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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2411R-1 |
| Symbol |
ptc |
Full Name |
patched |
| CG No |
CG2411 |
Old CG No |
CG2411 |
| Synonyms |
ptc, Ptc, Ptc_Dm, l(2)k02507, rubr, PTC, tuf, pat, Conf, CG2411, BcDNA:RH36596, ptch, Ptch |
| Accession No (Link to NCBI) |
NM_078937.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
larval lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nellas I, Iyer KV, Iglesias-Artola JM, Pippel M, Nadler A, Eaton S, Dye NA.<br> Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc.<br> EMBO Rep (2022) 23(11) e54025
[
PubMed ID = 36134875
]
[
RRC reference
]
Tseng CY, Su YH, Yang SM, Lin KY, Lai CM, Rastegari E, Amartuvshin O, Cho Y, Cai Y, Hsu HJ.<br> Smad-Independent BMP Signaling in Somatic Cells Limits the Size of the Germline Stem Cell Pool.<br> Stem Cell Reports (2018) 11(3) 811-827
[
PubMed ID = 30122445
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Lai CM, Lin KY, Kao SH, Chen YN, Huang F, Hsu HJ.<br> Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion.<br> J Cell Biol (2017) 216(5) 1439-1453
[
PubMed ID = 28363970
]
[
RRC reference
]
Zhang Z, Lv X, Jiang J, Zhang L, Zhao Y.<br> Dual roles of Hh signaling in the regulation of somatic stem cell self-renewal and germline stem cell maintenance in Drosophila testis.<br> Cell Res (2013) 23(4) 573-6
[
PubMed ID = 23419515
]
[
RRC reference
]
Han H, Pan C, Liu C, Lv X, Yang X, Xiong Y, Lu Y, Wu W, Han J, Zhou Z, Jiang H, Zhang L, Zhao Y.<br> Gut-neuron interaction via Hh signaling regulates intestinal progenitor cell differentiation in Drosophila.<br> Cell Discov (2015) 1 15006
[
PubMed ID = 27462407
]
[
RRC reference
]
Lv X, Pan C, Zhang Z, Xia Y, Chen H, Zhang S, Guo T, Han H, Song H, Zhang L, Zhao Y.<br> SUMO regulates somatic cyst stem cell maintenance and directly targets the Hedgehog pathway in adult Drosophila testis.<br> Development (2016) 143(10) 1655-62
[
PubMed ID = 27013244
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Lee G, Liang C, Park G, Jang C, Jung JU, Chung J.<br> UVRAG is required for organ rotation by regulating Notch endocytosis in Drosophila.<br> Dev Biol (2011) 356(2) 588-97
[
PubMed ID = 21729695
]
[
RRC reference
]
Yavari A, Nagaraj R, Owusu-Ansah E, Folick A, Ngo K, Hillman T, Call G, Rohatgi R, Scott MP, Banerjee U.<br> Role of lipid metabolism in smoothened derepression in hedgehog signaling.<br> Dev Cell (2010) 19(1) 54-65
[
PubMed ID = 20643350
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGATAAGGGCAAAGCGCGTGG |
| Primer Seq. 3' |
aatctagaggtaccGCGTGATGATCGAGCACGGAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGATAAGGGC AAAGCGCGTG GCAGCCGCAC GGNGATCTAT CTGCGATCAG TATTCCAGTC
0061 CCACCTCGAA ACCCTCGGCA GGCTCCGTGC AAAAGCACGC GGGGCAAGGT GCTATTCGTG
0121 GCTATCCTGG TGCTGAGCAC CTTCTGCGTC GGCCTGAAGA GCGCCCAGAT CCACTCCAAG
0181 GTGCACCAGC TGTGGATCCA GGAGGGCGGC CGGCTGGAGG CGGAACTGGC CTACACACAG
0241 AAGACGATCG GCGAGGACGA GTCGGCCACG CATCAGCTGC TCATTCAGAC GACCCACGAC
0301 CCGAACGCCT CCGTCCTGCA TCCGCANGCG CTTGCTTGCC CACCTGGAGG TCCTGGTCAA
