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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15862R-2 |
| Symbol |
Pka-R2 |
Full Name |
cAMP-dependent protein kinase R2 |
| CG No |
CG15862 |
Old CG No |
CG15862 |
| Synonyms |
pka-RII, Cos1, PKa-R2, PKA, PKA RII, PKA-RII, CG15862, Pka-RII, RII, RII[[DR]], pkA, Cos-1, Cos, Epa, cos1, BcDNA:GM01761, Pka-R2, Epaulette, costal-1, Costal, cAMP-dependent protein kinase R2, Costal-1, cAMP-dependent protein kinase type II |
| Accession No (Link to NCBI) |
NM_078947.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Szlanka T, Lukacsovich T, Bálint É, Virágh E, Szabó K, Hajdu I, Molnár E, Lin YH, Zvara Á, Kelemen-Valkony I, Méhi O, Török I, Hegedűs Z, Kiss B, Ramasz B, Magdalena LM, Puskás L, Mechler BM, Fónagy A, Asztalos Z, Steinbach G, Žurovec M, Boros I, Kiss I.<br> Dominant suppressor genes of p53-induced apoptosis in Drosophila melanogaster.<br> G3 (Bethesda) (2024) 14(9)
[
PubMed ID = 38985658
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTGCAGTTCTCGATCTCCTTT |
| Primer Seq. 3' |
aatctagaggtaccAGTCCAGTGTGATTGTAGGTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTGCAGTTCT CGATCTCCTT TCTGGTGGAA CAACCGCCGG ATGTGATCGA TTACGCTGTG
0061 GAGTACTTCA CCAAACTGCA GTCGGAACGC CCGAGCGTCN TCTCACACGG ATCAAAGCAC
0121 CGATGACCAG CTGAGCGTCN AACTCACAGG ATGCCGATGC GGNAACCACC AGTGATGGCC
0181 AGCTCGCGTC GCAAATCAGT TTTCGCCGAG GCTNTACGAT CCGGAGGCGG ATGACGACGA
0241 TGATGGCGCC ACCGCCGTGN TTCCCCAAGA CAGATGAGCA GCGCGCCCGG CTGGTCGAGT
0301 CGGTGAAGAA CGTCCTCCTC TTCCGATCCC TCGAGAAGGA GCAGATGAAC CAAGTCCTGG
0361 ATGCCATGTT CGAGCGAAAG GTTCAGCCGG GTGACTTTAT CATCCGCCAG GGTGACGACG
0421 GCGATAACTT TTATGTTATT GAATCTGGCG TCTACAAAGT CTATATTAAT GACAAGCACA
0481 TTAACACCTA CAATCACACT GGACT |
| in silico PCR Fragment |
0001 CTGCAGTTCT CGATCTCCTT TCTGGTGGAA CAACCGCCGG ATGTGATCGA TTACGCTGTG
0061 GAGTACTTCA CCAAACTGCA GTCGGAACGC CCGAGCGTC- TCTCACACGG ATCAAAGCAC
0121 CGATGACCAG CTGAGCGTC- AACTCACAGG ATGCCGATGC GG-AACCACC AGTGATGGCC
0181 AGCTCGCGTC GCAAATCAGT TTTCGCCGAG GCC-TACGAT CCGGAGGCGG ATGACGACGA
0241 TGATGGCGCC ACCGCCGTG- TTCCCCAAGA CAGATGAGCA GCGCGCCCGG CTGGTCGAGT
0301 CGGTGAAGAA CGTCCTCCTC TTCCGATCCC TCGAGAAGGA GCAGATGAAC CAAGTCCTGG
0361 ATGCCATGTT CGAGCGAAAG GTTCAGCCGG GTGACTTTAT CATCCGCCAG GGTGACGACG
0421 GCGATAACTT TTATGTTATT GAATCTGGCG TCTACAAAGT CTATATTAAT GACAAGCACA
0481 TTAACACCTA CAATCACACT GGACT |
| Assemble Data |
<PRE>
15862R-2.IR_full 1 CTGCAGTTCTCGATCTCCTTTCTGGTGGAACAACCGCCGGATGTGATCGATTACGCTGTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15862R-2.in silico 1 CTGCAGTTCTCGATCTCCTTTCTGGTGGAACAACCGCCGGATGTGATCGATTACGCTGTG 60
15862R-2.IR_full 61 GAGTACTTCACCAAACTGCAGTCGGAACGCCCGAGCGTC<span class="snp"><tt>N</tt></span>TCTCACACGGATCAAAGCAC 120
|||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||
15862R-2.in silico 61 GAGTACTTCACCAAACTGCAGTCGGAACGCCCGAGCGTC<span class="snp"><tt>-</tt></span>TCTCACACGGATCAAAGCAC 120
15862R-2.IR_full 121 CGATGACCAGCTGAGCGTC<span class="snp"><tt>N</tt></span>AACTCACAGGATGCCGATGCGG<span class="snp"><tt>N</tt></span>AACCACCAGTGATGGCC 180
|||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||
15862R-2.in silico 121 CGATGACCAGCTGAGCGTC<span class="snp"><tt>-</tt></span>AACTCACAGGATGCCGATGCGG<span class="snp"><tt>-</tt></span>AACCACCAGTGATGGCC 180
15862R-2.IR_full 181 AGCTCGCGTCGCAAATCAGTTTTCGCCGAGGC<span class="snp"><tt>T</tt></span><span class="snp"><tt>N</tt></span>TACGATCCGGAGGCGGATGACGACGA 240
||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||||||||||||||||||
15862R-2.in silico 181 AGCTCGCGTCGCAAATCAGTTTTCGCCGAGGC<span class="snp"><tt>C</tt></span><span class="snp"><tt>-</tt></span>TACGATCCGGAGGCGGATGACGACGA 240
15862R-2.IR_full 241 TGATGGCGCCACCGCCGTG<span class="snp"><tt>N</tt></span>TTCCCCAAGACAGATGAGCAGCGCGCCCGGCTGGTCGAGT 300
|||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||
