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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

1891R-3

Symbol

sax

Full Name

saxophone

CG No

CG1891

Old CG No

CG1891

Synonyms

sax, CG1891, Sax, Bkr43E, Brk43E, STK-B

Accession No (Link to NCBI)

NM_078928.2

Inserted Chr.

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

abnormal wing vein

Map Viewer

Reference
[Please submit your publication]

Ostalé CM, Esteban N, López-Varea A, de Celis JF. Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing. G3 (Bethesda) (2021) 11(12) [ PubMed ID = 34599799 ] [ RRC reference ]

Cho Y, Lai CM, Lin KY, Hsu HJ. A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development. G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGCCAGAGCCGAAATCAGCGA

Primer Seq. 3'

aatctagaggtaccAAGGACCCAGAACGGCGACGA

Predicted Fragment Size

IR fragment full Seq

0001 TGCCAGAGCC GAAATCAGCG ATCATCTGCG GGAGGACATA CCGGACTTAG ACATGGAGCT 
0061 CTCCTCCGCC TCATCCGCCC ATCTCAATGG CAAGGAGCTA CCGGCCGTGC CACAGAACTC 
0121 GGTGCAGACG CATCCCAGAT ACAAATGCTA CTCCTGCGAN GCCGCCCTGT CGGGATCCCT 
0181 ATGAGTTTAC CCACACCTGC CAGAATGCGA TTCAGTGCTG GAAGTCGCGC ACACGCGATG 
0241 CCGATGGTCA GGTGCAGGAG TCACGCGGCT GCAGCACCTC GCCGGATCAA CTGCCCATGA 
0301 TCTGCAGCCA GAACTCCTTG AAGATCAACG GGCCCAGTAA ACGCAATACG GGGAAGTTCG 
0361 TGAATGTGGT CTGCTGTGCC GGGTGATTAC TGCAATGAGG GAGACTTTCC GGNAACTGCT 
0421 GCCCCTTCGA CAGCAATGAT GTGACGGTGA TAACCGCGGA TACCAGTAGC ATAAGCAAGA 
0481 TgCTCGTCGC CATTCTGGGT CCTT

in silico PCR Fragment

0001 TGCCAGAGCC GAAATCAGCG ATCATCTGCG GGAGGACATA CCGGACTTAG ACATGGAGCT 
0061 CTCCTCCGCC TCATCCGCCC ATCTCAATGG CAAGGAGCTA CCGGCCGTGC CACAGAACTC 
0121 GGTGCAGACG CATCCCAGAT ACAAATGCTA CTCCTGCGA- GCCGCCCTGT CGGGATCCCT 
0181 ATGAGTTTAC CCACACCTGC CAGAATGCGA TTCAGTGCTG GAAGTCGCGC ACACGCGATG 
0241 CCGATGGTCA GGTGCAGGAG TCACGCGGCT GCAGCACCTC GCCGGATCAA CTGCCCATGA 
0301 TCTGCAGCCA GAACTCCTTG AAGATCAACG GGCCCAGTAA ACGCAATACG GGGAAGTTCG 
0361 TGAATGTGGT CTGCTGTGCC -GGTGATTAC TGCAATGAGG GAGACTTTCC GG-AACTGCT 
0421 G-CCCTTCGA CAGCAATGAT GTGACGGTGA TAACCGCGGA TACCAGTAGC ATAAGCAAGA 
0481 TGCTCGTCGC CGTTCTGGGT CCTT

Assemble Data

<PRE>

1891R-3.IR_full       1   TGCCAGAGCCGAAATCAGCGATCATCTGCGGGAGGACATACCGGACTTAGACATGGAGCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1891R-3.in silico     1   TGCCAGAGCCGAAATCAGCGATCATCTGCGGGAGGACATACCGGACTTAGACATGGAGCT 60


1891R-3.IR_full       61  CTCCTCCGCCTCATCCGCCCATCTCAATGGCAAGGAGCTACCGGCCGTGCCACAGAACTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1891R-3.in silico     61  CTCCTCCGCCTCATCCGCCCATCTCAATGGCAAGGAGCTACCGGCCGTGCCACAGAACTC 120


1891R-3.IR_full       121 GGTGCAGACGCATCCCAGATACAAATGCTACTCCTGCGA<span class="snp"><tt>N</tt></span>GCCGCCCTGTCGGGATCCCT 180
                          |||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||
1891R-3.in silico     121 GGTGCAGACGCATCCCAGATACAAATGCTACTCCTGCGA<span class="snp"><tt>-</tt></span>GCCGCCCTGTCGGGATCCCT 180


1891R-3.IR_full       181 ATGAGTTTACCCACACCTGCCAGAATGCGATTCAGTGCTGGAAGTCGCGCACACGCGATG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1891R-3.in silico     181 ATGAGTTTACCCACACCTGCCAGAATGCGATTCAGTGCTGGAAGTCGCGCACACGCGATG 240


