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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4155R-1 |
| Symbol |
Brf |
Full Name |
Brf |
| CG No |
CG31256 |
Old CG No |
CG4155 |
| Synonyms |
TFIIIB, BRF, BRF1, CG5419, CG4155, CG31256, Brf |
| Accession No (Link to NCBI) |
NM_142359.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Sriskanthadevan-Pirahas S, Deshpande R, Lee B, Grewal SS.
Ras/ERK-signalling promotes tRNA synthesis and growth via the RNA polymerase III repressor Maf1 in Drosophila.
PLoS Genet (2018) 14(2) e1007202
[
PubMed ID = 29401457
]
[
RRC reference
]
Marshall L, Rideout EJ, Grewal SS.
Nutrient/TOR-dependent regulation of RNA polymerase III controls tissue and organismal growth in Drosophila.
EMBO J (2012) 31(8) 1916-30
[
PubMed ID = 22367393
]
[
RRC reference
]
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93
[
PubMed ID = 26015542
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Kamimura K, Koyama T, Habuchi H, Ueda R, Masu M, Kimata K, Nakato H.
Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling.
J Cell Biol (2006) 174(6) 773-8
[
PubMed ID = 16966419
]
[
RRC reference
]
Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.
Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.
Proc Natl Acad Sci U S A (2006) 103(39) 14417-22
[
PubMed ID = 16971484
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGCCAACCGACTGCAGTTGGG |
| Primer Seq. 3' |
aatctagaggtaccTGCTATTGGGCGTGCTTGGAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGCCAACCGA CTGCAGTTGG GTGCCAAAAC GCACGAGGTT TCGATGACGG CTCTGAGGAT
0061 TGTCCAGCGC ATGAAAAAGG ACTGCATGCA CTCGGGACGA CGACCCACTG GCCTTTGTGG
0121 AGCAGCTTTG CTGATTGCGG CCCGGATGCA CGATTTTAGT CGCACCATGC TGGACGTGAT
0181 AGGTGTGGTC AACGATACAC GAGTCAACGC TCCGAAAGCG TCTTTCGGAG TTTGCTGAAA
0241 CGCCCTCGGG AGGGCTAACC CTGGAGGAGT TCATGACCGT TGACTTGGAG CGCGAACAGG
0301 ATCCGCCTTC CTTTATAGGC AGCACCGGCA AAAAGGATCG TGAGAGCAAT CAAAGATATG
0361 GGCGAGCATG AGCTAACAGA ATTGCAAAAA GAAATTGATG CTCATCTGGA AAAAGATCTA
0421 GGCAAGTACT CaAACTCCGT TTACCGTCAG CTGACTAAGG GTAAGGGATT AAGTCCACTG
0481 AGCTCTCCAA GCACGCCCAA TAGCA |
| in silico PCR Fragment |
0001 TGCCAACCGA CTGCAGTTGG GTGCCAAAAC GCACGAGGTT TCGATGACGG CTCTGAGGAT
0061 TGTCCAGCGC ATGAAAAAGG ACTGCATGCA CTCGGGACGA CGACCCACTG GCCTTTGTGG
0121 AGCAGCTTTG CTGATTGCGG CCCGGATGCA CGATTTTAGT CGCACCATGC TGGACGTGAT
0181 AGGTGTGGTC AA-GATACAC GAGTCAACGC TCCGAAAGCG TCTTTCGGAG TTTGCTGAAA
0241 CGCCCTCGGG AGGGCTAACC CTGGAGGAGT TCATGACCGT TGACTTGGAG CGCGAACAGG
0301 ATCCGCCTTC CTTTA-AGGC AGCAC--GCA AAAAGGATCG TGAGAG-AAT CAAAGATATG
0361 GGCGAGCATG AGCTAACAGA ATTGCAAAAA GAAATTGATG CTCATCTGGA AAAAGATCTA
0421 GGCAAGTACT CAAACTCCGT TTACCGTCAG CTGACTAAGG GTAAGGGATT AAGTCCACTG
0481 AGCTCTCCAA GCACGCCCAA TAGCA |
| Assemble Data |
4155R-1.IR_full 1 TGCCAACCGACTGCAGTTGGGTGCCAAAACGCACGAGGTTTCGATGACGGCTCTGAGGAT 60
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4155R-1.in silico 1 TGCCAACCGACTGCAGTTGGGTGCCAAAACGCACGAGGTTTCGATGACGGCTCTGAGGAT 60
4155R-1.IR_full 61 TGTCCAGCGCATGAAAAAGGACTGCATGCACTCGGGACGACGACCCACTGGCCTTTGTGG 120
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4155R-1.in silico 61 TGTCCAGCGCATGAAAAAGGACTGCATGCACTCGGGACGACGACCCACTGGCCTTTGTGG 120
4155R-1.IR_full 121 AGCAGCTTTGCTGATTGCGGCCCGGATGCACGATTTTAGTCGCACCATGCTGGACGTGAT 180
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4155R-1.in silico 121 AGCAGCTTTGCTGATTGCGGCCCGGATGCACGATTTTAGTCGCACCATGCTGGACGTGAT 180
4155R-1.IR_full 181 AGGTGTGGTCAACGATACACGAGTCAACGCTCCGAAAGCGTCTTTCGGAGTTTGCTGAAA 240
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4155R-1.in silico 181 AGGTGTGGTCAA-GATACACGAGTCAACGCTCCGAAAGCGTCTTTCGGAGTTTGCTGAAA 240
4155R-1.IR_full 241 CGCCCTCGGGAGGGCTAACCCTGGAGGAGTTCATGACCGTTGACTTGGAGCGCGAACAGG 300
