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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4353R-2 |
| Symbol |
hep |
Full Name |
hemipterous |
| CG No |
CG4353 |
Old CG No |
CG4353 |
| Synonyms |
HEP/MKK7, DJNKK, HEP, JNKK, MKK7, DHEP/MKK7, CG2190, CG4353, DMKK7, hp, l(1)G0107, l(1)G0208, l(1)7P1, hem, hep, Hep |
| Accession No (Link to NCBI) |
NM_167346.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Chang YC, Tu H, Chen JY, Chang CC, Yang SY, Pi H.
Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
PLoS Genet (2019) 15(7) e1008062
[
PubMed ID = 31295251
]
[
RRC reference
]
Clavier A, Rincheval-Arnold A, Baillet A, Mignotte B, Guénal I.
Two different specific JNK activators are required to trigger apoptosis or compensatory proliferation in response to Rbf1 in Drosophila.
Cell Cycle (2016) 15(2) 283-94
[
PubMed ID = 26825229
]
[
RRC reference
]
Clavier A, Ruby V, Rincheval-Arnold A, Mignotte B, Guénal I.
The Drosophila retinoblastoma protein, Rbf1, induces a Debcl- and Drp1-dependent mitochondrial apoptosis.
J Cell Sci (2015) 128(17) 3239-49
[
PubMed ID = 26208635
]
[
RRC reference
]
Demay Y, Perochon J, Szuplewski S, Mignotte B, Gaumer S.
The PERK pathway independently triggers apoptosis and a Rac1/Slpr/JNK/Dilp8 signaling favoring tissue homeostasis in a chronic ER stress Drosophila model.
Cell Death Dis (2014) 5(10) e1452
[
PubMed ID = 25299777
]
[
RRC reference
]
Neisch AL, Speck O, Stronach B, Fehon RG.
Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
J Cell Biol (2010) 189(2) 311-23
[
PubMed ID = 20404112
]
[
RRC reference
]
Marchal C, Vinatier G, Sanial M, Plessis A, Pret AM, Limbourg-Bouchon B, Théodore L, Netter S.
The HIV-1 Vpu protein induces apoptosis in Drosophila via activation of JNK signaling.
PLoS One (2012) 7(3) e34310
[
PubMed ID = 22479597
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Ishimaru S, Ueda R, Hinohara Y, Ohtani M, Hanafusa H.
PVR plays a critical role via JNK activation in thorax closure during Drosophila metamorphosis.
EMBO J (2004) 23(20) 3984-94
[
PubMed ID = 15457211
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACCTTCCTTGGGCGCAGGTTC |
| Primer Seq. 3' |
aatctagaggtaccCAGTGCGTCGCATCTGCTTCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACCTTCCTTG GGCGCAGGTT CCGTTTCCGG ATCGGGTATC AGCATAGCCC AGCGTCCAGN
0061 ACACCTCCAG TTCCTCATTG CGACNCCCTT CGGCAGCGCC AGCGCCTCGT CATCATCCTC
0121 ATCCGCATCC GCATTTGCAT CCGCAGCACC GGCGACGNGC ACCTNTCGGT GGAACATACA
0181 CCCCGCCGAC AACTAGAGTG TCGCGAGCCA CGCCCACACT GCCCATGCTT TCTAGTGGTC
0241 CCGGTGGTGG ACTGAATCGT ACGCGGCCGG TGATACTTCC GCTGCCCACG CCGCCACATC
0301 CTCCAGTTTC GGAAACGGAC ATGAAGCTGA AGATCATCAT GGAGCAGACC GGCAAGCTGA
