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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
17180R-1 |
| Symbol |
CG17180 |
Full Name |
CG17180 |
| CG No |
CG17180 |
Old CG No |
CG17180 |
| Synonyms |
CG17180 |
| Accession No (Link to NCBI) |
NM_138193.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Boda A, Lőrincz P, Takáts S, Csizmadia T, Tóth S, Kovács AL, Juhász G.
Drosophila Arl8 is a general positive regulator of lysosomal fusion events.
Biochim Biophys Acta Mol Cell Res (2019) 1866(4) 533-544
[
PubMed ID = 30590083
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGCCACCAGGACATTCCCATC |
| Primer Seq. 3' |
aatctagaggtaccTGGGCCACAAAGTGGCGCATC |
| Predicted Fragment Size |
443 |
| IR fragment full Seq |
0001 CGCCACCAGG ACATTCCCAT CCGCCCACGT TACGGTTATG TGGCCGAGGA GAGTGGCCCA
0061 TCGGAAGGGG CAGCCAGGAG TTCTGGATCC GGAGCCACGC CCGCCTCCTC CTACACGGAA
0121 ATTCCGTTCC TGGCTCAGTA TCCGGGAGGC GGTGCCAGAG CACGTGCTCC AGGATCTGAC
0181 GCNCACAGCG CACTGGCCAT GCTGCGATTC CCGGGAGCAC AAAGACAAGA CCCAACGGCG
0241 AGAAANTACC TTAACCTGGA TTCGGGCGAG GATCCCGACG AGGAAGACGA TCCCTTGGAG
0301 GAGGAGGACA ACAGCAACTC GAATAGCAAC TCCAAGGGCA GTTCCGGCGA CATCGAAAGG
0361 CACAGACTGA AGGCAGAGAG CACTGTGCCC TACGAGCTGG ACGGCGAAAC CAGTGACTCC
0421 GACGGGANTG cgcCACTTTG TGGCCCA |
| in silico PCR Fragment |
0001 CGCCACCAGG ACATTCCCAT CCGCCCACGT TACGGTTATG TGGCCGAGGA GAGTGGCCCA
0061 TCGGAAGGGG CAGCCAGGAG TTCTGGATCC GGAGCCACGC CCGCCTCCTC CTACACGGAA
0121 ATTCCGTTCC TGGCTCAGTA TCCGGGA-GC GGTGCCAGAG CACGTGCTCC AGGATCTGAC
0181 GCCCACAGC- CACTGGCCAT GCTGCGATTC CCGGAAGCAC AAAGACAAGA CCCAACGGCG
0241 AGAAA-TACC TTAACCTGGA TTCGGGCGAG GATCCCGACG AGGAAGACGA TCCCTTGGAG
0301 GAGGAGGACA ACAGCAACTC GAATAGCAAC TCTAAGGGCA GTTCCGGCGA CATCGAAAGG
0361 CACAGACTGA AGGCAGAGAG CACTGTGCCC TACGAGCTGG ACGGCGAAAC CAGTGACTCC
0421 GACGGGA-TG CGCCACTTTG TGGCCCA |
| Assemble Data |
17180R-1.IR_full 1 CGCCACCAGGACATTCCCATCCGCCCACGTTACGGTTATGTGGCCGAGGAGAGTGGCCCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17180R-1.in silico 1 CGCCACCAGGACATTCCCATCCGCCCACGTTACGGTTATGTGGCCGAGGAGAGTGGCCCA 60
17180R-1.IR_full 61 TCGGAAGGGGCAGCCAGGAGTTCTGGATCCGGAGCCACGCCCGCCTCCTCCTACACGGAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17180R-1.in silico 61 TCGGAAGGGGCAGCCAGGAGTTCTGGATCCGGAGCCACGCCCGCCTCCTCCTACACGGAA 120
17180R-1.IR_full 121 ATTCCGTTCCTGGCTCAGTATCCGGGAGGCGGTGCCAGAGCACGTGCTCCAGGATCTGAC 180
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
17180R-1.in silico 121 ATTCCGTTCCTGGCTCAGTATCCGGGA-GCGGTGCCAGAGCACGTGCTCCAGGATCTGAC 180
17180R-1.IR_full 181 GCNCACAGCGCACTGGCCATGCTGCGATTCCCGGGAGCACAAAGACAAGACCCAACGGCG 240
|| |||||| |||||||||||||||||||||||| |||||||||||||||||||||||||
17180R-1.in silico 181 GCCCACAGC-CACTGGCCATGCTGCGATTCCCGGAAGCACAAAGACAAGACCCAACGGCG 240
17180R-1.IR_full 241 AGAAANTACCTTAACCTGGATTCGGGCGAGGATCCCGACGAGGAAGACGATCCCTTGGAG 300
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
17180R-1.in silico 241 AGAAA-TACCTTAACCTGGATTCGGGCGAGGATCCCGACGAGGAAGACGATCCCTTGGAG 300
17180R-1.IR_full 301 GAGGAGGACAACAGCAACTCGAATAGCAACTCCAAGGGCAGTTCCGGCGACATCGAAAGG 360
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
17180R-1.in silico 301 GAGGAGGACAACAGCAACTCGAATAGCAACTCTAAGGGCAGTTCCGGCGACATCGAAAGG 360
17180R-1.IR_full 361 CACAGACTGAAGGCAGAGAGCACTGTGCCCTACGAGCTGGACGGCGAAACCAGTGACTCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17180R-1.in silico 361 CACAGACTGAAGGCAGAGAGCACTGTGCCCTACGAGCTGGACGGCGAAACCAGTGACTCC 420
