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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
17520R-2 |
| Symbol |
CkIIalpha |
Full Name |
Casein kinase II alpha subunit |
| CG No |
CG17520 |
Old CG No |
CG17520 |
| Synonyms |
CK2alpha, CKII, CK2, DmCK2alpha, CKIIalfa, CKIIalpha, dCKII, CK2a, DmCKIIalpha, CK-II, CKII-alpha, Tik, CKIIa, CG17520, CK II, CK2 alpha, CkII, CK-II alpha, CK-2, CkIIalpha, Cask-II-a, anon-WO02059370.53, Casein kinase II, casein kinase 2, dCK2alpha |
| Accession No (Link to NCBI) |
NM_168985.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Chatterjee A, Lamaze A, De J, Mena W, Chélot E, Martin B, Hardin P, Kadener S, Emery P, Rouyer F.
Reconfiguration of a Multi-oscillator Network by Light in the Drosophila Circadian Clock.
Curr Biol (2018) 28(13) 2007-2017.e4
[
PubMed ID = 29910074
]
[
RRC reference
]
Szabó A, Papin C, Zorn D, Ponien P, Weber F, Raabe T, Rouyer F.
The CK2 kinase stabilizes CLOCK and represses its activity in the Drosophila circadian oscillator.
PLoS Biol (2013) 11(8) e1001645
[
PubMed ID = 24013921
]
[
RRC reference
]
Hu L, Huang H, Li J, Yin MX, Lu Y, Wu W, Zeng R, Jiang J, Zhao Y, Zhang L.
Drosophila casein kinase 2 (CK2) promotes warts protein to suppress Yorkie protein activity for growth control.
J Biol Chem (2014) 289(48) 33598-607
[
PubMed ID = 25320084
]
[
RRC reference
]
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Hosono C, Matsuda R, Adryan B, Samakovlis C.
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
Nat Cell Biol (2015) 17(12) 1569-76
[
PubMed ID = 26551273
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAGATGTCAATGCGCACAAAC |
| Primer Seq. 3' |
aatctagaggtaccGGCAAGTCCCCAATCTATAAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CAGATGTCAA TGCGCACAAA CCGGATGAAT ATTGGGACTA TGAAAATTAT GTGGTTGATT
0061 GGGGCAATCA AGACGATTAT CAGTTGGTCC GTAAATTAGG CCGTGGAAAG TATTCTGAGG
0121 TCTTCGAGGC CATTAATATT ACGACCACGG AAAAGTGCGT TGTTAAAATT CTGAAACCTG
0181 TTAAAAAAAA GAAGATAAAG CGTGAAATCA AAATTTTGGA GAACTTGCGT GGAGGAACTA
0241 ATATAATAAC ACTTTTAGCC GTTGTCAAGG ACCCAGTTTC TCGAACACCA GCGTTGATTT
0301 TTGAGCACGT CAACAACACA GATTTCAAGC AACTTTACCA AACATTAACT GATTATGAGA
0361 TTCGTTACTA CTTGTTTGAG CTTCTTAAGG CACTTGACTA CTGCCACAGC ATGGGNAATA
0421 ATGCATCGTG ATGTAAAGCC CCACAATGTT ATGATAGATC ACGAAAATCG AAAATTGCGC
0481 CTTATAGATT GGGGAcTTNC c |
| in silico PCR Fragment |
0001 CAGATGTCAA TGCGCACAAA CCGGATGAAT ATTGGGACTA TGAAAATTAT GTGGTTGATT
0061 GGGGCAATCA AGACGATTAT CAGTTGGTCC GTAAATTAGG CCGTGGAAAG TATTCTGAGG
0121 TCTTCGAGGC CATTAATATT ACGACCACGG AAAAGTGCGT TGTTAAAATT CTGAAACCTG
0181 TTAAAAAAAA GAAGATAAAG CGTGAAATCA AAATTTTGGA GAACTTGCGT GGAGGAACTA
0241 ATATAATAAC ACTTTTAGCC GTTGTCAAGG ACCCAGTTTC TCGAACACCA GCGTTGATTT
0301 TTGAGCACGT CAACAACACA GATTTCAAGC AACTTTACCA AACATTAACT GATTATGAGA
0361 TTCGTTACTA CTTGTTTGAG CTTCTTAAGG CACTTGACTA CTGCCACAGC ATGGG-AATA
0421 ATGCATCGTG ATGTAAAGCC CCACAATGTT ATGATAGATC ACGAAAATCG AAAATTGCGC
0481 CTTATAGATT GGGGACTTGC C |
| Assemble Data |
17520R-2.IR_full 1 CAGATGTCAATGCGCACAAACCGGATGAATATTGGGACTATGAAAATTATGTGGTTGATT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17520R-2.in silico 1 CAGATGTCAATGCGCACAAACCGGATGAATATTGGGACTATGAAAATTATGTGGTTGATT 60
17520R-2.IR_full 61 GGGGCAATCAAGACGATTATCAGTTGGTCCGTAAATTAGGCCGTGGAAAGTATTCTGAGG 120
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17520R-2.in silico 61 GGGGCAATCAAGACGATTATCAGTTGGTCCGTAAATTAGGCCGTGGAAAGTATTCTGAGG 120
17520R-2.IR_full 121 TCTTCGAGGCCATTAATATTACGACCACGGAAAAGTGCGTTGTTAAAATTCTGAAACCTG 180
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17520R-2.in silico 121 TCTTCGAGGCCATTAATATTACGACCACGGAAAAGTGCGTTGTTAAAATTCTGAAACCTG 180
17520R-2.IR_full 181 TTAAAAAAAAGAAGATAAAGCGTGAAATCAAAATTTTGGAGAACTTGCGTGGAGGAACTA 240
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17520R-2.in silico 181 TTAAAAAAAAGAAGATAAAGCGTGAAATCAAAATTTTGGAGAACTTGCGTGGAGGAACTA 240
17520R-2.IR_full 241 ATATAATAACACTTTTAGCCGTTGTCAAGGACCCAGTTTCTCGAACACCAGCGTTGATTT 300
