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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3299R-2 |
| Symbol |
Vinc |
Full Name |
Vinculin |
| CG No |
CG3299 |
Old CG No |
CG3299 |
| Synonyms |
vinc, EG:103B4.1, CT11081, CG3299, Dmvincp, Vin2EF, Vin, Vinc |
| Accession No (Link to NCBI) |
NM_057472.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ikawa K, Ishihara S, Tamori Y, Sugimura K.<br> Attachment and detachment of cortical myosin regulates cell junction exchange during cell rearrangement in the Drosophila wing epithelium.<br> Curr Biol (2023) 33(2) 263-275.e4
[
PubMed ID = 36543168
]
[
RRC reference
]
Park SH, Lee CW, Lee JH, Park JY, Roshandell M, Brennan CA, Choe KM.<br> Requirement for and polarized localization of integrin proteins during Drosophila wound closure.<br> Mol Biol Cell (2018) 29(18) 2137-2147
[
PubMed ID = 29995573
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACCTCCTCGCTGCTGCTTTGC |
| Primer Seq. 3' |
aatctagaggtaccGATAGCCACTCGTTGGCCTGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACCTCCTCGC TGCTGCTTTG cTTCGACGAG AGTGAGGTGC GGAAGATCAT CCAGGAGTGC
0061 AAGAGGGTAC TGGACTACTT GGCCGTGGCG GAGGTGATAA ACACCATGGA ACAGCTGGTG
0121 CAGTTCCTCA AGGACCTCTC GCCCTGTCTG AGCAAGGTGC ACCGGGAGGT GGGCGCCCGC
0181 GAGAAGGAGC TGACCCACCA GGTGCACAGC GAGATATTGG TGCGATGCTT GGAGCAAGTG
0241 AAGACCCTGG CCCCAATCCT CATCTGCAGT ATGAAGGTCT ACATCCACAT TGTGGAGCAG
0301 CAGGGCCGCG GCAGCTGAGG AAGCGGCTGA GAACAGGAAC TACCTGGCCG CCAGGATGAG
0361 CGACGAGCTG CAGGAGATCA TCCGCGTGCT GCAGCTGACT ACCCTACGAT GAGGACACCA
0421 GCGAGCTGGA CAACCTCACT GTGCTGAAGA AGCTCTCCAA TGCCATTTCC AACAAAATGG
0481 AGCAGGCCAA CGAGTGGCTA TC |
| in silico PCR Fragment |
0001 ACCTCCTCGC TGCTGCTTTG CTTCGACGAG AGTGAGGTGC GGAAGATCAT CCAGGAGTGC
0061 AAGAGGGTAC TGGACTACTT AGCCGTGGCG GAGGTGATAA ACACCATGGA ACAGCTGGTG
0121 CAGTTCCTCA AGGACCTCTC GCCCTGTCTG AGCAAGGTGC ACCGGGAGGT GGGCGCCCGC
0181 GAGAAGGAGC TGACCCACCA GGTGCACAGC GAGATATTGG TGCGATGCTT GGAGCAAGTG
0241 AAGACCCTGG CCCCAATCCT CATCTGCAGT ATGAAGGTCT ACATCCACAT TGTGGAGCAG
0301 CAGGGCCGCG G-AGCTGAGG AAGCGGCTGA GAACAGGAAC TACCTGGCCG CCAGGATGAG
0361 CGACGAGCTG CAGGAGATCA TCCGCGTGCT GCAGCTGACT A-CCTACGAT GAGGACACCA
0421 GCGAGCTGGA CAACCTCACT GTGCTGAAAA AGCTCTCCAA TGCCATTTCC AACAAAATGG
0481 AGCAGGCCAA CGAGTGGCTA TC |
| Assemble Data |
<PRE>
3299R-2.IR_full 1 ACCTCCTCGCTGCTGCTTTGCTTCGACGAGAGTGAGGTGCGGAAGATCATCCAGGAGTGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3299R-2.in silico 1 ACCTCCTCGCTGCTGCTTTGCTTCGACGAGAGTGAGGTGCGGAAGATCATCCAGGAGTGC 60
3299R-2.IR_full 61 AAGAGGGTACTGGACTACTT<span class="snp"><tt>G</tt></span>GCCGTGGCGGAGGTGATAAACACCATGGAACAGCTGGTG 120
||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||
3299R-2.in silico 61 AAGAGGGTACTGGACTACTT<span class="snp"><tt>A</tt></span>GCCGTGGCGGAGGTGATAAACACCATGGAACAGCTGGTG 120
3299R-2.IR_full 121 CAGTTCCTCAAGGACCTCTCGCCCTGTCTGAGCAAGGTGCACCGGGAGGTGGGCGCCCGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3299R-2.in silico 121 CAGTTCCTCAAGGACCTCTCGCCCTGTCTGAGCAAGGTGCACCGGGAGGTGGGCGCCCGC 180
3299R-2.IR_full 181 GAGAAGGAGCTGACCCACCAGGTGCACAGCGAGATATTGGTGCGATGCTTGGAGCAAGTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3299R-2.in silico 181 GAGAAGGAGCTGACCCACCAGGTGCACAGCGAGATATTGGTGCGATGCTTGGAGCAAGTG 240
3299R-2.IR_full 241 AAGACCCTGGCCCCAATCCTCATCTGCAGTATGAAGGTCTACATCCACATTGTGGAGCAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3299R-2.in silico 241 AAGACCCTGGCCCCAATCCTCATCTGCAGTATGAAGGTCTACATCCACATTGTGGAGCAG 300
3299R-2.IR_full 301 CAGGGCCGCGG<span class="snp"><tt>C</tt></span>AGCTGAGGAAGCGGCTGAGAACAGGAACTACCTGGCCGCCAGGATGAG 360
