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Notice:
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Suspension of shipments during Golden Week Holidays: From 4 May to 11 May, deadline 21 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3153R-3 |
| Symbol |
CG3153 |
Full Name |
CG3153 |
| CG No |
CG3153 |
Old CG No |
CG3153 |
| Synonyms |
CT10556, DmML2, CG3153 |
| Accession No (Link to NCBI) |
NM_169568.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Kayukawa N, Miki T.
A genetic screen in Drosophila for regulators of human prostate cancer progression.
Biochem Biophys Res Commun (2014) 451(4) 548-55
[
PubMed ID = 25117438
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTTGTGATCTTCGCCGCTTTG |
| Primer Seq. 3' |
aatctagaggtaccAGCCTTGATTTTGGCGGGTAT |
| Predicted Fragment Size |
456 |
| IR fragment full Seq |
0001 TTTGTGATCT TCGCCGCTTT GATCgGCTTC ACGTCGTCCA CGNATGTGAG TCAGTGCCCG
0061 AAATCCAAAT CGAAGGCCCT AGCTGCCGGT GACGTCTCCA TTTCCAATTG CCCCAAGAGC
0121 AAGTGCATCC TGAAGCGCAA CACGGAGGCC AGCATTCAGA TGAAGATCCG GCCGGAGCGC
0181 GACTTCCAGG AGCTGACCTC CGACATCCAG GGCATTATCC TGGACGTGCC GCTGCCGTTC
0241 CCAGGATATT NACGGCACCA GCGCCTGTCC GCACATCTAC GACGAGGCTG GCGAGAAGAA
0301 GGTGGGCTGC CCACTGAAGG CCGGCCAGGT GTACACCTAC AAGAACAGCT TCAAGATCCT
0361 GCCCGTCTAC CCGACCGTCA GTTTGGAGAT CCACTGGGGA TTGGGCGATA AGCATGGGGA
0421 TGCGGCCTGC TTCCAGATAC CCGCCAAAAT CAAGGCT |
| in silico PCR Fragment |
0001 TTTGTGATCT TCGCCGCTTT GATCGGCTTC ACGTCGTCCA CGGATGTGAG TCAGTGCCCG
0061 AAATCCAAAT CGAAGGCCCT AGCTGCCGGT GACGTCTCCA TTTCCAATTG CCCCAAGAGC
0121 AAGTGCATCC TGAAGCGCAA CACGGAGGCC AGCATTCAGA TGAAGATCCG GCCGGAGCGC
0181 GACTTCCAGG AGCTGACCTC CGACATCCAG GGCATTATCC TGGACGTGCC GCTGCCGTTC
0241 CCAGGATATT -ACGGCACCA GCGCCTGTCC GCACATCTAC GACGAGGCTG GCGAGAAGAA
0301 GGTGGGCTGC CCACTGAAGG CCGGCCAGGT GTACACCTAC AAGAACAGCT TCAAGATCCT
0361 GCCCGTCTAC CCGACCGTCA GTTTGGAGAT CCACTGGGGA TTGGGCGATA AGCATGGGGA
0421 TGCGGCCTGC TTCCAGATAC CCGCCAAAAT CAAGGCT |
| Assemble Data |
3153R-3.IR_full 1 TTTGTGATCTTCGCCGCTTTGATCGGCTTCACGTCGTCCACGNATGTGAGTCAGTGCCCG 60
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
3153R-3.in silico 1 TTTGTGATCTTCGCCGCTTTGATCGGCTTCACGTCGTCCACGGATGTGAGTCAGTGCCCG 60
3153R-3.IR_full 61 AAATCCAAATCGAAGGCCCTAGCTGCCGGTGACGTCTCCATTTCCAATTGCCCCAAGAGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3153R-3.in silico 61 AAATCCAAATCGAAGGCCCTAGCTGCCGGTGACGTCTCCATTTCCAATTGCCCCAAGAGC 120
3153R-3.IR_full 121 AAGTGCATCCTGAAGCGCAACACGGAGGCCAGCATTCAGATGAAGATCCGGCCGGAGCGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3153R-3.in silico 121 AAGTGCATCCTGAAGCGCAACACGGAGGCCAGCATTCAGATGAAGATCCGGCCGGAGCGC 180
3153R-3.IR_full 181 GACTTCCAGGAGCTGACCTCCGACATCCAGGGCATTATCCTGGACGTGCCGCTGCCGTTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3153R-3.in silico 181 GACTTCCAGGAGCTGACCTCCGACATCCAGGGCATTATCCTGGACGTGCCGCTGCCGTTC 240
3153R-3.IR_full 241 CCAGGATATTNACGGCACCAGCGCCTGTCCGCACATCTACGACGAGGCTGGCGAGAAGAA 300
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
3153R-3.in silico 241 CCAGGATATT-ACGGCACCAGCGCCTGTCCGCACATCTACGACGAGGCTGGCGAGAAGAA 300
3153R-3.IR_full 301 GGTGGGCTGCCCACTGAAGGCCGGCCAGGTGTACACCTACAAGAACAGCTTCAAGATCCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3153R-3.in silico 301 GGTGGGCTGCCCACTGAAGGCCGGCCAGGTGTACACCTACAAGAACAGCTTCAAGATCCT 360
