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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4916R-2 |
| Symbol |
me31B |
Full Name |
maternal expression at 31B |
| CG No |
CG4916 |
Old CG No |
CG4916 |
| Synonyms |
Me31B, CG4916, ME31B, DmRH3, l(2)k06607, me31B, Me31b, Dhh1p/Me31b |
| Accession No (Link to NCBI) |
NM_078809.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Lee J, Yoo E, Lee H, Park K, Hur JH, Lim C.<br> LSM12 and ME31B/DDX6 Define Distinct Modes of Posttranscriptional Regulation by ATAXIN-2 Protein Complex in Drosophila Circadian Pacemaker Neurons.<br> Mol Cell (2017) 66(1) 129-140.e7
[
PubMed ID = 28388438
]
[
RRC reference
]
Barbee SA, Estes PS, Cziko AM, Hillebrand J, Luedeman RA, Coller JM, Johnson N, Howlett IC, Geng C, Ueda R, Brand AH, Newbury SF, Wilhelm JE, Levine RB, Nakamura A, Parker R, Ramaswami M.<br> Staufen- and FMRP-containing neuronal RNPs are structurally and functionally related to somatic P bodies.<br> Neuron (2006) 52(6) 997-1009
[
PubMed ID = 17178403
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTATTGCCACTCCCGGACGAA |
| Primer Seq. 3' |
aatctagaggtaccACAGAGTCCTTGGCGGAAATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTATTGCCAC TCCCGGACGA ATATTAGATC TGATGGATAA GAAAGTTGCC GATATGTCGC
0061 ATTGCAGAAT ATTAGTTTTG GATGAGGCCG ATAAGCTATT GTCGCTTGAT TTTCAAGGCA
0121 TGCTGGATCA TGTTATACTA AAGTTACCAA AGGATCCACA GATCTTGCTA TTTTCCGCAA
0181 CATTTCCACT AACAGTCAAG AATTTCATGG AGAAGCATTT ACGCGAGCCT TACGAGATCA
0241 ACCTGATGGA GGAGCTAACA CTGAAGGGTG TCACTCAGTA CTATGCATTC GTACAGGAGC
0301 GCCAGAAAGT ACACTGCTTG AATACGCTCT TTTCGAAGCT GCAGATCAAT CAGTCTATCA
0361 TATTCTGCAA TTCCACGCAA CGTGTCGAGC TATTGGCCAA AAAGATCACA GAGCTTGGAT
0421 ATTGCTGTTA CTATATACAC GCNTAAGATG GCCCAGGCAC ACAGAAATCG AGTATTCCAC
0481 GATTTCCGCC AAGGACTCTG T |
| in silico PCR Fragment |
0001 TTATTGCCAC TCCCGGACGA ATATTAGATC TGATGGATAA GAAAGTTGCC GATATGTCGC
0061 ATTGCAGAAT ATTAGTTTTG GATGAGGCCG ATAAGCTATT GTCGCTTGAT TTTCAAGGCA
0121 TGCTGGATCA TGTTATACTA AAGTTACCAA AGGATCCACA GATCTTGCTA TTTTCCGCAA
0181 CATTTCCACT AACAGTCAAG AATTTCATGG AGAAGCATTT ACGCGAGCCT TACGAGATCA
0241 ACCTGATGGA GGAGCTAACA CTGAAGGGTG TCACTCAGTA CTATGCATTC GTACAGGAGC
0301 GCCAGAAAGT ACACTGCTTG AATACGCTCT TTTCGAAGCT GCAGATCAAT CAGTCTATCA
0361 TATTCTGCAA TTCCACGCAA CGTGTCGAGC TATTGGCCAA AAAGATCACA GAGCTTGGAT
0421 ATTGCTGTTA CTATATACAC GC-TAAGATG GCCCAGGCAC ACAGAAATCG AGTATTCCAC
0481 GATTTCCGCC AAGGACTCTG T |
| Assemble Data |
<PRE>
4916R-2.IR_full 1 TTATTGCCACTCCCGGACGAATATTAGATCTGATGGATAAGAAAGTTGCCGATATGTCGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4916R-2.in silico 1 TTATTGCCACTCCCGGACGAATATTAGATCTGATGGATAAGAAAGTTGCCGATATGTCGC 60
4916R-2.IR_full 61 ATTGCAGAATATTAGTTTTGGATGAGGCCGATAAGCTATTGTCGCTTGATTTTCAAGGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4916R-2.in silico 61 ATTGCAGAATATTAGTTTTGGATGAGGCCGATAAGCTATTGTCGCTTGATTTTCAAGGCA 120
4916R-2.IR_full 121 TGCTGGATCATGTTATACTAAAGTTACCAAAGGATCCACAGATCTTGCTATTTTCCGCAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4916R-2.in silico 121 TGCTGGATCATGTTATACTAAAGTTACCAAAGGATCCACAGATCTTGCTATTTTCCGCAA 180
4916R-2.IR_full 181 CATTTCCACTAACAGTCAAGAATTTCATGGAGAAGCATTTACGCGAGCCTTACGAGATCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4916R-2.in silico 181 CATTTCCACTAACAGTCAAGAATTTCATGGAGAAGCATTTACGCGAGCCTTACGAGATCA 240
4916R-2.IR_full 241 ACCTGATGGAGGAGCTAACACTGAAGGGTGTCACTCAGTACTATGCATTCGTACAGGAGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4916R-2.in silico 241 ACCTGATGGAGGAGCTAACACTGAAGGGTGTCACTCAGTACTATGCATTCGTACAGGAGC 300
4916R-2.IR_full 301 GCCAGAAAGTACACTGCTTGAATACGCTCTTTTCGAAGCTGCAGATCAATCAGTCTATCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4916R-2.in silico 301 GCCAGAAAGTACACTGCTTGAATACGCTCTTTTCGAAGCTGCAGATCAATCAGTCTATCA 360
