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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1539R-1 |
| Symbol |
tmod |
Full Name |
tropomodulin |
| CG No |
CG1539 |
Old CG No |
CG1539 |
| Synonyms |
l(3)S130910, CG1539, spdo, Tmod, l(3)00848, E42, 1309/10, 0970/02, unnamed, CG15540, CG11493, tmod, tropomodulin, Tropomodulin |
| Accession No (Link to NCBI) |
NM_057683.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Molnár I, Migh E, Szikora S, Kalmár T, Végh AG, Deák F, Barkó S, Bugyi B, Orfanos Z, Kovács J, Juhász G, Váró G, Nyitrai M, Sparrow J, Mihály J.<br> DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila.<br> PLoS Genet (2014) 10(2) e1004166
[
PubMed ID = 24586196
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTGTATGGCAAGGATCTGAGT |
| Primer Seq. 3' |
aatctagaggtaccTTCGTAATGCCATCCCATCCC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTGTATGGCA AGGATCTGAG TGAGTACGAT GATGTGGATG TGGAGAGCCT GCTGGCCCAG
0061 CTCTCCCCCG AGGAGATAAC CATACTGGCC AAAGAAGTGG ATCCCGATGA CAACTTCCTG
0121 CCGCCAGACC AGCGCAATAG CCTATGAATG CACCAAGGAA GCCACCGGGC CTCTCAATCG
0181 CAAGCAGCTG ATCGAGCACA TCAATAAGCA GGCCATCGAG ACCCCGGATC AGCCGGAGTT
0241 CGAGCCGTTC GTTCAGGGCA AAGTTCGTGG AAAGAAGTGG GTCCCACCGC CAAGGGATGC
0301 CCGCGACATC GAGGCCGAAG AACAAATCGC CATTGATATG GGCGAGGAGT ACGAACACGC
0361 CCTTAACGAT GCCACGCAGG AGGAGATCAT CGATCTGGCC GCCATCCTCG GCTTCCACTC
0421 GATGATGAAT CAGGACCAGT ACCACGCCTC CTTGCTCAAC AAGGGTCAgN CCGTGGGCCT
0481 GGGATGGGAT GGCATTACGA A |
| in silico PCR Fragment |
0001 CTGTATGGCA AGGATCTGAG TGAGTACGAT GATGTGGATG TGGAGAGCCT GCTGGCCCAG
0061 CTCTCCCCCG AGGAGATAAC CATACTGGCC AAAGAAGTGG ATCCCGATGA CAACTTCCTG
0121 CCGCCAGACC AGCGCAATAG -CTATGAGTG CACCAAGGAA GCCACCGGGC CTCTCAATCG
0181 CAAGCAGCTG ATCGAGCACA TCAATAAGCA GGCCATCGAG ACCCCGGATC AGCCGGAGTT
0241 CGAGCCGTTC GTTCAGGGCA AGGTTCGTGG AAAGAAGTGG GTCCCACCGC CAAGGGATGC
0301 CCGCGACATC GAGGCCGAAG AACAAATCGC CATTGACATG GGCGAGGAGT ACGAACACGC
0361 CCTTAACGAT GCCACGCAGG AGGAGATCAT CGATCTGGCC GCCATCCTCG GCTTCCACTC
0421 GATGATGAAT CAGGACCAGT ACCACGCCTC CTTGCTCAAC AAGGGTCAGC CCGTGGGCCT
0481 GGGATGGGAT GGCATTACGA A |
| Assemble Data |
<PRE>
1539R-1.IR_full 1 CTGTATGGCAAGGATCTGAGTGAGTACGATGATGTGGATGTGGAGAGCCTGCTGGCCCAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1539R-1.in silico 1 CTGTATGGCAAGGATCTGAGTGAGTACGATGATGTGGATGTGGAGAGCCTGCTGGCCCAG 60
1539R-1.IR_full 61 CTCTCCCCCGAGGAGATAACCATACTGGCCAAAGAAGTGGATCCCGATGACAACTTCCTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1539R-1.in silico 61 CTCTCCCCCGAGGAGATAACCATACTGGCCAAAGAAGTGGATCCCGATGACAACTTCCTG 120
1539R-1.IR_full 121 CCGCCAGACCAGCGCAATAG<span class="snp"><tt>C</tt></span>CTATGA<span class="snp"><tt>A</tt></span>TGCACCAAGGAAGCCACCGGGCCTCTCAATCG 180
||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||
1539R-1.in silico 121 CCGCCAGACCAGCGCAATAG<span class="snp"><tt>-</tt></span>CTATGA<span class="snp"><tt>G</tt></span>TGCACCAAGGAAGCCACCGGGCCTCTCAATCG 180
1539R-1.IR_full 181 CAAGCAGCTGATCGAGCACATCAATAAGCAGGCCATCGAGACCCCGGATCAGCCGGAGTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1539R-1.in silico 181 CAAGCAGCTGATCGAGCACATCAATAAGCAGGCCATCGAGACCCCGGATCAGCCGGAGTT 240
1539R-1.IR_full 241 CGAGCCGTTCGTTCAGGGCAA<span class="snp"><tt>A</tt></span>GTTCGTGGAAAGAAGTGGGTCCCACCGCCAAGGGATGC 300
|||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||
1539R-1.in silico 241 CGAGCCGTTCGTTCAGGGCAA<span class="snp"><tt>G</tt></span>GTTCGTGGAAAGAAGTGGGTCCCACCGCCAAGGGATGC 300
1539R-1.IR_full 301 CCGCGACATCGAGGCCGAAGAACAAATCGCCATTGA<span class="snp"><tt>T</tt></span>ATGGGCGAGGAGTACGAACACGC 360
||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
