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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3722R-1 
 Symbol shg  Full Name shotgun 
 CG No CG3722  Old CG No CG3722 
 Synonyms E-cad, E-Cad, Ecad, DCAD2, DE-cadherin, DE-Cad, E-cadherin, DE-cad, Cad, Shg, CT12481, shg/DE-Cad, DE, DE[cyto], DE-CAD, CADH, DCad, DEC, l(2)k10220, l(2)k03401, DECad, D-cad, gp150, l(2)10469, CadE, CG3722, shg, DE-Cadherin, e-cad, DEcad, ECad 
 Accession No (Link to NCBI) NM_057374.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Wang Y, Antunes M, Anderson AE, Kadrmas JL, Jacinto A, Galko MJ.
Integrin Adhesions Suppress Syncytium Formation in the Drosophila Larval Epidermis.
Curr. Biol. (2015) 25(17) 2215-27 [ PubMed ID = 26255846 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Campbell K, Casanova J, Skaer H.
Mesenchymal-to-epithelial transition of intercalating cells in Drosophila renal tubules depends on polarity cues from epithelial neighbours.
Mech. Dev. (2010) 127(7-8) 345-57 [ PubMed ID = 20382220 ] [ RRC reference ]

Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92 [ PubMed ID = 19363474 ] [ RRC reference ]

Maeda K, Takemura M, Umemori M, Adachi-Yamada T.
E-cadherin prolongs the moment for interaction between intestinal stem cell and its progenitor cell to ensure Notch signaling in adult Drosophila midgut.
Genes Cells (2008) 13(12) 1219-27 [ PubMed ID = 19021776 ] [ RRC reference ]

Sasaki N, Sasamura T, Ishikawa HO, Kanai M, Ueda R, Saigo K, Matsuno K.
Polarized exocytosis and transcytosis of Notch during its apical localization in Drosophila epithelial cells.
Genes Cells (2007) 12(1) 89-103 [ PubMed ID = 17212657 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCCAGAAGCTACCACTGCATCA 
 Primer Seq. 3'
aatctagaggtaccCAGTGGGGGCAGACCATTATC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CCAGAAGCTA CCACTGCATC AATATGTCGG CGACCCCGCA GGCCGGCCAC CTAAATCCCG 
0061 CCCAGCAACA GACGCACCAG CAGCACAAGC GAAAATGCCG CGACCTTGGC AGGCGACTAA 
0121 TCCCCGCGAG ACTCCTCCTG GGCGTTATCG TGGCCATCAG CCTTCTGTCA CCCGCCCTCG 
0181 CACTTCACTC GCCGCCGGAC AAAAACTTTT CCGGCGACAA TCGTAAGCCC GCCTTCAAAA 
0241 ACTGCGCCGG ATACGCTCCT AAGGTCAAAG AGGAGCAGCC CGAAAACACC TACGTTCTCA 
0301 CCGTAGAGGC CGTCGACCCT GATCCCGACC AGGTCATTCG ATACAGCATC GTCCAGTCGC 
0361 CGTTCGAGCG ACCCAAGTTC TTTATCAATC CCAGCACGGG CGTAATCTTC ACCACCCACA 
0421 CATTCGATCG TGACGAGCCG ATCCACGAGA AGTTCGTCTT CGTCACCGTC CAGGCCACGG 
0481 ATAATGGTCT GCCCCCACTT G 
 in silico PCR Fragment
0001 CCAGAAGCTA CCACTGCATC AATATGTCGG CGACCCCGCA GGCCGGCCAC CTAAATCCCG 
0061 CCCAGCAACA GACGCACCAG CAGCACAAGC GAAAATGCCG CGACCTTGGC AGGCGACTAA 
0121 TCCCCGCGAG ACTCCTCCTG GGCGTTATCG TGGCCATCAG CCTTCTGTCA CCCGCCCTCG 
0181 CACTTCACTC GCCGCCGGAC AAAAACTTTT CCGGCGACAA TCGTAAGCCC GCCTTCAAAA 
0241 ACTGCGCCGG ATACGCTCCT AAGGTCAAAG AGGAGCAGCC CGAAAACACC TACGTTCTCA 
0301 CCGTAGAGGC CGTCGACCCT GATCCCGACC AGGTCATTCG ATACAGCATC GTCCAGTCGC 
0361 CGTTCGAGCG ACCCAAGTTC TTTATCAATC CCAGCACGGG CGTAATCTTC ACCACCCACA 
0421 CATTCGATCG TGACGAGCCG ATCCACGAGA AGTTCGTCTT CGTCACCGTC CAGGCCACGG 
0481 ATAATGGTCT GCCCCCAC-T G 
 Assemble Data

3722R-1.IR_full       1   CCAGAAGCTACCACTGCATCAATATGTCGGCGACCCCGCAGGCCGGCCACCTAAATCCCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-1.in silico     1   CCAGAAGCTACCACTGCATCAATATGTCGGCGACCCCGCAGGCCGGCCACCTAAATCCCG 60


