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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4353R-2 
 Symbol hep  Full Name hemipterous 
 CG No CG4353  Old CG No CG4353 
 Synonyms HEP/MKK7, DJNKK, HEP, JNKK, MKK7, DHEP/MKK7, CG2190, CG4353, DMKK7, hp, l(1)G0107, l(1)G0208, l(1)7P1, hem, hep, Hep 
 Accession No (Link to NCBI) NM_167346.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Clavier A, Ruby V, Rincheval-Arnold A, Mignotte B, Guénal I.
The Drosophila retinoblastoma protein, Rbf1, induces a Debcl- and Drp1-dependent mitochondrial apoptosis.
J. Cell. Sci. (2015) 128(17) 3239-49 [ PubMed ID = 26208635 ] [ RRC reference ]

Demay Y, Perochon J, Szuplewski S, Mignotte B, Gaumer S.
The PERK pathway independently triggers apoptosis and a Rac1/Slpr/JNK/Dilp8 signaling favoring tissue homeostasis in a chronic ER stress Drosophila model.
Cell Death Dis (2014) 5 e1452 [ PubMed ID = 25299777 ] [ RRC reference ]

Clavier A, Rincheval-Arnold A, Baillet A, Mignotte B, Guénal I.
Two different specific JNK activators are required to trigger apoptosis or compensatory proliferation in response to Rbf1 in Drosophila.
Cell Cycle (2016) 15(2) 283-94 [ PubMed ID = 26825229 ] [ RRC reference ]

Marchal C, Vinatier G, Sanial M, Plessis A, Pret AM, Limbourg-Bouchon B, Théodore L, Netter S.
The HIV-1 Vpu protein induces apoptosis in Drosophila via activation of JNK signaling.
PLoS ONE (2012) 7(3) e34310 [ PubMed ID = 22479597 ] [ RRC reference ]

Neisch AL, Speck O, Stronach B, Fehon RG.
Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
J. Cell Biol. (2010) 189(2) 311-23 [ PubMed ID = 20404112 ] [ RRC reference ]

Ishimaru S, Ueda R, Hinohara Y, Ohtani M, Hanafusa H.
PVR plays a critical role via JNK activation in thorax closure during Drosophila metamorphosis.
EMBO J. (2004) 23(20) 3984-94 [ PubMed ID = 15457211 ] [ RRC reference ]

Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57 [ PubMed ID = 20813879 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgACCTTCCTTGGGCGCAGGTTC 
 Primer Seq. 3'
aatctagaggtaccCAGTGCGTCGCATCTGCTTCA 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 ACCTTCCTTG GGCGCAGGTT CCGTTTCCGG ATCGGGTATC AGCATAGCCC AGCGTCCAGN 
0061 ACACCTCCAG TTCCTCATTG CGACNCCCTT CGGCAGCGCC AGCGCCTCGT CATCATCCTC 
0121 ATCCGCATCC GCATTTGCAT CCGCAGCACC GGCGACGNGC ACCTNTCGGT GGAACATACA 
0181 CCCCGCCGAC AACTAGAGTG TCGCGAGCCA CGCCCACACT GCCCATGCTT TCTAGTGGTC 
0241 CCGGTGGTGG ACTGAATCGT ACGCGGCCGG TGATACTTCC GCTGCCCACG CCGCCACATC 
0301 CTCCAGTTTC GGAAACGGAC ATGAAGCTGA AGATCATCAT GGAGCAGACC GGCAAGCTGA 
0361 ACATCAACGG GCGGCAGTAT CCGACGGACA TCAATGACCT CAAGCACCTG GGCGACCTGG 
0421 GCAATGGGAC TAGCGGCAAT GTGGTGAAGA TGATGCACCT GTCCAGCAAC ACGATCATCG 
0481 CCGTGAAGCA GATgNGACGC ACTG 
 in silico PCR Fragment
0001 ACCTTCCTTG GGCGCAGGTT CCGTTTCCGG ATCGGGTATC AGCATAGCCC AGCGTCCAG- 
0061 -CACCTCCAG TTCCTCA-TG CGACGCCCTT CGGCAGCGCC AGCGCCTCGT CATCATCCTC 
0121 ATCCGCATCC GCATTTGCAT CCGCAGCACC GGCGACGGGC ACCT-TCGGT GGAACATACA 
0181 CCCCGCCGAC AACTAGAGTG TCGCGAGCCA CGCCCACACT GCCCATGCTT TCTAGTGGTC 
0241 CCGGTGGTGG ACTGAATCGT ACGCGGCCGG TGATACTTCC GCTGCCCACG CCGCCACATC 
0301 CTCCAGTTTC GGAAACGGAC ATGAAGCTGA AGATCATCAT GGAGCAGACC GGCAAGCTGA 
0361 ACATCAACGG GCGGCAGTAT CCGACGGACA TCAATGACCT CAAGCACCTG GGCGACCTGG 
0421 GCAATGGGAC TAGCGGCAAT GTGGTGAAGA TGATGCACCT GTCCAGCAAC ACGATCATCG 
0481 CCGTGAAGCA GATGCGACGC ACTG 
 Assemble Data

4353R-2.IR_full       1   ACCTTCCTTGGGCGCAGGTTCCGTTTCCGGATCGGGTATCAGCATAGCCCAGCGTCCAGN 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
4353R-2.in silico     1   ACCTTCCTTGGGCGCAGGTTCCGTTTCCGGATCGGGTATCAGCATAGCCCAGCGTCCAG- 60