17180R-1.IR_full 421 GACGGGANTGCGCCACTTTGTGGCCCA 447
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17180R-1.in silico 421 GACGGGA-TGCGCCACTTTGTGGCCCA 447
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
425 |
0 |
0 |
0 |
NM_138193.1 |
CG17180-RA (CG17180), mRNA |
| 0.47 |
2 |
2 |
5 |
5 |
NM_167441.2 |
CG9176-RB, transcript variant B (cngl), mRNA |
| 0 |
0 |
3 |
7 |
5 |
NM_130523.2 |
CG11638-RA (CG11638), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_079268.2 |
CG10923-RA (Klp67A), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_133057.2 |
CG6023-RA, transcript variant A (CG6023), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_206780.1 |
CG6023-RB, transcript variant B (CG6023), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_001042799.1 |
CG10966-RB, transcript variant B (rdgA), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_078537.2 |
CG10966-RA, transcript variant A (rdgA), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_143368.1 |
CG9988-RA (CG9988), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_143271.1 |
CG14257-RA (CG14257), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_142001.1 |
CG17319-RA (CG17319), mRNA |
| 0 |
0 |
2 |
2 |
20 |
NM_141384.2 |
CG15188-RA (Osi20), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_134711.1 |
CG15824-RA (CG15824), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_143348.2 |
CG4849-RA (CG4849), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_168877.1 |
CG3263-RD, transcript variant D (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_079465.3 |
CG3263-RB, transcript variant B (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001014597.1 |
CG3263-RF, transcript variant F (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001014598.1 |
CG3263-RE, transcript variant E (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001014594.1 |
CG3263-RI, transcript variant I (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001014593.1 |
CG3263-RJ, transcript variant J (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_168875.2 |
CG3263-RA, transcript variant A (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001014595.1 |
CG3263-RH, transcript variant H (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001014596.1 |
CG3263-RG, transcript variant G (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_168876.4 |
CG3263-RC, transcript variant C (Pka-R1), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_139557.1 |
CG12014-RA (CG12014), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132395.1 |
CG2898-RA (CG2898), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140681.3 |
CG9665-RA (CG9665), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_145873.2 |
CG18659-RA, transcript variant A (CG18659), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136614.4 |
CG18659-RB, transcript variant B (CG18659), mRNA |
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| Wing Disc |
GFP
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Puc
 |
Caspase
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DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| ++ |
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| DC |
 |
| ++ |
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| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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