4916R-2.IR_full 361 TATTCTGCAATTCCACGCAACGTGTCGAGCTATTGGCCAAAAAGATCACAGAGCTTGGAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4916R-2.in silico 361 TATTCTGCAATTCCACGCAACGTGTCGAGCTATTGGCCAAAAAGATCACAGAGCTTGGAT 420
4916R-2.IR_full 421 ATTGCTGTTACTATATACACGC<span class="snp"><tt>N</tt></span>TAAGATGGCCCAGGCACACAGAAATCGAGTATTCCAC 480
||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||
4916R-2.in silico 421 ATTGCTGTTACTATATACACGC<span class="snp"><tt>-</tt></span>TAAGATGGCCCAGGCACACAGAAATCGAGTATTCCAC 480
4916R-2.IR_full 481 GATTTCCGCCAAGGACTCTGT 501
|||||||||||||||||||||
4916R-2.in silico 481 GATTTCCGCCAAGGACTCTGT 501
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_078809.2 |
CG4916-RA, transcript variant A (me31B), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_164898.1 |
CG4916-RB, transcript variant B (me31B), mRNA |
| 0 |
0 |
2 |
6 |
8 |
NM_079817.2 |
CG1842-RA (Dhc98D), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_135011.2 |
CG15631-RA (CG15631), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001038795.1 |
CG8683-RA (CG8683), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_206610.1 |
CG14047-RB, transcript variant B (CG14047), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_130645.2 |
CG14047-RA, transcript variant A (CG14047), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_057796.2 |
CG10379-RA (mbc), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165492.1 |
CG3287-RC, transcript variant C (CG3287), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165491.1 |
CG3287-RB, transcript variant B (CG3287), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136443.2 |
CG1620-RA (CG1620), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078773.2 |
CG31628-RA, transcript variant A (ade3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165928.1 |
CG8811-RB, transcript variant B (muskelin), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136957.2 |
CG8811-RA, transcript variant A (muskelin), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057393.3 |
CG3158-RA (spn-E), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057679.4 |
CG5371-RA (RnrL), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_057258.2 |
CG10699-RA, transcript variant A (Lim3), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_165277.1 |
CG10699-RB, transcript variant B (Lim3), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_132370.1 |
CG2989-RA (CG2989), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_080167.3 |
CG9074-RA (Mst57Da), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001014725.1 |
CG4027-RD, transcript variant D (Act5C), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001014726.1 |
CG4027-RC, transcript variant C (Act5C), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_176735.1 |
CG33206-RB, transcript variant B (l(1)G0168), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_176734.1 |
CG33206-RA, transcript variant A (l(1)G0168), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_165342.3 |
CG9326-RB, transcript variant B (CG9326), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_206011.2 |
CG9326-RD, transcript variant D (CG9326), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_143375.1 |
CG14061-RA (CG14061), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_001014620.1 |
CG9918-RD (CG9918), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_166177.2 |
CG15925-RA (CG15925), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
dme03018
:
RNA degradation
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