1539R-1.in silico 301 CCGCGACATCGAGGCCGAAGAACAAATCGCCATTGA<span class="snp"><tt>C</tt></span>ATGGGCGAGGAGTACGAACACGC 360
1539R-1.IR_full 361 CCTTAACGATGCCACGCAGGAGGAGATCATCGATCTGGCCGCCATCCTCGGCTTCCACTC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1539R-1.in silico 361 CCTTAACGATGCCACGCAGGAGGAGATCATCGATCTGGCCGCCATCCTCGGCTTCCACTC 420
1539R-1.IR_full 421 GATGATGAATCAGGACCAGTACCACGCCTCCTTGCTCAACAAGGGTCAG<span class="snp"><tt>N</tt></span>CCGTGGGCCT 480
|||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||
1539R-1.in silico 421 GATGATGAATCAGGACCAGTACCACGCCTCCTTGCTCAACAAGGGTCAG<span class="snp"><tt>C</tt></span>CCGTGGGCCT 480
1539R-1.IR_full 481 GGGATGGGATGGCATTACGAA 501
|||||||||||||||||||||
1539R-1.in silico 481 GGGATGGGATGGCATTACGAA 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_170504.1 |
CG1539-RE, transcript variant E (tmod), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_170503.1 |
CG1539-RD, transcript variant D (tmod), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_170505.1 |
CG1539-RF, transcript variant F (tmod), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_170506.1 |
CG1539-RC, transcript variant C (tmod), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_057683.2 |
CG1539-RA, transcript variant A (tmod), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_057684.2 |
CG1539-RB, transcript variant B (tmod), mRNA |
| 0.2 |
1 |
3 |
4 |
2 |
NM_139966.2 |
CG18543-RA (mtrm), mRNA |
| 0 |
0 |
3 |
4 |
7 |
NM_132159.1 |
CG1677-RA (CG1677), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_139778.2 |
CG10274-RA (CG10274), mRNA |
| 0 |
0 |
2 |
6 |
7 |
NM_139588.3 |
CG12605-RB, transcript variant B (CG12605), mRNA |
| 0 |
0 |
2 |
6 |
7 |
NM_206275.2 |
CG12605-RA, transcript variant A (CG12605), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_001014543.1 |
CG6741-RC, transcript variant C (a), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_166514.1 |
CG6741-RA, transcript variant A (a), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_079902.2 |
CG6741-RB, transcript variant B (a), mRNA |
| 0 |
0 |
2 |
3 |
14 |
NM_168898.1 |
CG32436-RA (CG32436), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132483.2 |
CG1737-RA (CG1737), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_079258.2 |
CG5939-RA, transcript variant A (Prm), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_168290.1 |
CG5939-RB, transcript variant B (Prm), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_206430.1 |
CG2666-RC, transcript variant C (kkv), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_169052.1 |
CG2666-RB, transcript variant B (kkv), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_079509.1 |
CG2666-RA, transcript variant A (kkv), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_132956.1 |
CG9086-RA (CG9086), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_132759.1 |
CG9503-RA (CG9503), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_135176.2 |
CG9506-RA (slam), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168291.1 |
CG5939-RC, transcript variant C (Prm), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168292.1 |
CG5939-RD, transcript variant D (Prm), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_136050.2 |
CG31751-RB, transcript variant B (CG31751), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_165260.1 |
CG31751-RA, transcript variant A (CG31751), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057732.3 |
CG8203-RA (Cdk5), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_168879.2 |
CG9936-RC, transcript variant C (skd), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04820
:
Cytoskeleton in muscle cells
|
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