0361 ACATCAACGG GCGGCAGTAT CCGACGGACA TCAATGACCT CAAGCACCTG GGCGACCTGG
0421 GCAATGGGAC TAGCGGCAAT GTGGTGAAGA TGATGCACCT GTCCAGCAAC ACGATCATCG
0481 CCGTGAAGCA GATgNGACGC ACTG |
| in silico PCR Fragment |
0001 ACCTTCCTTG GGCGCAGGTT CCGTTTCCGG ATCGGGTATC AGCATAGCCC AGCGTCCAG-
0061 -CACCTCCAG TTCCTCA-TG CGACGCCCTT CGGCAGCGCC AGCGCCTCGT CATCATCCTC
0121 ATCCGCATCC GCATTTGCAT CCGCAGCACC GGCGACGGGC ACCT-TCGGT GGAACATACA
0181 CCCCGCCGAC AACTAGAGTG TCGCGAGCCA CGCCCACACT GCCCATGCTT TCTAGTGGTC
0241 CCGGTGGTGG ACTGAATCGT ACGCGGCCGG TGATACTTCC GCTGCCCACG CCGCCACATC
0301 CTCCAGTTTC GGAAACGGAC ATGAAGCTGA AGATCATCAT GGAGCAGACC GGCAAGCTGA
0361 ACATCAACGG GCGGCAGTAT CCGACGGACA TCAATGACCT CAAGCACCTG GGCGACCTGG
0421 GCAATGGGAC TAGCGGCAAT GTGGTGAAGA TGATGCACCT GTCCAGCAAC ACGATCATCG
0481 CCGTGAAGCA GATGCGACGC ACTG |
| Assemble Data |
4353R-2.IR_full 1 ACCTTCCTTGGGCGCAGGTTCCGTTTCCGGATCGGGTATCAGCATAGCCCAGCGTCCAGN 60
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4353R-2.in silico 1 ACCTTCCTTGGGCGCAGGTTCCGTTTCCGGATCGGGTATCAGCATAGCCCAGCGTCCAG- 60
4353R-2.IR_full 61 ACACCTCCAGTTCCTCATTGCGACNCCCTTCGGCAGCGCCAGCGCCTCGTCATCATCCTC 120
|||||||||||||||| |||||| |||||||||||||||||||||||||||||||||||
4353R-2.in silico 61 -CACCTCCAGTTCCTCA-TGCGACGCCCTTCGGCAGCGCCAGCGCCTCGTCATCATCCTC 120
4353R-2.IR_full 121 ATCCGCATCCGCATTTGCATCCGCAGCACCGGCGACGNGCACCTNTCGGTGGAACATACA 180
||||||||||||||||||||||||||||||||||||| |||||| |||||||||||||||
4353R-2.in silico 121 ATCCGCATCCGCATTTGCATCCGCAGCACCGGCGACGGGCACCT-TCGGTGGAACATACA 180
4353R-2.IR_full 181 CCCCGCCGACAACTAGAGTGTCGCGAGCCACGCCCACACTGCCCATGCTTTCTAGTGGTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4353R-2.in silico 181 CCCCGCCGACAACTAGAGTGTCGCGAGCCACGCCCACACTGCCCATGCTTTCTAGTGGTC 240
4353R-2.IR_full 241 CCGGTGGTGGACTGAATCGTACGCGGCCGGTGATACTTCCGCTGCCCACGCCGCCACATC 300
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4353R-2.in silico 241 CCGGTGGTGGACTGAATCGTACGCGGCCGGTGATACTTCCGCTGCCCACGCCGCCACATC 300
4353R-2.IR_full 301 CTCCAGTTTCGGAAACGGACATGAAGCTGAAGATCATCATGGAGCAGACCGGCAAGCTGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4353R-2.in silico 301 CTCCAGTTTCGGAAACGGACATGAAGCTGAAGATCATCATGGAGCAGACCGGCAAGCTGA 360
4353R-2.IR_full 361 ACATCAACGGGCGGCAGTATCCGACGGACATCAATGACCTCAAGCACCTGGGCGACCTGG 420
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4353R-2.in silico 361 ACATCAACGGGCGGCAGTATCCGACGGACATCAATGACCTCAAGCACCTGGGCGACCTGG 420
4353R-2.IR_full 421 GCAATGGGACTAGCGGCAATGTGGTGAAGATGATGCACCTGTCCAGCAACACGATCATCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4353R-2.in silico 421 GCAATGGGACTAGCGGCAATGTGGTGAAGATGATGCACCTGTCCAGCAACACGATCATCG 480
4353R-2.IR_full 481 CCGTGAAGCAGATGNGACGCACTG 504
