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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2987R-2 |
| Symbol |
alpha-catenin-related |
Full Name |
alpha-catenin related |
| CG No |
CG2987 |
Old CG No |
CG2987 |
| Synonyms |
alpha-catenin-related |
| Accession No (Link to NCBI) |
NM_143816.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
3 lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCTTTCGAGTTCGTGATATGG |
| Primer Seq. 3' |
aatctagaggtaccTCGCTCCTCCTTGAAGGAGTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCTTTCGAGT TCGTGATATG GATAACGAGC AGATCATGTC CACTTTTGGG AACATAGGTC
0061 ACCTGCTGAA CATTGCCGTG GAGCGATTTA TAACCATTGG CGAGATCATC GCCGAGGAGA
0121 ATATTGACAT CAAGGGAGAC ATGTACGAGG CGGCGAAGGA GGCCAGAGAT GCGGGAAAAT
0181 CGATTGAACG ACTTTGTGAT ATTTCACCTC TAAGCGGCAT GGAGCTGCGC CACCACATCG
0241 AGCCCCTTAA GGACTATGGA GCCATCATTT TGGCGGCCCG CTCCCTGCTG TCGTCCGTCA
0301 CGCGCATCCT ACTGCTGGTC GACATCATAG TTGTGAAGAA GCTGCTGACC GCCAAGAAGC
0361 GGGCCTCCGA GAGCCTGGAG AAGCTGGAGT CGGTCATGAA CTTCACGGAA TTCGTGCGCG
0421 CCTTCTCCGT GTTCGGAACC GAGATGATTG AGCTGGCCTA NCTCACGGGC CACCACCGCA
0481 ACTCCTTCAA GGAGGAGCGA |
| in silico PCR Fragment |
0001 GCTTTCGAGT TCGTGATATG GATAACGAGC AGATCATGTC CACTTTTGGG AACATAGGTC
0061 ACCTGCTGAA CATTGCCGTG GAGCGATTTA TAACCATTGG CGAGATCATC GCCGAGGAGA
0121 ATATTGACAT CAAGGGAGAC ATGTACGAGG CGGCGAAGGA GGCCAGAGAT GCGGGAAAAT
0181 CGATTGAACG ACTTTGTGAT ATTTCACCTC TAAGCGGCAT GGAGCTGCGC CACCACATCG
0241 AGCCCCTTAA GGACTATGGA GCCATCATTT TGGCGGCCCG CTCCCTGCTG TCGTCCGTCA
0301 CGCGCATCCT ACTGCTGGTC GACATCATAG TTGTGAAGAA GCTGCTGACC GCCAAGAAGC
0361 GGGCCTCCGA GAGCCTGGAG AAGCTGGAGT CGGTCATGAA CTTCACGGAA TTCGTGCGCG
0421 CCTTCTCCGT GTTCGGAACC GAGATGATTG AGCTGGCCTA CCTCACGGGC CACCACCGCA
0481 ACTCCTTCAA GGAGGAGCGA |
| Assemble Data |
2987R-2.IR_full 1 GCTTTCGAGTTCGTGATATGGATAACGAGCAGATCATGTCCACTTTTGGGAACATAGGTC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2987R-2.in silico 1 GCTTTCGAGTTCGTGATATGGATAACGAGCAGATCATGTCCACTTTTGGGAACATAGGTC 60
2987R-2.IR_full 61 ACCTGCTGAACATTGCCGTGGAGCGATTTATAACCATTGGCGAGATCATCGCCGAGGAGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2987R-2.in silico 61 ACCTGCTGAACATTGCCGTGGAGCGATTTATAACCATTGGCGAGATCATCGCCGAGGAGA 120
2987R-2.IR_full 121 ATATTGACATCAAGGGAGACATGTACGAGGCGGCGAAGGAGGCCAGAGATGCGGGAAAAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2987R-2.in silico 121 ATATTGACATCAAGGGAGACATGTACGAGGCGGCGAAGGAGGCCAGAGATGCGGGAAAAT 180
2987R-2.IR_full 181 CGATTGAACGACTTTGTGATATTTCACCTCTAAGCGGCATGGAGCTGCGCCACCACATCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2987R-2.in silico 181 CGATTGAACGACTTTGTGATATTTCACCTCTAAGCGGCATGGAGCTGCGCCACCACATCG 240
2987R-2.IR_full 241 AGCCCCTTAAGGACTATGGAGCCATCATTTTGGCGGCCCGCTCCCTGCTGTCGTCCGTCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2987R-2.in silico 241 AGCCCCTTAAGGACTATGGAGCCATCATTTTGGCGGCCCGCTCCCTGCTGTCGTCCGTCA 300
2987R-2.IR_full 301 CGCGCATCCTACTGCTGGTCGACATCATAGTTGTGAAGAAGCTGCTGACCGCCAAGAAGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2987R-2.in silico 301 CGCGCATCCTACTGCTGGTCGACATCATAGTTGTGAAGAAGCTGCTGACCGCCAAGAAGC 360
2987R-2.IR_full 361 GGGCCTCCGAGAGCCTGGAGAAGCTGGAGTCGGTCATGAACTTCACGGAATTCGTGCGCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2987R-2.in silico 361 GGGCCTCCGAGAGCCTGGAGAAGCTGGAGTCGGTCATGAACTTCACGGAATTCGTGCGCG 420
2987R-2.IR_full 421 CCTTCTCCGTGTTCGGAACCGAGATGATTGAGCTGGCCTANCTCACGGGCCACCACCGCA 480
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
2987R-2.in silico 421 CCTTCTCCGTGTTCGGAACCGAGATGATTGAGCTGGCCTACCTCACGGGCCACCACCGCA 480
