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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4376R-1 |
| Symbol |
Actn |
Full Name |
alpha actinin |
| CG No |
CG4376 |
Old CG No |
CG4376 |
| Synonyms |
l(1)2Cb, fliA, actn, EG:133E12.1, CG4376, CT14232, CT14163, HM-29, GA17, l(1)EA82, alpha-actinin, y, Actn |
| Accession No (Link to NCBI) |
NM_058136.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTACATGGAGCAGGAGGAGGAG |
| Primer Seq. 3' |
aatctagaggtaccGCCAGACCATCCTTGAACGAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TACATGGAGC AGGAGGAGGA GTGGGAGCGC GAGGGCCTGC TCGATCCTGC GTGGGAGAAG
0061 CAACAGAAGA AGACATTTAC CGCCTGGTGT AACAGCCATT TGCGCAAGGC CGGTACAGCC
0121 ATCGACAACA TCGAGGAGGA CTTCCGCAAC GGCCTCAAGC TGATGCTGCT GCTGGAGGTG
0181 ATCTCCGGCG AGACGCTCCC CAAGCCCGAT CGCGGCAAGA TGCGCTTCCA CAAGATCGCC
0241 AACGTGAACA AGGCTCTCGA CTTCATCGCC TCCAAGGGCG TCCACCTGGT CTCTATCGGC
0301 GCCGAGGAGA TCGTCGATGG AAACTTGAAG ATGACTCTGG GTATGATCTG GACGATTATC
0361 CTGCGCTTCG CCATTCAGGA CATCTCCGTG GAGGAGATGA CCGCCAAGGA GGGTCTGCTG
0421 CTGTGGTGCC AGCGCAAGAC GGCTCCCTAC AAGAACGTCA ACGTACAGAA CTTCCATCTG
0481 TCGTTCAAGG ATGGTCTGGC |
| in silico PCR Fragment |
0001 TACATGGAGC AGGAGGAGGA GTGGGAGCGC GAGGGCCTGC TCGATCCTGC GTGGGAGAAG
0061 CAACAGAAGA AGACATTTAC CGCCTGGTGT AACAGCCATT TGCGCAAGGC CGGTACAGCC
0121 ATCGACAACA TCGAGGAGGA CTTCCGCAAC GGCCTCAAGC TGATGCTGCT GCTGGAGGTG
0181 ATCTCCGGCG AGACGCTCCC CAAGCCCGAT CGCGGCAAGA TGCGCTTCCA CAAGATCGCC
0241 AACGTGAACA AGGCTCTCGA CTTCATCGCC TCCAAGGGCG TCCACCTGGT CTCTATCGGC
0301 GCCGAGGAGA TCGTCGATGG AAACTTGAAG ATGACTCTGG GTATGATCTG GACGATTATC
0361 CTGCGCTTCG CCATTCAGGA CATCTCCGTG GAGGAGATGA CCGCCAAGGA GGGTCTGCTG
0421 CTGTGGTGCC AGCGCAAGAC GGCTCCCTAC AAGAACGTCA ACGTACAGAA CTTCCATCTG
0481 TCGTTCAAGG ATGGTCTGGC |
| Assemble Data |
<PRE>
4376R-1.IR_full 1 TACATGGAGCAGGAGGAGGAGTGGGAGCGCGAGGGCCTGCTCGATCCTGCGTGGGAGAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4376R-1.in silico 1 TACATGGAGCAGGAGGAGGAGTGGGAGCGCGAGGGCCTGCTCGATCCTGCGTGGGAGAAG 60
4376R-1.IR_full 61 CAACAGAAGAAGACATTTACCGCCTGGTGTAACAGCCATTTGCGCAAGGCCGGTACAGCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4376R-1.in silico 61 CAACAGAAGAAGACATTTACCGCCTGGTGTAACAGCCATTTGCGCAAGGCCGGTACAGCC 120
4376R-1.IR_full 121 ATCGACAACATCGAGGAGGACTTCCGCAACGGCCTCAAGCTGATGCTGCTGCTGGAGGTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4376R-1.in silico 121 ATCGACAACATCGAGGAGGACTTCCGCAACGGCCTCAAGCTGATGCTGCTGCTGGAGGTG 180
4376R-1.IR_full 181 ATCTCCGGCGAGACGCTCCCCAAGCCCGATCGCGGCAAGATGCGCTTCCACAAGATCGCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4376R-1.in silico 181 ATCTCCGGCGAGACGCTCCCCAAGCCCGATCGCGGCAAGATGCGCTTCCACAAGATCGCC 240
4376R-1.IR_full 241 AACGTGAACAAGGCTCTCGACTTCATCGCCTCCAAGGGCGTCCACCTGGTCTCTATCGGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4376R-1.in silico 241 AACGTGAACAAGGCTCTCGACTTCATCGCCTCCAAGGGCGTCCACCTGGTCTCTATCGGC 300
4376R-1.IR_full 301 GCCGAGGAGATCGTCGATGGAAACTTGAAGATGACTCTGGGTATGATCTGGACGATTATC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4376R-1.in silico 301 GCCGAGGAGATCGTCGATGGAAACTTGAAGATGACTCTGGGTATGATCTGGACGATTATC 360
