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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3696R-1 |
| Symbol |
kis |
Full Name |
kismet |
| CG No |
CG3696 |
Old CG No |
CG3696 |
| Synonyms |
kiss, CG3660, KIS, EK2-4, l(2)k16510, GM02209, 5841, 2532, 136/31, l(2)k14112, l(2)k13631, unnamed, l(2)k11324, l(2)s4793, l(2)s4771, l(2)s3527, l(2)k08827, l(2)07812, Su(Pc)21AB, CG18326, CG3696, BEST:GM02209, anon-WO0257455.5, anon-WO0172774.164, kis |
| Accession No (Link to NCBI) |
NM_164376.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Dubruille R, Murad A, Rosbash M, Emery P.
A constant light-genetic screen identifies KISMET as a regulator of circadian photoresponses.
PLoS Genet. (2009) 5(12) e1000787
[
PubMed ID = 20041201
]
[
RRC reference
]
Terriente-Félix A, Molnar C, Gómez-Skarmeta JL, de Celis JF.
A conserved function of the chromatin ATPase Kismet in the regulation of hedgehog expression.
Dev. Biol. (2011) 350(2) 382-92
[
PubMed ID = 21146514
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev. Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTAAACCCATGGACTTCAGCAG |
| Primer Seq. 3' |
aatctagaggtaccGATCCAGTATGAGCTTGGCGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TAAACCCATG GACTTCAGCA GCTTCTCGGC GTTCTGCGAG CATCTTAACA AATCTTCACA
0061 AATTGCAGCA ACAACTGCAA CGACGATACC GCCAACAATA AACAATGGAA AAGGCATTTA
0121 TTTGGAGAAA ATGGAGCGAC AGGGAAAGAT GGAACTGGCG GCGAAGGAGC GCGAGTCCCA
0181 ACGTCTGCAG CTCATTCCCA AAAAATGGAA CCGCCGGGNA AGAATACGAA TTTCTTAGAG
0241 TTCTTACCGG GTATGGTGTG GACCTACACG TCTCAACACC TATGGCTTCC TCTAATGGAA
0301 GTTCCCTTTC GCCCGACTGG ACCAAGTTCA AACAGATGGC TCACCTAGAG AGAAAAAGCG
0361 ATGAAACACT GACGGACTAT TATAAGGTAT TTGTGGCCAT GTGTAAACGA CAGGCGGGTT
0421 TAAAGCTCTC TGAATCGGAG CGCGGCTTAG AAGGTATCAT CGAAGAAATC GAAAAGGAAN
0481 ANGCCAAGCT CATACTGGAT C |
| in silico PCR Fragment |
0001 TAAACCCATG GACTTCAGCA GCTTCTCGGC GTTCTGCGAG CATCTTAACA AATCTTCACA
0061 AATTGCAGCA ACAACTGCAA CGACGATACC GCCAACAATA AACAATGGAA AAGGCATTTA
0121 TTTGGAGAAA ATGGAGCGAC AGGGAAAGAT GGAACTGGCG GCGAAGGAGC GCGAGTCCCA
0181 ACGGCTGCAG CTCATTCCCA AAAAATGGAA CCGCCGGG-A AGAATACGAA TTTCTTAGAG
0241 TTCTTACCGG GTATGGTGTG GACCTACACG TCTCAACACC TATGGCTTCC TCTAATGGAA
0301 GTTCCCTTTC GCCCGACTGG ACCAAGTTCA AACAGATGGC TCACCTAGAG AGAAAAAGCG
0361 ATGAAACACT GACGGACTAT TATAAGGTAT TTGTGGCCAT GTGTAAACGA CAGGCGGGTT
0421 TAAAGCTCTC TGAATCGGAG CGCGGCTTAG AAGGTATCAT CGAAGAAATC GAAAAGGAAC
0481 ACGCCAAGCT CATACTGGAT C |
| Assemble Data |
3696R-1.IR_full 1 TAAACCCATGGACTTCAGCAGCTTCTCGGCGTTCTGCGAGCATCTTAACAAATCTTCACA 60
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3696R-1.in silico 1 TAAACCCATGGACTTCAGCAGCTTCTCGGCGTTCTGCGAGCATCTTAACAAATCTTCACA 60
3696R-1.IR_full 61 AATTGCAGCAACAACTGCAACGACGATACCGCCAACAATAAACAATGGAAAAGGCATTTA 120
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3696R-1.in silico 61 AATTGCAGCAACAACTGCAACGACGATACCGCCAACAATAAACAATGGAAAAGGCATTTA 120
3696R-1.IR_full 121 TTTGGAGAAAATGGAGCGACAGGGAAAGATGGAACTGGCGGCGAAGGAGCGCGAGTCCCA 180
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3696R-1.in silico 121 TTTGGAGAAAATGGAGCGACAGGGAAAGATGGAACTGGCGGCGAAGGAGCGCGAGTCCCA 180
3696R-1.IR_full 181 ACGTCTGCAGCTCATTCCCAAAAAATGGAACCGCCGGGNAAGAATACGAATTTCTTAGAG 240
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3696R-1.in silico 181 ACGGCTGCAGCTCATTCCCAAAAAATGGAACCGCCGGG-AAGAATACGAATTTCTTAGAG 240
3696R-1.IR_full 241 TTCTTACCGGGTATGGTGTGGACCTACACGTCTCAACACCTATGGCTTCCTCTAATGGAA 300
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3696R-1.in silico 241 TTCTTACCGGGTATGGTGTGGACCTACACGTCTCAACACCTATGGCTTCCTCTAATGGAA 300
