National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3705R-1 
 Symbol aay  Full Name astray 
 CG No CG3705  Old CG No CG3705 
 Synonyms 0423/14, CG3705, anon-WO0172774.117, aay 
 Accession No (Link to NCBI) NM_079277.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol. (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgGCAGCCACAAATGGCCATAAT 
 Primer Seq. 3'
aatctagaggtaccGCATAGACGTTTTTCAGGGGA 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 GCAGCCACAA ATGGCCATAA TCTGCTGACC AAGCAGCTGA ATTGCAATGG CAATGGCACC 
0061 aCCGGTGGCG CGGCGAAAAC GACTGTGGCC TCGGCCATAA CACCGCCCAA GCAGCCCCAG 
0121 TTGGCGGCCA AGGTCATCCA GCAGTCGCAG ATCGTCTGTT TCGATGTGGA CTCCACGGTG 
0181 ATTTGCGAGG AGGGCATCGA CGAACTGGCC GAATATTGCG GCAAGGGGAG CGAGGTGGCT 
0241 CGCGTTACCA AGGAGGCGAT GGGCGGTGCC ATGACCTTCC AGGATGCCCT CAAAATCCGC 
0301 TTGAACATCA TACGGCCGAC ACAGCAGCAA GTGAGGGATT TCATCCAGGA GCGACCCAGT 
0361 ACACTGAGCA AAAACGTGAA GCGTTTCGTC AGCCATTTGA AGGCGGAGGG TAAACAGGTT 
0421 TACTTGATCT CCGGCGGATT CGATTGCCTA ATTGCGCCCG TGGCCAATGA ATTGGGTATT 
0481 CCCCTGAAAA ACGTCTATGC  
 in silico PCR Fragment
0001 GCAGCCACAA ATGGCCATAA TCTGCTGACC AAACAGCTGA ATTGCAATGG CAATGGCACC 
0061 ACCGGTGGCG CGGCGAAAAC CACTGTGGCC TCGGCCATAA CACCGCCCAA GCAGCCCCAG 
0121 TTGGCGGCCA AGGTCATCCA GCAGTCGCAG ATCGTCTGTT TCGATGTGGA CTCCACGGTG 
0181 ATTTGCGAGG AGGGCATCGA CGAACTGGCC GAATATTGTG GCAAGGGGAG CGAGGTGGCT 
0241 CGCGTTACCA AGGAGGCGAT GGGCGGTGCC ATGACCTTCC AGGATGCCCT CAAAATTCGC 
0301 TTGAACATCA TACGGCCGAC ACAGCAGCAA GTGAGGGATT TCATCCAGGA GCGACCCAGT 
0361 ACACTGAGCA AAAACGTGAA GCGTTTCGTC AGCCATTTGA AGGCGGAGGG AAAACAGGTT 
0421 TACTTGATCT CCGGCGGATT CGATTGCCTA ATTGCGCCCG TGGCCAATGA ATTGGGTATT 
0481 CCCCTGAAAA ACGTCTATGC  
 Assemble Data

3705R-1.IR_full       1   GCAGCCACAAATGGCCATAATCTGCTGACCAAGCAGCTGAATTGCAATGGCAATGGCACC 60
                          |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
3705R-1.in silico     1   GCAGCCACAAATGGCCATAATCTGCTGACCAAACAGCTGAATTGCAATGGCAATGGCACC 60


3705R-1.IR_full       61  ACCGGTGGCGCGGCGAAAACGACTGTGGCCTCGGCCATAACACCGCCCAAGCAGCCCCAG 120
                          |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
3705R-1.in silico     61  ACCGGTGGCGCGGCGAAAACCACTGTGGCCTCGGCCATAACACCGCCCAAGCAGCCCCAG 120


3705R-1.IR_full       121 TTGGCGGCCAAGGTCATCCAGCAGTCGCAGATCGTCTGTTTCGATGTGGACTCCACGGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3705R-1.in silico     121 TTGGCGGCCAAGGTCATCCAGCAGTCGCAGATCGTCTGTTTCGATGTGGACTCCACGGTG 180


3705R-1.IR_full       181 ATTTGCGAGGAGGGCATCGACGAACTGGCCGAATATTGCGGCAAGGGGAGCGAGGTGGCT 240
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
3705R-1.in silico     181 ATTTGCGAGGAGGGCATCGACGAACTGGCCGAATATTGTGGCAAGGGGAGCGAGGTGGCT 240


