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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1950R-1 |
| Symbol |
CG1950 |
Full Name |
CG1950 |
| CG No |
CG1950 |
Old CG No |
CG1950 |
| Synonyms |
CG1950 |
| Accession No (Link to NCBI) |
NM_132553.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Fernández-Espartero CH, Rizzo A, Fulford AD, Falo-Sanjuan J, Goutte-Gattat D, Ribeiro PS.<br> Prp8 regulates oncogene-induced hyperplastic growth in <i>Drosophila</i>.<br> Development (2018) 145(22)
[
PubMed ID = 30333215
]
[
RRC reference
]
Zhang J, Liu M, Su Y, Du J, Zhu AJ.<br> A targeted in vivo RNAi screen reveals deubiquitinases as new regulators of Notch signaling.<br> G3 (Bethesda) (2012) 2(12) 1563-75
[
PubMed ID = 23275879
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGACCCGAACTCTTCGTGGTTG |
| Primer Seq. 3' |
aatctagaggtaccGACCGATCCTTGGCCTTGTCA |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 NACCCGAACT CTTCGTGGTT GAAGAGAGCA CGGATTTCAT AGAAGATGAT TGTTACCACT
0061 TTGTGGGCTT TATGCCAATA AAAGGCAAAC TTTTCGAATT GGACGGAATG CATGAGGGTC
0121 CCATCGAACT GGCCGATATC GACCAGCAAC AGAACTGGCT GGATGTGGTC AGACCGATTA
0181 TTGAGGCACG CATGGAANGC TACAGCGTCG GTGAGATCCA CTTCAATCTA ATGGCCCTGG
0241 TCTCGGATCG TCAGCGATGC TACGAGCGGA AGATCCAAAT GCTGGTCAAC CTACCATCGC
0301 AACNAAGCCA CNCGGATCGT CAGGCGGAGA TCGCCAACCT AAGATCCCAT GTGAGGCACG
0361 AGAAGGAGAA GAAGCGTcGC TNTCGCAAGG AGAACATTCG TCGCCGCCAC AATTATCTGC
0421 CCTTCATTGT TGAGCTACTA AAGCAGCTGG GCGAGACTGG CCAATTGATG GCCATTTGTG
0481 ACAAGGCCAA GGATCGGTC |
| in silico PCR Fragment |
0001 GACCCGAACT CTTCGTGGTT GAAGAGAGCA CGGATTTCAT AGAAGATGAT TGTTACCACT
0061 TTGTGGGCTT TATGCCAATA AAAGGCAAAC TTTTCGAATT GGACGGAATG CATGAGGGTC
0121 CCATCGAACT GGCCGATATC GACCAGCAAC AGAACTGGCT GGATGTGGTC AGACCGATTA
0181 TTGAGGCACG CATGGAACGC TACAGCGTCG GTGAGATCCA CTTCAATCTA ATGGCCCTGG
0241 TCTCGGATCG TCAGCGATGC TACGAGCGGA AGATCCAAAT GCTGGTCAAC CTACCATCGC
0301 AACTAAGCCA CGCGGATCGT CAGGCGGAGA TCGCCAACCT AAGATCCCAT GTGAGGCACG
0361 AGAAGGAGAA GAAGCGTCGC TATCGCAAGG AGAACATTCG TCGCCGCCAC AATTATCTGC
0421 CCTTCATTGT TGAGCTACTA AAGCAGCTGG GCGAGACTGG CCAATTGATG GCCATTTGTG
0481 ACAAGGCCAA GGATCGGTC |
| Assemble Data |
<PRE>
1950R-1.IR_full 1 ACCCGAACTCTTCGTGGTTGAAGAGAGCACGGATTTCATAGAAGATGATTGTTACCACT 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1950R-1.in silico 1 ACCCGAACTCTTCGTGGTTGAAGAGAGCACGGATTTCATAGAAGATGATTGTTACCACT 59
1950R-1.IR_full 61 TTGTGGGCTTTATGCCAATAAAAGGCAAACTTTTCGAATTGGACGGAATGCATGAGGGTC 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1950R-1.in silico 61 TTGTGGGCTTTATGCCAATAAAAGGCAAACTTTTCGAATTGGACGGAATGCATGAGGGTC 119
1950R-1.IR_full 121 CCATCGAACTGGCCGATATCGACCAGCAACAGAACTGGCTGGATGTGGTCAGACCGATTA 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1950R-1.in silico 121 CCATCGAACTGGCCGATATCGACCAGCAACAGAACTGGCTGGATGTGGTCAGACCGATTA 179
1950R-1.IR_full 181 TTGAGGCACGCATGGAA<span class="snp"><tt>N</tt></span>GCTACAGCGTCGGTGAGATCCACTTCAATCTAATGGCCCTGG 239
|||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||
1950R-1.in silico 181 TTGAGGCACGCATGGAA<span class="snp"><tt>C</tt></span>GCTACAGCGTCGGTGAGATCCACTTCAATCTAATGGCCCTGG 239
1950R-1.IR_full 241 TCTCGGATCGTCAGCGATGCTACGAGCGGAAGATCCAAATGCTGGTCAACCTACCATCGC 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1950R-1.in silico 241 TCTCGGATCGTCAGCGATGCTACGAGCGGAAGATCCAAATGCTGGTCAACCTACCATCGC 299
1950R-1.IR_full 301 AAC<span class="snp"><tt>N</tt></span>AAGCCAC<span class="snp"><tt>N</tt></span>CGGATCGTCAGGCGGAGATCGCCAACCTAAGATCCCATGTGAGGCACG 359
|||<span class="snp"><tt> </tt></span>|||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
1950R-1.in silico 301 AAC<span class="snp"><tt>T</tt></span>AAGCCAC<span class="snp"><tt>G</tt></span>CGGATCGTCAGGCGGAGATCGCCAACCTAAGATCCCATGTGAGGCACG 359
