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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

2010R-2

Symbol

CG2010

Full Name

CG2010

CG No

CG2010

Old CG No

CG2010

Synonyms

CG2010

Accession No (Link to NCBI)

NM_170412.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Chen ZS, Wong AKY, Cheng TC, Koon AC, Chan HYE.<br> FipoQ/FBXO33, a Cullin-1-based ubiquitin ligase complex component modulates ubiquitination and solubility of polyglutamine disease protein.<br> J Neurochem (2019) 149(6) 781-798 [ PubMed ID = 30685895 ] [ RRC reference ]

Dui W, Lu W, Ma J, Jiao R.<br> A systematic phenotypic screen of F-box genes through a tissue-specific RNAi-based approach in Drosophila.<br> J Genet Genomics (2012) 39(8) 397-413 [ PubMed ID = 22884096 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTACATCGAGCTGCCCATCGAG

Primer Seq. 3'

aatctagaggtaccAGGCAGGTGAGGCGCTTGGAA

Predicted Fragment Size

IR fragment full Seq

0001 TACATCGAGC TGCCCATCGA GCTGATCACG CATACGGTGC TCCACGAACT GTCCGCCAAG 
0061 TGCCCCAATC TGACCCACAT GCTGCTGGAC TTCTCCACAG CCATGCAGCT GCACGACTTC 
0121 AGCGAGATGC AGGCCTTTCC CACCAAGCTG CGCTACATGT GCGTCTGCCT CTCGGAGGTG 
0181 ATCTTCATGG AGGGTTTCAT GCGCAAAATC TACAACTTCA TCAACGGCCT GGAGGTGCTG 
0241 CATCTAATTG GCACGTACGA GAAGTGCGAG GAGGAGGAGG AGGAGATCTA CGAGGTGATC 
0301 AACGTGCACA AGCTCAANGT CGGCCACGCC CAATTTGCGT GTGATCAACC TGTACGGCAT 
0361 CAACTTCATC GATGACTCGC ACATCGNACG CCTTCAGCTC CAATTGCATC CAGCTGGAGT 
0421 GCCTGGCCGT CAACTTCTGC AACAAAGTGA CCGGCTCCAC CCTGAAGACC CTGATCCAGC 
0481 GTTCCAAGCG CCTCACCTGC CT

in silico PCR Fragment

0001 TACATCGAGC TGCCCATCGA GCTGATCACG CACACGGTGC TCCACGAACT GTCCGCCAAG 
0061 TGCCCCAATT TGACCCACAT GCTGCTGGAC TTCTCCACAG CCATGCAGCT GCACGACTTC 
0121 AGCGAGATGC AGGCCTTTCC CACCAAGCTG CGCTACATGT GCGTCTGCCT CTCGGAGGTG 
0181 ATCTTCATGG AGGGCTTCAT GCGCAAAATC TACAACTTCA TCAACGGCCT GGAGGTGCTG 
0241 CATCTAATTG GCACGTACGA GAAGTGCGAG GAGGAGGAGG AGGAGATCTA CGAGGTGATC 
0301 AACGTGCACA AGCTCAA-GT CGGCCACGCC CAATTTGCGT GTGATCAACC TGTACGGCAT 
0361 CAACTTCATC GATGACTCGC ACATCG-ACG CCTTCAGCTC GAACTGCATC CAGCTGGAGT 
0421 GCCTGGCCGT CAACTTCTGC AACAAAGTGA CCGGCTCCAC CCTGAAGACC CTGATCCAGC 
0481 GTTCCAAGCG CCTCACCTGC CT

Assemble Data

<PRE>

2010R-2.IR_full       1   TACATCGAGCTGCCCATCGAGCTGATCACGCA<span class="snp"><tt>T</tt></span>ACGGTGCTCCACGAACTGTCCGCCAAG 60
                          ||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||
2010R-2.in silico     1   TACATCGAGCTGCCCATCGAGCTGATCACGCA<span class="snp"><tt>C</tt></span>ACGGTGCTCCACGAACTGTCCGCCAAG 60


2010R-2.IR_full       61  TGCCCCAAT<span class="snp"><tt>C</tt></span>TGACCCACATGCTGCTGGACTTCTCCACAGCCATGCAGCTGCACGACTTC 120
                          |||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
2010R-2.in silico     61  TGCCCCAAT<span class="snp"><tt>T</tt></span>TGACCCACATGCTGCTGGACTTCTCCACAGCCATGCAGCTGCACGACTTC 120


2010R-2.IR_full       121 AGCGAGATGCAGGCCTTTCCCACCAAGCTGCGCTACATGTGCGTCTGCCTCTCGGAGGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010R-2.in silico     121 AGCGAGATGCAGGCCTTTCCCACCAAGCTGCGCTACATGTGCGTCTGCCTCTCGGAGGTG 180


2010R-2.IR_full       181 ATCTTCATGGAGGG<span class="snp"><tt>T</tt></span>TTCATGCGCAAAATCTACAACTTCATCAACGGCCTGGAGGTGCTG 240
                          ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||
2010R-2.in silico     181 ATCTTCATGGAGGG<span class="snp"><tt>C</tt></span>TTCATGCGCAAAATCTACAACTTCATCAACGGCCTGGAGGTGCTG 240


