<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>GCTACATCTGCCCCAACTGCAGCTCTTAAAGCGGGTGGAAAACCCCTAG 480
|||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
5000R-3.in silico 421 GTGGAGGAG<span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span>GCTACATCTGCCCCAACTGCAGCTCTTAAAGCGGGTGGAAAACCCCTAG 480
5000R-3.IR_full 481 CCACGGAACGCGAAATAACCCCC 503
|||||||||||||||||||||||
5000R-3.in silico 481 CCACGGAACGCGAAATAACCCCC 503
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_169698.1 |
CG5000-RA (msps), mRNA |
| 0 |
0 |
5 |
6 |
6 |
NM_137050.1 |
CG6209-RA (CG6209), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_132084.2 |
CG12219-RA (CG12219), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168171.1 |
CG32397-RA (CG32397), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_134496.2 |
CG14215-RA (CG14215), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001015347.1 |
CG40084-PD.3 (CG40084), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001015346.1 |
CG40084-PC.3 (CG40084), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001015104.1 |
CG41048-PA.3 (CG41048), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001031999.1 |
CG17117-RF, transcript variant F (hth), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137081.2 |
CG30483-RA (Prosap), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_079075.4 |
CG10036-RA, transcript variant A (otp), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_001043098.1 |
CG10036-RC, transcript variant C (otp), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_206187.2 |
CG10036-RB, transcript variant B (otp), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079997.2 |
CG13580-RA (Crtp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001032232.1 |
CG11919-RA, transcript variant A (CG11919), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001032230.1 |
CG18003-RB, transcript variant B (CG18003), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_133030.2 |
CG6842-RA (CG6842), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001032231.1 |
CG18003-RA, transcript variant A (CG18003), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137517.4 |
CG18604-RA (CG18604), mRNA |
| 0 |
0 |
0 |
7 |
6 |
NM_132212.2 |
CG1575-RA (CG1575), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_137856.4 |
CG13510-RA (CG13510), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_080092.2 |
CG13207-RB, transcript variant B (nompA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_206096.1 |
CG13207-RD, transcript variant D (nompA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_165821.2 |
CG13207-RC, transcript variant C (nompA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_165822.2 |
CG13207-RA, transcript variant A (nompA), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_079902.2 |
CG6741-RB, transcript variant B (a), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_166514.1 |
CG6741-RA, transcript variant A (a), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_001014543.1 |
CG6741-RC, transcript variant C (a), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_080516.1 |
CG16757-RA (Spn), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_080142.2 |
CG1925-RA (mus205), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| + |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| + |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