|||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
3299R-2.in silico 301 CAGGGCCGCGG<span class="snp"><tt>-</tt></span>AGCTGAGGAAGCGGCTGAGAACAGGAACTACCTGGCCGCCAGGATGAG 360
3299R-2.IR_full 361 CGACGAGCTGCAGGAGATCATCCGCGTGCTGCAGCTGACTA<span class="snp"><tt>C</tt></span>CCTACGATGAGGACACCA 420
|||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||
3299R-2.in silico 361 CGACGAGCTGCAGGAGATCATCCGCGTGCTGCAGCTGACTA<span class="snp"><tt>-</tt></span>CCTACGATGAGGACACCA 420
3299R-2.IR_full 421 GCGAGCTGGACAACCTCACTGTGCTGAA<span class="snp"><tt>G</tt></span>AAGCTCTCCAATGCCATTTCCAACAAAATGG 480
||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||
3299R-2.in silico 421 GCGAGCTGGACAACCTCACTGTGCTGAA<span class="snp"><tt>A</tt></span>AAGCTCTCCAATGCCATTTCCAACAAAATGG 480
3299R-2.IR_full 481 AGCAGGCCAACGAGTGGCTATC 502
||||||||||||||||||||||
3299R-2.in silico 481 AGCAGGCCAACGAGTGGCTATC 502
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
6 |
NM_057472.2 |
CG3299-RA (Vinc), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_057473.3 |
CG8987-RA (tam), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_205892.1 |
CG3539-RC, transcript variant C (Slh), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_205891.1 |
CG3539-RD, transcript variant D (Slh), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_167666.1 |
CG14217-RE, transcript variant E (Tao-1), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_167665.1 |
CG14217-RD, transcript variant D (Tao-1), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_141700.1 |
CG16904-RA (CG16904), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_001038815.1 |
CG17988-RB (Ance-3), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_164532.1 |
CG9664-RA, transcript variant A (CG9664), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_164533.1 |
CG9664-RB, transcript variant B (CG9664), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_134915.2 |
CG9664-RC, transcript variant C (CG9664), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_078509.2 |
CG3929-RA (dx), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_176586.2 |
CG7933-RA, transcript variant A (janA), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_176585.1 |
CG7933-RB, transcript variant B (janA), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_206588.1 |
CG7933-RC, transcript variant C (janA), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132116.1 |
CG14441-RA (CG14441), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_139811.1 |
CG10063-RA (CG10063), mRNA |
| 0 |
0 |
1 |
5 |
12 |
NM_169061.1 |
CG10063-RA (CG10063), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA |
| 0 |
0 |
1 |
5 |
10 |
NM_169060.1 |
CG10063-RA (CG10063), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_141795.2 |
CG6693-RA (CG6693), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137832.1 |
CG4554-RA (CG4554), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_170468.1 |
CG31029-RA (CG31029), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_137399.2 |
CG6459-RA (CG6459), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_057951.2 |
CG9210-RA, transcript variant A (Ac13E), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001014745.1 |
CG9210-RB, transcript variant B (Ac13E), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_130663.2 |
CG2675-RA (Csat), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057783.2 |
CG3954-RB, transcript variant B (csw), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
dme04820
:
Cytoskeleton in muscle cells
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