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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

32120R-2

Symbol

sens

Full Name

senseless

CG No

CG32120

Old CG No

CG32120

Synonyms

Sens, Sen, sense, Ly, CG10714, Ly-1, 1228/04, l(3)70Ad, CG32120, sens

Accession No (Link to NCBI)

NM_080079.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Huang YC, Lu YN, Wu JT, Chien CT, Pi H.
The COP9 signalosome converts temporal hormone signaling to spatial restriction on neural competence.
PLoS Genet. (2014) 10(11) e1004760 [ PubMed ID = 25393278 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTACTCGAGTGCCCTACTGTG

Primer Seq. 3'

aatctagaggtaccTCTTCAGGCCGTACTGCTCCC

Predicted Fragment Size

IR fragment full Seq

0001 CTACTCGAGT GCCCTACTGT GGCCCCAATT TTTGTTGTCC TCGGCCACGG CTCTGGGTAC 
0061 ACCACTGACC CCCATGACCC CCAAATCGCC CGCCTCTGTG GTTTTGGGTC AGCGGGATCG 
0121 TGACTTTGCC TTGACGCCCG AAAAGGAGCA TGAACTCCAA ATGAACAATA ACAATGAGAA 
0181 CAGCAAGCAG GACTATCAGG AGCAGGATGA GGATATGCCA CTAAATCTAA GCACCAAGGA 
0241 GCGCATCACA TCGGATGATT CCAACCGAGA TCAGTATCAC AGCAGCAGCA ACAACAGCAG 
0301 CAGGAGCAGT TCCAGCAGTG AAGTGGAGCA ACTGCATCCG ATGACCTCGC TGAACGTTAC 
0361 CCCGCCTCCC CTGAGCGCTG TGAATCTAAA GAGTTCGAGT ACACCACAGC AACAGCGCCA 
0421 GAGATCGCAG GGTAACATCA TCTGGTCACC GGCCTCGATG TGCGAGAGAT CGGCGAGAAG 
0481 GGAGCAGTAC GGCCTGAAGA

in silico PCR Fragment

0001 CTACTCGAGT GCCCTACTGT GGCCCCAATT TTTGTTGTCC TCGGCCACGG CTCTGGGTAC 
0061 ACCACTGACC CCCATGACCC CCAAATCGCC CGCCTCTGTG GTTTTGGGTC AGCGGGATCG 
0121 TGACTTTGCC TTGACGCCCG AAAAGGAGCA TGAACTCCAA ATGAACAATA ACAATGAGAA 
0181 CAGCAAGCAG GACTATCAGG AGCAGGATGA GGATATGCCA CTAAATCTAA GCACCAAGGA 
0241 GCGCATCACA TCGGATGATT CCAACCGAGA TCAGTATCAC AGCAGCAGCA ACAACAGCAG 
0301 CAGGAGCAGT TCCAGCAGTG AAGTGGAGCA ACTGCATCCG ATGACCTCGC TGAACGTTAC 
0361 CCCGCCTCCC CTGAGCGCTG TGAATCTAAA GAGTTCGAGT ACACCACAGC AACAGCGCCA 
0421 GAGATCGCAG GGTAACATCA TCTGGTCACC GGCCTCGATG TGCGAGAGAT CGGCGAGAAG 
0481 GGAGCAGTAC GGCCTGAAGA

Assemble Data


32120R-2.IR_full       1   CTACTCGAGTGCCCTACTGTGGCCCCAATTTTTGTTGTCCTCGGCCACGGCTCTGGGTAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32120R-2.in silico     1   CTACTCGAGTGCCCTACTGTGGCCCCAATTTTTGTTGTCCTCGGCCACGGCTCTGGGTAC 60


32120R-2.IR_full       61  ACCACTGACCCCCATGACCCCCAAATCGCCCGCCTCTGTGGTTTTGGGTCAGCGGGATCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32120R-2.in silico     61  ACCACTGACCCCCATGACCCCCAAATCGCCCGCCTCTGTGGTTTTGGGTCAGCGGGATCG 120


32120R-2.IR_full       121 TGACTTTGCCTTGACGCCCGAAAAGGAGCATGAACTCCAAATGAACAATAACAATGAGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32120R-2.in silico     121 TGACTTTGCCTTGACGCCCGAAAAGGAGCATGAACTCCAAATGAACAATAACAATGAGAA 180


