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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

2916R-1

Symbol

6-Sep

Full Name

6-Sep

CG No

CG2916

Old CG No

CG2916

Synonyms

CG2916, Sep, sep5, Sep5

Accession No (Link to NCBI)

NM_165578.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Sun T, Song Y, Teng D, Chen Y, Dai J, Ma M, Zhang W, Pastor-Pareja JC.<br> Atypical laminin spots and pull-generated microtubule-actin projections mediate Drosophila wing adhesion.<br> Cell Rep (2021) 36(10) 109667 [ PubMed ID = 34496252 ] [ RRC reference ]

Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002 [ PubMed ID = 25977760 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAAGTCCCCAGGCTCCTTCAA

Primer Seq. 3'

aatctagaggtaccTTCACCCTAGTGTCAAGCTGC

Predicted Fragment Size

IR fragment full Seq

0001 AAAGTCCCCA GGCTCCTTCA ACTCGGCGGC CACGTGGGGT TCGACACCCT ACCGGACCAG 
0061 CTGGTCAACA AGAGCGTCCA GAATGGCTTT AGCTTCAATA TCCTATGCAT CGGCGAGACC 
0121 GCTCTCGGGA AATCCACTCT CATGGACACA CTGTTCAATA CCAGTTTTGG ATCTACACCG 
0181 AGCCCGCACA ACCTGCCCAA TGTGAAGCTG AAGGCAAATA CATACGAGCT GCAGGAGAGC 
0241 AACGTCCGCC TGAAGCTGAC CGTCTGTGAC ACGATCGGAT ACGGAGATCA GGTTAATAAA 
0301 GCGGACAGCT ACAAGGCTTT GGTGGAGTAC GTGGACAGCC AGTTTGAAGC GTATTTGCAG 
0361 GAAGAGCTCA AGATCCAGCG AGCGATGGCC AGTGCTCATG ATGGACGCGT TCACGCCTGC 
0421 CTGTACTTCA TCTGTCCCAC GGGCCATGGT CTGAAAGCTA TGGATTTGGT GTGCATGAAG 
0481 CAGCTTGACA CTAGGGTGAA

in silico PCR Fragment

0001 AAAGTCCCCA GGCTCCTTCA ACTCGGCGGC CACGTGGGGT TCGACACCCT ACCGGACCAG 
0061 CTGGTCAACA AGAGCGTCCA GAATGGCTTT AGCTTCAATA TCCTATGCAT CGGCGAGACC 
0121 GCTCTCGGGA AATCCACTCT CATGGACACA CTGTTCAATA CCAGTTTTGG ATCTACACCG 
0181 AGCCCGCACA ACCTGCCCAA TGTGAAGCTG AAGGCAAATA CATACGAGCT GCAGGAGAGC 
0241 AACGTCCGCC TGAAGCTGAC CGTCTGTGAC ACGATCGGAT ACGGAGATCA GGTTAATAAA 
0301 GCGGACAGCT ACAAGGCTTT GGTGGAGTAC GTGGACAGCC AGTTTGAAGC GTATTTGCAG 
0361 GAAGAGCTCA AGATCCAGCG AGCGATGGCC AGTGCTCATG ATGGACGCGT TCACGCCTGC 
0421 CTGTACTTCA TCTGTCCCAC GGGCCATGGT CTGAAAGCTA TGGATTTGGT GTGCATGAAG 
0481 CAGCTTGACA CTAGGGTGAA

Assemble Data

<PRE>

2916R-1.IR_full       1   AAAGTCCCCAGGCTCCTTCAACTCGGCGGCCACGTGGGGTTCGACACCCTACCGGACCAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2916R-1.in silico     1   AAAGTCCCCAGGCTCCTTCAACTCGGCGGCCACGTGGGGTTCGACACCCTACCGGACCAG 60


2916R-1.IR_full       61  CTGGTCAACAAGAGCGTCCAGAATGGCTTTAGCTTCAATATCCTATGCATCGGCGAGACC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2916R-1.in silico     61  CTGGTCAACAAGAGCGTCCAGAATGGCTTTAGCTTCAATATCCTATGCATCGGCGAGACC 120


2916R-1.IR_full       121 GCTCTCGGGAAATCCACTCTCATGGACACACTGTTCAATACCAGTTTTGGATCTACACCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2916R-1.in silico     121 GCTCTCGGGAAATCCACTCTCATGGACACACTGTTCAATACCAGTTTTGGATCTACACCG 180


2916R-1.IR_full       181 AGCCCGCACAACCTGCCCAATGTGAAGCTGAAGGCAAATACATACGAGCTGCAGGAGAGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2916R-1.in silico     181 AGCCCGCACAACCTGCCCAATGTGAAGCTGAAGGCAAATACATACGAGCTGCAGGAGAGC 240


