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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4636R-1 |
| Symbol |
SCAR |
Full Name |
SCAR |
| CG No |
CG4636 |
Old CG No |
CG4636 |
| Synonyms |
Wave, Scar, scar, wave, l(2)k03107, WAVE, CG4636, unnamed, D-SCAR, l(2)k13811, BEST:SD02991, SCAR |
| Accession No (Link to NCBI) |
NM_135633.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Rüder M, Nagel BM, Bogdan S.
Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo.
Methods Mol. Biol. (2018) 1749 227-238
[
PubMed ID = 29526001
]
[
RRC reference
]
Stephan R, Gohl C, Fleige A, Klämbt C, Bogdan S.
Membrane-targeted WAVE mediates photoreceptor axon targeting in the absence of the WAVE complex in Drosophila.
Mol. Biol. Cell (2011) 22(21) 4079-92
[
PubMed ID = 21900504
]
[
RRC reference
]
Chen XJ, Squarr AJ, Stephan R, Chen B, Higgins TE, Barry DJ, Martin MC, Rosen MK, Bogdan S, Way M.
Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton.
Dev. Cell (2014) 30(5) 569-84
[
PubMed ID = 25203209
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev. Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCGCTGCCCAAACGATCCATA |
| Primer Seq. 3' |
aatctagaggtaccAGCATCTCCTGTCGCCACAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCGCTGCCCA AACGATCCAT AGAACCCGTG CATGTGGCCC GCTCCGTGTA TCAGCAGGAT
0061 GAGCTGCAAT CCGTGGAGCT GGAGACGGTC ACCAACACCA CGCTGACGAA CATCATTCGC
0121 CAGCTGTCCT CGCTGTCCAA GCACGCAGAG GATGTGTTCG GTGAACTGGC CCGCGACGTG
0181 GGCAACATCG GGGATCGAGC TAACTCCCTG CAGGCGCGCA TCGATCGCCT AGCTATCAAA
0241 GTTACCCAGC TGGACAGCAC AGTTGAGGAG GTGCCCTTGA CGGACATTAC CCGAAAGAAG
0301 GCCTTCAAGT CGGCCAAGGT TTTTGATCAA CAGATCTTCT CGCGCGCCAC CATGCCGGCA
0361 CCAATGATGG ACACATATGC CCAGTGCGAC AAACCGCCGC CCCTCGACAA GCTTAATGTC
0421 TATCGAGACG ATGGCAAGGA TGGTCTTAAG TTCTACACGG ATCCGAACTA CTTCTTTGAG
0481 TTGTGGCGAC AGGAGATGCT |
| in silico PCR Fragment |
0001 CCGCTGCCCA AACGATCCAT AGAACCCGTG CATGTGGCCC GCTCCGTGTA TCAGCAGGAT
0061 GAGCTGCAAT CCGTGGAGCT GGAGACGGTC ACCAACACCA CGCTGACGAA CATCATTCGC
0121 CAGCTGTCCT CGCTGTCCAA GCACGCAGAG GATGTGTTCG GTGAACTGGC CCGCGACGTG
0181 GGCAACATCG GGGATCGAGC TAACTCCCTG CAGGCGCGCA TCGATCGCCT AGCTATCAAA
0241 GTTACCCAGC TGGACAGCAC AGTTGAGGAG GTGCCCTTGA CGGACATTAC CCGAAAGAAG
0301 GCCTTCAAGT CGGCCAAGGT TTTTGATCAA CAGATCTTCT CGCGCGCCAC CATGCCGGCA
0361 CCAATGATGG ACACATATGC CCAGTGCGAC AAACCGCCGC CCCTCGACAA GCTTAATGTC
0421 TATCGAGACG ATGGCAAGGA TGGTCTTAAG TTCTACACGG ATCCGAACTA CTTCTTTGAG
0481 TTGTGGCGAC AGGAGATGCT |
| Assemble Data |
4636R-1.IR_full 1 CCGCTGCCCAAACGATCCATAGAACCCGTGCATGTGGCCCGCTCCGTGTATCAGCAGGAT 60
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4636R-1.in silico 1 CCGCTGCCCAAACGATCCATAGAACCCGTGCATGTGGCCCGCTCCGTGTATCAGCAGGAT 60
4636R-1.IR_full 61 GAGCTGCAATCCGTGGAGCTGGAGACGGTCACCAACACCACGCTGACGAACATCATTCGC 120
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4636R-1.in silico 61 GAGCTGCAATCCGTGGAGCTGGAGACGGTCACCAACACCACGCTGACGAACATCATTCGC 120
4636R-1.IR_full 121 CAGCTGTCCTCGCTGTCCAAGCACGCAGAGGATGTGTTCGGTGAACTGGCCCGCGACGTG 180
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4636R-1.in silico 121 CAGCTGTCCTCGCTGTCCAAGCACGCAGAGGATGTGTTCGGTGAACTGGCCCGCGACGTG 180
4636R-1.IR_full 181 GGCAACATCGGGGATCGAGCTAACTCCCTGCAGGCGCGCATCGATCGCCTAGCTATCAAA 240
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4636R-1.in silico 181 GGCAACATCGGGGATCGAGCTAACTCCCTGCAGGCGCGCATCGATCGCCTAGCTATCAAA 240
4636R-1.IR_full 241 GTTACCCAGCTGGACAGCACAGTTGAGGAGGTGCCCTTGACGGACATTACCCGAAAGAAG 300
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4636R-1.in silico 241 GTTACCCAGCTGGACAGCACAGTTGAGGAGGTGCCCTTGACGGACATTACCCGAAAGAAG 300
4636R-1.IR_full 301 GCCTTCAAGTCGGCCAAGGTTTTTGATCAACAGATCTTCTCGCGCGCCACCATGCCGGCA 360
