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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15433R-3 |
| Symbol |
Elp3 |
Full Name |
Elp3 |
| CG No |
CG15433 |
Old CG No |
CG15433 |
| Synonyms |
dmHAG408, Dmel/ELP3, HAT, Elp3, Dmel?ELP3, CG15433 |
| Accession No (Link to NCBI) |
NM_134990.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Planelles-Herrero VJ, Bittleston A, Seum C, Gaitan MG, Derivery E.<br> Elongator stabilizes microtubules to control central spindle asymmetry and polarized trafficking of cell fate determinants.<br> Nat Cell Biol (2022) 24(11) 1606-1616
[
PubMed ID = 36302967
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Walker J, Kwon SY, Badenhorst P, East P, McNeill H, Svejstrup JQ.<br> Role of elongator subunit Elp3 in Drosophila melanogaster larval development and immunity.<br> Genetics (2011) 187(4) 1067-75
[
PubMed ID = 21288872
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCTTCAGACCCGACACCGCGT |
| Primer Seq. 3' |
aatctagaggtaccAGCGGAAGGCAGGGTTCTCAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCTTCAGACC CGACACCGCG TTGAACAGCT GAAGCAGCTG GGACACAGTG TGGACAAGGT
0061 GGAGTTCATC GTAATGGGCG GTACGTTCAT GTGCCTGCCG GAGGAGTACC GCGACTACTT
0121 TATCCGCAAC CTACACNGAC GCCCTCTCCG GCCACAGCAG CGCCAATGTG GCGGAAGCCG
0181 TANCGCTACT CGGAGAAGTC ACGCACAAAG TGCATTGGCA TCACCATAGA AACAAGGCCA
0241 GATTACTGCC TAAAGCGGCA CATATCGGAT ATGCTTAGCT ATGGTTGCAC CCGGCTGGAA
0301 ATCGGTGTGC AATCGGTTTA CGANAGACGT GGCCAGGGAT ACGAACCGCG GACACACGGT
0361 GCGAGCGGTG TGCGAGAGCT TCCAGCTGGG CAAGGATGCT GGCTACAAGA TCGTGACGCA
0421 CATGATGCCT GATCTTCCCA ATGTTGACTT TGAACGCGAC ATCGAGCAGT TTATCGAGTA
0481 CTTTGAGAAC CCTGCCTTCC GCT |
| in silico PCR Fragment |
0001 TCTTCAGACC CGACACCGCG TTGAACAGCT GAAGCAGCTG GGACACAGTG TGGACAAGGT
0061 GGAGTTCATC GTAATGGGCG GTACGTTCAT GTGCCTGCCG GAGGAGTACC GCGACTACTT
0121 TATCCGCAAC CTACAC-GAC GCCCTCTCCG GCCACAGCAG CGCCAATGTG GCGGAAGCCG
0181 TA-CGCTACT CGGAGAAGTC ACGCACAAAG TGCATTGGCA TCACCATAGA AACAAGGCCA
0241 GATTACTGCC TAAAGCGGCA CATATCGGAT ATGCTTAGCT ATGGTTGCAC CCGGCTGGAA
0301 ATCGGTGTGC AATCGGTTTA CGA-AGACGT GGCCAGGGAT ACGAACCGCG GACACACGGT
0361 GCGAGCGGTG TGCGAGAGCT TCCAGCTGGG CAAGGATGCT GGCTATAAGA TCGTGACGCA
0421 CATGATGCCT GATCTTCCCA ATGTTGACTT TGAACGCGAC ATCGAGCAGT TTATCGAGTA
0481 CTTTGAGAAC CCTGCCTTCC GCT |
| Assemble Data |
<PRE>
15433R-3.IR_full 1 TCTTCAGACCCGACACCGCGTTGAACAGCTGAAGCAGCTGGGACACAGTGTGGACAAGGT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15433R-3.in silico 1 TCTTCAGACCCGACACCGCGTTGAACAGCTGAAGCAGCTGGGACACAGTGTGGACAAGGT 60
15433R-3.IR_full 61 GGAGTTCATCGTAATGGGCGGTACGTTCATGTGCCTGCCGGAGGAGTACCGCGACTACTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15433R-3.in silico 61 GGAGTTCATCGTAATGGGCGGTACGTTCATGTGCCTGCCGGAGGAGTACCGCGACTACTT 120
15433R-3.IR_full 121 TATCCGCAACCTACAC<span class="snp"><tt>N</tt></span>GACGCCCTCTCCGGCCACAGCAGCGCCAATGTGGCGGAAGCCG 180
||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||
15433R-3.in silico 121 TATCCGCAACCTACAC<span class="snp"><tt>-</tt></span>GACGCCCTCTCCGGCCACAGCAGCGCCAATGTGGCGGAAGCCG 180
15433R-3.IR_full 181 TA<span class="snp"><tt>N</tt></span>CGCTACTCGGAGAAGTCACGCACAAAGTGCATTGGCATCACCATAGAAACAAGGCCA 240
||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15433R-3.in silico 181 TA<span class="snp"><tt>-</tt></span>CGCTACTCGGAGAAGTCACGCACAAAGTGCATTGGCATCACCATAGAAACAAGGCCA 240
15433R-3.IR_full 241 GATTACTGCCTAAAGCGGCACATATCGGATATGCTTAGCTATGGTTGCACCCGGCTGGAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15433R-3.in silico 241 GATTACTGCCTAAAGCGGCACATATCGGATATGCTTAGCTATGGTTGCACCCGGCTGGAA 300
15433R-3.IR_full 301 ATCGGTGTGCAATCGGTTTACGA<span class="snp"><tt>N</tt></span>AGACGTGGCCAGGGATACGAACCGCGGACACACGGT 360