1891R-3.IR_full       241 CCGATGGTCAGGTGCAGGAGTCACGCGGCTGCAGCACCTCGCCGGATCAACTGCCCATGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1891R-3.in silico     241 CCGATGGTCAGGTGCAGGAGTCACGCGGCTGCAGCACCTCGCCGGATCAACTGCCCATGA 300


1891R-3.IR_full       301 TCTGCAGCCAGAACTCCTTGAAGATCAACGGGCCCAGTAAACGCAATACGGGGAAGTTCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1891R-3.in silico     301 TCTGCAGCCAGAACTCCTTGAAGATCAACGGGCCCAGTAAACGCAATACGGGGAAGTTCG 360


1891R-3.IR_full       361 TGAATGTGGTCTGCTGTGCC<span class="snp"><tt>G</tt></span>GGTGATTACTGCAATGAGGGAGACTTTCCGG<span class="snp"><tt>N</tt></span>AACTGCT 420
                          ||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
1891R-3.in silico     361 TGAATGTGGTCTGCTGTGCC<span class="snp"><tt>-</tt></span>GGTGATTACTGCAATGAGGGAGACTTTCCGG<span class="snp"><tt>-</tt></span>AACTGCT 420


1891R-3.IR_full       421 G<span class="snp"><tt>C</tt></span>CCCTTCGACAGCAATGATGTGACGGTGATAACCGCGGATACCAGTAGCATAAGCAAGA 480
                          |<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1891R-3.in silico     421 G<span class="snp"><tt>-</tt></span>CCCTTCGACAGCAATGATGTGACGGTGATAACCGCGGATACCAGTAGCATAAGCAAGA 480


1891R-3.IR_full       481 TGCTCGTCGCC<span class="snp"><tt>A</tt></span>TTCTGGGTCCTT 504
                          |||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
1891R-3.in silico     481 TGCTCGTCGCC<span class="snp"><tt>G</tt></span>TTCTGGGTCCTT 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_078928.2	CG1891-RA, transcript variant A (sax), mRNA
98.34	474	2	1	1	NM_165559.1	CG1891-RB, transcript variant B (sax), mRNA
0	0	2	4	3	NM_135854.2	CG16876-RA (CG16876), mRNA
0	0	2	3	2	NM_168288.1	CG32029-RA (CG32029), mRNA
0	0	2	1	1	NM_001042973.1	CG40129-RA (Gprk1), mRNA
0	0	1	3	9	NM_175960.3	CG33196-RB (dp), mRNA
0	0	1	3	6	NM_176254.1	CG5403-RB, transcript variant B (retn), mRNA
0	0	1	3	6	NM_057516.3	CG5403-RA, transcript variant A (retn), mRNA
0	0	1	3	6	NM_206697.1	CG1522-RB, transcript variant B (cac), mRNA
0	0	1	3	6	NM_206693.1	CG1522-RF, transcript variant F (cac), mRNA
0	0	1	3	6	NM_001014732.1	CG1522-RJ, transcript variant J (cac), mRNA
0	0	1	3	6	NM_001014735.1	CG1522-RG, transcript variant G (cac), mRNA
0	0	1	3	6	NM_206695.1	CG1522-RD, transcript variant D (cac), mRNA
0	0	1	3	6	NM_206694.1	CG1522-RE, transcript variant E (cac), mRNA
0	0	1	3	6	NM_001014733.1	CG1522-RI, transcript variant I (cac), mRNA
0	0	1	3	6	NM_001014734.1	CG1522-RH, transcript variant H (cac), mRNA
0	0	1	3	6	NM_078578.2	CG1522-RA, transcript variant A (cac), mRNA
0	0	1	3	6	NM_167340.1	CG32648-RA (Pde9), mRNA
0	0	1	3	6	NM_206696.1	CG1522-RC, transcript variant C (cac), mRNA
0	0	1	3	3	NM_176469.1	CG3359-RL, transcript variant L (mfas), mRNA
0	0	1	3	3	NM_079600.2	CG3359-RC, transcript variant C (mfas), mRNA
0	0	1	3	3	NM_176474.1	CG3359-RO, transcript variant O (mfas), mRNA
0	0	1	3	3	NM_176470.1	CG3359-RP, transcript variant P (mfas), mRNA
0	0	1	3	3	NM_176472.1	CG3359-RK, transcript variant K (mfas), mRNA
0	0	1	3	3	NM_176466.1	CG3359-RH, transcript variant H (mfas), mRNA
0	0	1	3	3	NM_176475.1	CG3359-RF, transcript variant F (mfas), mRNA
0	0	1	3	3	NM_176477.1	CG3359-RM, transcript variant M (mfas), mRNA
0	0	1	3	3	NM_176473.1	CG3359-RE, transcript variant E (mfas), mRNA
0	0	1	3	3	NM_176468.1	CG3359-RD, transcript variant D (mfas), mRNA
0	0	1	3	3	NM_176471.1	CG3359-RI, transcript variant I (mfas), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme04350 : TGF-beta signaling pathway

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