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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4909R-1 |
| Symbol |
POSH |
Full Name |
Plenty of SH3s |
| CG No |
CG4909 |
Old CG No |
CG4909 |
| Synonyms |
155116_at, DPOSH, posh, CG4909, l(2)k15815, GM09451, BEST:GM09451, POSH, dPOSH |
| Accession No (Link to NCBI) |
NM_079052.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nelson B, Freisinger T, Ishii K, Okado K, Shinzawa N, Fukumoto S, Kanuka H.
Activation of Imd pathway in hemocyte confers infection resistance through humoral response in Drosophila.
Biochem Biophys Res Commun (2013) 430(3) 1120-5
[
PubMed ID = 23261474
]
[
RRC reference
]
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCACGTTAAACGACCTGTTGGA |
| Primer Seq. 3' |
aatctagaggtaccTGTTGTCGATGCGATGCTTGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CACGTTAAAC GACCTGTTGG AGTGCTCCGT GTGTCTTGAG CGACTGGACA CCACATCGAA
0061 GGTGCTGCCA TGCCAGCACA CCTTCTGCCG CAAATGCTTG CAGGACATTG TGGCCAGTCA
0121 GCACAAGTTG CGATGCCCGG AGTGCCGCAT CCTGGTCTCT TGCAAAATTG ATGAGCTGCC
0181 TCCAAACGTC TTGCTGATGC GAATCTTAGA AGGCATGAAA CAAAATGCAG CAGCTGGCAA
0241 AGGAGAAGAA AAGGGAGAGG AGACTGAAAC ACAGCCGGAA AGGTGCTGCC AAACCTCANC
0301 CGCCAGCGGA ATCAGTgGCC CCGCCTGACA ACCAACTACT CCAGCTGCAG TCACATCAGC
0361 AATCTCATCA GCCGGCTCGT CACAAGCAAC GTCGATTTCT ACTCCCCCAC GCCTATGCCC
0421 TCTTTNNCTT CGcCTCCGGT GAAGCCACCG ATCTAAAGTT CAAGAAAGGG GATCTGATAC
0481 TGATCAAGCA TCGCATCGAC AACA |
| in silico PCR Fragment |
0001 CACGTTAAAC GACCTGTTGG AGTGCTCCGT GTGTCTTGAG CGACTGGACA CCACATCGAA
0061 GGTGCTGCCA TGCCAGCACA CCTTCTGCCG CAAATGCTTG CAGGACATTG TGGCCAGTCA
0121 GCACAAGTTG CGATGCCCGG AGTGCCGCAT CCTGGTCTCT TGCAAAATTG ATGAGCTGCC
0181 TCCAAACGTC TTGCTGATGC GAATCTTAGA AGGCATGAAA CAAAATGCAG CAGCTGGCAA
0241 AGGAGAAGAA AAGGGAGAGG AGACTGAAAC ACAGCCGGAA AGG----GCC AAACCTCAGC
0301 CGCCAGCGGA ATCAGTGGCC CCGCCTGACA ACCAACTACT CCAGCTGCAG TCACATCAGC
0361 AATCTCATCA GCCGGCTCGT CACAAGCAAC GTCGATTTCT ACTCCCCCAC GCCTATGCCC
0421 TCTTTGACTT CGCCTCCGGT GAAGCCACCG ATCTAAAGTT CAAGAAAGGG GATCTGATAC
0481 TGATCAAGCA TCGCATCGAC AACA |
| Assemble Data |
4909R-1.IR_full 1 CACGTTAAACGACCTGTTGGAGTGCTCCGTGTGTCTTGAGCGACTGGACACCACATCGAA 60
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4909R-1.in silico 1 CACGTTAAACGACCTGTTGGAGTGCTCCGTGTGTCTTGAGCGACTGGACACCACATCGAA 60
4909R-1.IR_full 61 GGTGCTGCCATGCCAGCACACCTTCTGCCGCAAATGCTTGCAGGACATTGTGGCCAGTCA 120
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4909R-1.in silico 61 GGTGCTGCCATGCCAGCACACCTTCTGCCGCAAATGCTTGCAGGACATTGTGGCCAGTCA 120
4909R-1.IR_full 121 GCACAAGTTGCGATGCCCGGAGTGCCGCATCCTGGTCTCTTGCAAAATTGATGAGCTGCC 180
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4909R-1.in silico 121 GCACAAGTTGCGATGCCCGGAGTGCCGCATCCTGGTCTCTTGCAAAATTGATGAGCTGCC 180
4909R-1.IR_full 181 TCCAAACGTCTTGCTGATGCGAATCTTAGAAGGCATGAAACAAAATGCAGCAGCTGGCAA 240
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4909R-1.in silico 181 TCCAAACGTCTTGCTGATGCGAATCTTAGAAGGCATGAAACAAAATGCAGCAGCTGGCAA 240
4909R-1.IR_full 241 AGGAGAAGAAAAGGGAGAGGAGACTGAAACACAGCCGGAAAGGTGCTGCCAAACCTCANC 300
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4909R-1.in silico 241 AGGAGAAGAAAAGGGAGAGGAGACTGAAACACAGCCGGAAAGG----GCCAAACCTCAGC 300
4909R-1.IR_full 301 CGCCAGCGGAATCAGTGGCCCCGCCTGACAACCAACTACTCCAGCTGCAGTCACATCAGC 360
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4909R-1.in silico 301 CGCCAGCGGAATCAGTGGCCCCGCCTGACAACCAACTACTCCAGCTGCAGTCACATCAGC 360
4909R-1.IR_full 361 AATCTCATCAGCCGGCTCGTCACAAGCAACGTCGATTTCTACTCCCCCACGCCTATGCCC 420
