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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4322R-1 |
| Symbol |
moody |
Full Name |
moody |
| CG No |
CG4322 |
Old CG No |
CG4322 |
| Synonyms |
1631, EG:22E5.11, EP1631, unnamed, moody, CG4322 |
| Accession No (Link to NCBI) |
NM_130614.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Xu C, Franklin B, Tang HW, Regimbald-Dumas Y, Hu Y, Ramos J, Bosch JA, Villalta C, He X, Perrimon N.
An in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity.
Proc Natl Acad Sci U S A (2020) 117(1) 464-471
[
PubMed ID = 31852821
]
[
RRC reference
]
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTGGCTGTTCTCCTACGGCAT |
| Primer Seq. 3' |
aatctagaggtaccGGCCAGCACCATCTTAGTGAT |
| Predicted Fragment Size |
472 |
| IR fragment full Seq |
0001 NTGGCTGTTC TCCTACGGCA TGCAGCTCCC GACGCTTCTG GGAGAGTGGG GTCGCTTTGG
0061 CTACGACTCG AGATTACAGA CCTGCTCCAT CATGACCGAC GACCATGGGC ACAGCAGCAA
0121 GACGACGTTG TTTATCACCG CCTTCGTCAT CCCTTGCCTA GTTATAATTG CCTGCTATGC
0181 CAAGATCTTC TGGGTGGTCC ACAAGTCGGA GCAGCGCTTG AAGCGCCATG CGACCAATAC
0241 AAAACTCCAT ACCCAACAAC CTGCGCCCCC TAGCCTCTAC AGGATCAGGA GCCCTGCCCT
0301 CCGGCGCGGA ATGCCAGCCA AGCAACCGCG TCTCCTCGGA CAGCAGCAGT AGCTTCTCTC
0361 ATCGATGTGC CGGAGACAGC GCCCAGCGGC TAAGCAGCAG CCAACCCGAG TCAAGGATCA
0421 GCGTGAAGTA CGTGCCAAGC GAAACGAGTG GCGCATCACT AAGATGGTGC TGGCC |
| in silico PCR Fragment |
0001 CTGGCTGTTC TCCTACGGCA TGCAGCTCCC GACGCTTCTG GGAGAGTGGG GTCGCTTTGG
0061 CTACGACTCG AGATTACAGA CCTGCTCCAT CATGACCGAC GACCATGGGC ACAGCAGCAA
0121 GACGACGTTG TTTATCACCG CCTTCGTCAT CCCTTGCCTG GTTATCATTG CCTGCTATGC
0181 CAAGATCTTC TGGGTGGTCC ACAAGTCGGA GCAGCGCTTG AAGCGCCATG CCACCAA-AC
0241 AAAACTCCAT ACCCAACAAC CTCCGCCCCC TAGCCTCTAC AGGATCAGGA GCCCTGCCCT
0301 CCGGCGCGGA ATGCCAGCCA AGCAACCGCG TCTCCTCGGA CAGCAGCAGT AGCTTCTC-C
0361 ATCGATGTGC CGGAGACAGC GCCCAGCGGC -AAGCAGCAG CCAACCCGAG TCAAGGATCA
0421 GCGTGAAGTA CGTGCCAAGC GAAACGAGTG GCGCATCACT AAGATGGTGC TGGCC |
| Assemble Data |
4322R-1.IR_full 1 TGGCTGTTCTCCTACGGCATGCAGCTCCCGACGCTTCTGGGAGAGTGGGGTCGCTTTGG 59
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4322R-1.in silico 1 TGGCTGTTCTCCTACGGCATGCAGCTCCCGACGCTTCTGGGAGAGTGGGGTCGCTTTGG 59
4322R-1.IR_full 61 CTACGACTCGAGATTACAGACCTGCTCCATCATGACCGACGACCATGGGCACAGCAGCAA 119
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4322R-1.in silico 61 CTACGACTCGAGATTACAGACCTGCTCCATCATGACCGACGACCATGGGCACAGCAGCAA 119
4322R-1.IR_full 121 GACGACGTTGTTTATCACCGCCTTCGTCATCCCTTGCCTAGTTATAATTGCCTGCTATGC 179
||||||||||||||||||||||||||||||||||||||| ||||| ||||||||||||||
4322R-1.in silico 121 GACGACGTTGTTTATCACCGCCTTCGTCATCCCTTGCCTGGTTATCATTGCCTGCTATGC 179
4322R-1.IR_full 181 CAAGATCTTCTGGGTGGTCCACAAGTCGGAGCAGCGCTTGAAGCGCCATGCGACCAATAC 239
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| ||
4322R-1.in silico 181 CAAGATCTTCTGGGTGGTCCACAAGTCGGAGCAGCGCTTGAAGCGCCATGCCACCAA-AC 239
4322R-1.IR_full 241 AAAACTCCATACCCAACAACCTGCGCCCCCTAGCCTCTACAGGATCAGGAGCCCTGCCCT 299
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4322R-1.in silico 241 AAAACTCCATACCCAACAACCTCCGCCCCCTAGCCTCTACAGGATCAGGAGCCCTGCCCT 299
4322R-1.IR_full 301 CCGGCGCGGAATGCCAGCCAAGCAACCGCGTCTCCTCGGACAGCAGCAGTAGCTTCTCTC 359
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
4322R-1.in silico 301 CCGGCGCGGAATGCCAGCCAAGCAACCGCGTCTCCTCGGACAGCAGCAGTAGCTTCTC-C 359
4322R-1.IR_full 361 ATCGATGTGCCGGAGACAGCGCCCAGCGGCTAAGCAGCAGCCAACCCGAGTCAAGGATCA 419