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4636R-1.in silico 301 GCCTTCAAGTCGGCCAAGGTTTTTGATCAACAGATCTTCTCGCGCGCCACCATGCCGGCA 360
4636R-1.IR_full 361 CCAATGATGGACACATATGCCCAGTGCGACAAACCGCCGCCCCTCGACAAGCTTAATGTC 420
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4636R-1.in silico 361 CCAATGATGGACACATATGCCCAGTGCGACAAACCGCCGCCCCTCGACAAGCTTAATGTC 420
4636R-1.IR_full 421 TATCGAGACGATGGCAAGGATGGTCTTAAGTTCTACACGGATCCGAACTACTTCTTTGAG 480
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4636R-1.in silico 421 TATCGAGACGATGGCAAGGATGGTCTTAAGTTCTACACGGATCCGAACTACTTCTTTGAG 480
4636R-1.IR_full 481 TTGTGGCGACAGGAGATGCT 500
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4636R-1.in silico 481 TTGTGGCGACAGGAGATGCT 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_135633.2 |
CG4636-RA (SCAR), mRNA |
| 0 |
0 |
5 |
2 |
2 |
NM_165848.1 |
CG12444-RA, transcript variant A (Tango3), mRNA |
| 0 |
0 |
5 |
2 |
2 |
NM_136856.1 |
CG12444-RB, transcript variant B (Tango3), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_139579.2 |
CG10849-RA (Sc2), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_169463.1 |
CG31218-RA (CG31218), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166560.1 |
CG30271-RC (CG30271), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_169573.1 |
CG12207-RA, transcript variant A (CG12207), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_169572.1 |
CG12207-RC, transcript variant C (CG12207), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_142095.2 |
CG12207-RB, transcript variant B (CG12207), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_142869.3 |
CG4656-RA (CG4656), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_001015398.1 |
CG41125-PA (CG41125), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_132092.2 |
CG3869-RA, transcript variant A (Marf), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_206634.1 |
CG3869-RC, transcript variant C (Marf), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_206635.2 |
CG3869-RB, transcript variant B (Marf), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_205889.1 |
CG31665-RB, transcript variant B (CG31665), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_164443.1 |
CG31665-RA, transcript variant A (CG31665), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001038844.1 |
CG31693-RB, transcript variant B (CG31693), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165402.2 |
CG31693-RA, transcript variant A (CG31693), mRNA |
| 0 |
0 |
0 |
8 |
12 |
NM_140271.1 |
CG17826-RA (CG17826), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_132068.1 |
CG15899-RB (Ca-alpha1T), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_139965.2 |
CG7127-RA (exo70), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_133034.3 |
CG6867-RA (CG6867), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_166973.1 |
CG12206-RB, transcript variant B (CG12206), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_130704.1 |
CG12206-RA, transcript variant A (CG12206), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_001043070.1 |
CG34146-RA (brp), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_166974.1 |
CG12206-RC, transcript variant C (CG12206), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135846.2 |
CG16884-RA (CG16884), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_169170.1 |
CG1070-RC, transcript variant C (Alh), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079526.2 |
CG1070-RA, transcript variant A (Alh), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_169172.1 |
CG1070-RB, transcript variant B (Alh), mRNA |
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