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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2262R-1 |
| Symbol |
Smox |
Full Name |
Smad on X |
| CG No |
CG2262 |
Old CG No |
CG2262 |
| Synonyms |
dSmad2, SMAD2, smox, dSMAD2, DSMAD2, smad2, Smad2, Sad, ted, sad, l(1)G0348, tmp, CG2262, Smox, DSmad2 |
| Accession No (Link to NCBI) |
NM_078524.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Zhu S, Chen R, Soba P, Jan YN.
JNK signaling coordinates with ecdysone signaling to promote pruning of Drosophila sensory neuron dendrites.
Development (2019) 146(8)
[
PubMed ID = 30936183
]
[
RRC reference
]
Hevia CF, López-Varea A, Esteban N, de Celis JF.
A Search for Genes Mediating the Growth-Promoting Function of TGFβ in the Drosophila melanogaster Wing Disc.
Genetics (2017) 206(1) 231-249
[
PubMed ID = 28315837
]
[
RRC reference
]
Eusebio N, Tavares L, Pereira PS.
CtBP represses Dpp-dependent Mad activation during Drosophila eye development.
Dev Biol (2018) 442(1) 188-198
[
PubMed ID = 30031756
]
[
RRC reference
]
Hevia CF, de Celis JF.
Activation and function of TGFβ signalling during Drosophila wing development and its interactions with the BMP pathway.
Dev Biol (2013) 377(1) 138-53
[
PubMed ID = 23485686
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGTCAGAGAAGGCCGTCAAG |
| Primer Seq. 3' |
aatctagaggtaccCTGGCAGACGATGATACGGAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGTCAGAGA AGGCCGTCAA GAATCTGGTC AAGAAGATCA AGAAGAATTC GCAGCTGGAG
0061 GAGCTAGAGC GCGCGATCTC CACACAGAAC TGCCAAGACG CGGTGCGTTG ACGGTGCCGC
0121 GCAGCAAGCC AGCTCCCGCC GGCGAGCATC TGCGCAAGGG TCTGCCGCAC GTCATCCTAT
0181 TGCCGCCTGT GGCGCTGGCC CGATCTCCAG AGTCAGAATG AACTAAAGCC CCTCGATCAT
0241 TGCGAGTACG CCTTCCATTT GCGCAAGGAG GAGATCTGCA TAAATCCGTA CCACTACAAA
0301 AAAATTGAAC TGTCCATCCT GGTGCCCAAA TCGCTGCCCA CGCCGCCCGA CTCCATTGTG
0361 GACTATCCGC TGGACAACCA TACGCACCAG ATACCCAACA ACACCGATTA CAATGCGGCG
0421 ATCATTCGCA GCGCCTCGTT GAGTCCGCCG CAGTACATGG AATTGGGCGG TGCCGGGCCC
0481 GTTTCCGTAT CATCGTCTGC CAG |
| in silico PCR Fragment |
0001 TGGTCAGAGA AGGCCGTCAA GAATCTGGTC AAGAAGATCA AGAAGAATTC GCAGCTGGAG
0061 GAGCTAGAGC GCGCGATCTC CACACAGAAC TGCC-AGACG CGGTGCG-TG ACGGTGCCGC
0121 GCAGCAAGCC AGCTCCCGCC GGCGAGCATC TGCGCAAGGG TCTGCCGCAC GTCAT-CTAT
0181 TGCCGCCTGT GGCGCTGGCC CGATCTCCAG AGTCAGAATG AACTAAAGCC CCTCGATCAT
0241 TGCGAGTACG CCTTCCATTT GCGCAAGGAG GAGATCTGCA TAAATCCGTA CCACTACAAA
0301 AAAATTGAAC TGTCCATCCT GGTGCCCAAA TCGCTGCCCA CGCCGCCCGA CTCCATTGTG
0361 GACTATCCGC TGGACAACCA TACGCACCAG ATACCCAACA ACACCGATTA CAATGCGGCG
0421 ATCATTCGCA GCGCCTCGTT GAGTCCGCCG CAGTACATGG AATTGGGCGG TGCCGGGCCC
0481 GTTTCCGTAT CATCGTCTGC CAG |
| Assemble Data |
2262R-1.IR_full 1 TGGTCAGAGAAGGCCGTCAAGAATCTGGTCAAGAAGATCAAGAAGAATTCGCAGCTGGAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2262R-1.in silico 1 TGGTCAGAGAAGGCCGTCAAGAATCTGGTCAAGAAGATCAAGAAGAATTCGCAGCTGGAG 60
2262R-1.IR_full 61 GAGCTAGAGCGCGCGATCTCCACACAGAACTGCCAAGACGCGGTGCGTTGACGGTGCCGC 120
|||||||||||||||||||||||||||||||||| |||||||||||| ||||||||||||
2262R-1.in silico 61 GAGCTAGAGCGCGCGATCTCCACACAGAACTGCC-AGACGCGGTGCG-TGACGGTGCCGC 120
2262R-1.IR_full 121 GCAGCAAGCCAGCTCCCGCCGGCGAGCATCTGCGCAAGGGTCTGCCGCACGTCATCCTAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
2262R-1.in silico 121 GCAGCAAGCCAGCTCCCGCCGGCGAGCATCTGCGCAAGGGTCTGCCGCACGTCAT-CTAT 180
2262R-1.IR_full 181 TGCCGCCTGTGGCGCTGGCCCGATCTCCAGAGTCAGAATGAACTAAAGCCCCTCGATCAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2262R-1.in silico 181 TGCCGCCTGTGGCGCTGGCCCGATCTCCAGAGTCAGAATGAACTAAAGCCCCTCGATCAT 240
2262R-1.IR_full 241 TGCGAGTACGCCTTCCATTTGCGCAAGGAGGAGATCTGCATAAATCCGTACCACTACAAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2262R-1.in silico 241 TGCGAGTACGCCTTCCATTTGCGCAAGGAGGAGATCTGCATAAATCCGTACCACTACAAA 300
