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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4887R-3

Symbol

CG4887

Full Name

CG4887

CG No

CG4887

Old CG No

CG4887

Synonyms

cg4887, CG4887

Accession No (Link to NCBI)

NM_134738.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Park J, Lee J, Kim JH, Lee J, Park H, Lim C.<br> ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.<br> Nucleic Acids Res (2021) 49(19) 11294-11311 [ PubMed ID = 34551427 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACTCGAGTAGTCGGCGCAGTT

Primer Seq. 3'

aatctagaggtaccTTCCCTGCTCCGACTTCTGTT

Predicted Fragment Size

IR fragment full Seq

0001 ACTCGAGTAG TCGGCGCAGT TTTTCTGGTG GAAGTGAAAA CGAGGGTGGA CGGGGCAGCG 
0061 ACTACTATAG ATCCAGGCCG GGGTCTCGAT ACAGTCGATC CAGGTCGCGT TCCCGTGANG 
0121 CGCAACCGCA GCCATGGAGG AATCAGGCAC CGGAACTCTC GCTCTCGAAG TCGGGATCGN 
0181 GGATCGGTCG CCCGTGTTCA GAAACGATCA GCATCGCGGT GGTCNGAGGA GGAGCTTGGT 
0241 AACGGGGACC CCGNACCTTT ACCACAGCCT GATCAACGAC GACTACAGGG ATCAGGATGA 
0301 GCGCAACTAC AACTCCAGGA GCAACTTTGA GAATCGTCAG TTCCGGCGAC ATGATAGCTT 
0361 CGACCGTCGG CATCGGGACC GAGATGGCGA GAGCGATCGG GAGCTAAACG ACTACGAGTA 
0421 CGAacANCGC TGCCCGCGAC TTGGACTCAC GTGATCGTAG CTCCACGGAC AGGGACTGGT 
0481 ATCACAACAG AAGTCGGAGC AGGGAA

in silico PCR Fragment

0001 ACTCGAGTAG TCGGCGCAGT TTTTCTGGTG GAAGTGAAAA CGAGGGTGGA CGGGGCAGCG 
0061 ACTACTATAG ATCCAGGCCG GGGTCTCGAT ACAGTCGATC CAGGTCGCGT TCCCGTGA-G 
0121 CGCAACCGCA GCCATGGAGG AATCAGGCAC CGGAACTCTC GCTCTCGAAG TCGGGATCG- 
0181 GGATCGGTCG CCCGTGTTCA GAAACGATCA GCATCGCGGT GGTC-GAGGA GGAGC-TGGT 
0241 AACGGGGACC CCG-ACCTTT ACCACAGCCT GATCAACGAC GACTACAGGG ATCAGGATGA 
0301 GCGCAACTAC AACTCCAGGA GCAACTTTGA GAATCGTCAG TTCCGGCGAC ATGATAGCTT 
0361 CGACCGTCGG CATCGGGACC GAGATGGCGA GAGCGATCGG GAGCTAAACG ACTACGAGTA 
0421 CGAACAGCGC T-CCCGCGAC TTGGACTCAC GTGATCGTAG CTCCACGGAC AGGGACTGGT 
0481 ATCACAACAG AAGTCGGAGC AGGGAA

Assemble Data

<PRE>

4887R-3.IR_full       1   ACTCGAGTAGTCGGCGCAGTTTTTCTGGTGGAAGTGAAAACGAGGGTGGACGGGGCAGCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4887R-3.in silico     1   ACTCGAGTAGTCGGCGCAGTTTTTCTGGTGGAAGTGAAAACGAGGGTGGACGGGGCAGCG 60


4887R-3.IR_full       61  ACTACTATAGATCCAGGCCGGGGTCTCGATACAGTCGATCCAGGTCGCGTTCCCGTGA<span class="snp"><tt>N</tt></span>G 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
4887R-3.in silico     61  ACTACTATAGATCCAGGCCGGGGTCTCGATACAGTCGATCCAGGTCGCGTTCCCGTGA<span class="snp"><tt>-</tt></span>G 120


4887R-3.IR_full       121 CGCAACCGCAGCCATGGAGGAATCAGGCACCGGAACTCTCGCTCTCGAAGTCGGGATCG<span class="snp"><tt>N</tt></span> 180
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
4887R-3.in silico     121 CGCAACCGCAGCCATGGAGGAATCAGGCACCGGAACTCTCGCTCTCGAAGTCGGGATCG<span class="snp"><tt>-</tt></span> 180


4887R-3.IR_full       181 GGATCGGTCGCCCGTGTTCAGAAACGATCAGCATCGCGGTGGTC<span class="snp"><tt>N</tt></span>GAGGAGGAGC<span class="snp"><tt>T</tt></span>TGGT 240
                          ||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
4887R-3.in silico     181 GGATCGGTCGCCCGTGTTCAGAAACGATCAGCATCGCGGTGGTC<span class="snp"><tt>-</tt></span>GAGGAGGAGC<span class="snp"><tt>-</tt></span>TGGT 240