4376R-1.IR_full 361 CTGCGCTTCGCCATTCAGGACATCTCCGTGGAGGAGATGACCGCCAAGGAGGGTCTGCTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4376R-1.in silico 361 CTGCGCTTCGCCATTCAGGACATCTCCGTGGAGGAGATGACCGCCAAGGAGGGTCTGCTG 420
4376R-1.IR_full 421 CTGTGGTGCCAGCGCAAGACGGCTCCCTACAAGAACGTCAACGTACAGAACTTCCATCTG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4376R-1.in silico 421 CTGTGGTGCCAGCGCAAGACGGCTCCCTACAAGAACGTCAACGTACAGAACTTCCATCTG 480
4376R-1.IR_full 481 TCGTTCAAGGATGGTCTGGC 500
||||||||||||||||||||
4376R-1.in silico 481 TCGTTCAAGGATGGTCTGGC 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_058136.3 |
CG4376-RA, transcript variant A (Actn), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_058137.3 |
CG4376-RC, transcript variant C (Actn), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_166920.1 |
CG4376-RB, transcript variant B (Actn), mRNA |
| 0.62 |
3 |
7 |
9 |
27 |
NM_079176.1 |
CG12008-RA, transcript variant A (kst), mRNA |
| 0.62 |
3 |
7 |
9 |
27 |
NM_206266.1 |
CG12008-RB, transcript variant B (kst), mRNA |
| 0.62 |
3 |
7 |
9 |
27 |
NM_206265.1 |
CG12008-RC, transcript variant C (kst), mRNA |
| 0.41 |
2 |
3 |
3 |
4 |
NM_142122.2 |
CG7832-RA (CG7832), mRNA |
| 0 |
0 |
5 |
8 |
20 |
NM_140402.2 |
CG17689-RA, transcript variant A (CG17689), mRNA |
| 0 |
0 |
5 |
4 |
11 |
NM_142889.1 |
CG4374-RA (CG4374), mRNA |
| 0 |
0 |
5 |
2 |
12 |
NM_078827.1 |
CG4977-RA (kek2), mRNA |
| 0 |
0 |
4 |
8 |
41 |
NM_001014500.1 |
CG33558-RA (CG33558), mRNA |
| 0 |
0 |
4 |
5 |
14 |
NM_132606.1 |
CG3754-RA (CG3754), mRNA |
| 0 |
0 |
4 |
3 |
14 |
NM_001038916.1 |
CG17689-RB, transcript variant B (CG17689), mRNA |
| 0 |
0 |
4 |
3 |
8 |
NM_143352.1 |
CG12870-RA (CG12870), mRNA |
| 0 |
0 |
3 |
11 |
36 |
NM_170258.2 |
CG32474-RA (dys), mRNA |
| 0 |
0 |
3 |
7 |
23 |
NM_057891.2 |
CG3365-RB, transcript variant B (drongo), mRNA |
| 0 |
0 |
3 |
7 |
23 |
NM_164392.1 |
CG3365-RC, transcript variant C (drongo), mRNA |
| 0 |
0 |
3 |
7 |
23 |
NM_164391.1 |
CG3365-RA, transcript variant A (drongo), mRNA |
| 0 |
0 |
3 |
7 |
13 |
NM_167844.1 |
CG32479-RA (CG32479), mRNA |
| 0 |
0 |
3 |
5 |
13 |
NM_170659.1 |
CG7835-RA (CG7835), mRNA |
| 0 |
0 |
3 |
5 |
7 |
NM_132272.1 |
CG11294-RA (CG11294), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_135110.1 |
CG11147-RA, transcript variant A (CG11147), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_164658.1 |
CG11147-RB, transcript variant B (CG11147), mRNA |
| 0 |
0 |
3 |
3 |
6 |
NM_168881.1 |
CG32434-RB, transcript variant B (siz), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_206407.1 |
CG32434-RA, transcript variant A (siz), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_001043153.1 |
CG32434-RC, transcript variant C (siz), mRNA |
| 0 |
0 |
2 |
11 |
8 |
NM_165836.1 |
CG7776-RB, transcript variant B (E(Pc)), mRNA |
| 0 |
0 |
2 |
11 |
8 |
NM_078974.2 |
CG7776-RA, transcript variant A (E(Pc)), mRNA |
| 0 |
0 |
2 |
6 |
64 |
NM_001038734.1 |
CG16902-RC (Hr4), mRNA |
| 0 |
0 |
2 |
6 |
47 |
NM_166873.1 |
CG3638-RB, transcript variant B (CG3638), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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