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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1560R-1 |
| Symbol |
mys |
Full Name |
myospheroid |
| CG No |
CG1560 |
Old CG No |
CG1560 |
| Synonyms |
betaPS1, PS, betaPS, Mys, l(1)7Db, integrin beta[[PS]], CG1560, betamys, beta[[PS]]-integrin, MAb6G11, CT40473, olfC, BetaPS, b[[PS]], beta[[PS]], beta-PS, bPS, l(1)968, beta[[P]]S, l(1)mys, PSbeta, nj42, l(1)93p, EM28, l(1)G0233, l(1)G0281, l(1)EM28, l(1)DA551, l(1)7Dn, PS[[beta]], mys, beta[[mys]], betaPS Int |
| Accession No (Link to NCBI) |
NM_080054.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Sun T, Song Y, Teng D, Chen Y, Dai J, Ma M, Zhang W, Pastor-Pareja JC.
Atypical laminin spots and pull-generated microtubule-actin projections mediate Drosophila wing adhesion.
Cell Rep (2021) 36(10) 109667
[
PubMed ID = 34496252
]
[
RRC reference
]
Khadilkar RJ, Ho KYL, Venkatesh B, Tanentzapf G.
Integrins Modulate Extracellular Matrix Organization to Control Cell Signaling during Hematopoiesis.
Curr Biol (2020) 30(17) 3316-3329.e5
[
PubMed ID = 32649911
]
[
RRC reference
]
Hunter AC, Petley-Ragan LM, Das M, Auld VJ.
Basigin Associates with Integrin in Order to Regulate Perineurial Glia and Drosophila Nervous System Morphology.
J Neurosci (2020) 40(17) 3360-3373
[
PubMed ID = 32265259
]
[
RRC reference
]
Park SH, Lee CW, Choe KM.
Interplay between integrins and PI4P5K Sktl is crucial for cell polarization and reepithelialisation during Drosophila wound healing.
Sci Rep (2019) 9(1) 16331
[
PubMed ID = 31704968
]
[
RRC reference
]
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Park SH, Lee CW, Lee JH, Park JY, Roshandell M, Brennan CA, Choe KM.
Requirement for and polarized localization of integrin proteins during Drosophila wound closure.
Mol Biol Cell (2018) 29(18) 2137-2147
[
PubMed ID = 29995573
]
[
RRC reference
]
Dai J, Ma M, Feng Z, Pastor-Pareja JC.
Inter-adipocyte Adhesion and Signaling by Collagen IV Intercellular Concentrations in Drosophila.
Curr Biol (2017) 27(18) 2729-2740.e4
[
PubMed ID = 28867208
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Kim MJ, Choe KM.
Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
PLoS Genet (2014) 10(10) e1004683
[
PubMed ID = 25329560
]
[
RRC reference
]
Xie X, Gilbert M, Petley-Ragan L, Auld VJ.
Loss of focal adhesions in glia disrupts both glial and photoreceptor axon migration in the Drosophila visual system.
Development (2014) 141(15) 3072-83
[
PubMed ID = 25053436
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Perkins AD, Ellis SJ, Asghari P, Shamsian A, Moore ED, Tanentzapf G.
Integrin-mediated adhesion maintains sarcomeric integrity.
Dev Biol (2010) 338(1) 15-27
[
PubMed ID = 19879257
]
[
RRC reference
]
Meghana C, Ramdas N, Hameed FM, Rao M, Shivashankar GV, Narasimha M.
Integrin adhesion drives the emergent polarization of active cytoskeletal stresses to pattern cell delamination.
Proc Natl Acad Sci U S A (2011) 108(22) 9107-12
[
PubMed ID = 21571643
]
[
RRC reference
]
Xie X, Auld VJ.
Integrins are necessary for the development and maintenance of the glial layers in the Drosophila peripheral nerve.