2916R-1.IR_full       241 AACGTCCGCCTGAAGCTGACCGTCTGTGACACGATCGGATACGGAGATCAGGTTAATAAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2916R-1.in silico     241 AACGTCCGCCTGAAGCTGACCGTCTGTGACACGATCGGATACGGAGATCAGGTTAATAAA 300


2916R-1.IR_full       301 GCGGACAGCTACAAGGCTTTGGTGGAGTACGTGGACAGCCAGTTTGAAGCGTATTTGCAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2916R-1.in silico     301 GCGGACAGCTACAAGGCTTTGGTGGAGTACGTGGACAGCCAGTTTGAAGCGTATTTGCAG 360


2916R-1.IR_full       361 GAAGAGCTCAAGATCCAGCGAGCGATGGCCAGTGCTCATGATGGACGCGTTCACGCCTGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2916R-1.in silico     361 GAAGAGCTCAAGATCCAGCGAGCGATGGCCAGTGCTCATGATGGACGCGTTCACGCCTGC 420


2916R-1.IR_full       421 CTGTACTTCATCTGTCCCACGGGCCATGGTCTGAAAGCTATGGATTTGGTGTGCATGAAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2916R-1.in silico     421 CTGTACTTCATCTGTCCCACGGGCCATGGTCTGAAAGCTATGGATTTGGTGTGCATGAAG 480


2916R-1.IR_full       481 CAGCTTGACACTAGGGTGAA 500
                          ||||||||||||||||||||
2916R-1.in silico     481 CAGCTTGACACTAGGGTGAA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_143704.1	CG2916-RB, transcript variant B (Sep5), mRNA
100	482	0	0	0	NM_165578.1	CG2916-RA, transcript variant A (Sep5), mRNA
0.41	2	17	34	54	NM_079693.2	CG4173-RA (Sep2), mRNA
0.2	1	3	6	4	NM_139442.1	CG15878-RA (CG15878), mRNA
0	0	2	2	3	NM_001043260.1	CG34157-RD, transcript variant D (Dys), mRNA
0	0	1	4	5	NM_132749.1	CG9521-RA (CG9521), mRNA
0	0	1	3	5	NM_139913.1	CG13681-RA (CG13681), mRNA
0	0	1	3	5	NM_057999.4	CG4602-RA (Srp54), mRNA
0	0	1	3	4	NM_079192.2	CG15811-RA (Rop), mRNA
0	0	1	3	3	NM_134922.2	CG3285-RA (CG3285), mRNA
0	0	1	3	3	NM_136968.1	CG30052-RA (Obp49a), mRNA
0	0	1	3	2	NM_143437.2	CG2310-RA, transcript variant A (CG2310), mRNA
0	0	1	3	2	NM_170411.1	CG2310-RB, transcript variant B (CG2310), mRNA
0	0	1	2	9	NM_169768.1	CG18212-RA, transcript variant A (CG18212), mRNA
0	0	1	2	9	NM_169769.1	CG18212-RD, transcript variant D (CG18212), mRNA
0	0	1	2	9	NM_142388.2	CG18212-RG, transcript variant G (CG18212), mRNA
0	0	1	2	9	NM_206505.1	CG18212-RB, transcript variant B (CG18212), mRNA
0	0	1	2	9	NM_169771.1	CG18212-RF, transcript variant F (CG18212), mRNA
0	0	1	2	9	NM_169770.2	CG18212-RE, transcript variant E (CG18212), mRNA
0	0	1	2	3	NM_169942.2	CG31189-RA (CG31189), mRNA
0	0	0	5	5	NM_138021.2	CG3860-RA (CG3860), mRNA
0	0	0	4	10	NM_057646.2	CG10844-RD, transcript variant D (Rya-r44F), mRNA
0	0	0	4	10	NM_057645.2	CG10844-RC, transcript variant C (Rya-r44F), mRNA
0	0	0	4	10	NM_057643.2	CG10844-RA, transcript variant A (Rya-r44F), mRNA
0	0	0	4	10	NM_057644.2	CG10844-RB, transcript variant B (Rya-r44F), mRNA
0	0	0	4	9	NM_135564.3	CG6206-RA, transcript variant A (CG6206), mRNA
0	0	0	4	9	NM_164929.1	CG6206-RB, transcript variant B (CG6206), mRNA
0	0	0	4	6	NM_206343.1	CG5620-RB, transcript variant B (CG5620), mRNA
0	0	0	4	6	NM_140304.2	CG5620-RA, transcript variant A (CG5620), mRNA
0	0	0	4	5	NM_132581.1	CG11138-RC, transcript variant C (CG11138), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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