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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4651R-2 |
| Symbol |
RpL13 |
Full Name |
Ribosomal protein L13 |
| CG No |
CG4651 |
Old CG No |
CG4651 |
| Synonyms |
Dmbbc1, Rpl13, bbc1, unnamed, rpL13, anon-EST:fe1D1, CG4651, Bbc1, RpL13 |
| Accession No (Link to NCBI) |
NM_078806.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Schroeder AM, Allahyari M, Vogler G, Missinato MA, Nielsen T, Yu MS, Theis JL, Larsen LA, Goyal P, Rosenfeld JA, Nelson TJ, Olson TM, Colas AR, Grossfeld P, Bodmer R.<br> Model system identification of novel congenital heart disease gene candidates: focus on RPL13.<br> Hum Mol Genet (2019) 28(23) 3954-3969
[
PubMed ID = 31625562
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATTCCGAATCAGCACTACCAC |
| Primer Seq. 3' |
aatctagaggtaccTTCTCATCCTTGGTCACCTCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATTCCGAATC AGCACTACCA CAAGTGGTGG CAGCGGCATG TGAAAGACCT GGTTCAACCA
0061 GCCGGCCCGC AANGTCCGCA GGCATGCGAA CCGCGTCAAG AAGGCTAAGG CCGTCTTCCC
0121 CCGCCCAAGC CAGCGGTGCC CTGCGCCCTG TGGTTCGCTG CCCCACCATC CGCTACCACA
0181 CCAAGCTGCG TGCCGGCCGT GGTTTCACCC TGGAGGAGCT GAAGGGTGCC GGCATTGGCG
0241 CCAACTTCGC CAAGACCATC GGCATTGCCG TCGACAGGAG GCGCAAGAAC AAATCCCTGG
0301 AGTCCCGCCA GCGTAACATC CAGCGCCTCA AGGAGTACCG CAGCAAGTTG ATCCTGTTCC
0361 CCATCAACGA GAAGAAGATC CGCGCCGGCG AGTCCTCTCT GGAGGAGTGC AAGCTGGCTA
0421 CCCAGCTTAA GGGACCCGTC CTGCCCATCA AAAATGAGCA GCCCGCCGTG GTCGAGTTCC
0481 GTGAGGTGAC CAAGGATGAG AA |
| in silico PCR Fragment |
0001 ATTCCGAATC AGCACTACCA CAAGTGGTGG CAGCGGCATG TG-AAGACCT GGTTCAACCA
0061 GCCGGCCCGC AAGGTCCGCA GGCATGCGAA CCGCGTCAAG AAGGCTAAGG CCGTCTTCCC
0121 CCGCCC-AGC CAGCGGTGCC CTGCGCCCTG TGGTTCGCTG CCCCACCATC CGCTACCACA
0181 CCAAGCTGCG TGCCGGCCGT GGTTTCACCC TGGAGGAGCT GAAGGGTGCC GGCATTGGCG
0241 CCAACTTCGC CAAGACCATC GGCATTGCCG TCGACAGGAG GCGCAAGAAC AAATCCCTGG
0301 AGTCCCGCCA GCGTAACATC CAGCGCCTCA AGGAGTACCG CAGCAAGTTG ATCCTGTTCC
0361 CCATCAACGA GAAGAAGATC CGCGCCGGCG AGTCCTCTCT GGAGGAGTGC AAGCTGGCTA
0421 CCCAGCTTAA GGGACCCGTC CTGCCCATCA AAAATGAGCA GCCCGCCGTG GTCGAGTTCC
0481 GTGAGGTGAC CAAGGATGAG AA |
| Assemble Data |
<PRE>
4651R-2.IR_full 1 ATTCCGAATCAGCACTACCACAAGTGGTGGCAGCGGCATGTG<span class="snp"><tt>A</tt></span>AAGACCTGGTTCAACCA 60
||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||
4651R-2.in silico 1 ATTCCGAATCAGCACTACCACAAGTGGTGGCAGCGGCATGTG<span class="snp"><tt>-</tt></span>AAGACCTGGTTCAACCA 60
4651R-2.IR_full 61 GCCGGCCCGCAA<span class="snp"><tt>N</tt></span>GTCCGCAGGCATGCGAACCGCGTCAAGAAGGCTAAGGCCGTCTTCCC 120
||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
4651R-2.in silico 61 GCCGGCCCGCAA<span class="snp"><tt>G</tt></span>GTCCGCAGGCATGCGAACCGCGTCAAGAAGGCTAAGGCCGTCTTCCC 120
4651R-2.IR_full 121 CCGCCC<span class="snp"><tt>A</tt></span>AGCCAGCGGTGCCCTGCGCCCTGTGGTTCGCTGCCCCACCATCCGCTACCACA 180
||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
4651R-2.in silico 121 CCGCCC<span class="snp"><tt>-</tt></span>AGCCAGCGGTGCCCTGCGCCCTGTGGTTCGCTGCCCCACCATCCGCTACCACA 180
4651R-2.IR_full 181 CCAAGCTGCGTGCCGGCCGTGGTTTCACCCTGGAGGAGCTGAAGGGTGCCGGCATTGGCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4651R-2.in silico 181 CCAAGCTGCGTGCCGGCCGTGGTTTCACCCTGGAGGAGCTGAAGGGTGCCGGCATTGGCG 240
4651R-2.IR_full 241 CCAACTTCGCCAAGACCATCGGCATTGCCGTCGACAGGAGGCGCAAGAACAAATCCCTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4651R-2.in silico 241 CCAACTTCGCCAAGACCATCGGCATTGCCGTCGACAGGAGGCGCAAGAACAAATCCCTGG 300
4651R-2.IR_full 301 AGTCCCGCCAGCGTAACATCCAGCGCCTCAAGGAGTACCGCAGCAAGTTGATCCTGTTCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4651R-2.in silico 301 AGTCCCGCCAGCGTAACATCCAGCGCCTCAAGGAGTACCGCAGCAAGTTGATCCTGTTCC 360
