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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3412R-3 |
| Symbol |
slmb |
Full Name |
supernumerary limbs |
| CG No |
CG3412 |
Old CG No |
CG3412 |
| Synonyms |
Slimb, slimb, CG3412, ica, shv, l(3)00295, crd, BcDNA:GM02031, slmb, Slimb/Beta-TRCP1, Slimb/beta-TRCP |
| Accession No (Link to NCBI) |
NM_079706.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Li Y, Sun X, Gao D, Ding Y, Liu J, Chen J, Luo J, Zhang J, Liu Q, Zhou Z.<br> Dual functions of Rack1 in regulating Hedgehog pathway.<br> Cell Death Differ (2020) 27(11) 3082-3096
[
PubMed ID = 32467643
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.<br> In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.<br> PLoS One (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGGCAAGATTGTCTCCGGACT |
| Primer Seq. 3' |
aatctagaggtaccCCACCAGGCGATCTCTGTACT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGGCAAGATT GTCTCCGGAC TGAGGGACAA CACCATCAAG ATCTGGGATC GCACGGATTT
0061 GCAGTGCGTT AAGACCCTAA TGGGCCACAC TGGATCGGTG CTGTNCCTGC AGTATGACGA
0121 CAAGGTGATC ATCAGTGGCT CCAGCGACTC CACCGTCCGC GTGTGGGACG TCAATACCGG
0181 CGAGATGGTC AACACCCTTA TCCACCACTG CGAGGCGGTG CTGCACTTGC GCTTTAACAA
0241 TGGCATGATG GTTACCTGCT CCAAGGATCG CTCCATCGCC GTCTGGGACA TGACCTCACC
0301 CAGCGAGATT ACGCTGCGGC GCGTCCTTGT CGGTCACCGT GCCGCCGTCA ATGTGGTGGA
0361 CTTTGATGAG AAGTACATCG TGTNCGCCAG CGGGGATCGC ACCATCAAGG TCTGGTCCAC
0421 ATCTAGCTGT GAATTCGTGC GCACCTTGAA TgGCCACAAG CGTgGCATCG CCTGCCTACA
0481 GTACAGAGAT CGCCTGGTGG |
| in silico PCR Fragment |
0001 CGGCAAGATT GTCTCCGGAC TGAGGGACAA CACCATCAAG ATCTGGGATC GCACGGATTT
0061 GCAGTGCGTT AAGACCCTAA TGGGCCACAC TGGATCGGTG CTGTGCCTGC AGTATGACGA
0121 CAAGGTGATC ATCAGTGGCT CCAGCGACTC CACCGTCCGC GTGTGGGACG TCAATACCGG
0181 CGAGATGGTC AACACCCTTA TCCACCACTG CGAGGCGGTG CTGCACTTGC GCTTTAACAA
0241 TGGCATGATG GTTACCTGCT CCAAGGATCG CTCCATCGCC GTCTGGGACA TGACCTCACC
0301 CAGCGAGATT ACGCTGCGGC GCGTCCTTGT CGGTCACCGT GCCGCCGTCA ATGTGGTGGA
0361 CTTTGATGAG AAGTACATCG TGTCCGCCAG CGGGGATCGC ACCATCAAGG TCTGGTCCAC
0421 ATCTAGCTGT GAATTCGTGC GCACCTTGAA TGGCCACAAG CGTGGCATCG CCTGCCTACA
0481 GTACAGAGAT CGCCTGGTGG |
| Assemble Data |
<PRE>
3412R-3.IR_full 1 CGGCAAGATTGTCTCCGGACTGAGGGACAACACCATCAAGATCTGGGATCGCACGGATTT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3412R-3.in silico 1 CGGCAAGATTGTCTCCGGACTGAGGGACAACACCATCAAGATCTGGGATCGCACGGATTT 60
3412R-3.IR_full 61 GCAGTGCGTTAAGACCCTAATGGGCCACACTGGATCGGTGCTGT<span class="snp"><tt>N</tt></span>CCTGCAGTATGACGA 120
||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||
3412R-3.in silico 61 GCAGTGCGTTAAGACCCTAATGGGCCACACTGGATCGGTGCTGT<span class="snp"><tt>G</tt></span>CCTGCAGTATGACGA 120
3412R-3.IR_full 121 CAAGGTGATCATCAGTGGCTCCAGCGACTCCACCGTCCGCGTGTGGGACGTCAATACCGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3412R-3.in silico 121 CAAGGTGATCATCAGTGGCTCCAGCGACTCCACCGTCCGCGTGTGGGACGTCAATACCGG 180
3412R-3.IR_full 181 CGAGATGGTCAACACCCTTATCCACCACTGCGAGGCGGTGCTGCACTTGCGCTTTAACAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3412R-3.in silico 181 CGAGATGGTCAACACCCTTATCCACCACTGCGAGGCGGTGCTGCACTTGCGCTTTAACAA 240
3412R-3.IR_full 241 TGGCATGATGGTTACCTGCTCCAAGGATCGCTCCATCGCCGTCTGGGACATGACCTCACC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3412R-3.in silico 241 TGGCATGATGGTTACCTGCTCCAAGGATCGCTCCATCGCCGTCTGGGACATGACCTCACC 300
3412R-3.IR_full 301 CAGCGAGATTACGCTGCGGCGCGTCCTTGTCGGTCACCGTGCCGCCGTCAATGTGGTGGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3412R-3.in silico 301 CAGCGAGATTACGCTGCGGCGCGTCCTTGTCGGTCACCGTGCCGCCGTCAATGTGGTGGA 360