32120R-2.IR_full       181 CAGCAAGCAGGACTATCAGGAGCAGGATGAGGATATGCCACTAAATCTAAGCACCAAGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32120R-2.in silico     181 CAGCAAGCAGGACTATCAGGAGCAGGATGAGGATATGCCACTAAATCTAAGCACCAAGGA 240


32120R-2.IR_full       241 GCGCATCACATCGGATGATTCCAACCGAGATCAGTATCACAGCAGCAGCAACAACAGCAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32120R-2.in silico     241 GCGCATCACATCGGATGATTCCAACCGAGATCAGTATCACAGCAGCAGCAACAACAGCAG 300


32120R-2.IR_full       301 CAGGAGCAGTTCCAGCAGTGAAGTGGAGCAACTGCATCCGATGACCTCGCTGAACGTTAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32120R-2.in silico     301 CAGGAGCAGTTCCAGCAGTGAAGTGGAGCAACTGCATCCGATGACCTCGCTGAACGTTAC 360


32120R-2.IR_full       361 CCCGCCTCCCCTGAGCGCTGTGAATCTAAAGAGTTCGAGTACACCACAGCAACAGCGCCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32120R-2.in silico     361 CCCGCCTCCCCTGAGCGCTGTGAATCTAAAGAGTTCGAGTACACCACAGCAACAGCGCCA 420


32120R-2.IR_full       421 GAGATCGCAGGGTAACATCATCTGGTCACCGGCCTCGATGTGCGAGAGATCGGCGAGAAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32120R-2.in silico     421 GAGATCGCAGGGTAACATCATCTGGTCACCGGCCTCGATGTGCGAGAGATCGGCGAGAAG 480


32120R-2.IR_full       481 GGAGCAGTACGGCCTGAAGA 500
                           ||||||||||||||||||||
32120R-2.in silico     481 GGAGCAGTACGGCCTGAAGA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	4	23	59	NM_080079.2	CG32120-RA (sens), mRNA
7.05	34	130	609	1278	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
7.05	34	130	609	1278	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
7.05	34	130	609	1278	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
4.56	22	37	123	168	NM_166992.2	CG2904-RA (ec), mRNA
4.56	22	36	137	242	NM_169258.1	CG9755-RA, transcript variant A (pum), mRNA
4.56	22	36	137	242	NM_169259.1	CG9755-RD, transcript variant D (pum), mRNA
4.56	22	36	137	242	NM_079561.2	CG9755-RC, transcript variant C (pum), mRNA
4.35	21	60	214	397	NM_135077.2	CG14023-RA (CG14023), mRNA
3.94	19	38	130	242	NM_168179.1	CG32394-RA (CG32394), mRNA
3.31	16	30	103	234	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
3.31	16	30	103	234	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
3.11	15	36	51	116	NM_132328.1	CG17446-RA (CG17446), mRNA
3.11	15	34	121	165	NM_079939.2	CG5580-RA, transcript variant A (sbb), mRNA
2.9	14	54	130	268	NM_137690.2	CG10543-RA, transcript variant A (CG10543), mRNA
2.9	14	54	116	227	NM_176241.1	CG10543-RC, transcript variant C (CG10543), mRNA
2.9	14	54	116	227	NM_166416.1	CG10543-RB, transcript variant B (CG10543), mRNA
2.9	14	39	123	331	NM_143409.1	CG11873-RA (CG11873), mRNA
2.9	14	26	55	183	NM_132114.1	CG14442-RA (CG14442), mRNA
2.9	14	9	36	56	NM_168353.1	CG32048-RB, transcript variant B (CG32048), mRNA
2.9	14	9	36	56	NM_168352.1	CG32048-RA, transcript variant A (CG32048), mRNA
2.69	13	79	165	364	NM_079903.2	CG15319-RB (nej), mRNA
2.69	13	29	51	97	NM_140468.2	CG9425-RA, transcript variant A (CG9425), mRNA
2.69	13	29	51	97	NM_206362.1	CG9425-RB, transcript variant B (CG9425), mRNA
2.69	13	27	66	96	NM_079119.2	CG4354-RA (slbo), mRNA
2.48	12	51	83	143	NM_080487.2	CG10572-RA (Cdk8), mRNA
2.48	12	41	92	133	NM_132126.1	CG3075-RA (CG3075), mRNA
2.48	12	18	36	62	NM_166667.1	CG3411-RA (bs), mRNA
2.28	11	18	120	285	NM_079845.2	CG7951-RA (sima), mRNA
2.28	11	7	30	41	NM_206285.1	CG32393-RB, transcript variant B (dikar), mRNA

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