4651R-2.IR_full 361 CCATCAACGAGAAGAAGATCCGCGCCGGCGAGTCCTCTCTGGAGGAGTGCAAGCTGGCTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4651R-2.in silico 361 CCATCAACGAGAAGAAGATCCGCGCCGGCGAGTCCTCTCTGGAGGAGTGCAAGCTGGCTA 420
4651R-2.IR_full 421 CCCAGCTTAAGGGACCCGTCCTGCCCATCAAAAATGAGCAGCCCGCCGTGGTCGAGTTCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4651R-2.in silico 421 CCCAGCTTAAGGGACCCGTCCTGCCCATCAAAAATGAGCAGCCCGCCGTGGTCGAGTTCC 480
4651R-2.IR_full 481 GTGAGGTGACCAAGGATGAGAA 502
||||||||||||||||||||||
4651R-2.in silico 481 GTGAGGTGACCAAGGATGAGAA 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
3 |
NM_078806.2 |
CG4651-RA, transcript variant A (RpL13), mRNA |
| 100 |
482 |
0 |
0 |
3 |
NM_001038802.1 |
CG4651-RB, transcript variant B (RpL13), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_166274.1 |
CG5729-RA, transcript variant A (Dgp-1), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_137458.2 |
CG5729-RB, transcript variant B (Dgp-1), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_169495.2 |
CG7771-RB, transcript variant B (sim), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_079616.2 |
CG7771-RA, transcript variant A (sim), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_137443.2 |
CG10912-RA (CG10912), mRNA |
| 0 |
0 |
2 |
8 |
17 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_144045.1 |
CG13551-RA, transcript variant A (CG13551), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_166597.1 |
CG13551-RB, transcript variant B (CG13551), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_166598.1 |
CG13551-RC, transcript variant C (CG13551), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_206492.1 |
CG33332-RA (CG33332), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_132645.2 |
CG15745-RA, transcript variant A (CG15745), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167357.1 |
CG15745-RB, transcript variant B (CG15745), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_132636.2 |
CG12723-RA (CG12723), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140043.3 |
CG4347-RA, transcript variant A (UGP), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168325.2 |
CG4347-RC, transcript variant C (UGP), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_140159.1 |
CG7949-RA (CG7949), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_079572.2 |
CG9484-RA (hyd), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_206154.1 |
CG33130-RB, transcript variant B (l(2)k07433), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_176218.1 |
CG33130-RA, transcript variant A (l(2)k07433), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_206153.1 |
CG33130-RC, transcript variant C (l(2)k07433), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_140461.2 |
CG13471-RA (CG13471), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_206006.2 |
CG10604-RB, transcript variant B (bsh), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_058002.2 |
CG10604-RA, transcript variant A (bsh), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_141207.3 |
CG9853-RA (CG9853), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132423.1 |
CG11556-RA (Rph), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_139922.2 |
CG13676-RA (CG13676), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136692.1 |
CG12140-RA (CG12140), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_079903.2 |
CG15319-RB (nej), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme03010
:
Ribosome
|
|
|
