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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

15010R-2

Symbol

ago

Full Name

archipelago

CG No

CG15010

Old CG No

CG15010

Synonyms

cdc4, SCF[Ago], CG15010, DmFbw7, anon-WO0118547.345, ago, Ago

Accession No (Link to NCBI)

NM_168072.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

semi-lethal

Map Viewer

Reference
[Please submit your publication]

Zahoor MK, Poidevin M, Lecerf C, Garrido D, Montagne J.
A Drosophila genetic screen for suppressors of S6kinase-dependent growth identifies the F-box subunit Archipelago/FBXW7.
Mol Genet Genomics (2019) 294(3) 573-582 [ PubMed ID = 30656413 ] [ RRC reference ]

Chen ZS, Wong AKY, Cheng TC, Koon AC, Chan HYE.
FipoQ/FBXO33, a Cullin-1-based ubiquitin ligase complex component modulates ubiquitination and solubility of polyglutamine disease protein.
J Neurochem (2019) 149(6) 781-798 [ PubMed ID = 30685895 ] [ RRC reference ]

Eusebio N, Tavares L, Pereira PS.
CtBP represses Dpp-dependent Mad activation during Drosophila eye development.
Dev Biol (2018) 442(1) 188-198 [ PubMed ID = 30031756 ] [ RRC reference ]

Dui W, Lu W, Ma J, Jiao R.
A systematic phenotypic screen of F-box genes through a tissue-specific RNAi-based approach in Drosophila.
J Genet Genomics (2012) 39(8) 397-413 [ PubMed ID = 22884096 ] [ RRC reference ]

Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168 [ PubMed ID = 21931660 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCCAGCTGCTAGCAGTGAATC

Primer Seq. 3'

aatctagaggtaccCCTCATCATCGTCATCGTCGT

Predicted Fragment Size

IR fragment full Seq

0001 CCCAGCTgCT AGCAGTGAAT CCGTGACCAG TGCAGGGGAG CGTACTCAGT CAGCAGTCAC 
0061 CAGCTCCACC TCCACGTGGG TCAAGAGTCA GGCCTCAACC AGCCGAAAAA CAGAAGCATC 
0121 AGAAGAATCG GGATTAGGAG CAGTAGACGC AGAAGTAGGA GCCGGCAGAG AAGCCTTTGT 
0181 ATCTATGTCA ACGCTGCGGG AGGATGTGGA GGACGTGTGT GTGTCGTCAA ACTCACAACA 
0241 TGGTTTCGCC GTAGTTTTGG ACGACGAGTC CAGCACATTT GAGATTAGCT CTTCCAACTC 
0301 ACTGCCCACC AGTGCGGGGG CGGCGTCCAC AGTGGGAGTG GTGGCCGTCG ACGACAGCTC 
0361 CTCCACGGAT ACCTTGAACG GTGGTCATCC TGACTTAGGT CACCCCGCCT CATCGGAACA 
0421 CAGTCGTCAG GGCTTTTTTA ATGAGGATAA TGAAGATcCT CCGGTTGTAT GCCTTATAAA 
0481 CGACGATGAC GATGATGAGG

in silico PCR Fragment

0001 CCCAGCTGCT AGCAGTGAAT CCGTGACCAG TGCAGGGGAG CGTACTCAGT CAGCAGTCAC 
0061 CAGCTCCACC TCCACGTGGG TCAAGAGTCA GGCCTCAACC AGCCGAAAAA CAGAAGCATC 
0121 AGAAGAATCG GGATTAGGAG CAGTAGACGC AGAAGTAGGA GCCGGCAGAG AAGCCTTTGT 
0181 ATCTATGTCA ACGCTGCGGG AGGATGTGGA GGACGTGTGT GTGTCGTCAA ACTCACAACA 
0241 TGGTTTCGCC GTAGTTTTGG ACGACGAGTC CAGCACATTT GAGATTAGCT CTTCCAACTC 
0301 ACTGCCCACC AGTGCGGGGG CGGCGTCCAC AGTGGGAGTG GTGGCCGTCG ACGACAGCTC 
0361 CTCCACGGAT ACCTTGAACG GTGGTCATCC TGACTTAGGT CACCCCGCCT CATCGGAACA 
0421 CAGTCGTCAG GGCTTTTTTA ATGAGGATAA TGAAGATCCT CCGGTTGTAT GCCTTATAAA 
0481 CGACGATGAC GATGATGAGG

Assemble Data


15010R-2.IR_full       1   CCCAGCTGCTAGCAGTGAATCCGTGACCAGTGCAGGGGAGCGTACTCAGTCAGCAGTCAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15010R-2.in silico     1   CCCAGCTGCTAGCAGTGAATCCGTGACCAGTGCAGGGGAGCGTACTCAGTCAGCAGTCAC 60


15010R-2.IR_full       61  CAGCTCCACCTCCACGTGGGTCAAGAGTCAGGCCTCAACCAGCCGAAAAACAGAAGCATC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15010R-2.in silico     61  CAGCTCCACCTCCACGTGGGTCAAGAGTCAGGCCTCAACCAGCCGAAAAACAGAAGCATC 120


15010R-2.IR_full       121 AGAAGAATCGGGATTAGGAGCAGTAGACGCAGAAGTAGGAGCCGGCAGAGAAGCCTTTGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15010R-2.in silico     121 AGAAGAATCGGGATTAGGAGCAGTAGACGCAGAAGTAGGAGCCGGCAGAGAAGCCTTTGT 180