4353R-2.IR_full       61  ACACCTCCAGTTCCTCATTGCGACNCCCTTCGGCAGCGCCAGCGCCTCGTCATCATCCTC 120
                           |||||||||||||||| |||||| |||||||||||||||||||||||||||||||||||
4353R-2.in silico     61  -CACCTCCAGTTCCTCA-TGCGACGCCCTTCGGCAGCGCCAGCGCCTCGTCATCATCCTC 120


4353R-2.IR_full       121 ATCCGCATCCGCATTTGCATCCGCAGCACCGGCGACGNGCACCTNTCGGTGGAACATACA 180
                          ||||||||||||||||||||||||||||||||||||| |||||| |||||||||||||||
4353R-2.in silico     121 ATCCGCATCCGCATTTGCATCCGCAGCACCGGCGACGGGCACCT-TCGGTGGAACATACA 180


4353R-2.IR_full       181 CCCCGCCGACAACTAGAGTGTCGCGAGCCACGCCCACACTGCCCATGCTTTCTAGTGGTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4353R-2.in silico     181 CCCCGCCGACAACTAGAGTGTCGCGAGCCACGCCCACACTGCCCATGCTTTCTAGTGGTC 240


4353R-2.IR_full       241 CCGGTGGTGGACTGAATCGTACGCGGCCGGTGATACTTCCGCTGCCCACGCCGCCACATC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4353R-2.in silico     241 CCGGTGGTGGACTGAATCGTACGCGGCCGGTGATACTTCCGCTGCCCACGCCGCCACATC 300


4353R-2.IR_full       301 CTCCAGTTTCGGAAACGGACATGAAGCTGAAGATCATCATGGAGCAGACCGGCAAGCTGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4353R-2.in silico     301 CTCCAGTTTCGGAAACGGACATGAAGCTGAAGATCATCATGGAGCAGACCGGCAAGCTGA 360


4353R-2.IR_full       361 ACATCAACGGGCGGCAGTATCCGACGGACATCAATGACCTCAAGCACCTGGGCGACCTGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4353R-2.in silico     361 ACATCAACGGGCGGCAGTATCCGACGGACATCAATGACCTCAAGCACCTGGGCGACCTGG 420


4353R-2.IR_full       421 GCAATGGGACTAGCGGCAATGTGGTGAAGATGATGCACCTGTCCAGCAACACGATCATCG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4353R-2.in silico     421 GCAATGGGACTAGCGGCAATGTGGTGAAGATGATGCACCTGTCCAGCAACACGATCATCG 480


4353R-2.IR_full       481 CCGTGAAGCAGATGNGACGCACTG 504
                          |||||||||||||| |||||||||
4353R-2.in silico     481 CCGTGAAGCAGATGCGACGCACTG 504

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  26  NM_167346.1  CG4353-RA, transcript variant A (hep), mRNA 
100   482  24  NM_078587.2  CG4353-RC, transcript variant C (hep), mRNA 
0.62   34  NM_001032002.1  CG33676-RA, transcript variant A (Skeletor), mRNA 
0.62   34  NM_001032000.1  CG14681-RB, transcript variant B (CG14681), mRNA 
0.2   NM_139694.2  CG4603-RA (CG4603), mRNA 
0.2   NM_135184.2  CG9528-RA (retm), mRNA 
0   13  24  NM_167647.2  CG12199-RB, transcript variant B (kek5), mRNA 
0   13  24  NM_133154.2  CG12199-RA, transcript variant A (kek5), mRNA 
0   10  36  NM_080531.2  CG5481-RA (lea), mRNA 
0   14  NM_078740.2  CG8817-RB, transcript variant B (lilli), mRNA 
0   14  NM_164516.1  CG8817-RA, transcript variant A (lilli), mRNA 
0   14  NM_164517.1  CG8817-RC, transcript variant C (lilli), mRNA 
0   10  NM_136649.1  CG12932-RA (CG12932), mRNA 
0   NM_001014453.1  CG33526-RD, transcript variant D (PNUTS), mRNA 
0   NM_001014452.1  CG33526-RC, transcript variant C (PNUTS), mRNA 
0   NM_001014455.1  CG33526-RB, transcript variant B (PNUTS), mRNA 
0   NM_001014454.1  CG33526-RA, transcript variant A (PNUTS), mRNA 
0   NM_057792.2  CG9019-RA (dsf), mRNA 
0   NM_136290.2  CG6448-RB, transcript variant B (CG6448), mRNA 
0   NM_165411.1  CG6448-RA, transcript variant A (CG6448), mRNA 
0   20  77  NM_058124.2  CG5772-RA (Sur), mRNA 
0   16  NM_176723.2  CG33175-RG, transcript variant G (spri), mRNA 
0   16  NM_206678.1  CG33175-RA, transcript variant A (spri), mRNA 
0   14  NM_165490.1  CG3427-RA (Epac), mRNA 
0   NM_079356.2  CG18492-RA (Tak1), mRNA 
0   18  NM_080306.2  CG3443-RB (pcx), mRNA 
0   18  NM_143001.1  CG17781-RA (CG17781), mRNA 
0   NM_134874.2  CG2973-RA (CG2973), mRNA 
0   NM_143200.1  CG14549-RA (Sld5), mRNA 
0   NM_170347.1  CG31055-RC, transcript variant C (CG31055), mRNA 

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