3412R-3.IR_full 361 CTTTGATGAGAAGTACATCGTGT<span class="snp"><tt>N</tt></span>CGCCAGCGGGGATCGCACCATCAAGGTCTGGTCCAC 420
|||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||
3412R-3.in silico 361 CTTTGATGAGAAGTACATCGTGT<span class="snp"><tt>C</tt></span>CGCCAGCGGGGATCGCACCATCAAGGTCTGGTCCAC 420
3412R-3.IR_full 421 ATCTAGCTGTGAATTCGTGCGCACCTTGAATGGCCACAAGCGTGGCATCGCCTGCCTACA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3412R-3.in silico 421 ATCTAGCTGTGAATTCGTGCGCACCTTGAATGGCCACAAGCGTGGCATCGCCTGCCTACA 480
3412R-3.IR_full 481 GTACAGAGATCGCCTGGTGG 500
||||||||||||||||||||
3412R-3.in silico 481 GTACAGAGATCGCCTGGTGG 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_079706.2 |
CG3412-RA (slmb), mRNA |
| 0 |
0 |
5 |
8 |
22 |
NM_057921.3 |
CG7111-RA (Rack1), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_137406.3 |
CG10934-RA (CG10934), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_135556.2 |
CG5168-RA (CG5168), mRNA |
| 0 |
0 |
1 |
3 |
10 |
NM_079078.2 |
CG9707-RA (Acox57D-p), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_135193.2 |
CG9543-RA (epsilonCOP), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_206688.1 |
CG32666-RA, transcript variant A (CG32666), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167288.1 |
CG32666-RB, transcript variant B (CG32666), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136913.2 |
CG17739-RA (CG17739), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079841.2 |
CG17957-RA (Sry-alpha), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137747.1 |
CG15673-RA (CG15673), mRNA |
| 0 |
0 |
0 |
8 |
2 |
NM_079956.2 |
CG18455-RA, transcript variant A (Optix), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_176446.1 |
CG33208-RD, transcript variant D (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_176448.1 |
CG33208-RG, transcript variant G (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_176449.1 |
CG33208-RH, transcript variant H (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_176445.1 |
CG33208-RC, transcript variant C (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_176447.1 |
CG33208-RF, transcript variant F (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_176443.1 |
CG33208-RE, transcript variant E (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_176444.1 |
CG33208-RB, transcript variant B (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_136135.2 |
CG10132-RA (CG10132), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167991.1 |
CG15812-RB, transcript variant B (pfk), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079173.4 |
CG15812-RA, transcript variant A (pfk), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_078676.3 |
CG6223-RA (betaCop), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_078645.2 |
CG4252-RA (mei-41), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_169091.1 |
CG1109-RA, transcript variant A (CG1109), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_169092.1 |
CG1109-RB, transcript variant B (CG1109), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_135978.1 |
CG12288-RA (CG12288), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_168869.1 |
CG4268-RC, transcript variant C (Pitslre), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_140994.2 |
CG4268-RA, transcript variant A (Pitslre), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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