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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1765R-4 
 Symbol EcR  Full Name Ecdysone receptor 
 CG No CG1765  Old CG No CG1765 
 Synonyms Ecr, EcRB1, EcR-B1, ecr, DmEcR, EcRB-1, CG1765, EcR-A, NR1H1, DEcR, snt, ECR, dECR, lie, EcdR, CG8347, ms(2)42A, ms(2)06410, Dhr23, anon-WO0229075.1, EcR, EcR-B 
 Accession No (Link to NCBI) NM_165465.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Gancz D, Gilboa L.
Insulin and Target of rapamycin signaling orchestrate the development of ovarian niche-stem cell units in Drosophila.
Development (2013) 140(20) 4145-54 [ PubMed ID = 24026119 ] [ RRC reference ]

Denton D, Xu T, Dayan S, Nicolson S, Kumar S.
Dpp regulates autophagy-dependent midgut removal and signals to block ecdysone production.
Cell Death Differ. (2018) [ PubMed ID = 29959404 ] [ RRC reference ]

Gancz D, Lengil T, Gilboa L.
Coordinated regulation of niche and stem cell precursors by hormonal signaling.
PLoS Biol. (2011) 9(11) e1001202 [ PubMed ID = 22131903 ] [ RRC reference ]

Sinenko SA, Hung T, Moroz T, Tran QM, Sidhu S, Cheney MD, Speck NA, Banerjee U.
Genetic manipulation of AML1-ETO-induced expansion of hematopoietic precursors in a Drosophila model.
Blood (2010) 116(22) 4612-20 [ PubMed ID = 20688956 ] [ RRC reference ]

Dourlen P, Fernandez-Gomez FJ, Dupont C, Grenier-Boley B, Bellenguez C, Obriot H, Caillierez R, Sottejeau Y, Chapuis J, Bretteville A, Abdelfettah F, Delay C, Malmanche N, Soininen H, Hiltunen M, Galas MC, Amouyel P, Sergeant N, Buée L, Lambert JC, Dermaut B.
Functional screening of Alzheimer risk loci identifies PTK2B as an in vivo modulator and early marker of Tau pathology.
Mol. Psychiatry (2017) 22(6) 874-883 [ PubMed ID = 27113998 ] [ RRC reference ]

Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev. Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]

Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev. Biol. (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCCGGGTGCCACTAATCTGGGA 
 Primer Seq. 3'
aatctagaggtaccGCGTTGTAGTGGTAGCCGGAG 
 Predicted Fragment Size
491 
 IR fragment full Seq
0001 CCGGGTGCCA CTAATCTGGG AGCGTTGNCC AACGGGATGC TCAATggGGG CTTCAATGGA 
0061 ATGCAGCAAC AGATTCAGAA TGGCCACGGC CTCATCAACT CCACAACGCC CTCAACGCCG 
0121 ACCACCCCGC TCCACCTTCA GCAGAACCTG GGGGGCGCGG GCGGCGGCGG TNATCGGGGG 
0181 AATGGGTATT CTTCACCACG CGAATGGCAC CCCAAATGGC CTTATCGGAG TTGTGGGAGG 
0241 CGGCGGCGGA GTAGGTCTTG GAGTAGGCGG AGGCGGAGTG GGAGGCCTGG GAATGCAGCA 
0301 CACACCCCGA AGCGATTCGG TGAATTCTAT ATCTTCAGGT CGCGATGATC TCTCGCCTTC 
0361 GAGCAGCTTG AACGGATACT CGGCGAACGA AAGCTGNGAT GCGAAGAAGA GCAAGAAGGG 
0421 ACCTGCGCCA CGGGTGCAAG AGGAGCTGTG CCTGGTTTGC GGCGANCAgg gCNTCCGGCT 
0481 ACCACTANAA NGC 
 in silico PCR Fragment
0001 CCGGGTGCCA CTAATCTGGG AGCGTTGGCC AACGGGATGC TCAATGGGGG CTTCAATGGA 
0061 ATGCAGCAAC AGATTCAGAA TGGCCACGGC CTCATCAACT CCACAACGCC CTCAACGCCG 
0121 ACCACCCCGC TCCACCTTCA GCAGAACCTG GGGGGCGCGG GCGGCGGCGG T-ATCGGGGG 
0181 AATGGGTATT CTTCACCACG CGAATGGCAC CCCAAATGGC CTTATCGGAG TTGTGGGAGG 
0241 CGGCGGCGGA GTAGGTCTTG GAGTAGGCGG AGGCGGAGTG GGAGGCCTGG GAATGCAGCA 
0301 CACACCCCGA AGCGATTCGG TGAATTCTAT ATCTTCAGGT CGCGATGATC TCTCGCCTTC 
0361 GAGCAGCTTG AACGGATACT CGGCGAACGA AAGCTGCGAT GCGAAGAAGA GCAAGAAGGG 
0421 ACCTGCGCCA CGGGTGCAAG AGGAGCTGTG CCTGGTTTGC GGCGA-CAGG GCCTCCGGCT 
0481 ACCACTACAA CGC 
 Assemble Data

1765R-4.IR_full       1   CCGGGTGCCACTAATCTGGGAGCGTTGNCCAACGGGATGCTCAATGGGGGCTTCAATGGA 60
                          ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
1765R-4.in silico     1   CCGGGTGCCACTAATCTGGGAGCGTTGGCCAACGGGATGCTCAATGGGGGCTTCAATGGA 60