Development (2011) 138(17) 3813-22
[
PubMed ID = 21828098
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGATCCTCGAGAGAAACCGG |
| Primer Seq. 3' |
aatctagaggtaccGCAATGCGAGTCGCATGGACT |
| Predicted Fragment Size |
496 |
| IR fragment full Seq |
0001 ATGATCCTCG AGAGAAACCG GAGgtGCCAG CTGGcCCTCC TCATGATCGC AATACTGGCC
0061 GCCATCGCTG GACAAACGGA TGCCCAGAAG GCGGCCAAAC TGACGGCAGT NGAGCACCTG
0121 TGCATCGAAG GAAAAGTGTC ACACCTGCAT CCAGACAGAG GGTTGCGCCT GGTGCATGCA
0181 GCCGGACTTT AAGGGCCAGT CGCGGTGCTA CCAAAACACC AGCTCACTGT GTCCGGAGGA
0241 GTTCGCCTAC AGTCCGATAA CAGTTGAGCA GATTCTAGTG AACAACAAAC TAACGAATCA
0301 ATACAAAGCT GAACTGGCGG CTGGTGGCGG CGGCTCcGCC ATGTCCGGCA GCAGCTCAAG
0361 CTCCTACTCG TCGAGTTCGA GCTCGTCGAG CTTCTACTCG CAGAGCTCCT CGGGATCCAG
0421 TTCCGCCAGC GGATACGAAG AGTACTCTGC CGGCGAAATT GTCCAAATCC AACCGCAGTN
0481 CCATGCGANC TCGCATTGGG NACCTC |
| in silico PCR Fragment |
0001 ATGATCCTCG AGAGAAACCG GAGGTGCCAG CTGGCCCTCC TCATGATCGC AATACTGGCC
0061 GCCATCGCTG GACAAACGGA TGCCCAGAAG GCGGCCAAAC TGACGGCAGT -GAGCACATG
0121 TGCATCGAAG GAAAAGTGTC ACACCTGCAT CCAGACAGAG GGTTGCGCCT GGTGCATGCA
0181 GCCGGACTTT AAGGGCCAGT CGCGGTGCTA CCAAAACACC AGCTCACTGT GTCCGGAGGA
0241 GTTCGCCTAC AGTCCGATAA CAGTTGAGCA GATTCTAGTG AACAACAAAC TAACGAATCA
0301 ATACAAAGCT GAACTGGCGG CTGGTGGCGG CGGCTCCGCC ATGTCCGGCA GCAGCTCAAG
0361 CTCCTACTCG TCGAGTTCGA GCTCGTCGAG CTTCTACTCG CAGAGCTCCT CGGGATCCAG
0421 TTCCGCCAGC GGATACGAAG AGTACTCTGC CGGCGAAATT GTCCAAATCC AACCGCAGT-
0481 CCATGCGA-C TCGCATTG-- -----C |
| Assemble Data |
1560R-1.IR_full 1 ATGATCCTCGAGAGAAACCGGAGGTGCCAGCTGGCCCTCCTCATGATCGCAATACTGGCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1560R-1.in silico 1 ATGATCCTCGAGAGAAACCGGAGGTGCCAGCTGGCCCTCCTCATGATCGCAATACTGGCC 60
1560R-1.IR_full 61 GCCATCGCTGGACAAACGGATGCCCAGAAGGCGGCCAAACTGACGGCAGTNGAGCACCTG 120
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||
1560R-1.in silico 61 GCCATCGCTGGACAAACGGATGCCCAGAAGGCGGCCAAACTGACGGCAGT-GAGCACATG 120
1560R-1.IR_full 121 TGCATCGAAGGAAAAGTGTCACACCTGCATCCAGACAGAGGGTTGCGCCTGGTGCATGCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1560R-1.in silico 121 TGCATCGAAGGAAAAGTGTCACACCTGCATCCAGACAGAGGGTTGCGCCTGGTGCATGCA 180
1560R-1.IR_full 181 GCCGGACTTTAAGGGCCAGTCGCGGTGCTACCAAAACACCAGCTCACTGTGTCCGGAGGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1560R-1.in silico 181 GCCGGACTTTAAGGGCCAGTCGCGGTGCTACCAAAACACCAGCTCACTGTGTCCGGAGGA 240
1560R-1.IR_full 241 GTTCGCCTACAGTCCGATAACAGTTGAGCAGATTCTAGTGAACAACAAACTAACGAATCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1560R-1.in silico 241 GTTCGCCTACAGTCCGATAACAGTTGAGCAGATTCTAGTGAACAACAAACTAACGAATCA 300
1560R-1.IR_full 301 ATACAAAGCTGAACTGGCGGCTGGTGGCGGCGGCTCCGCCATGTCCGGCAGCAGCTCAAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1560R-1.in silico 301 ATACAAAGCTGAACTGGCGGCTGGTGGCGGCGGCTCCGCCATGTCCGGCAGCAGCTCAAG 360
1560R-1.IR_full 361 CTCCTACTCGTCGAGTTCGAGCTCGTCGAGCTTCTACTCGCAGAGCTCCTCGGGATCCAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1560R-1.in silico 361 CTCCTACTCGTCGAGTTCGAGCTCGTCGAGCTTCTACTCGCAGAGCTCCTCGGGATCCAG 420
