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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31196R-4 
 Symbol 14-3-3epsilon  Full Name 14-3-3epsilon 
 CG No CG31196  Old CG No CG31196 
 Synonyms PAR5, 14-3-3, par-5, Par-5, D14-3-3epsilon, D14-3-3e, 14-3-3-e, 14-3-3e, EK3-5, CT24092, 14-3-3epsilon, d14-3-3epsilon, SR3-9, l(3)j2B10, Su(Raf)3B, CG31196, anon-WO02059370.52, anon-WO0172774.141 
 Accession No (Link to NCBI) NM_169796.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Karam CS, Kellner WA, Takenaka N, Clemmons AW, Corces VG.
14-3-3 mediates histone cross-talk during transcription elongation in Drosophila.
PLoS Genet. (2010) 6(6) e1000975 [ PubMed ID = 20532201 ] [ RRC reference ]

Le TP, Vuong LT, Kim AR, Hsu YC, Choi KW.
14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila.
Nat Commun (2016) 7 11501 [ PubMed ID = 27151460 ] [ RRC reference ]

Fukui A, Inaki M, Tonoe G, Hamatani H, Homma M, Morimoto T, Aburatani H, Nose A.
Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction.
Biol Open (2012) 1(4) 362-75 [ PubMed ID = 23213426 ] [ RRC reference ]

Tsai CR, Anderson AE, Burra S, Jo J, Galko MJ.
Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.
Dev. Biol. (2017) 427(1) 61-71 [ PubMed ID = 28514643 ] [ RRC reference ]

Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgAATGGTGGAGGCCATGAAGAA 
 Primer Seq. 3'
aatctagaggtaccCGGCGAGTTGAGAATCTCATA 
 Predicted Fragment Size
499 
 IR fragment full Seq
0001 AATGGTGGAG GCCATGAAGA AGGTCGCCTC CATGGACGTA GAGCTGACCG TCGAGGAGCG 
0061 AAATCTGCTG TCGGTGGCGT ACAAGAATGT GATTGGAGCA CGCCCGTCGC CTCGTTGGCG 
0121 CATCATCACC TCGATCGAAC AGAAGGAGGA GAACAAGGGG GCCCGAGGAG AAATTGGAGA 
0181 TGATCAAAAC CTACCGCGGA CAGGTGGAGA AGGAGCTGCG CGACATCTGC TCGGATATAC 
0241 TGAACGTGCT CGAGAAGCAT CTCATTCCAT GCGCCACATC CGGCGAAAGC AAAGTATTCT 
0301 ACTATAAGAT GAAGGGCGAC TACCATCGCT ACCTGGCCGA AtTNNNCACC GGCTCCGAcC 
0361 GCAAGGATGC GGCAGAGAAC TCGCTGATTG CCTACAAGGC GGCCAGCGAT ATTGCCATGA 
0421 ACGATCTGCC ACCAACACAC CCCATCCGTT TGGGCTTGGC ATTGAACTTC TCGGTGTTCT 
0481 ACTATGAGAT TCTCAACTCG CCG 
 in silico PCR Fragment
0001 AATGGTGGAG GCCATGAAGA AGGTCGCCTC CATGGACGTA GAGCTGACCG TCGAGGAGCG 
0061 AAATCTGCTG TCGGTGGCGT ACAAGAATGT GATTGGAGCA CG-CCGT-GC CTCG-TGGCG 
0121 CATCATCACC TCGATCGAAC AGAAGGAGGA GAACAAGGGG G-CCGAGGAG AAATTGGAGA 
0181 TGATCAAAAC CTACCGCGGA CAGGTGGAGA AGGAGCTGCG CGACATCTGC TCGGATATAC 
0241 TGAACGTGCT CGAGAAGCAT CTCATTCCAT GCGCCACATC CGGCGAAAGC AAAGTATTCT 
0301 ACTATAAGAT GAAGGGCGAC TACCATCGCT ACCTGGCCGA ATTCGCCACC GGCTCCGACC 
0361 GCAAGGATGC GGCAGAGAAC TCGCTGATTG CCTACAAGGC GGCCAGCGAT ATTGCCATGA 
0421 ACGATCTGCC ACCAACACAC CCCATCCGTT TGGGCTTGGC ATTGAACTTC TCGGTGTTCT 
0481 ACTATGAGAT TCTCAACTCG CCG 
 Assemble Data

31196R-4.IR_full       1   AATGGTGGAGGCCATGAAGAAGGTCGCCTCCATGGACGTAGAGCTGACCGTCGAGGAGCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-4.in silico     1   AATGGTGGAGGCCATGAAGAAGGTCGCCTCCATGGACGTAGAGCTGACCGTCGAGGAGCG 60


31196R-4.IR_full       61  AAATCTGCTGTCGGTGGCGTACAAGAATGTGATTGGAGCACGCCCGTCGCCTCGTTGGCG 120
                           |||||||||||||||||||||||||||||||||||||||||| |||| |||||| |||||
31196R-4.in silico     61  AAATCTGCTGTCGGTGGCGTACAAGAATGTGATTGGAGCACG-CCGT-GCCTCG-TGGCG 120


