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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3874R-1 
 Symbol frc  Full Name fringe connection 
 CG No CG3874  Old CG No CG3874 
 Synonyms UDP-GlcNAc/UDP-GlcUA, ust74C, UST74C, l(3)10098, l(3)02619, l(3)00073, CG3874, l(3)j2E3, l(3)L6750, l(3)S070006, anon-WO0172774.7, anon-WO0172774.5, frc, FRC 
 Accession No (Link to NCBI)  
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees late pharate adult 
 Map Viewer
 Reference
[Please submit your publication]
Yamamoto-Hino M, Yoshida H, Ichimiya T, Sakamura S, Maeda M, Kimura Y, Sasaki N, Aoki-Kinoshita KF, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S, Goto S.
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Genes Cells (2015) 20(6) 521-42 [ PubMed ID = 25940448 ] [ RRC reference ]

Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet. (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgGGCGGAGAGCGATGTCGGAAA 
 Primer Seq. 3'
aatctagaggtaccGAACCGCATTCGAAGGTCGCA 
 Predicted Fragment Size
500 
 IR fragment full Seq
 
 in silico PCR Fragment
 
 Assemble Data

3874R-1.IR full       1   GGCGNAGAGCGATGTCGGAAACAGGGAGCTGGAGGAGAAGATGGGCGGATCGGCGGATCG 60
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-1.in silico     1   GGCGGAGAGCGATGTCGGAAACAGGGAGCTGGAGGAGAAGATGGGCGGATCGGCGGATCG 60


3874R-1.IR full       61  GTCATCGCTGCTCGATGGATCCGGTTCGAAGGAGCTGAGTCACCGGGAACGCGAGGACTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-1.in silico     61  GTCATCGCTGCTCGATGGATCCGGTTCGAAGGAGCTGAGTCACCGGGAACGCGAGGACTC 120


3874R-1.IR full       121 GGCGTTGTTCGTCAAGAAGATCGGGAGCGCCTTGTTCTATGGCTTGTCCTCCTTCATGAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-1.in silico     121 GGCGTTGTTCGTCAAGAAGATCGGGAGCGCCTTGTTCTATGGCTTGTCCTCCTTCATGAT 180


3874R-1.IR full       181 TACGGTGGTAAACAAGACGGTGCTTACCTCCTACCACTTCCCCTCGTTCCTGTTCCTCAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-1.in silico     181 TACGGTGGTAAACAAGACGGTGCTTACCTCCTACCACTTCCCCTCGTTCCTGTTCCTCAG 240


3874R-1.IR full       241 CCTCGGGCAACTTACTGCTAGCATTGTGGTCCTGGGCATGGGCAAGCGCCTGAAATTGGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-1.in silico     241 CCTCGGGCAACTTACTGCTAGCATTGTGGTCCTGGGCATGGGCAAGCGCCTGAAATTGGT 300


3874R-1.IR full       301 GAACTTTCCCCCTCTGCAGAGGAATACCTTCGCCAAGATCTTTCCGCTGCCACTGATATT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-1.in silico     301 GAACTTTCCCCCTCTGCAGAGGAATACCTTCGCCAAGATCTTTCCGCTGCCACTGATATT 360


3874R-1.IR full       361 TCTGGGAAACATGATGTTTNGACTGGGTGGCACAAAAACCTTGAGTCTGCCCATGTTCGC 420
                          ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
3874R-1.in silico     361 TCTGGGAAACATGATGTTTGGACTGGGTGGCACAAAAACCTTGAGTCTGCCCATGTTCGC 420


3874R-1.IR full       421 AGCCCTACGACGCTTCTCTATCCTNATNACCATGCTGCTGGAGCTCAAGATCCTGGGACT 480
                          |||||||||||||||||||||||| || ||||||||||||||||||||||||||||||||
3874R-1.in silico     421 AGCCCTACGACGCTTCTCTATCCTGATGACCATGCTGCTGGAGCTCAAGATCCTGGGACT 480


3874R-1.IR full       481 GCGACCTTCGAATGCGGTTC 500
                          ||||||||||||||||||||
3874R-1.in silico     481 GCGACCTTCGAATGCGGTTC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100  482  NM_079402.2  fringe connection CG3874-RA (frc), mRNA 
0.41  NM_170629.2  CG32352-RC, transcript variant C (CG32352), mRNA 
0.41  NM_168279.2  CG32352-RA, transcript variant A (CG32352), mRNA 
0.41  NM_168278.1  CG32352-RB, transcript variant B (CG32352), mRNA 
NM_132217.2  CG10958-RA (CG10958), mRNA 
NM_132146.1  inx6 CG17063-RA (inx6), mRNA 
NM_057716.3  peanut CG8705-RA, transcript variant A (pnut), mRNA 
NM_165597.1  peanut CG8705-RB, transcript variant B (pnut), mRNA 
NM_143564.1  CG11313-RA (CG11313), mRNA 
NM_167278.1  Kinesin associated protein 3 CG11759-RA (Kap3), mRNA 
NM_166630.1  CG5594-RD, transcript variant D (CG5594), mRNA 
NM_166631.1  CG5594-RA, transcript variant A (CG5594), mRNA 
NM_137605.2  CG13872-RA (CG13872), mRNA 
NM_166313.1  Vha100-3 CG30329-RA (Vha100-3), mRNA 
NM_167063.1  Longevity assurance gene 1 CG3576-RA, transcript variant A (Lag1), mRNA 
NM_144269.2  Longevity assurance gene 1 CG3576-RB, transcript variant B (Lag1), mRNA 
NM_169181.1  CG32468-RA (CG32468), mRNA 
NM_140897.2  Mi-2 CG8103-RA, transcript variant A (Mi-2), mRNA 
NM_001014591.1  Mi-2 CG8103-RB, transcript variant B (Mi-2), mRNA 
NM_169145.1  CG15186-RB, transcript variant B (CG15186), mRNA 
NM_206436.1  CG15186-RC, transcript variant C (CG15186), mRNA 
NM_141402.1  CG15186-RA, transcript variant A (CG15186), mRNA 
NM_080369.2  Glyceraldehyde 3 phosphate dehydrogenase 1 CG12055-RA, transcript variant A (Gapdh1), mRNA 
NM_001038847.1  Glyceraldehyde 3 phosphate dehydrogenase 1 CG12055-RB, transcript variant B (Gapdh1), mRNA 
NM_079067.2  coracle CG11949-RA, transcript variant A (cora), mRNA 
NM_166338.1  coracle CG11949-RD, transcript variant D (cora), mRNA 
NM_166337.1  coracle CG11949-RB, transcript variant B (cora), mRNA 
NM_166336.1  coracle CG11949-RC, transcript variant C (cora), mRNA 
10  NM_169645.2  Tropomyosin 2 CG4843-RC, transcript variant C (Tm2), mRNA 
10  NM_169644.1  Tropomyosin 2 CG4843-RB, transcript variant B (Tm2), mRNA 

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