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17520R-2.in silico 241 ATATAATAACACTTTTAGCCGTTGTCAAGGACCCAGTTTCTCGAACACCAGCGTTGATTT 300
17520R-2.IR_full 301 TTGAGCACGTCAACAACACAGATTTCAAGCAACTTTACCAAACATTAACTGATTATGAGA 360
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17520R-2.in silico 301 TTGAGCACGTCAACAACACAGATTTCAAGCAACTTTACCAAACATTAACTGATTATGAGA 360
17520R-2.IR_full 361 TTCGTTACTACTTGTTTGAGCTTCTTAAGGCACTTGACTACTGCCACAGCATGGGNAATA 420
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17520R-2.in silico 361 TTCGTTACTACTTGTTTGAGCTTCTTAAGGCACTTGACTACTGCCACAGCATGGG-AATA 420
17520R-2.IR_full 421 ATGCATCGTGATGTAAAGCCCCACAATGTTATGATAGATCACGAAAATCGAAAATTGCGC 480
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17520R-2.in silico 421 ATGCATCGTGATGTAAAGCCCCACAATGTTATGATAGATCACGAAAATCGAAAATTGCGC 480
17520R-2.IR_full 481 CTTATAGATTGGGGACTTNCC 501
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17520R-2.in silico 481 CTTATAGATTGGGGACTTGCC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_001043159.1 |
CG17520-RD, transcript variant D (CkIIalpha), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_168985.1 |
CG17520-RA, transcript variant A (CkIIalpha), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_168986.1 |
CG17520-RC, transcript variant C (CkIIalpha), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_080179.4 |
CG17520-RB, transcript variant B (CkIIalpha), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_164364.2 |
CG4822-RB, transcript variant B (CG4822), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_168683.1 |
CG9705-RB, transcript variant B (CG9705), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_140663.2 |
CG9705-RA, transcript variant A (CG9705), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_001042832.1 |
CG41476-RA (CG41476), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_001042998.1 |
CG17528-RC, transcript variant C (CG17528), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_001042997.1 |
CG17528-RD, transcript variant D (CG17528), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_001042996.1 |
CG17528-RB, transcript variant B (CG17528), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_001042999.1 |
CG17528-RA, transcript variant A (CG17528), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_080096.2 |
CG7869-RA (SuUR), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142241.1 |
CG4560-RB, transcript variant B (Arpc3A), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_169675.1 |
CG4560-RA, transcript variant A (Arpc3A), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_144346.2 |
CG6264-RA (Best1), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_132995.1 |
CG12991-RA, transcript variant A (CG12991), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167571.1 |
CG12991-RB, transcript variant B (CG12991), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136689.1 |
CG30007-RB, transcript variant B (CG30007), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165719.1 |
CG30007-RA, transcript variant A (CG30007), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078491.2 |
CG4193-RA (dhd), mRNA |
| 0 |
0 |
0 |
85 |
323 |
NM_168226.1 |
CG32377-RA (CG32377), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_168504.1 |
CG32111-RA (CG32111), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001031890.1 |
CG14425-RA, transcript variant A (CG14425), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001031891.1 |
CG18350-RO, transcript variant O (Sxl), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001031893.1 |
CG18350-RM, transcript variant M (Sxl), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_167114.2 |
CG18350-RH, transcript variant H (Sxl), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_142963.2 |
CG6173-RA (kal-1), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_166489.2 |
CG13491-RA (Gr58c), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_057597.2 |
CG10719-RA, transcript variant A (brat), mRNA |
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