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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4063R-2 |
| Symbol |
ebi |
Full Name |
ebi |
| CG No |
CG4063 |
Old CG No |
CG4063 |
| Synonyms |
Ebi, Tbl1, Tb11, l(2)k16213, CG4063, ebi |
| Accession No (Link to NCBI) |
NM_057981.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Nguyen MB, Vuong LT, Choi KW.<br> Ebi modulates wing growth by ubiquitin-dependent downregulation of Crumbs in Drosophila.<br> Development (2016) 143(19) 3506-3513
[
PubMed ID = 27702784
]
[
RRC reference
]
Dui W, Lu W, Ma J, Jiao R.<br> A systematic phenotypic screen of F-box genes through a tissue-specific RNAi-based approach in Drosophila.<br> J Genet Genomics (2012) 39(8) 397-413
[
PubMed ID = 22884096
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGGGCTCAGCAGGTGGAAACA |
| Primer Seq. 3' |
aatctagaggtaccTCCTTGGGCTCCTACGCGAAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGGGCTCAGC AGGTGGAAAC AAAATTGCTG GCAGCACCAC GGGCACAAGC ACACCAACCG
0061 ATCAGTCGGC AAGCGAGGTA GACTCCAGTG GAAATGCGGC AAATAATGCT GGCGGCACAT
0121 ACGCCGGNAA ACAATGGAGC TGGAGGGAAC CAGGCCAGTA CTGGAGGATC TAATAGCACA
0181 AGCACACCAG CTGGTGGAGA TTTGGCAGCA CCTGGGGCAA GTCAAAAGAA ATCCCAGAAC
0241 TCGAATGAAG CGGGAAGCTC ATCTAGTGGC AATGCAGGCA ATGCAAATGC CACCAGTACC
0301 GATGATGCAG CCTCCTCAAC ATCCACAAAT GGCAATAGCA GCACTTCCAG CAGCGTAGAA
0361 CAACCTacaa gcgNNCTAGN CGCNCGCTGG TGGAACTGTA TCAACCTCCA ATCCGGACGC
0421 AGCAGCATCG GGCGGAGCTT CGACTGCAAC AGGATCAAAG GCGCCAAGTG GAGCGGTTAC
0481 GATTCGCGTA GGAGCCCAAG GA |
| in silico PCR Fragment |
0001 CGGGCTCAGC AGGTGGAAAC AAAATTGCTG GCAGCACCAC GGGCACAAGC ACACCAACCG
0061 ATCAGTCGGC AAGCGAGGTA GACTCCAGTG GAAATGCGGC AAATAATGCT GGCGGCACAT
0121 ACGCCGG-AA ACAATGGAGC TGGAGGGAAC CAGGCCAGTA CTGGAGGATC TAATAGCACA
0181 AGCACACCAG CTGGTGGAGA TTTGGCAGCA CCTGGGGCAA GTCAAAAGAA ATCCCAGAAC
0241 TCGAATGAAG CGGGAAGCTC ATCTAGTGGC AATGCAGGCA ATGCAAATGC TACCAGTACC
0301 GATGATGCAG CCTCCTCAAC ATCCACAAAT GGCAATAGCA GCACTTCCAG CAGCGTAGAA
0361 CAACCTACAA GCGGGCT-GA CGCCCGCTGG TGGAACTGTA TCAACCTCCA ATCCGGACGC
0421 AGCAGCATCG GGCGGAGCTT CGACTGCAAC AGGATCAAAG GCGCCAAGTG GAGCGGTTAC
0481 GATTCGCGTA GGAGCCCAAG GA |
| Assemble Data |
<PRE>
4063R-2.IR_full 1 CGGGCTCAGCAGGTGGAAACAAAATTGCTGGCAGCACCACGGGCACAAGCACACCAACCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4063R-2.in silico 1 CGGGCTCAGCAGGTGGAAACAAAATTGCTGGCAGCACCACGGGCACAAGCACACCAACCG 60
4063R-2.IR_full 61 ATCAGTCGGCAAGCGAGGTAGACTCCAGTGGAAATGCGGCAAATAATGCTGGCGGCACAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4063R-2.in silico 61 ATCAGTCGGCAAGCGAGGTAGACTCCAGTGGAAATGCGGCAAATAATGCTGGCGGCACAT 120
4063R-2.IR_full 121 ACGCCGG<span class="snp"><tt>N</tt></span>AAACAATGGAGCTGGAGGGAACCAGGCCAGTACTGGAGGATCTAATAGCACA 180
|||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
4063R-2.in silico 121 ACGCCGG<span class="snp"><tt>-</tt></span>AAACAATGGAGCTGGAGGGAACCAGGCCAGTACTGGAGGATCTAATAGCACA 180
4063R-2.IR_full 181 AGCACACCAGCTGGTGGAGATTTGGCAGCACCTGGGGCAAGTCAAAAGAAATCCCAGAAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4063R-2.in silico 181 AGCACACCAGCTGGTGGAGATTTGGCAGCACCTGGGGCAAGTCAAAAGAAATCCCAGAAC 240
4063R-2.IR_full 241 TCGAATGAAGCGGGAAGCTCATCTAGTGGCAATGCAGGCAATGCAAATGC<span class="snp"><tt>C</tt></span>ACCAGTACC 300
||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||
4063R-2.in silico 241 TCGAATGAAGCGGGAAGCTCATCTAGTGGCAATGCAGGCAATGCAAATGC<span class="snp"><tt>T</tt></span>ACCAGTACC 300
4063R-2.IR_full 301 GATGATGCAGCCTCCTCAACATCCACAAATGGCAATAGCAGCACTTCCAGCAGCGTAGAA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4063R-2.in silico 301 GATGATGCAGCCTCCTCAACATCCACAAATGGCAATAGCAGCACTTCCAGCAGCGTAGAA 360
