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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
17299R-1 |
| Symbol |
SNF4Agamma |
Full Name |
SNF4/AMP-activated protein kinase gamma subunit |
| CG No |
CG17299 |
Old CG No |
CG17299 |
| Synonyms |
SNF4Ag, SNF4A, loe, FBgn0025803, SNF4a, CG17299, EP3015b, SNF4, CG5806, anon-WO0257455.21, anon-WO0118547.575, anon-WO0118547.338, SNF4Agamma, SNF4/AMP-activated protein kinase gamma subunit, SNF4/AMP-activated protein kinase gamma, lochrig, loechrig |
| Accession No (Link to NCBI) |
NM_169946.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCATGCAGAACTTTAGCCATCG |
| Primer Seq. 3' |
aatctagaggtaccAGGTGGGTATCAGATCATAGC |
| Predicted Fragment Size |
539 |
| IR fragment full Seq |
0001 CATGCAGAAC TTTAGCCATC GCCAGCATCC CGCCGTGACG ATAACGAGCG CCGACGGCAC
0061 GCAATCAACG GCAAAGAGCA AGTACAAAGA CGGCAGTGCC CATCCGCATC AAGGCAGCGA
0121 CGCGCAGTAT TACCACACGG TGACGGCGGT GCGTCCAAAC TCTTCCCAAC GGTCGCCGAT
0181 GACCAAGGTC ATGGATCTGT TCCGGCATCG ATCCAGCTCG GTTGTCAGCG AAGCCGACAA
0241 ACGCAAAGCG CGCGCGGCGG CGCATCAGCA ACAGTTGGCT GTGCAAAGTG CTCACATGCG
0301 TCGTGCCTCC GCGGATTTGG AGAAACGTCG TGCATCAGTT GGTGCCGCAG GTCGAGGACT
0361 GCGAGGGGAT GGTACTTTGG ATCCACACCA TGCAGCCATC CTCTTCAGAG ACTCACGAGG
0421 GTTGCCTGTC GCTGATCCGT TCCTAGAGAA AGTAAATCTA TCAGATCTGG AAGAGGACGA
0481 CTCACAGATC TTCGTGAAGT TCTTTCGTTT TCACAAGTGC TATGATCTGA TACCCACCT |
| in silico PCR Fragment |
0001 CATGCAGAAC TTTAGCCATC GCCAGCATCC CGCCGTGACG ATAACGAGCG CCGACGGCAC
0061 GCAATCAACG GCAAAGAGCA AGTACAAAGA CGGCAGTGCC CATCCGCATC AAGGCAGCGA
0121 CGCGCAGTAT TACCACACGG TGACGGCGGT GCGTCCAAAC TCTTCCCAAC GGTCGCCGAT
0181 GACCAAGGTC ATGGATCTGT TCCGGCATCG ATCCAGCTCG GTTGTCAGCG AAGCCGACAA
0241 ACGCAAAGCG CGCGCGGCGG CGCATCAGCA ACAGTTGGCT GTGCAAAGTG CTCACATGCG
0301 TCGTGCCTCC GCGGATTTGG AGAAACGTCG TGCATCAGTT GGTGCCGCAG GTCGAGGACT
0361 GCGAGGGGAT GGTACTTTGG ATCCACACCA TGCAGCCATC CTCTTCAGAG ACTCACGAGG
0421 GTTGCCTGTC GCTGATCCGT TCCTAGAGAA AGTAAATCTA TCAGATCTGG AAGAGGACGA
0481 CTCACAGATC TTCGTGAAGT TCTTTCGTTT TCACAAGTGC TATGATCTGA TACCCACCT |
| Assemble Data |
17299R-1.IR_full 1 CATGCAGAACTTTAGCCATCGCCAGCATCCCGCCGTGACGATAACGAGCGCCGACGGCAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17299R-1.in silico 1 CATGCAGAACTTTAGCCATCGCCAGCATCCCGCCGTGACGATAACGAGCGCCGACGGCAC 60
17299R-1.IR_full 61 GCAATCAACGGCAAAGAGCAAGTACAAAGACGGCAGTGCCCATCCGCATCAAGGCAGCGA 120
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17299R-1.in silico 61 GCAATCAACGGCAAAGAGCAAGTACAAAGACGGCAGTGCCCATCCGCATCAAGGCAGCGA 120
17299R-1.IR_full 121 CGCGCAGTATTACCACACGGTGACGGCGGTGCGTCCAAACTCTTCCCAACGGTCGCCGAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17299R-1.in silico 121 CGCGCAGTATTACCACACGGTGACGGCGGTGCGTCCAAACTCTTCCCAACGGTCGCCGAT 180
17299R-1.IR_full 181 GACCAAGGTCATGGATCTGTTCCGGCATCGATCCAGCTCGGTTGTCAGCGAAGCCGACAA 240
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17299R-1.in silico 181 GACCAAGGTCATGGATCTGTTCCGGCATCGATCCAGCTCGGTTGTCAGCGAAGCCGACAA 240
17299R-1.IR_full 241 ACGCAAAGCGCGCGCGGCGGCGCATCAGCAACAGTTGGCTGTGCAAAGTGCTCACATGCG 300
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17299R-1.in silico 241 ACGCAAAGCGCGCGCGGCGGCGCATCAGCAACAGTTGGCTGTGCAAAGTGCTCACATGCG 300
17299R-1.IR_full 301 TCGTGCCTCCGCGGATTTGGAGAAACGTCGTGCATCAGTTGGTGCCGCAGGTCGAGGACT 360
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17299R-1.in silico 301 TCGTGCCTCCGCGGATTTGGAGAAACGTCGTGCATCAGTTGGTGCCGCAGGTCGAGGACT 360
17299R-1.IR_full 361 GCGAGGGGATGGTACTTTGGATCCACACCATGCAGCCATCCTCTTCAGAGACTCACGAGG 420