15010R-2.IR_full       181 ATCTATGTCAACGCTGCGGGAGGATGTGGAGGACGTGTGTGTGTCGTCAAACTCACAACA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15010R-2.in silico     181 ATCTATGTCAACGCTGCGGGAGGATGTGGAGGACGTGTGTGTGTCGTCAAACTCACAACA 240


15010R-2.IR_full       241 TGGTTTCGCCGTAGTTTTGGACGACGAGTCCAGCACATTTGAGATTAGCTCTTCCAACTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15010R-2.in silico     241 TGGTTTCGCCGTAGTTTTGGACGACGAGTCCAGCACATTTGAGATTAGCTCTTCCAACTC 300


15010R-2.IR_full       301 ACTGCCCACCAGTGCGGGGGCGGCGTCCACAGTGGGAGTGGTGGCCGTCGACGACAGCTC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15010R-2.in silico     301 ACTGCCCACCAGTGCGGGGGCGGCGTCCACAGTGGGAGTGGTGGCCGTCGACGACAGCTC 360


15010R-2.IR_full       361 CTCCACGGATACCTTGAACGGTGGTCATCCTGACTTAGGTCACCCCGCCTCATCGGAACA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15010R-2.in silico     361 CTCCACGGATACCTTGAACGGTGGTCATCCTGACTTAGGTCACCCCGCCTCATCGGAACA 420


15010R-2.IR_full       421 CAGTCGTCAGGGCTTTTTTAATGAGGATAATGAAGATCCTCCGGTTGTATGCCTTATAAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15010R-2.in silico     421 CAGTCGTCAGGGCTTTTTTAATGAGGATAATGAAGATCCTCCGGTTGTATGCCTTATAAA 480


15010R-2.IR_full       481 CGACGATGACGATGATGAGG 500
                           ||||||||||||||||||||
15010R-2.in silico     481 CGACGATGACGATGATGAGG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_079198.2	CG15010-RC, transcript variant C (ago), mRNA
100	482	0	0	1	NM_168073.1	CG15010-RB, transcript variant B (ago), mRNA
100	482	0	0	1	NM_168072.1	CG15010-RA, transcript variant A (ago), mRNA
0	0	4	6	2	NM_080189.2	CG16880-RA (l(2)34Fa), mRNA
0	0	4	4	19	NM_170139.1	CG31132-RA (BRWD3), mRNA
0	0	4	3	4	NM_168771.1	CG4144-RA, transcript variant A (GNBP2), mRNA
0	0	4	3	4	NM_168772.1	CG4144-RD, transcript variant D (GNBP2), mRNA
0	0	4	3	4	NM_079417.2	CG4144-RB, transcript variant B (GNBP2), mRNA
0	0	4	3	4	NM_168773.1	CG4144-RC, transcript variant C (GNBP2), mRNA
0	0	3	11	31	NM_140110.2	CG8108-RB, transcript variant B (CG8108), mRNA
0	0	3	11	31	NM_168386.1	CG8108-RA, transcript variant A (CG8108), mRNA
0	0	3	4	12	NM_130725.2	CG15239-RA (CG15239), mRNA
0	0	3	3	3	NM_170640.2	CG32464-RK, transcript variant K (l(3)82Fd), mRNA
0	0	3	3	3	NM_206429.1	CG32464-RG, transcript variant G (l(3)82Fd), mRNA
0	0	3	3	3	NM_169038.1	CG32464-RJ, transcript variant J (l(3)82Fd), mRNA
0	0	3	3	3	NM_001031977.1	CG32464-RN, transcript variant N (l(3)82Fd), mRNA
0	0	3	3	3	NM_001043213.1	CG32464-RP, transcript variant P (l(3)82Fd), mRNA
0	0	3	3	3	NM_143760.2	CG32464-RB, transcript variant B (l(3)82Fd), mRNA
0	0	3	3	3	NM_169039.2	CG32464-RL, transcript variant L (l(3)82Fd), mRNA
0	0	3	3	3	NM_001043214.1	CG32464-RO, transcript variant O (l(3)82Fd), mRNA
0	0	3	3	2	NM_001031978.1	CG32464-RM, transcript variant M (l(3)82Fd), mRNA
0	0	3	3	2	NM_170641.1	CG32464-RF, transcript variant F (l(3)82Fd), mRNA
0	0	3	3	2	NM_170644.1	CG32464-RA, transcript variant A (l(3)82Fd), mRNA
0	0	3	3	2	NM_169045.1	CG32464-RH, transcript variant H (l(3)82Fd), mRNA
0	0	3	3	2	NM_170643.1	CG32464-RC, transcript variant C (l(3)82Fd), mRNA
0	0	3	3	2	NM_170642.1	CG32464-RI, transcript variant I (l(3)82Fd), mRNA
0	0	3	2	9	NM_167218.1	CG32685-RC (CG32685), mRNA
0	0	3	2	6	NM_142706.2	CG3308-RA (CG3308), mRNA
0	0	3	2	6	NM_142705.1	CG5919-RA (CG5919), mRNA
0	0	2	8	7	NM_164957.1	CG6181-RB, transcript variant B (CG6181), mRNA

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