4063R-2.IR_full 361 CAACCTACAAGCG<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>CT<span class="snp"><tt>A</tt></span>G<span class="snp"><tt>N</tt></span>CGC<span class="snp"><tt>N</tt></span>CGCTGGTGGAACTGTATCAACCTCCAATCCGGACGC 420
|||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>|||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||
4063R-2.in silico 361 CAACCTACAAGCG<span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span>CT<span class="snp"><tt>-</tt></span>G<span class="snp"><tt>A</tt></span>CGC<span class="snp"><tt>C</tt></span>CGCTGGTGGAACTGTATCAACCTCCAATCCGGACGC 420
4063R-2.IR_full 421 AGCAGCATCGGGCGGAGCTTCGACTGCAACAGGATCAAAGGCGCCAAGTGGAGCGGTTAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4063R-2.in silico 421 AGCAGCATCGGGCGGAGCTTCGACTGCAACAGGATCAAAGGCGCCAAGTGGAGCGGTTAC 480
4063R-2.IR_full 481 GATTCGCGTAGGAGCCCAAGGA 502
||||||||||||||||||||||
4063R-2.in silico 481 GATTCGCGTAGGAGCCCAAGGA 502
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
6 |
NM_057981.3 |
CG4063-RA (ebi), mRNA |
| 0 |
0 |
3 |
5 |
9 |
NM_168179.1 |
CG32394-RA (CG32394), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_168551.1 |
CG32130-RA, transcript variant A (stv), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_079336.2 |
CG7002-RA (Hml), mRNA |
| 0 |
0 |
1 |
7 |
9 |
NM_143372.1 |
CG9995-RA (htt), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_139917.2 |
CG7927-RA (CG7927), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_175945.1 |
CG4297-RB, transcript variant B (CG4297), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_134683.1 |
CG4297-RA, transcript variant A (CG4297), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_169964.1 |
CG31343-RA (CG31343), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_132097.1 |
CG3950-RA (CG3950), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167662.1 |
CG3917-RA, transcript variant A (Grip84), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136375.1 |
CG3273-RA (sced), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_078685.2 |
CG3917-RC, transcript variant C (Grip84), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167663.1 |
CG3917-RB, transcript variant B (Grip84), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141240.1 |
CG14656-RA (CG14656), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141444.1 |
CG10061-RA (l(3)s2214), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_136151.1 |
CG10366-RA (CG10366), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_137056.2 |
CG13340-RA (CG13340), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_135238.2 |
CG10354-RA (CG10354), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141501.1 |
CG3223-RA (CG3223), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079395.2 |
CG9695-RA (Dab), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168157.1 |
CG12755-RB, transcript variant B (l(3)mbn), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001014570.1 |
CG12755-RC, transcript variant C (l(3)mbn), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134868.1 |
CG15399-RA (CG15399), mRNA |
| 0 |
0 |
0 |
11 |
36 |
NM_206332.1 |
CG18331-RA (CG18331), mRNA |
| 0 |
0 |
0 |
9 |
13 |
NM_139493.2 |
CG2083-RA (CG2083), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_168881.1 |
CG32434-RB, transcript variant B (siz), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_001043153.1 |
CG32434-RC, transcript variant C (siz), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_206407.1 |
CG32434-RA, transcript variant A (siz), mRNA |
| 0 |
0 |
0 |
5 |
16 |
NM_131975.3 |
CG32767-RA, transcript variant A (CG32767), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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