1560R-1.IR_full 421 TTCCGCCAGCGGATACGAAGAGTACTCTGCCGGCGAAATTGTCCAAATCCAACCGCAGTN 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1560R-1.in silico 421 TTCCGCCAGCGGATACGAAGAGTACTCTGCCGGCGAAATTGTCCAAATCCAACCGCAGT- 480
1560R-1.IR_full 481 CCATGCGANCTCGCATTGGGNACCTC 506
|||||||| ||||||||| |
1560R-1.in silico 481 CCATGCGA-CTCGCATTG-------C 506
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
478 |
0 |
0 |
4 |
NM_080054.2 |
CG1560-RA (mys), mRNA |
| 0.62 |
3 |
3 |
2 |
2 |
NM_135281.1 |
CG6630-RA (CG6630), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_164749.1 |
CG5261-RA, transcript variant A (CG5261), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_135274.2 |
CG5261-RB, transcript variant B (CG5261), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_176480.1 |
CG33098-RB, transcript variant B (CG33098), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_176481.1 |
CG33098-RC, transcript variant C (CG33098), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_132655.1 |
CG1662-RA (CG1662), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_166450.1 |
CG10497-RC, transcript variant C (Sdc), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_166449.2 |
CG10497-RB, transcript variant B (Sdc), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_057617.3 |
CG10497-RA, transcript variant A (Sdc), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_176482.1 |
CG33098-RD, transcript variant D (CG33098), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_142769.2 |
CG5383-RA (PSR), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_168477.1 |
CG32092-RB (CG32092), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_136733.2 |
CG12907-RA (CG12907), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001043113.1 |
CG33484-RC, transcript variant C (zormin), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168802.1 |
CG32210-RA (CG32210), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057543.3 |
CG9998-RA (U2af50), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001014584.1 |
CG4879-RC, transcript variant C (RecQ5), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_079346.2 |
CG4879-RA, transcript variant A (RecQ5), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_139528.2 |
CG17569-RB, transcript variant B (gry), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_168000.1 |
CG17569-RA, transcript variant A (gry), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_143316.2 |
CG5586-RB (Tusp), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140050.2 |
CG4022-RA (CG4022), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143303.1 |
CG6599-RA (CG6599), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167469.2 |
CG10545-RB, transcript variant B (Gbeta13F), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080351.5 |
CG10545-RA, transcript variant A (Gbeta13F), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206737.1 |
CG10545-RE, transcript variant E (Gbeta13F), mRNA |
|
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