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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4427R-2

Symbol cbt Full Name cabut

CG No CG4427 Old CG No CG4427

Synonyms CG4427, EP2237, EP(2)2237, dTIEG, cbt

Accession No (Link to NCBI) NM_164384.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Belacortu Y, Weiss R, Kadener S, Paricio N.
Expression of Drosophila Cabut during early embryogenesis, dorsal closure and nervous system development.
Gene Expr. Patterns (2011) 11(3-4) 190-201 [ PubMed ID = 21109026 ] [ RRC reference ]

Fairchild MJ, Islam F, Tanentzapf G.
Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
PLoS Genet. (2017) 13(9) e1007026 [ PubMed ID = 28957323 ] [ RRC reference ]

Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev. Biol. (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATACCTTGCTACCTTCACCAC

Primer Seq. 3'

aatctagaggtaccTTCTGCAGCACGCTTTCTGTT

Predicted Fragment Size

IR fragment full Seq

0001 ATACCTTGCT ACCTTCACCA CCCGCAACgN CCCCACTAAG GGAAAACAAG TTGGAAATCG 
0061 TCGCCAAGGA CGAACAACAG GTGAACGAGA ATCTGCTAAA GGCAAAGCTC AANGCTGGTG 
0121 GCCCAAAAGA GTCAGAAAAA ATGGAGGGAT TATTACACCA AATCCATCGG ACACGGGAAG 
0181 ACGAGGCTCC CCGAAATNAG CGGTTCCCAA CAAGAAACCC CGTTTGGAAC AACTTGCTAT 
0241 GAGCATGACA CCGCCGCCCG GATCAAAAGC TGGACGATGA TCAGAAAGCG GAAAGGGTTA 
0301 GCGTCATCAT GCGGGTCAAC AGTTCTGGCG CTGTCTCTTC TAGTAGCCAA GACGAGAACT 
0361 CATCTAGTTC CACCTCCTGC TGTAGTTCCT CTTCCAACAC AAACACAAGT ACAAGTTCAG 
0421 TACCACCCAC TGTGGAGGAC GACTATCCAG AGGCCAATGT GTGGCGCAAT CTCAAGTTCA 
0481 AAATGAACAG AAAGCGTGCT GCAGAA

in silico PCR Fragment

0001 ATACCTTGCT ACCTTCACCA CCCGCAACGC CCCCACTAAG GGAAAACAAG TTGGAAATCG 
0061 TCGCCAAGGA CGAACAACAG GTGAACGAGA ATCTGCTAAA GGCAAAGCTC AA-GCTGGTG 
0121 GCCCAAAAGA GTCAG-AAAA ATGGAGGGAT TATTACACCA AATCCATCGG ACAC-GGAAG 
0181 ACGAGGCT-C CCGAAAT-AG CGGTTCCCAA CAAGAAACCC CGTTTGGAAC AACCTGCTAT 
0241 GAGCATGACA CCGCCG-CCG GATCAAAAGC TGGACGATGA TCAGAAAGCG GAAAGGGTTA 
0301 GCGTCATCAT GCGGGTCAAC AGTTCTGGCG CTGTCTCTTC TAGTAGCCAA GACGAGAACT 
0361 CATCTAGTTC CACCTCCTGC TGTAGTTCCT CTTCCAACAC AAACACAAGT ACAAGTTCAG 
0421 TACCACCCAC TGTGGAGGAC GACTATCCAG AGGCCAATGT GTGGCGCAAT CTCAAGTTCA 
0481 AAATGAACAG AAAGCGTGCT GCAGAA

Assemble Data


4427R-2.IR_full       1   ATACCTTGCTACCTTCACCACCCGCAACGNCCCCACTAAGGGAAAACAAGTTGGAAATCG 60
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
4427R-2.in silico     1   ATACCTTGCTACCTTCACCACCCGCAACGCCCCCACTAAGGGAAAACAAGTTGGAAATCG 60


4427R-2.IR_full       61  TCGCCAAGGACGAACAACAGGTGAACGAGAATCTGCTAAAGGCAAAGCTCAANGCTGGTG 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
4427R-2.in silico     61  TCGCCAAGGACGAACAACAGGTGAACGAGAATCTGCTAAAGGCAAAGCTCAA-GCTGGTG 120


4427R-2.IR_full       121 GCCCAAAAGAGTCAGAAAAAATGGAGGGATTATTACACCAAATCCATCGGACACGGGAAG 180
                          ||||||||||||||| |||||||||||||||||||||||||||||||||||||| |||||
4427R-2.in silico     121 GCCCAAAAGAGTCAG-AAAAATGGAGGGATTATTACACCAAATCCATCGGACAC-GGAAG 180