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4353R-2.in silico 481 CCGTGAAGCAGATGCGACGCACTG 504
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
26 |
NM_167346.1 |
CG4353-RA, transcript variant A (hep), mRNA |
| 100 |
482 |
0 |
2 |
24 |
NM_078587.2 |
CG4353-RC, transcript variant C (hep), mRNA |
| 0.62 |
3 |
4 |
7 |
34 |
NM_001032002.1 |
CG33676-RA, transcript variant A (Skeletor), mRNA |
| 0.62 |
3 |
4 |
7 |
34 |
NM_001032000.1 |
CG14681-RB, transcript variant B (CG14681), mRNA |
| 0.2 |
1 |
5 |
4 |
2 |
NM_139694.2 |
CG4603-RA (CG4603), mRNA |
| 0.2 |
1 |
2 |
3 |
4 |
NM_135184.2 |
CG9528-RA (retm), mRNA |
| 0 |
0 |
5 |
13 |
24 |
NM_167647.2 |
CG12199-RB, transcript variant B (kek5), mRNA |
| 0 |
0 |
5 |
13 |
24 |
NM_133154.2 |
CG12199-RA, transcript variant A (kek5), mRNA |
| 0 |
0 |
4 |
10 |
36 |
NM_080531.2 |
CG5481-RA (lea), mRNA |
| 0 |
0 |
4 |
9 |
14 |
NM_078740.2 |
CG8817-RB, transcript variant B (lilli), mRNA |
| 0 |
0 |
4 |
9 |
14 |
NM_164516.1 |
CG8817-RA, transcript variant A (lilli), mRNA |
| 0 |
0 |
4 |
9 |
14 |
NM_164517.1 |
CG8817-RC, transcript variant C (lilli), mRNA |
| 0 |
0 |
4 |
7 |
10 |
NM_136649.1 |
CG12932-RA (CG12932), mRNA |
| 0 |
0 |
4 |
3 |
6 |
NM_001014453.1 |
CG33526-RD, transcript variant D (PNUTS), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_001014452.1 |
CG33526-RC, transcript variant C (PNUTS), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_001014455.1 |
CG33526-RB, transcript variant B (PNUTS), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_001014454.1 |
CG33526-RA, transcript variant A (PNUTS), mRNA |
| 0 |
0 |
4 |
2 |
6 |
NM_057792.2 |
CG9019-RA (dsf), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_136290.2 |
CG6448-RB, transcript variant B (CG6448), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_165411.1 |
CG6448-RA, transcript variant A (CG6448), mRNA |
| 0 |
0 |
3 |
20 |
77 |
NM_058124.2 |
CG5772-RA (Sur), mRNA |
| 0 |
0 |
3 |
9 |
16 |
NM_176723.2 |
CG33175-RG, transcript variant G (spri), mRNA |
| 0 |
0 |
3 |
9 |
16 |
NM_206678.1 |
CG33175-RA, transcript variant A (spri), mRNA |
| 0 |
0 |
3 |
8 |
14 |
NM_165490.1 |
CG3427-RA (Epac), mRNA |
| 0 |
0 |
3 |
8 |
9 |
NM_079356.2 |
CG18492-RA (Tak1), mRNA |
| 0 |
0 |
3 |
6 |
18 |
NM_080306.2 |
CG3443-RB (pcx), mRNA |
| 0 |
0 |
3 |
4 |
18 |
NM_143001.1 |
CG17781-RA (CG17781), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_134874.2 |
CG2973-RA (CG2973), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_143200.1 |
CG14549-RA (Sld5), mRNA |
| 0 |
0 |
3 |
2 |
1 |
NM_170347.1 |
CG31055-RC, transcript variant C (CG31055), mRNA |
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