0361 GGCCACCGCC GTCAAGGTGC ACCTCTACGA CACCGAATGG GGGCTGCGCG ACATGTGCAA
0421 CATGCCGAGC ACGCCNTCCT TCGAGGGCAT CTACTACATC GAGCAGATCC TGCGCCACCT
0481 CATTCCGTGC TCGATCATCN CGN |
| in silico PCR Fragment |
0001 AGATAAGGGC AAAGCGCGTG GCAGCCGCAC GGCGATCTAT CTGCGATCAG TATTCCAGTC
0061 CCACCTCGAA ACCCTCGGCA -GCTCCGTGC AAAAGCACGC -GGGCAAGGT GCTATTCGTG
0121 GCTATCCTGG TGCTGAGCAC CTTCTGCGTC GGCCTGAAGA GCGCCCAGAT CCACTCCAAG
0181 GTGCACCAGC TGTGGATCCA GGAGGGCGGC CGGCTGGAGG CGGAACTGGC CTACACACAG
0241 AAGACGATCG GCGAGGACGA GTCGGCCACG CATCAGCTGC TCATTCAGAC GACCCACGAC
0301 CCGAACGCCT CCGTCCTGCA TCCGCAGGCG C-TGCTTGCC CACCTGGAGG TCCTGGTCAA
0361 GGCCACCGCC GTCAAGGTGC ACCTCTACGA CACCGAATGG GGGCTGCGCG ACATGTGCAA
0421 CATGCCGAGC ACGCCCTCCT TCGAGGGCAT CTACTACATC GAGCAGATCC TGCGCCACCT
0481 CATTCCGTGC TCGATCATCA CGC |
| Assemble Data |
<PRE>
2411R-1.IR_full 1 AGATAAGGGCAAAGCGCGTGGCAGCCGCACGG<span class="snp"><tt>N</tt></span>GATCTATCTGCGATCAGTATTCCAGTC 60
||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||
2411R-1.in silico 1 AGATAAGGGCAAAGCGCGTGGCAGCCGCACGG<span class="snp"><tt>C</tt></span>GATCTATCTGCGATCAGTATTCCAGTC 60
2411R-1.IR_full 61 CCACCTCGAAACCCTCGGCA<span class="snp"><tt>G</tt></span>GCTCCGTGCAAAAGCACGC<span class="snp"><tt>G</tt></span>GGGCAAGGTGCTATTCGTG 120
||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||
2411R-1.in silico 61 CCACCTCGAAACCCTCGGCA<span class="snp"><tt>-</tt></span>GCTCCGTGCAAAAGCACGC<span class="snp"><tt>-</tt></span>GGGCAAGGTGCTATTCGTG 120
2411R-1.IR_full 121 GCTATCCTGGTGCTGAGCACCTTCTGCGTCGGCCTGAAGAGCGCCCAGATCCACTCCAAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2411R-1.in silico 121 GCTATCCTGGTGCTGAGCACCTTCTGCGTCGGCCTGAAGAGCGCCCAGATCCACTCCAAG 180
2411R-1.IR_full 181 GTGCACCAGCTGTGGATCCAGGAGGGCGGCCGGCTGGAGGCGGAACTGGCCTACACACAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2411R-1.in silico 181 GTGCACCAGCTGTGGATCCAGGAGGGCGGCCGGCTGGAGGCGGAACTGGCCTACACACAG 240
2411R-1.IR_full 241 AAGACGATCGGCGAGGACGAGTCGGCCACGCATCAGCTGCTCATTCAGACGACCCACGAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2411R-1.in silico 241 AAGACGATCGGCGAGGACGAGTCGGCCACGCATCAGCTGCTCATTCAGACGACCCACGAC 300
2411R-1.IR_full 301 CCGAACGCCTCCGTCCTGCATCCGCA<span class="snp"><tt>N</tt></span>GCGC<span class="snp"><tt>T</tt></span>TGCTTGCCCACCTGGAGGTCCTGGTCAA 360
||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||
2411R-1.in silico 301 CCGAACGCCTCCGTCCTGCATCCGCA<span class="snp"><tt>G</tt></span>GCGC<span class="snp"><tt>-</tt></span>TGCTTGCCCACCTGGAGGTCCTGGTCAA 360
2411R-1.IR_full 361 GGCCACCGCCGTCAAGGTGCACCTCTACGACACCGAATGGGGGCTGCGCGACATGTGCAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2411R-1.in silico 361 GGCCACCGCCGTCAAGGTGCACCTCTACGACACCGAATGGGGGCTGCGCGACATGTGCAA 420
2411R-1.IR_full 421 CATGCCGAGCACGCC<span class="snp"><tt>N</tt></span>TCCTTCGAGGGCATCTACTACATCGAGCAGATCCTGCGCCACCT 480
|||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||
2411R-1.in silico 421 CATGCCGAGCACGCC<span class="snp"><tt>C</tt></span>TCCTTCGAGGGCATCTACTACATCGAGCAGATCCTGCGCCACCT 480