1950R-1.IR_full 361 AGAAGGAGAAGAAGCGTCGCT<span class="snp"><tt>N</tt></span>TCGCAAGGAGAACATTCGTCGCCGCCACAATTATCTGC 419
|||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||
1950R-1.in silico 361 AGAAGGAGAAGAAGCGTCGCT<span class="snp"><tt>A</tt></span>TCGCAAGGAGAACATTCGTCGCCGCCACAATTATCTGC 419
1950R-1.IR_full 421 CCTTCATTGTTGAGCTACTAAAGCAGCTGGGCGAGACTGGCCAATTGATGGCCATTTGTG 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1950R-1.in silico 421 CCTTCATTGTTGAGCTACTAAAGCAGCTGGGCGAGACTGGCCAATTGATGGCCATTTGTG 479
1950R-1.IR_full 481 ACAAGGCCAAGGATCGGTC 498
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1950R-1.in silico 481 ACAAGGCCAAGGATCGGTC 498
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
2 |
NM_132553.1 |
CG1950-RA (CG1950), mRNA |
| 0 |
0 |
9 |
30 |
43 |
NM_079279.2 |
CG3431-RA (Uch-L3), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_165009.1 |
CG31763-RA (CG31763), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168856.1 |
CG18803-RB, transcript variant B (Psn), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079460.2 |
CG18803-RA, transcript variant A (Psn), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_143428.2 |
CG14514-RA (Brd8), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_134689.1 |
CG2794-RA (CG2794), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078772.2 |
CG13772-RA (neuroligin), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165154.1 |
CG31818-RA (CG31818), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057760.4 |
CG12245-RA (gcm), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_130688.2 |
CG14423-RA (CG14423), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_078615.3 |
CG9045-RA, transcript variant A (Myb), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206734.1 |
CG9045-RB, transcript variant B (Myb), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206733.1 |
CG9045-RD, transcript variant D (Myb), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206732.1 |
CG9045-RC, transcript variant C (Myb), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206731.1 |
CG9045-RE, transcript variant E (Myb), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_057685.3 |
CG11527-RA (Tig), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137624.1 |
CG9090-RA (CG9090), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_078590.2 |
CG1771-RB, transcript variant B (mew), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_167354.1 |
CG1771-RA, transcript variant A (mew), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_169275.1 |
CG8874-RC, transcript variant C (Fps85D), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_169274.1 |
CG8874-RB, transcript variant B (Fps85D), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_079564.3 |
CG8874-RA, transcript variant A (Fps85D), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_132068.1 |
CG15899-RB (Ca-alpha1T), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_058067.2 |
CG6147-RA (Tsc1), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_135772.1 |
CG9426-RA (CG9426), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_139392.1 |
CG7991-RA (CG7991), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_057601.2 |
CG2851-RA (Gsc), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_136557.2 |
CG8635-RA (CG8635), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_080707.2 |
CG3430-RA (CG3430), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
|
Caspase-3 signal
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme03082
:
ATP-dependent chromatin remodeling
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