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17299R-1.in silico 361 GCGAGGGGATGGTACTTTGGATCCACACCATGCAGCCATCCTCTTCAGAGACTCACGAGG 420
17299R-1.IR_full 421 GTTGCCTGTCGCTGATCCGTTCCTAGAGAAAGTAAATCTATCAGATCTGGAAGAGGACGA 480
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17299R-1.in silico 421 GTTGCCTGTCGCTGATCCGTTCCTAGAGAAAGTAAATCTATCAGATCTGGAAGAGGACGA 480
17299R-1.IR_full 481 CTCACAGATCTTCGTGAAGTTCTTTCGTTTTCACAAGTGCTATGATCTGATACCCACCT 539
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17299R-1.in silico 481 CTCACAGATCTTCGTGAAGTTCTTTCGTTTTCACAAGTGCTATGATCTGATACCCACCT 539
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
521 |
0 |
0 |
2 |
NM_169946.1 |
CG17299-RA, transcript variant A (SNF4Agamma), mRNA |
| 100 |
521 |
0 |
0 |
2 |
NM_080509.2 |
CG17299-RB, transcript variant B (SNF4Agamma), mRNA |
| 100 |
521 |
0 |
0 |
1 |
NM_169947.1 |
CG17299-RF, transcript variant F (SNF4Agamma), mRNA |
| 44.91 |
234 |
3 |
1 |
2 |
NM_169948.1 |
CG17299-RC, transcript variant C (SNF4Agamma), mRNA |
| 44.91 |
234 |
3 |
1 |
2 |
NM_169949.1 |
CG17299-RE, transcript variant E (SNF4Agamma), mRNA |
| 44.91 |
234 |
3 |
1 |
2 |
NM_001043273.1 |
CG17299-RJ, transcript variant J (SNF4Agamma), mRNA |
| 44.91 |
234 |
3 |
1 |
2 |
NM_001043272.1 |
CG17299-RK, transcript variant K (SNF4Agamma), mRNA |
| 19.19 |
100 |
2 |
1 |
4 |
NM_169950.1 |
CG17299-RG, transcript variant G (SNF4Agamma), mRNA |
| 19.19 |
100 |
2 |
1 |
4 |
NM_001043271.1 |
CG17299-RL, transcript variant L (SNF4Agamma), mRNA |
| 9.98 |
52 |
1 |
1 |
1 |
NM_001043274.1 |
CG17299-RI, transcript variant I (SNF4Agamma), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_143701.2 |
CG6698-RA (NtR), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_167844.1 |
CG32479-RA (CG32479), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_140263.1 |
CG14127-RA (CG14127), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_079818.2 |
CG10002-RA (fkh), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_165508.1 |
CG11084-RA, transcript variant A (pk), mRNA |
| 0 |
0 |
1 |
5 |
11 |
NM_206552.1 |
CG31140-RC, transcript variant C (CG31140), mRNA |
| 0 |
0 |
1 |
5 |
11 |
NM_206553.1 |
CG31140-RB, transcript variant B (CG31140), mRNA |
| 0 |
0 |
1 |
5 |
11 |
NM_142942.3 |
CG31140-RA, transcript variant A (CG31140), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_168224.1 |
CG14837-RA, transcript variant A (CG14837), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_139879.1 |
CG14837-RB, transcript variant B (CG14837), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_169497.1 |
CG8476-RA (CG8476), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140238.1 |
CG14135-RA (CG14135), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165509.1 |
CG11084-RB, transcript variant B (pk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165512.1 |
CG11084-RC, transcript variant C (pk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206768.1 |
CG9132-RA, transcript variant A (CG9132), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206767.1 |
CG9132-RB, transcript variant B (CG9132), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132476.2 |
CG1657-RA (CG1657), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001014503.1 |
CG8715-RD, transcript variant D (lig), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001042891.1 |
CG31761-RE, transcript variant E (bru-2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_142106.1 |
CG14843-RA (CG14843), mRNA |
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