2411R-1.IR_full 481 CATTCCGTGCTCGATCATC<span class="snp"><tt>N</tt></span>CG<span class="snp"><tt>N</tt></span> 503
|||||||||||||||||||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>
2411R-1.in silico 481 CATTCCGTGCTCGATCATC<span class="snp"><tt>A</tt></span>CG<span class="snp"><tt>C</tt></span> 503
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
4 |
NM_078937.3 |
CG2411-RA (ptc), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_001014668.1 |
CG8318-RD, transcript variant D (Nf1), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_170252.2 |
CG8318-RC, transcript variant C (Nf1), mRNA |
| 0 |
0 |
5 |
2 |
3 |
NM_138115.2 |
CG30421-RA (CG30421), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_001014504.1 |
CG30372-RB (CG30372), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079605.2 |
CG6371-RA (hug), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_134546.3 |
CG11748-RB, transcript variant B (Obp19a), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_167700.1 |
CG11748-RA, transcript variant A (Obp19a), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_132504.1 |
CG11695-RA (CG11695), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141236.2 |
CG2503-RA (atms), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079613.2 |
CG7642-RA (ry), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_144097.2 |
CG15357-RA (CG15357), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_138196.1 |
CG17181-RA (CG17181), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079320.2 |
CG10620-RA (Tsf2), mRNA |
| 0 |
0 |
1 |
2 |
13 |
NM_001042804.1 |
CG2174-RB, transcript variant B (Myo10A), mRNA |
| 0 |
0 |
1 |
2 |
13 |
NM_132441.1 |
CG2174-RA, transcript variant A (Myo10A), mRNA |
| 0 |
0 |
0 |
7 |
9 |
NM_141426.1 |
CG2360-RA (Ccp84Aa), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_206138.1 |
CG15920-RB, transcript variant B (resilin), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_137313.2 |
CG15920-RA, transcript variant A (resilin), mRNA |
| 0 |
0 |
0 |
7 |
6 |
NM_133024.2 |
CG6769-RA (CG6769), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_079840.2 |
CG7938-RA (Sry-beta), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_141425.1 |
CG1252-RA (Ccp84Ab), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_001043201.1 |
CG32944-RC, transcript variant C (CG32944), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_078509.2 |
CG3929-RA (dx), mRNA |
| 0 |
0 |
0 |
5 |
14 |
NM_132390.1 |
CG15306-RA (CG15306), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_143420.2 |
CG11888-RA (Rpn2), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_057922.3 |
CG2699-RA (Pi3K21B), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_139894.1 |
CG8492-RA (CG8492), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_167663.1 |
CG3917-RB, transcript variant B (Grip84), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_078685.2 |
CG3917-RC, transcript variant C (Grip84), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04341
:
Hedgehog signaling pathway - fly
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