3705R-1.IR_full       241 CGCGTTACCAAGGAGGCGATGGGCGGTGCCATGACCTTCCAGGATGCCCTCAAAATCCGC 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
3705R-1.in silico     241 CGCGTTACCAAGGAGGCGATGGGCGGTGCCATGACCTTCCAGGATGCCCTCAAAATTCGC 300


3705R-1.IR_full       301 TTGAACATCATACGGCCGACACAGCAGCAAGTGAGGGATTTCATCCAGGAGCGACCCAGT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3705R-1.in silico     301 TTGAACATCATACGGCCGACACAGCAGCAAGTGAGGGATTTCATCCAGGAGCGACCCAGT 360


3705R-1.IR_full       361 ACACTGAGCAAAAACGTGAAGCGTTTCGTCAGCCATTTGAAGGCGGAGGGTAAACAGGTT 420
                          |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
3705R-1.in silico     361 ACACTGAGCAAAAACGTGAAGCGTTTCGTCAGCCATTTGAAGGCGGAGGGAAAACAGGTT 420


3705R-1.IR_full       421 TACTTGATCTCCGGCGGATTCGATTGCCTAATTGCGCCCGTGGCCAATGAATTGGGTATT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3705R-1.in silico     421 TACTTGATCTCCGGCGGATTCGATTGCCTAATTGCGCCCGTGGCCAATGAATTGGGTATT 480


3705R-1.IR_full       481 CCCCTGAAAAACGTCTATGC 500
                          ||||||||||||||||||||
3705R-1.in silico     481 CCCCTGAAAAACGTCTATGC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_079277.2  CG3705-RA (aay), mRNA 
0   NM_001032403.1  CG7649-RB, transcript variant B (Neu3), mRNA 
0   NM_058150.2  CG2047-RA (ftz), mRNA 
0   NM_001032404.1  CG7649-RA, transcript variant A (Neu3), mRNA 
0   NM_140050.2  CG4022-RA (CG4022), mRNA 
0   NM_001043084.1  CG34123-RD, transcript variant D (CG34123), mRNA 
0   NM_001043083.1  CG34123-RB, transcript variant B (CG34123), mRNA 
0   NM_001043085.1  CG34123-RC, transcript variant C (CG34123), mRNA 
0   NM_144221.2  CG32636-RA (CG32636), mRNA 
0   10  NM_167521.2  CG32575-RA, transcript variant A (hang), mRNA 
0   10  NM_167520.2  CG32575-RB, transcript variant B (hang), mRNA 
0   NM_142755.1  CG5732-RA (CG5732), mRNA 
0   NM_137194.1  CG8179-RA (CG8179), mRNA 
0   NM_139436.1  CG15822-RC (CG15822), mRNA 
0   NM_134932.2  CG2772-RA (CG2772), mRNA 
0   NM_143717.1  CG10840-RB (eIF5B), mRNA 
0   NM_132226.1  CG1583-RA (CG1583), mRNA 
0   NM_058049.4  CG6759-RA (cdc16), mRNA 
0   NM_137713.2  CG4266-RA (CG4266), mRNA 
0   NM_176723.2  CG33175-RG, transcript variant G (spri), mRNA 
0   NM_206678.1  CG33175-RA, transcript variant A (spri), mRNA 
0   NM_176724.1  CG33175-RH, transcript variant H (spri), mRNA 
0   NM_137943.2  CG3493-RA (CG3493), mRNA 
0   NM_165080.1  CG31771-RA (CG31771), mRNA 
0   NM_205953.1  CG4128-RE, transcript variant E (nAcRalpha-30D), mRNA 
0   NM_205952.1  CG4128-RD, transcript variant D (nAcRalpha-30D), mRNA 
0   NM_164874.2  CG4128-RA, transcript variant A (nAcRalpha-30D), mRNA 
0   NM_205951.1  CG4128-RB, transcript variant B (nAcRalpha-30D), mRNA 
0   NM_135472.4  CG4128-RC, transcript variant C (nAcRalpha-30D), mRNA 
0   NM_001038799.1  CG4128-RF, transcript variant F (nAcRalpha-30D), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
   

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.