3696R-1.IR_full 301 GTTCCCTTTCGCCCGACTGGACCAAGTTCAAACAGATGGCTCACCTAGAGAGAAAAAGCG 360
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3696R-1.in silico 301 GTTCCCTTTCGCCCGACTGGACCAAGTTCAAACAGATGGCTCACCTAGAGAGAAAAAGCG 360
3696R-1.IR_full 361 ATGAAACACTGACGGACTATTATAAGGTATTTGTGGCCATGTGTAAACGACAGGCGGGTT 420
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3696R-1.in silico 361 ATGAAACACTGACGGACTATTATAAGGTATTTGTGGCCATGTGTAAACGACAGGCGGGTT 420
3696R-1.IR_full 421 TAAAGCTCTCTGAATCGGAGCGCGGCTTAGAAGGTATCATCGAAGAAATCGAAAAGGAAN 480
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3696R-1.in silico 421 TAAAGCTCTCTGAATCGGAGCGCGGCTTAGAAGGTATCATCGAAGAAATCGAAAAGGAAC 480
3696R-1.IR_full 481 ANGCCAAGCTCATACTGGATC 501
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3696R-1.in silico 481 ACGCCAAGCTCATACTGGATC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_164376.1 |
CG3696-RB, transcript variant B (kis), mRNA |
| 73.65 |
355 |
2 |
1 |
12 |
NM_078717.2 |
CG3696-RA, transcript variant A (kis), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_132573.1 |
CG17788-RA (CG17788), mRNA |
| 0 |
0 |
3 |
5 |
9 |
NM_078797.2 |
CG13109-RA (tai), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_058098.2 |
CG10393-RA (amos), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_137081.2 |
CG30483-RA (Prosap), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_079764.2 |
CG6875-RA (asp), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168059.2 |
CG14995-RA, transcript variant A (CG14995), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168058.2 |
CG14995-RC, transcript variant C (CG14995), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168057.2 |
CG14995-RD, transcript variant D (CG14995), mRNA |
| 0 |
0 |
1 |
8 |
4 |
NM_132072.1 |
CG14446-RA (CG14446), mRNA |
| 0 |
0 |
1 |
7 |
7 |
NM_142180.2 |
CG4285-RA (CG4285), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_135227.1 |
CG11322-RA (CG11322), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_078955.2 |
CG3454-RA (Hdc), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_206321.1 |
CG7590-RB, transcript variant B (scyl), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_001015396.1 |
CG40411-PC.3 (CG40411), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_001015395.1 |
CG40411-PE.3 (CG40411), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168319.1 |
CG4760-RA, transcript variant A (bol), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168320.1 |
CG4760-RC, transcript variant C (bol), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_132538.1 |
CG15737-RA (CG15737), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079369.2 |
CG7599-RA (Eig71Ef), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001015188.1 |
CG40178-PB.3 (CG40178), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001015189.1 |
CG40178-PC.3 (CG40178), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140156.1 |
CG6418-RB (CG6418), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_135783.2 |
CG16820-RA (CG16820), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_169252.1 |
CG11984-RA, transcript variant A (CG11984), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_169253.1 |
CG11984-RC, transcript variant C (CG11984), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_141604.2 |
CG11984-RB, transcript variant B (CG11984), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_132328.1 |
CG17446-RA (CG17446), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_080313.2 |
CG9659-RC, transcript variant C (egh), mRNA |
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