31196R-4.IR_full       121 CATCATCACCTCGATCGAACAGAAGGAGGAGAACAAGGGGGCCCGAGGAGAAATTGGAGA 180
                           ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
31196R-4.in silico     121 CATCATCACCTCGATCGAACAGAAGGAGGAGAACAAGGGGG-CCGAGGAGAAATTGGAGA 180


31196R-4.IR_full       181 TGATCAAAACCTACCGCGGACAGGTGGAGAAGGAGCTGCGCGACATCTGCTCGGATATAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-4.in silico     181 TGATCAAAACCTACCGCGGACAGGTGGAGAAGGAGCTGCGCGACATCTGCTCGGATATAC 240


31196R-4.IR_full       241 TGAACGTGCTCGAGAAGCATCTCATTCCATGCGCCACATCCGGCGAAAGCAAAGTATTCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-4.in silico     241 TGAACGTGCTCGAGAAGCATCTCATTCCATGCGCCACATCCGGCGAAAGCAAAGTATTCT 300


31196R-4.IR_full       301 ACTATAAGATGAAGGGCGACTACCATCGCTACCTGGCCGAATTNNNCACCGGCTCCGACC 360
                           |||||||||||||||||||||||||||||||||||||||||||   ||||||||||||||
31196R-4.in silico     301 ACTATAAGATGAAGGGCGACTACCATCGCTACCTGGCCGAATTCGCCACCGGCTCCGACC 360


31196R-4.IR_full       361 GCAAGGATGCGGCAGAGAACTCGCTGATTGCCTACAAGGCGGCCAGCGATATTGCCATGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-4.in silico     361 GCAAGGATGCGGCAGAGAACTCGCTGATTGCCTACAAGGCGGCCAGCGATATTGCCATGA 420


31196R-4.IR_full       421 ACGATCTGCCACCAACACACCCCATCCGTTTGGGCTTGGCATTGAACTTCTCGGTGTTCT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-4.in silico     421 ACGATCTGCCACCAACACACCCCATCCGTTTGGGCTTGGCATTGAACTTCTCGGTGTTCT 480


31196R-4.IR_full       481 ACTATGAGATTCTCAACTCGCCG 503
                           |||||||||||||||||||||||
31196R-4.in silico     481 ACTATGAGATTCTCAACTCGCCG 503

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   481  NM_169796.1  CG31196-RA, transcript variant A (14-3-3epsilon), mRNA 
100   481  NM_169797.1  CG31196-RB, transcript variant B (14-3-3epsilon), mRNA 
100   481  NM_169798.1  CG31196-RD, transcript variant D (14-3-3epsilon), mRNA 
100   481  NM_169799.1  CG31196-RC, transcript variant C (14-3-3epsilon), mRNA 
0   NM_140068.2  CG3428-RA (CG3428), mRNA 
0   22  36  NM_001014516.1  CG17870-RI, transcript variant I (14-3-3zeta), mRNA 
0   22  36  NM_165741.2  CG17870-RA, transcript variant A (14-3-3zeta), mRNA 
0   22  36  NM_001014515.1  CG17870-RJ, transcript variant J (14-3-3zeta), mRNA 
0   22  36  NM_057537.3  CG17870-RD, transcript variant D (14-3-3zeta), mRNA 
0   22  36  NM_165745.2  CG17870-RG, transcript variant G (14-3-3zeta), mRNA 
0   22  36  NM_206070.1  CG17870-RH, transcript variant H (14-3-3zeta), mRNA 
0   22  36  NM_165740.2  CG17870-RE, transcript variant E (14-3-3zeta), mRNA 
0   22  36  NM_165742.1  CG17870-RB, transcript variant B (14-3-3zeta), mRNA 
0   NM_205934.1  CG13391-RB, transcript variant B (Aats-ala), mRNA 
0   NM_078787.2  CG13391-RA, transcript variant A (Aats-ala), mRNA 
0   NM_142668.2  CG3822-RA (CG3822), mRNA 
0   NM_078867.2  CG5526-RA (Dhc36C), mRNA 
0   NM_167364.1  CG15747-RA (CG15747), mRNA 
0   21  NM_166125.2  CG18255-RA, transcript variant A (Strn-Mlck), mRNA 
0   13  NM_166129.2  CG18255-RD, transcript variant D (Strn-Mlck), mRNA 
0   NM_206012.1  CG33322-RA (CG33322), mRNA 
0   NM_140400.1  CG10083-RA (CG10083), mRNA 
0   NM_134486.1  CG14221-RA (CG14221), mRNA 
0   NM_080021.2  CG2952-RA (Dox-A3), mRNA 
0   NM_058091.3  CG3152-RA (Trap1), mRNA 
0   NM_001038808.1  CG17140-RA, transcript variant A (CG17140), mRNA 
0   NM_001038809.1  CG17139-RA, transcript variant A (CG17139), mRNA 
0   14  NM_001043251.1  CG31045-RF, transcript variant F (Mhcl), mRNA 
0   14  NM_169701.2  CG31045-RB, transcript variant B (Mhcl), mRNA 
0   14  NM_169700.2  CG31045-RA, transcript variant A (Mhcl), mRNA 

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