3153R-3.IR_full 361 GCCCGTCTACCCGACCGTCAGTTTGGAGATCCACTGGGGATTGGGCGATAAGCATGGGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3153R-3.in silico 361 GCCCGTCTACCCGACCGTCAGTTTGGAGATCCACTGGGGATTGGGCGATAAGCATGGGGA 420
3153R-3.IR_full 421 TGCGGCCTGCTTCCAGATACCCGCCAAAATCAAGGCT 457
|||||||||||||||||||||||||||||||||||||
3153R-3.in silico 421 TGCGGCCTGCTTCCAGATACCCGCCAAAATCAAGGCT 457
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
438 |
0 |
0 |
0 |
NM_142074.2 |
CG3153-RB, transcript variant B (CG3153), mRNA |
| 100 |
438 |
0 |
0 |
0 |
NM_169568.1 |
CG3153-RA, transcript variant A (CG3153), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_137010.1 |
CG4679-RA (CG4679), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_166835.1 |
CG2945-RB, transcript variant B (cin), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_057682.2 |
CG2945-RA, transcript variant A (cin), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_167843.1 |
CG13887-RC, transcript variant C (CG13887), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_138218.2 |
CG13887-RB, transcript variant B (CG13887), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137193.1 |
CG12959-RA (CG12959), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169548.1 |
CG9351-RB, transcript variant B (flfl), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169549.1 |
CG9351-RC, transcript variant C (flfl), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_142047.1 |
CG9351-RA, transcript variant A (flfl), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_134544.1 |
CG11734-RB (HERC2), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_142787.1 |
CG12499-RA (CG12499), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_138130.2 |
CG30420-RB, transcript variant B (Atf-2), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_166690.1 |
CG30420-RA, transcript variant A (Atf-2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_176374.1 |
CG4761-RA (knrl), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206286.1 |
CG10107-RC, transcript variant C (CG10107), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139799.3 |
CG10107-RA, transcript variant A (CG10107), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_132103.2 |
CG3973-RA (CG3973), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_137816.1 |
CG5819-RA, transcript variant A (CG5819), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_166523.1 |
CG5819-RB, transcript variant B (CG5819), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_079643.1 |
CG5178-RA (Act88F), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_166258.1 |
CG5784-RA, transcript variant A (Mapmodulin), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_079056.2 |
CG5784-RB, transcript variant B (Mapmodulin), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_001042869.1 |
CG34125-RA (CG34125), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_206294.1 |
CG33275-RA, transcript variant A (CG33275), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_206295.1 |
CG33275-RB, transcript variant B (CG33275), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_206293.1 |
CG33275-RC, transcript variant C (CG33275), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_143320.2 |
CG18437-RA (CG18437), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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