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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2774R-1 
 Symbol CG2774  Full Name CG2774 
 CG No CG2774  Old CG No CG2774 
 Synonyms CG2774 
 Accession No (Link to NCBI)  
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Maruzs T, Lőrincz P, Szatmári Z, Széplaki S, Sándor Z, Lakatos Z, Puska G, Juhász G, Sass M.
Retromer Ensures the Degradation of Autophagic Cargo by Maintaining Lysosome Function in Drosophila.
Traffic (2015) 16(10) 1088-107 [ PubMed ID = 26172538 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgGATCTCTTCGTGTCGGCCTTG 
 Primer Seq. 3'
aatctagaggtaccGTATTCACTGAACGCGGCAGC 
 Predicted Fragment Size
500 
 IR fragment full Seq
 
 in silico PCR Fragment
 
 Assemble Data

2774R-1.IR full       1   GATCTCTTCGTGTCGGCCTTGAGTCCCAGCAGCATCGGGGATATACACCCGCTGCAGGAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2774R-1.in silico     1   GATCTCTTCGTGTCGGCCTTGAGTCCCAGCAGCATCGGGGATATACACCCGCTGCAGGAA 60


2774R-1.IR full       61  GTGCTCACCGACGACGGCGACTACTTCATCAGCATCGTGGTCTCGGATCCGCAGAAAATC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2774R-1.in silico     61  GTGCTCACCGACGACGGCGACTACTTCATCAGCATCGTGGTCTCGGATCCGCAGAAAATC 120


2774R-1.IR full       121 GGCGATGGCATGGGCTCCTATTTGGCCTACAAGGTAACGACCAAAACGAACATTCCCAAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2774R-1.in silico     121 GGCGATGGCATGGGCTCCTATTTGGCCTACAAGGTAACGACCAAAACGAACATTCCCAAA 180


2774R-1.IR full       181 TTCAAGCGTAGCGAGTTCAGTACTCTGCGCCGCTTCAGCGATTTCCTTGGCATCCATGAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2774R-1.in silico     181 TTCAAGCGTAGCGAGTTCAGTACTCTGCGCCGCTTCAGCGATTTCCTTGGCATCCATGAT 240


2774R-1.IR full       241 CTGCTTGTGGGCAAGTACATGCGGCTGGGCCGCATCATACCGCCGGCGCCCTCCAAGAAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2774R-1.in silico     241 CTGCTTGTGGGCAAGTACATGCGGCTGGGCCGCATCATACCGCCGGCGCCCTCCAAGAAC 300


2774R-1.IR full       301 ATAATAGGCAGCACCAAGGTGAAGATTAGCCCGCAGCAAAGCGAACCGGGCACGCCCATG 360
                          || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2774R-1.in silico     301 ATCATAGGCAGCACCAAGGTGAAGATTAGCCCGCAGCAAAGCGAACCGGGCACGCCCATG 360


2774R-1.IR full       361 ACCCAGGAATGGGTGGAGATCCGCCGGGCGGCCCTCGAGCGATTCGTCCACCGTACCGCC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2774R-1.in silico     361 ACCCAGGAATGGGTGGAGATCCGCCGGGCGGCCCTCGAGCGATTCGTCCACCGTACCGCC 420


2774R-1.IR full       421 CAGCATCCGGTGCTGCGCGTCGATCTGGATTTCATGAACTTCCTGGAGAGCGACCAGGAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2774R-1.in silico     421 CAGCATCCGGTGCTGCGCGTCGATCTGGATTTCATGAACTTCCTGGAGAGCGACCAGGAG 480


2774R-1.IR full       481 CTGCCGCGTTCAGTGAATAC 500
                          ||||||||||||||||||||
2774R-1.in silico     481 CTGCCGCGTTCAGTGAATAC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
 

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