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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4012R-2 |
| Symbol |
gek |
Full Name |
genghis khan |
| CG No |
CG4012 |
Old CG No |
CG4012 |
| Synonyms |
MRCKalpha, CG4012, dMRCK, l(2)09373, Gek, anon-WO0200864.1, Mrck, MdaPk, gek |
| Accession No (Link to NCBI) |
NM_079113.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ostalé CM, Esteban N, López-Varea A, de Celis JF.<br> Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.<br> G3 (Bethesda) (2021) 11(12)
[
PubMed ID = 34599799
]
[
RRC reference
]
Read RD, Fenton TR, Gomez GG, Wykosky J, Vandenberg SR, Babic I, Iwanami A, Yang H, Cavenee WK, Mischel PS, Furnari FB, Thomas JB.<br> A kinome-wide RNAi screen in Drosophila Glia reveals that the RIO kinases mediate cell proliferation and survival through TORC2-Akt signaling in glioblastoma.<br> PLoS Genet (2013) 9(2) e1003253
[
PubMed ID = 23459592
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAGGAGCGGGATGTGTTGGTT |
| Primer Seq. 3' |
aatctagaggtaccTCTGCGTAGAAGGGCGTCTCT |
| Predicted Fragment Size |
485 |
| IR fragment full Seq |
0001 GAGGAGCGGT GATGTGTTGG TTTTCGGCGA CCGGCAGTGG ATCACCAACC TTCACTATGC
0061 CTTTCAAGAC AATATTAACC TGTATCTGGT GATGGACTAT TACTGCGGCG GAGACCTGCT
0121 TACATTGCTC AGCAAATTCG AGGACAAGCT GCCCGAGGAC ATGGCCAAGT TCTACATCAC
0181 AGAGATGATC CTGGCAATCA ATAGTATTCA CCAGATTAGG TACGTTCACA GGGACATCAA
0241 GCCGGATAAT GTACTGCTCG ATAAGCGCGG TCACGTGCGC CTGGCTGACT TTGGATCCTG
0301 CCTGCGCCTG GACAAGGATG GCACCGTACA GTCCAACGTG GCCGTGGGCA CACCCGACTA
0361 CATTTCCCCG GAAATTTTGA GGGCCATGGA GGACGGAAAG GGACGCTACG GCACGGAGTG
0421 TGAATTGGTG GTCACTCGGA GTGTGCATGT ACGAGATGCT CTACGGAGAG ACGCCCTTCT
0481 ACGCAGA |
| in silico PCR Fragment |
0001 GAGGAGCGG- GATGTGTTGG TTTTCGGCGA CCGGCAGTGG ATCACCAACC TTCACTATGC
0061 CTTTCAAGAC AATATTAACC TGTATCTGGT GATGGACTAT TACTGCGGCG GAGACCTGCT
0121 TACATTGCTC AGCAAATTCG AGGACAAGCT GCCCGAGGAC ATGGCCAAGT TCTACATCAC
0181 AGAGATGATC CTGGCAATCA ATAGTATTCA CCAGATTAGG TACGTTCACA GGGACATCAA
0241 GCCGGATAAT GTACTGCTCG ATAAGCGCGG TCACGTGCGC CTGGCTGACT TTGGATCCTG
0301 CCTGCGCCTG GACAAGGATG GCACCGTACA GTCCAACGTG GCCGTGGGCA CACCCGACTA
0361 CATTTCCCCG GAAATTTTGA GGGCCATGGA GGACGGAAAG GGACGCTACG GCACGGAGTG
0421 TG-ATTGGTG GTCACTCGGA GTGTGCATGT ACGAGATGCT CTACGGAGAG ACGCCCTTCT
0481 ACGCAGA |
| Assemble Data |
<PRE>
4012R-2.IR_full 1 GAGGAGCGG<span class="snp"><tt>T</tt></span>GATGTGTTGGTTTTCGGCGACCGGCAGTGGATCACCAACCTTCACTATGC 60
|||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
4012R-2.in silico 1 GAGGAGCGG<span class="snp"><tt>-</tt></span>GATGTGTTGGTTTTCGGCGACCGGCAGTGGATCACCAACCTTCACTATGC 60
4012R-2.IR_full 61 CTTTCAAGACAATATTAACCTGTATCTGGTGATGGACTATTACTGCGGCGGAGACCTGCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4012R-2.in silico 61 CTTTCAAGACAATATTAACCTGTATCTGGTGATGGACTATTACTGCGGCGGAGACCTGCT 120
4012R-2.IR_full 121 TACATTGCTCAGCAAATTCGAGGACAAGCTGCCCGAGGACATGGCCAAGTTCTACATCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4012R-2.in silico 121 TACATTGCTCAGCAAATTCGAGGACAAGCTGCCCGAGGACATGGCCAAGTTCTACATCAC 180
4012R-2.IR_full 181 AGAGATGATCCTGGCAATCAATAGTATTCACCAGATTAGGTACGTTCACAGGGACATCAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4012R-2.in silico 181 AGAGATGATCCTGGCAATCAATAGTATTCACCAGATTAGGTACGTTCACAGGGACATCAA 240
4012R-2.IR_full 241 GCCGGATAATGTACTGCTCGATAAGCGCGGTCACGTGCGCCTGGCTGACTTTGGATCCTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4012R-2.in silico 241 GCCGGATAATGTACTGCTCGATAAGCGCGGTCACGTGCGCCTGGCTGACTTTGGATCCTG 300