2010R-2.IR_full       241 CATCTAATTGGCACGTACGAGAAGTGCGAGGAGGAGGAGGAGGAGATCTACGAGGTGATC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010R-2.in silico     241 CATCTAATTGGCACGTACGAGAAGTGCGAGGAGGAGGAGGAGGAGATCTACGAGGTGATC 300


2010R-2.IR_full       301 AACGTGCACAAGCTCAA<span class="snp"><tt>N</tt></span>GTCGGCCACGCCCAATTTGCGTGTGATCAACCTGTACGGCAT 360
                          |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
2010R-2.in silico     301 AACGTGCACAAGCTCAA<span class="snp"><tt>-</tt></span>GTCGGCCACGCCCAATTTGCGTGTGATCAACCTGTACGGCAT 360


2010R-2.IR_full       361 CAACTTCATCGATGACTCGCACATCG<span class="snp"><tt>N</tt></span>ACGCCTTCAGCTC<span class="snp"><tt>C</tt></span>AA<span class="snp"><tt>T</tt></span>TGCATCCAGCTGGAGT 420
                          ||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
2010R-2.in silico     361 CAACTTCATCGATGACTCGCACATCG<span class="snp"><tt>-</tt></span>ACGCCTTCAGCTC<span class="snp"><tt>G</tt></span>AA<span class="snp"><tt>C</tt></span>TGCATCCAGCTGGAGT 420


2010R-2.IR_full       421 GCCTGGCCGTCAACTTCTGCAACAAAGTGACCGGCTCCACCCTGAAGACCCTGATCCAGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010R-2.in silico     421 GCCTGGCCGTCAACTTCTGCAACAAAGTGACCGGCTCCACCCTGAAGACCCTGATCCAGC 480


2010R-2.IR_full       481 GTTCCAAGCGCCTCACCTGCCT 502
                          ||||||||||||||||||||||
2010R-2.in silico     481 GTTCCAAGCGCCTCACCTGCCT 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_143439.2	CG2010-RB, transcript variant B (CG2010), mRNA
100	482	0	0	3	NM_170412.1	CG2010-RA, transcript variant A (CG2010), mRNA
0.62	3	4	15	16	NM_167445.2	CG6294-RA (CG6294), mRNA
0.41	2	5	6	9	NM_140421.2	CG17361-RA (CG17361), mRNA
0.41	2	4	7	11	NM_141595.2	CG11971-RA (CG11971), mRNA
0.41	2	2	7	16	NM_001031964.1	CG13702-RC, transcript variant C (AlCR2), mRNA
0.41	2	2	7	16	NM_140782.4	CG13702-RB, transcript variant B (AlCR2), mRNA
0.2	1	10	11	23	NM_167309.1	CG32663-RA (CG32663), mRNA
0.2	1	8	19	38	NM_132903.1	CG9906-RA (CG9906), mRNA
0.2	1	8	17	44	NM_133114.2	CG32541-RA (CG32541), mRNA
0.2	1	8	11	26	NM_164640.1	CG14021-RA, transcript variant A (CG14021), mRNA
0.2	1	8	11	26	NM_135079.4	CG14021-RB, transcript variant B (CG14021), mRNA
0.2	1	7	10	19	NM_143222.1	CG14237-RA (CG14237), mRNA
0.2	1	6	12	25	NM_057368.3	CG2621-RD, transcript variant D (sgg), mRNA
0.2	1	6	11	22	NM_140288.2	CG6801-RA (l(3)j2D3), mRNA
0.2	1	6	10	17	NM_134510.1	CG12703-RA (CG12703), mRNA
0.2	1	6	6	15	NM_143000.1	CG17782-RA (CG17782), mRNA
0.2	1	5	14	21	NM_136942.2	CG8545-RA (CG8545), mRNA
0.2	1	5	10	27	NM_132529.2	CG2025-RA (CG2025), mRNA
0.2	1	5	4	7	NM_001015181.1	CG12459-PA (CG12459), mRNA
0.2	1	4	26	36	NM_141129.1	CG11523-RA (CG11523), mRNA
0.2	1	4	11	51	NM_138025.2	CG3121-RA (CG3121), mRNA
0.2	1	4	8	21	NM_206356.2	CG33261-RB, transcript variant B (Trl), mRNA
0.2	1	4	8	21	NM_001038926.1	CG33261-RI, transcript variant I (Trl), mRNA
0.2	1	4	7	17	NM_079295.2	CG6539-RA (Dhh1), mRNA
0.2	1	4	7	11	NM_132645.2	CG15745-RA, transcript variant A (CG15745), mRNA
0.2	1	4	7	11	NM_167357.1	CG15745-RB, transcript variant B (CG15745), mRNA
0.2	1	4	6	20	NM_001014617.1	CG7583-RE, transcript variant E (CtBP), mRNA
0.2	1	4	6	15	NM_170228.1	CG4548-RA, transcript variant A (XNP), mRNA
0.2	1	4	6	15	NM_143141.2	CG4548-RB, transcript variant B (XNP), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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