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4909R-1.in silico 361 AATCTCATCAGCCGGCTCGTCACAAGCAACGTCGATTTCTACTCCCCCACGCCTATGCCC 420
4909R-1.IR_full 421 TCTTTNNCTTCGCCTCCGGTGAAGCCACCGATCTAAAGTTCAAGAAAGGGGATCTGATAC 480
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4909R-1.in silico 421 TCTTTGACTTCGCCTCCGGTGAAGCCACCGATCTAAAGTTCAAGAAAGGGGATCTGATAC 480
4909R-1.IR_full 481 TGATCAAGCATCGCATCGACAACA 504
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4909R-1.in silico 481 TGATCAAGCATCGCATCGACAACA 504
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
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| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_079052.2 |
CG4909-RA (POSH), mRNA |
| 0 |
0 |
11 |
4 |
2 |
NM_166318.1 |
CG15105-RB, transcript variant B (CG15105), mRNA |
| 0 |
0 |
11 |
4 |
2 |
NM_137546.2 |
CG15105-RA, transcript variant A (CG15105), mRNA |
| 0 |
0 |
3 |
8 |
7 |
NM_136637.1 |
CG13954-RA (CG13954), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_140155.2 |
CG7958-RA, transcript variant A (tna), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_168422.1 |
CG7958-RB, transcript variant B (tna), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_140424.1 |
CG9007-RA (CG9007), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_133156.1 |
CG12202-RA (Nat1), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_167202.2 |
CG1354-RD, transcript variant D (CG1354), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_167201.2 |
CG1354-RC, transcript variant C (CG1354), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_132352.2 |
CG1354-RA, transcript variant A (CG1354), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_167203.1 |
CG1354-RB, transcript variant B (CG1354), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_139523.1 |
CG12734-RA, transcript variant A (CG12734), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206262.1 |
CG12734-RB, transcript variant B (CG12734), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_169061.1 |
CG12734-RB, transcript variant B (CG12734), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_169060.1 |
CG12734-RB, transcript variant B (CG12734), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079088.2 |
CG9952-RA (ppa), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139917.2 |
CG7927-RA (CG7927), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141807.1 |
CG5214-RA (CG5214), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079252.2 |
CG5962-RA (Arr2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167266.1 |
CG12101-RB, transcript variant B (Hsp60), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078560.2 |
CG12101-RA, transcript variant A (Hsp60), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136154.1 |
CG10631-RA (CG10631), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079202.2 |
CG7452-RA (Syx17), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_058067.2 |
CG6147-RA (Tsc1), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_132908.2 |
CG4453-RA (Nup153), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_135923.2 |
CG18801-RA (Ku80), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_057338.2 |
CG8246-RA (Poxn), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206598.2 |
CG4122-RE, transcript variant E (svr), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206599.1 |
CG4122-RD, transcript variant D (svr), mRNA |
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