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4322R-1.in silico 361 ATCGATGTGCCGGAGACAGCGCCCAGCGGC-AAGCAGCAGCCAACCCGAGTCAAGGATCA 419
4322R-1.IR_full 421 GCGTGAAGTACGTGCCAAGCGAAACGAGTGGCGCATCACTAAGATGGTGCTGGCC 474
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4322R-1.in silico 421 GCGTGAAGTACGTGCCAAGCGAAACGAGTGGCGCATCACTAAGATGGTGCTGGCC 474
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
454 |
0 |
0 |
0 |
NM_130614.2 |
CG4322-RA (CG4322), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_079665.2 |
CG7623-RA (sll), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_001043123.1 |
CG32387-RC, transcript variant C (CG32387), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_168195.1 |
CG32387-RB, transcript variant B (CG32387), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_176181.1 |
CG8291-RC, transcript variant C (CG8291), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_137220.2 |
CG8291-RA, transcript variant A (CG8291), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_166121.2 |
CG8291-RB, transcript variant B (CG8291), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080516.1 |
CG16757-RA (Spn), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_138026.3 |
CG3907-RB, transcript variant B (CG3907), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_166647.2 |
CG3907-RA, transcript variant A (CG3907), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_168470.1 |
CG32085-RA (CG32085), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_079017.2 |
CG8542-RA (Hsc70-5), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_137819.2 |
CG11206-RA, transcript variant A (CG11206), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_206200.1 |
CG11206-RB, transcript variant B (CG11206), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_176108.1 |
CG8080-RA (CG8080), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079694.2 |
CG5460-RC, transcript variant C (H), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169907.1 |
CG5460-RA, transcript variant A (H), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169908.1 |
CG5460-RD, transcript variant D (H), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169909.1 |
CG5460-RB, transcript variant B (H), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167279.1 |
CG18361-RB, transcript variant B (dsh), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_078563.2 |
CG18361-RA, transcript variant A (dsh), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137032.2 |
CG5970-RA (CG5970), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001038794.1 |
CG34010-RA (CG34010), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_176435.1 |
CG8176-RA, transcript variant A (CG8176), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_176436.1 |
CG8176-RB, transcript variant B (CG8176), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_136154.1 |
CG10631-RA (CG10631), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001014540.1 |
CG8201-RN, transcript variant N (par-1), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001014542.1 |
CG8201-RL, transcript variant L (par-1), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_206178.1 |
CG8201-RA, transcript variant A (par-1), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_206177.1 |
CG8201-RB, transcript variant B (par-1), mRNA |
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