4427R-2.IR_full       181 ACGAGGCTCCCCGAAATNAGCGGTTCCCAACAAGAAACCCCGTTTGGAACAACTTGCTAT 240
                          |||||||| |||||||| ||||||||||||||||||||||||||||||||||| ||||||
4427R-2.in silico     181 ACGAGGCT-CCCGAAAT-AGCGGTTCCCAACAAGAAACCCCGTTTGGAACAACCTGCTAT 240


4427R-2.IR_full       241 GAGCATGACACCGCCGCCCGGATCAAAAGCTGGACGATGATCAGAAAGCGGAAAGGGTTA 300
                          |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
4427R-2.in silico     241 GAGCATGACACCGCCG-CCGGATCAAAAGCTGGACGATGATCAGAAAGCGGAAAGGGTTA 300


4427R-2.IR_full       301 GCGTCATCATGCGGGTCAACAGTTCTGGCGCTGTCTCTTCTAGTAGCCAAGACGAGAACT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4427R-2.in silico     301 GCGTCATCATGCGGGTCAACAGTTCTGGCGCTGTCTCTTCTAGTAGCCAAGACGAGAACT 360


4427R-2.IR_full       361 CATCTAGTTCCACCTCCTGCTGTAGTTCCTCTTCCAACACAAACACAAGTACAAGTTCAG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4427R-2.in silico     361 CATCTAGTTCCACCTCCTGCTGTAGTTCCTCTTCCAACACAAACACAAGTACAAGTTCAG 420


4427R-2.IR_full       421 TACCACCCACTGTGGAGGACGACTATCCAGAGGCCAATGTGTGGCGCAATCTCAAGTTCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4427R-2.in silico     421 TACCACCCACTGTGGAGGACGACTATCCAGAGGCCAATGTGTGGCGCAATCTCAAGTTCA 480


4427R-2.IR_full       481 AAATGAACAGAAAGCGTGCTGCAGAA 506
                          ||||||||||||||||||||||||||
4427R-2.in silico     481 AAATGAACAGAAAGCGTGCTGCAGAA 506

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_164384.1	CG4427-RA, transcript variant A (cbt), mRNA
87.75	423	1	1	1	NM_134685.2	CG4427-RB, transcript variant B (cbt), mRNA
0	0	2	4	8	NM_141003.2	CG3634-RA (CG3634), mRNA
0	0	2	4	3	NM_140561.3	CG5931-RA (CG5931), mRNA
0	0	2	3	2	NM_136598.2	CG8196-RA (Ance-4), mRNA
0	0	2	2	4	NM_138158.2	CG1212-RA, transcript variant A (p130CAS), mRNA
0	0	2	2	3	NM_136021.2	CG15152-RA (CG15152), mRNA
0	0	1	5	2	NM_139461.1	CG15877-RA (CG15877), mRNA
0	0	1	4	4	NM_134479.1	CG14205-RA (CG14205), mRNA
0	0	1	4	4	NM_144347.2	CG12149-RA (c12.2), mRNA
0	0	1	4	3	NM_166612.2	CG30182-RA (CG30182), mRNA
0	0	1	3	6	NM_165934.1	CG8776-RA, transcript variant A (CG8776), mRNA
0	0	1	3	3	NM_136702.2	CG18445-RA (CG18445), mRNA
0	0	1	3	3	NM_080214.2	CG10913-RA (Spn6), mRNA
0	0	1	3	2	NM_134494.2	CG12235-RA (Arp11), mRNA
0	0	1	2	3	NM_167380.1	CG32628-RA (CG32628), mRNA
0	0	1	2	3	NM_001042932.1	CG17494-RB, transcript variant B (CG17494), mRNA
0	0	1	2	3	NM_001042933.1	CG17494-RC, transcript variant C (CG17494), mRNA
0	0	1	2	3	NM_001042934.1	CG17494-RA, transcript variant A (CG17494), mRNA
0	0	1	2	2	NM_139856.2	CG8580-RA, transcript variant A (bhr), mRNA
0	0	1	2	2	NM_168211.1	CG8580-RB, transcript variant B (bhr), mRNA
0	0	1	2	2	NM_079413.2	CG4345-RA (grim), mRNA
0	0	0	9	12	NM_079067.2	CG11949-RA, transcript variant A (cora), mRNA
0	0	0	8	4	NM_169578.1	CG3307-RC, transcript variant C (pr-set7), mRNA
0	0	0	8	4	NM_169577.1	CG3307-RB, transcript variant B (pr-set7), mRNA
0	0	0	8	4	NM_142097.2	CG3307-RA, transcript variant A (pr-set7), mRNA
0	0	0	5	6	NM_169845.1	CG31043-RA, transcript variant A (gukh), mRNA
0	0	0	4	10	NM_057511.3	CG3936-RA (N), mRNA
0	0	0	4	10	NM_132529.2	CG2025-RA (CG2025), mRNA
0	0	0	4	9	NM_166337.1	CG11949-RB, transcript variant B (cora), mRNA

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