|||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||
15433R-3.in silico 301 ATCGGTGTGCAATCGGTTTACGA<span class="snp"><tt>-</tt></span>AGACGTGGCCAGGGATACGAACCGCGGACACACGGT 360
15433R-3.IR_full 361 GCGAGCGGTGTGCGAGAGCTTCCAGCTGGGCAAGGATGCTGGCTA<span class="snp"><tt>C</tt></span>AAGATCGTGACGCA 420
|||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||
15433R-3.in silico 361 GCGAGCGGTGTGCGAGAGCTTCCAGCTGGGCAAGGATGCTGGCTA<span class="snp"><tt>T</tt></span>AAGATCGTGACGCA 420
15433R-3.IR_full 421 CATGATGCCTGATCTTCCCAATGTTGACTTTGAACGCGACATCGAGCAGTTTATCGAGTA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15433R-3.in silico 421 CATGATGCCTGATCTTCCCAATGTTGACTTTGAACGCGACATCGAGCAGTTTATCGAGTA 480
15433R-3.IR_full 481 CTTTGAGAACCCTGCCTTCCGCT 503
|||||||||||||||||||||||
15433R-3.in silico 481 CTTTGAGAACCCTGCCTTCCGCT 503
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_134990.2 |
CG15433-RA (CG15433), mRNA |
| 0 |
0 |
6 |
2 |
5 |
NM_136192.2 |
CG2508-RA (cdc23), mRNA |
| 0 |
0 |
6 |
2 |
5 |
NM_165331.2 |
CG31687-RA (CG31687), mRNA |
| 0 |
0 |
5 |
4 |
4 |
NM_134495.2 |
CG12234-RA (Ranbp21), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_169972.1 |
CG31336-RA (Gr93b), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169870.1 |
CG6184-RB, transcript variant B (CG6184), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_142567.1 |
CG6184-RA, transcript variant A (CG6184), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169871.1 |
CG6184-RC, transcript variant C (CG6184), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_134927.1 |
CG15412-RA (CG15412), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_134504.2 |
CG14232-RA (CG14232), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_057214.3 |
CG4807-RA, transcript variant A (ab), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_057215.3 |
CG4807-RB, transcript variant B (ab), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_176458.2 |
CG4509-RB (CG4509), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137944.2 |
CG9891-RA (yellow-d2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141932.2 |
CG11466-RA, transcript variant A (Cyp9f2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_206478.1 |
CG11466-RB, transcript variant B (Cyp9f2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079647.2 |
CG8884-RF, transcript variant F (Sap47), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169690.1 |
CG8884-RB, transcript variant B (Sap47), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169685.1 |
CG8884-RC, transcript variant C (Sap47), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169689.1 |
CG8884-RG, transcript variant G (Sap47), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169687.1 |
CG8884-RE, transcript variant E (Sap47), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169686.1 |
CG8884-RD, transcript variant D (Sap47), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169684.1 |
CG8884-RA, transcript variant A (Sap47), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169688.1 |
CG8884-RH, transcript variant H (Sap47), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166934.1 |
CG3191-RA (CG3191), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143153.2 |
CG4719-RA (tankyrase), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057632.3 |
CG10072-RA (sgl), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136774.1 |
CG12325-RA (CG12325), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_138984.2 |
CG5712-RA (ACXD), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_168126.1 |
CG5406-RB, transcript variant B (sif), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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