15862R-2.in silico 241 TGATGGCGCCACCGCCGTG<span class="snp"><tt>-</tt></span>TTCCCCAAGACAGATGAGCAGCGCGCCCGGCTGGTCGAGT 300
15862R-2.IR_full 301 CGGTGAAGAACGTCCTCCTCTTCCGATCCCTCGAGAAGGAGCAGATGAACCAAGTCCTGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15862R-2.in silico 301 CGGTGAAGAACGTCCTCCTCTTCCGATCCCTCGAGAAGGAGCAGATGAACCAAGTCCTGG 360
15862R-2.IR_full 361 ATGCCATGTTCGAGCGAAAGGTTCAGCCGGGTGACTTTATCATCCGCCAGGGTGACGACG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15862R-2.in silico 361 ATGCCATGTTCGAGCGAAAGGTTCAGCCGGGTGACTTTATCATCCGCCAGGGTGACGACG 420
15862R-2.IR_full 421 GCGATAACTTTTATGTTATTGAATCTGGCGTCTACAAAGTCTATATTAATGACAAGCACA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15862R-2.in silico 421 GCGATAACTTTTATGTTATTGAATCTGGCGTCTACAAAGTCTATATTAATGACAAGCACA 480
15862R-2.IR_full 481 TTAACACCTACAATCACACTGGACT 505
|||||||||||||||||||||||||
15862R-2.in silico 481 TTAACACCTACAATCACACTGGACT 505
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_165723.2 |
CG15862-RB, transcript variant B (Pka-R2), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_078947.2 |
CG15862-RA, transcript variant A (Pka-R2), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_176117.1 |
CG15862-RC, transcript variant C (Pka-R2), mRNA |
| 0 |
0 |
2 |
8 |
3 |
NM_206469.1 |
CG11870-RD, transcript variant D (CG11870), mRNA |
| 0 |
0 |
2 |
8 |
3 |
NM_169337.2 |
CG11870-RB, transcript variant B (CG11870), mRNA |
| 0 |
0 |
2 |
8 |
3 |
NM_206470.1 |
CG11870-RC, transcript variant C (CG11870), mRNA |
| 0 |
0 |
2 |
8 |
3 |
NM_141734.2 |
CG11870-RA, transcript variant A (CG11870), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_141791.1 |
CG4800-RA (Tctp), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169434.1 |
CG6946-RB, transcript variant B (glo), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_141863.1 |
CG6946-RA, transcript variant A (glo), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169435.1 |
CG6946-RC, transcript variant C (glo), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132066.1 |
CG4320-RA (raptor), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_139413.2 |
CG12024-RA, transcript variant A (CG12024), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167921.1 |
CG12024-RB, transcript variant B (CG12024), mRNA |
| 0 |
0 |
1 |
6 |
18 |
NM_132831.1 |
CG8184-RB (CG8184), mRNA |
| 0 |
0 |
1 |
6 |
13 |
NM_176497.1 |
CG9297-RB, transcript variant B (CG9297), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_132206.1 |
CG2253-RA (Upf2), mRNA |
| 0 |
0 |
1 |
4 |
13 |
NM_132207.2 |
CG1571-RA (CG1571), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_080373.2 |
CG18681-RA (epsilonTry), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001042881.1 |
CG8486-RC, transcript variant C (CG8486), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_164795.2 |
CG8486-RB, transcript variant B (CG8486), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_135344.2 |
CG8486-RA, transcript variant A (CG8486), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137667.3 |
CG13442-RA (CG13442), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140554.1 |
CG12486-RA (CG12486), mRNA |
| 0 |
0 |
1 |
2 |
13 |
NM_001043070.1 |
CG34146-RA (brp), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_166696.1 |
CG9071-RB, transcript variant B (NaCP60E), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_132471.1 |
CG11105-RB, transcript variant B (CG11105), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167277.1 |
CG11105-RA, transcript variant A (CG11105), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079134.5 |
CG9071-RA, transcript variant A (NaCP60E), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137165.1 |
CG12424-RA, transcript variant A (CG12424), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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