2262R-1.IR_full 301 AAAATTGAACTGTCCATCCTGGTGCCCAAATCGCTGCCCACGCCGCCCGACTCCATTGTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2262R-1.in silico 301 AAAATTGAACTGTCCATCCTGGTGCCCAAATCGCTGCCCACGCCGCCCGACTCCATTGTG 360
2262R-1.IR_full 361 GACTATCCGCTGGACAACCATACGCACCAGATACCCAACAACACCGATTACAATGCGGCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2262R-1.in silico 361 GACTATCCGCTGGACAACCATACGCACCAGATACCCAACAACACCGATTACAATGCGGCG 420
2262R-1.IR_full 421 ATCATTCGCAGCGCCTCGTTGAGTCCGCCGCAGTACATGGAATTGGGCGGTGCCGGGCCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2262R-1.in silico 421 ATCATTCGCAGCGCCTCGTTGAGTCCGCCGCAGTACATGGAATTGGGCGGTGCCGGGCCC 480
2262R-1.IR_full 481 GTTTCCGTATCATCGTCTGCCAG 503
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2262R-1.in silico 481 GTTTCCGTATCATCGTCTGCCAG 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_078524.2 |
CG2262-RA (Smox), mRNA |
| 0 |
0 |
6 |
8 |
9 |
NM_079175.2 |
CG1242-RA (Hsp83), mRNA |
| 0 |
0 |
5 |
26 |
46 |
NM_057669.2 |
CG12399-RA (Mad), mRNA |
| 0 |
0 |
4 |
2 |
9 |
NM_132259.2 |
CG11219-RA (PIP82), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_167754.1 |
CG1417-RC, transcript variant C (slgA), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_206803.1 |
CG1417-RH, transcript variant H (slgA), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_078709.2 |
CG1417-RE, transcript variant E (slgA), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_167751.1 |
CG1417-RA, transcript variant A (slgA), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_206804.1 |
CG1417-RG, transcript variant G (slgA), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_206805.1 |
CG1417-RF, transcript variant F (slgA), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_167753.1 |
CG1417-RB, transcript variant B (slgA), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_167752.1 |
CG1417-RD, transcript variant D (slgA), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_140952.1 |
CG5585-RA (CG5585), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_136640.2 |
CG8801-RA (CG8801), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_057412.3 |
CG10223-RA (Top2), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169257.1 |
CG11988-RB, transcript variant B (neur), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_057304.3 |
CG11988-RA, transcript variant A (neur), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167847.1 |
CG18214-RC, transcript variant C (trio), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143703.2 |
CG18214-RA, transcript variant A (trio), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_144382.2 |
CG3021-RA (CG3021), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167007.1 |
CG2982-RA, transcript variant A (CG2982), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_131932.2 |
CG2982-RB, transcript variant B (CG2982), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_132968.1 |
CG5010-RA (CG5010), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_137155.2 |
CG10228-RA (Pcf11), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165685.1 |
CG30338-RA (CG30338), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_078671.3 |
CG7098-RA (dik), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_142834.2 |
CG6985-RA (CG6985), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_078908.2 |
CG12845-RA (Tsp42Ef), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_057378.2 |
CG4200-RA (sl), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_141694.1 |
CG8516-RA (CG8516), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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JNK signal
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Caspase-3 signal
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