2987R-2.IR_full 481 ACTCCTTCAAGGAGGAGCGA 500
||||||||||||||||||||
2987R-2.in silico 481 ACTCCTTCAAGGAGGAGCGA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_143816.2 |
CG2987-RA, transcript variant A (alpha-catenin-related), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_166641.1 |
CG2987-RB, transcript variant B (alpha-catenin-related), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_132072.1 |
CG14446-RA (CG14446), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_142872.1 |
CG13829-RA (CG13829), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_058149.3 |
CG3352-RA (ft), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_135173.1 |
CG9501-RA (ppk14), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137039.1 |
CG13332-RA (CG13332), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_140663.2 |
CG9705-RA, transcript variant A (CG9705), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_168683.1 |
CG9705-RB, transcript variant B (CG9705), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_137426.2 |
CG5033-RA, transcript variant A (CG5033), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_166248.1 |
CG5033-RB, transcript variant B (CG5033), mRNA |
| 0 |
0 |
1 |
3 |
12 |
NM_169222.2 |
CG31462-RA (CG31462), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137690.2 |
CG10543-RA, transcript variant A (CG10543), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_169419.1 |
CG18476-RA (CG18476), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_135541.1 |
CG13141-RA (CG13141), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141408.3 |
CG1137-RA (CG1137), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_141438.2 |
CG10068-RA (CG10068), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_143721.2 |
CG2128-RA (Hdac3), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_168487.1 |
CG32096-RB, transcript variant B (rols), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_140285.2 |
CG32096-RD, transcript variant D (rols), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_168490.1 |
CG32096-RE, transcript variant E (rols), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_168488.1 |
CG32096-RA, transcript variant A (rols), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_168489.1 |
CG32096-RC, transcript variant C (rols), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169405.2 |
CG31363-RA, transcript variant A (Jupiter), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169404.2 |
CG31363-RD, transcript variant D (Jupiter), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165145.1 |
CG4824-RD, transcript variant D (BicC), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165144.1 |
CG4824-RB, transcript variant B (BicC), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169406.2 |
CG31363-RH, transcript variant H (Jupiter), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057517.2 |
CG4824-RA, transcript variant A (BicC), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080338.3 |
CG6824-RA, transcript variant A (ovo), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
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| DB |
 |
| +- |
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| DC |
 |
| ++ |
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| DM |
 |
| +- |
|
| VM |
 |
| |
|
| VB |
 |
| |
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Caspase-3 signal
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