3722R-1.IR_full       61  CCCAGCAACAGACGCACCAGCAGCACAAGCGAAAATGCCGCGACCTTGGCAGGCGACTAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-1.in silico     61  CCCAGCAACAGACGCACCAGCAGCACAAGCGAAAATGCCGCGACCTTGGCAGGCGACTAA 120


3722R-1.IR_full       121 TCCCCGCGAGACTCCTCCTGGGCGTTATCGTGGCCATCAGCCTTCTGTCACCCGCCCTCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-1.in silico     121 TCCCCGCGAGACTCCTCCTGGGCGTTATCGTGGCCATCAGCCTTCTGTCACCCGCCCTCG 180


3722R-1.IR_full       181 CACTTCACTCGCCGCCGGACAAAAACTTTTCCGGCGACAATCGTAAGCCCGCCTTCAAAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-1.in silico     181 CACTTCACTCGCCGCCGGACAAAAACTTTTCCGGCGACAATCGTAAGCCCGCCTTCAAAA 240


3722R-1.IR_full       241 ACTGCGCCGGATACGCTCCTAAGGTCAAAGAGGAGCAGCCCGAAAACACCTACGTTCTCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-1.in silico     241 ACTGCGCCGGATACGCTCCTAAGGTCAAAGAGGAGCAGCCCGAAAACACCTACGTTCTCA 300


3722R-1.IR_full       301 CCGTAGAGGCCGTCGACCCTGATCCCGACCAGGTCATTCGATACAGCATCGTCCAGTCGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-1.in silico     301 CCGTAGAGGCCGTCGACCCTGATCCCGACCAGGTCATTCGATACAGCATCGTCCAGTCGC 360


3722R-1.IR_full       361 CGTTCGAGCGACCCAAGTTCTTTATCAATCCCAGCACGGGCGTAATCTTCACCACCCACA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-1.in silico     361 CGTTCGAGCGACCCAAGTTCTTTATCAATCCCAGCACGGGCGTAATCTTCACCACCCACA 420


3722R-1.IR_full       421 CATTCGATCGTGACGAGCCGATCCACGAGAAGTTCGTCTTCGTCACCGTCCAGGCCACGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-1.in silico     421 CATTCGATCGTGACGAGCCGATCCACGAGAAGTTCGTCTTCGTCACCGTCCAGGCCACGG 480


3722R-1.IR_full       481 ATAATGGTCTGCCCCCACTTG 501
                          |||||||||||||||||| ||
3722R-1.in silico     481 ATAATGGTCTGCCCCCAC-TG 501

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_057374.2  CG3722-RA (shg), mRNA 
0   19  NM_132412.1  CG9817-RA (CG9817), mRNA 
0   NM_078768.2  CG9554-RB, transcript variant B (eya), mRNA 
0   NM_164693.1  CG9554-RA, transcript variant A (eya), mRNA 
0   NM_167595.3  CG12348-RE, transcript variant E (Sh), mRNA 
0   NM_167594.2  CG12348-RC, transcript variant C (Sh), mRNA 
0   NM_133033.1  CG12672-RA (CG12672), mRNA 
0   NM_139560.2  CG14971-RA (CG14971), mRNA 
0   NM_168088.1  CG32249-RA (CG32249), mRNA 
0   NM_140447.1  CG9598-RA (CG9598), mRNA 
0   NM_206004.1  CG10719-RC, transcript variant C (brat), mRNA 
0   NM_134302.1  CG10719-RB, transcript variant B (brat), mRNA 
0   NM_057597.2  CG10719-RA, transcript variant A (brat), mRNA 
0   NM_167194.1  CG3001-RB, transcript variant B (Hex-A), mRNA 
0   NM_080109.2  CG3001-RA, transcript variant A (Hex-A), mRNA 
0   NM_134690.2  CG2807-RA (CG2807), mRNA 
0   NM_164898.1  CG4916-RB, transcript variant B (me31B), mRNA 
0   NM_001014645.1  CG31156-RB, transcript variant B (CG31156), mRNA 
0   NM_078809.2  CG4916-RA, transcript variant A (me31B), mRNA 
0   NM_140091.2  CG6757-RA (SH3PX1), mRNA 
0   NM_142813.3  CG31156-RA, transcript variant A (CG31156), mRNA 
0   10  NM_132578.1  CG11146-RA (CG11146), mRNA 
0   NM_136941.2  CG8828-RA (CG8828), mRNA 
0   13  18  NM_058149.3  CG3352-RA (ft), mRNA 
0   10  17  NM_206620.4  CG32782-RD, transcript variant D (tlk), mRNA 
0   10  17  NM_130717.3  CG32782-RC, transcript variant C (tlk), mRNA 
0   22  NM_164981.1  CG18789-RA (CG18789), mRNA 
0   16  NM_144407.2  CG18787-RA (CG18787), mRNA 
0   11  NM_132206.1  CG2253-RA (Upf2), mRNA 
0   104  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 

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