1765R-4.IR_full       61  ATGCAGCAACAGATTCAGAATGGCCACGGCCTCATCAACTCCACAACGCCCTCAACGCCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1765R-4.in silico     61  ATGCAGCAACAGATTCAGAATGGCCACGGCCTCATCAACTCCACAACGCCCTCAACGCCG 120


1765R-4.IR_full       121 ACCACCCCGCTCCACCTTCAGCAGAACCTGGGGGGCGCGGGCGGCGGCGGTNATCGGGGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
1765R-4.in silico     121 ACCACCCCGCTCCACCTTCAGCAGAACCTGGGGGGCGCGGGCGGCGGCGGT-ATCGGGGG 180


1765R-4.IR_full       181 AATGGGTATTCTTCACCACGCGAATGGCACCCCAAATGGCCTTATCGGAGTTGTGGGAGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1765R-4.in silico     181 AATGGGTATTCTTCACCACGCGAATGGCACCCCAAATGGCCTTATCGGAGTTGTGGGAGG 240


1765R-4.IR_full       241 CGGCGGCGGAGTAGGTCTTGGAGTAGGCGGAGGCGGAGTGGGAGGCCTGGGAATGCAGCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1765R-4.in silico     241 CGGCGGCGGAGTAGGTCTTGGAGTAGGCGGAGGCGGAGTGGGAGGCCTGGGAATGCAGCA 300


1765R-4.IR_full       301 CACACCCCGAAGCGATTCGGTGAATTCTATATCTTCAGGTCGCGATGATCTCTCGCCTTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1765R-4.in silico     301 CACACCCCGAAGCGATTCGGTGAATTCTATATCTTCAGGTCGCGATGATCTCTCGCCTTC 360


1765R-4.IR_full       361 GAGCAGCTTGAACGGATACTCGGCGAACGAAAGCTGNGATGCGAAGAAGAGCAAGAAGGG 420
                          |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
1765R-4.in silico     361 GAGCAGCTTGAACGGATACTCGGCGAACGAAAGCTGCGATGCGAAGAAGAGCAAGAAGGG 420


1765R-4.IR_full       421 ACCTGCGCCACGGGTGCAAGAGGAGCTGTGCCTGGTTTGCGGCGANCAGGGCNTCCGGCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||| |||||| |||||||
1765R-4.in silico     421 ACCTGCGCCACGGGTGCAAGAGGAGCTGTGCCTGGTTTGCGGCGA-CAGGGCCTCCGGCT 480


1765R-4.IR_full       481 ACCACTANAANGC 493
                          ||||||| || ||
1765R-4.in silico     481 ACCACTACAACGC 493

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   473  NM_165465.1  CG1765-RB, transcript variant B (EcR), mRNA 
29.38   139  NM_165461.1  CG1765-RA, transcript variant A (EcR), mRNA 
29.38   139  NM_165463.1  CG1765-RE, transcript variant E (EcR), mRNA 
29.38   139  NM_165462.1  CG1765-RD, transcript variant D (EcR), mRNA 
28.96   137  NM_165464.1  CG1765-RC, transcript variant C (EcR), mRNA 
0.21   NM_166480.1  CG17950-RB, transcript variant B (HmgD), mRNA 
0.21   NM_166479.1  CG17950-RA, transcript variant A (HmgD), mRNA 
0   16  28  NM_167394.1  CG32603-RA (CG32603), mRNA 
0   11  NM_167685.1  CG12701-RB, transcript variant B (CG12701), mRNA 
0   11  NM_134512.4  CG12701-RA, transcript variant A (CG12701), mRNA 
0   NM_141965.1  CG14395-RA (CG14395), mRNA 
0   NM_001043260.1  CG34157-RD, transcript variant D (Dys), mRNA 
0   10  NM_079747.2  CG5394-RA, transcript variant A (Aats-glupro), mRNA 
0   NM_170103.1  CG5394-RB, transcript variant B (Aats-glupro), mRNA 
0   11  NM_079818.2  CG10002-RA (fkh), mRNA 
0   11  NM_166498.1  CG13499-RC, transcript variant C (CG13499), mRNA 
0   11  NM_166497.1  CG13499-RA, transcript variant A (CG13499), mRNA 
0   11  NM_166499.1  CG13499-RD, transcript variant D (CG13499), mRNA 
0   11  NM_137784.1  CG13499-RB, transcript variant B (CG13499), mRNA 
0   NM_001042810.1  CG10952-RB, transcript variant B (eag), mRNA 
0   NM_078603.2  CG10952-RA, transcript variant A (eag), mRNA 
0   NM_078725.2  CG4276-RD, transcript variant D (aru), mRNA 
0   NM_164395.1  CG4276-RC, transcript variant C (aru), mRNA 
0   NM_164393.1  CG4276-RA, transcript variant A (aru), mRNA 
0   NM_141104.1  CG14564-RA (CG14564), mRNA 
0   NM_134550.1  CG1695-RA, transcript variant A (CG1695), mRNA 
0   21  38  NM_137950.1  CG9815-RA (CG9815), mRNA 
0   16  NM_143056.1  CG17462-RA (CG17462), mRNA 
0   11  NM_078689.2  CG14228-RA (Mer), mRNA 
0   NM_137194.1  CG8179-RA (CG8179), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
DC
++ 
DM
VM
 
VB
 
Dcompartment   Penetrance
     

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