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4155R-1.in silico 241 CGCCCTCGGGAGGGCTAACCCTGGAGGAGTTCATGACCGTTGACTTGGAGCGCGAACAGG 300
4155R-1.IR_full 301 ATCCGCCTTCCTTTATAGGCAGCACCGGCAAAAAGGATCGTGAGAGCAATCAAAGATATG 360
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4155R-1.in silico 301 ATCCGCCTTCCTTTA-AGGCAGCAC--GCAAAAAGGATCGTGAGAG-AATCAAAGATATG 360
4155R-1.IR_full 361 GGCGAGCATGAGCTAACAGAATTGCAAAAAGAAATTGATGCTCATCTGGAAAAAGATCTA 420
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4155R-1.in silico 361 GGCGAGCATGAGCTAACAGAATTGCAAAAAGAAATTGATGCTCATCTGGAAAAAGATCTA 420
4155R-1.IR_full 421 GGCAAGTACTCAAACTCCGTTTACCGTCAGCTGACTAAGGGTAAGGGATTAAGTCCACTG 480
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4155R-1.in silico 421 GGCAAGTACTCAAACTCCGTTTACCGTCAGCTGACTAAGGGTAAGGGATTAAGTCCACTG 480
4155R-1.IR_full 481 AGCTCTCCAAGCACGCCCAATAGCA 505
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4155R-1.in silico 481 AGCTCTCCAAGCACGCCCAATAGCA 505
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
12 |
NM_142359.2 |
CG31256-RA (Brf), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_135360.3 |
CG7830-RA (CG7830), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_164683.1 |
CG31641-RB, transcript variant B (stai), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_164682.1 |
CG31641-RC, transcript variant C (stai), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_057784.4 |
CG7833-RA (Orc5), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_176177.1 |
CG33139-RA (Ranbp11), mRNA |
| 0 |
0 |
1 |
4 |
34 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_134490.2 |
CG12238-RA (l(1)G0084), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_139990.2 |
CG5971-RA (CG5971), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_131921.1 |
CG15375-RA (CG15375), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_136455.1 |
CG2093-RA (CG2093), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001038780.1 |
CG33979-RA, transcript variant A (capt), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001038781.1 |
CG33979-RB, transcript variant B (capt), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_139443.1 |
CG13809-RA (osm-1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079232.1 |
CG14827-RA (mei-P22), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168471.1 |
CG11652-RB, transcript variant B (CG11652), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080018.1 |
CG6890-RA (Tollo), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078507.2 |
CG3926-RA (Spat), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_164549.1 |
CG31778-RA (CG31778), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_134654.1 |
CG4648-RA (CG4648), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_132125.1 |
CG4536-RA (CG4536), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169502.1 |
CG8774-RB, transcript variant B (CG8774), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142017.2 |
CG8774-RA, transcript variant A (CG8774), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_057548.3 |
CG10385-RA (msl-1), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_001032402.1 |
CG33957-RB, transcript variant B (cp309), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_168258.1 |
CG7404-RA, transcript variant A (ERR), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_139926.1 |
CG7404-RB, transcript variant B (ERR), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_139460.1 |
CG9004-RA (CG9004), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_001014694.1 |
CG1464-RC, transcript variant C (ey), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_079889.2 |
CG1464-RA, transcript variant A (ey), mRNA |
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