4887R-3.IR_full       241 AACGGGGACCCCG<span class="snp"><tt>N</tt></span>ACCTTTACCACAGCCTGATCAACGACGACTACAGGGATCAGGATGA 300
                          |||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||
4887R-3.in silico     241 AACGGGGACCCCG<span class="snp"><tt>-</tt></span>ACCTTTACCACAGCCTGATCAACGACGACTACAGGGATCAGGATGA 300


4887R-3.IR_full       301 GCGCAACTACAACTCCAGGAGCAACTTTGAGAATCGTCAGTTCCGGCGACATGATAGCTT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4887R-3.in silico     301 GCGCAACTACAACTCCAGGAGCAACTTTGAGAATCGTCAGTTCCGGCGACATGATAGCTT 360


4887R-3.IR_full       361 CGACCGTCGGCATCGGGACCGAGATGGCGAGAGCGATCGGGAGCTAAACGACTACGAGTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4887R-3.in silico     361 CGACCGTCGGCATCGGGACCGAGATGGCGAGAGCGATCGGGAGCTAAACGACTACGAGTA 420


4887R-3.IR_full       421 CGAACA<span class="snp"><tt>N</tt></span>CGCT<span class="snp"><tt>G</tt></span>CCCGCGACTTGGACTCACGTGATCGTAGCTCCACGGACAGGGACTGGT 480
                          ||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
4887R-3.in silico     421 CGAACA<span class="snp"><tt>G</tt></span>CGCT<span class="snp"><tt>-</tt></span>CCCGCGACTTGGACTCACGTGATCGTAGCTCCACGGACAGGGACTGGT 480


4887R-3.IR_full       481 ATCACAACAGAAGTCGGAGCAGGGAA 506
                          ||||||||||||||||||||||||||
4887R-3.in silico     481 ATCACAACAGAAGTCGGAGCAGGGAA 506

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	5	NM_134738.2	CG4887-RA (CG4887), mRNA
0	0	4	2	2	NR_001305.1	CG4887-RA (CG4887), mRNA, mRNA
0	0	3	5	8	NM_139464.2	CG32306-RB, transcript variant B (CG32306), mRNA
0	0	3	5	8	NM_206242.1	CG32306-RD, transcript variant D (CG32306), mRNA
0	0	3	5	3	NM_168949.1	CG6395-RB, transcript variant B (Csp), mRNA
0	0	3	5	3	NM_079489.2	CG6395-RA, transcript variant A (Csp), mRNA
0	0	3	5	3	NM_168950.2	CG6395-RC, transcript variant C (Csp), mRNA
0	0	3	2	6	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	2	5	10	NM_168571.2	CG32133-RA (CG32133), mRNA
0	0	2	3	4	NM_142606.2	CG10889-RA (CG10889), mRNA
0	0	2	2	3	NM_079845.2	CG7951-RA (sima), mRNA
0	0	1	5	6	NM_140735.1	CG6311-RB (CG6311), mRNA
0	0	1	3	6	NM_170278.1	CG31082-RA (CG31082), mRNA
0	0	1	3	2	NM_142589.1	CG4468-RA (CG4468), mRNA
0	0	1	2	5	NM_137395.1	CG10936-RA, transcript variant A (CG10936), mRNA
0	0	1	2	5	NM_166242.1	CG10936-RB, transcript variant B (CG10936), mRNA
0	0	1	2	3	NM_168626.1	CG7439-RC, transcript variant C (AGO2), mRNA
0	0	1	2	3	NM_140518.1	CG7439-RB, transcript variant B (AGO2), mRNA
0	0	1	2	2	NM_141687.1	CG12946-RA, transcript variant A (CG12946), mRNA
0	0	1	2	2	NM_001043234.1	CG12946-RB, transcript variant B (CG12946), mRNA
0	0	0	7	9	NM_136816.1	CG13214-RA, transcript variant A (CG13214), mRNA
0	0	0	6	10	NM_140112.1	CG14165-RA (CG14165), mRNA
0	0	0	6	9	NM_135156.2	CG13991-RA (CG13991), mRNA
0	0	0	6	3	NM_142713.2	CG15503-RA (CheB93a), mRNA
0	0	0	5	11	NM_206395.2	CG6292-RA, transcript variant A (CycT), mRNA
0	0	0	5	11	NM_079403.3	CG6292-RB, transcript variant B (CycT), mRNA
0	0	0	5	8	NM_137759.3	CG30263-RA (CG30263), mRNA
0	0	0	5	6	NM_166173.2	CG6262-RC, transcript variant C (CG6262), mRNA
0	0	0	5	6	NM_137279.3	CG6262-RA, transcript variant A (CG6262), mRNA
0	0	0	5	6	NM_166172.3	CG6262-RB, transcript variant B (CG6262), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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