4012R-2.IR_full 301 CCTGCGCCTGGACAAGGATGGCACCGTACAGTCCAACGTGGCCGTGGGCACACCCGACTA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4012R-2.in silico 301 CCTGCGCCTGGACAAGGATGGCACCGTACAGTCCAACGTGGCCGTGGGCACACCCGACTA 360
4012R-2.IR_full 361 CATTTCCCCGGAAATTTTGAGGGCCATGGAGGACGGAAAGGGACGCTACGGCACGGAGTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4012R-2.in silico 361 CATTTCCCCGGAAATTTTGAGGGCCATGGAGGACGGAAAGGGACGCTACGGCACGGAGTG 420
4012R-2.IR_full 421 TG<span class="snp"><tt>A</tt></span>ATTGGTGGTCACTCGGAGTGTGCATGTACGAGATGCTCTACGGAGAGACGCCCTTCT 480
||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4012R-2.in silico 421 TG<span class="snp"><tt>-</tt></span>ATTGGTGGTCACTCGGAGTGTGCATGTACGAGATGCTCTACGGAGAGACGCCCTTCT 480
4012R-2.IR_full 481 ACGCAGA 487
|||||||
4012R-2.in silico 481 ACGCAGA 487
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
467 |
0 |
0 |
0 |
NM_079113.2 |
CG4012-RA (gek), mRNA |
| 0.42 |
2 |
5 |
3 |
4 |
NM_057401.3 |
CG3068-RA (aur), mRNA |
| 0.42 |
2 |
4 |
6 |
5 |
NM_176397.2 |
CG32944-RB, transcript variant B (CG32944), mRNA |
| 0.42 |
2 |
4 |
6 |
5 |
NM_001043201.1 |
CG32944-RC, transcript variant C (CG32944), mRNA |
| 0.21 |
1 |
3 |
5 |
3 |
NM_170547.2 |
CG31003-RA (gskt), mRNA |
| 0.21 |
1 |
3 |
2 |
3 |
NM_130544.3 |
CG14622-RA, transcript variant A (DAAM), mRNA |
| 0.21 |
1 |
3 |
2 |
3 |
NM_166875.1 |
CG14622-RC, transcript variant C (DAAM), mRNA |
| 0.21 |
1 |
3 |
2 |
3 |
NM_166876.1 |
CG14622-RB, transcript variant B (DAAM), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_080016.2 |
CG4481-RA (Glu-RIB), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_141279.2 |
CG12147-RA (CG12147), mRNA |
| 0 |
0 |
2 |
9 |
2 |
NM_142819.1 |
CG6954-RA (CG6954), mRNA |
| 0 |
0 |
2 |
7 |
7 |
NM_142893.1 |
CG10177-RA (CG10177), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_080046.2 |
CG10986-RB (g), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_167013.1 |
CG3000-RB, transcript variant B (rap), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_080113.2 |
CG3000-RA, transcript variant A (rap), mRNA |
| 0 |
0 |
1 |
7 |
9 |
NM_132566.1 |
CG2577-RA (CG2577), mRNA |
| 0 |
0 |
1 |
5 |
10 |
NM_170524.1 |
CG12072-RA (wts), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_057225.3 |
CG10653-RA (hk), mRNA |
| 0 |
0 |
1 |
4 |
12 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_079863.2 |
CG2048-RC, transcript variant C (dco), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_170535.1 |
CG2048-RA, transcript variant A (dco), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_078585.3 |
CG2028-RB, transcript variant B (CkIalpha), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_167332.1 |
CG2028-RC, transcript variant C (CkIalpha), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_167331.1 |
CG2028-RA, transcript variant A (CkIalpha), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_170536.2 |
CG2048-RB, transcript variant B (dco), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_080076.2 |
CG11770-RA (lin), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_078856.2 |
CG4838-RA (beat-Ic), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079304.2 |
CG11621-RA, transcript variant A (Pi3K68D), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168475.1 |
CG11621-RC, transcript variant C (Pi3K68D), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_139680.1 |
CG17150-RA, transcript variant A (CG17150), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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