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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15006R-2

Symbol Cpr64Aa Full Name Cuticular protein 64Aa

CG No CG15006 Old CG No CG15006

Synonyms Cpr64Aa, CG15006

Accession No (Link to NCBI) NM_139615.2

Inserted Chr. X

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965 [ PubMed ID = 25647511 ] [ RRC reference ]

Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zoolog Sci (2016) 33(6) 583-591 [ PubMed ID = 27927092 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAACTGATCCTCGTCCTGAGCG

Primer Seq. 3'

aatctagaggtaccTTGGCCACCAGAGGAGAGGCA

Predicted Fragment Size

IR fragment full Seq

0001 AACTGATCCT CGTCCTGAGC GCCCTGGTGG cGGTGTCCTC CGCCGTTGTG GTCCCTGGTC 
0061 CCGGACTGGC ACTGCCCGCC TATCCATCGT ACCCGGCGCT GGCCAAGGTG GCTGCTCCTC 
0121 TGGTGGCCAA GGTGGCGGGT CCGGAGCCAT ACGATCCCAA TCCCCAATAC ACCTTCAGCT 
0181 ACGATGTTCA TGATGGCTCC ACTGGTGACG TGAAGAGCCA GCAGGAGACC CGCAGCGGAG 
0241 ATGTGGTGCA GGGCGCCTNA TTCACTGATT GAGGCCGACG GAACCCGCCG CATTGTGGAG 
0301 TACACCGCCG ATCCCGTGCA CGGATTCAAC GCAGTGGTGC GCCGCGAGGG AGCTGTGGTG 
0361 AAGGCTGTGG CTCCGGTGGC CAAGGTTCTG GCCCCGCCTC CGCTGCTCCA TGCCTCTCCT 
0421 CTGGTGGCCA A

in silico PCR Fragment

0001 AACTGATCCT CGTCCTGAGC GCCCTGGTGG CGGTGTCCTC CGCCGTTGTG GTCCCTGGTC 
0061 CCGGACTGGC ACTGCCCGCC TATCCATCGT ACCCGGCGCT GGCCAAGGTG GCTGCTCCTC 
0121 TGGTGGCCAA GGTGGCGGGT CCGGAGCCAT ACGATCCCAA TCCCCAATAC ACCTTCAGCT 
0181 ACGATGTTCA TGATGGCTCC ACTGGTGACG TGAAGAGCCA GCAGGAGACC CGCAGCGGAG 
0241 ATGTGGTGCA GGGCGCCT-A TTCACTGATT GAGGCCGACG GAACCCGCCG CATTGTGGAG 
0301 TACACCGCCG ATCCCGTGCA CGGATTCAAC GCAGTGGTGC GCCGCGAGGG AGCTGTGGTG 
0361 AAGGCTGTGG CTCCGGTGGC CAAGGTTCTG GCCCCGGCTC CGCTGCTCCA TGCCTCTCCT 
0421 CTGGTGGCCA A

Assemble Data


15006R-2.IR_full       1   AACTGATCCTCGTCCTGAGCGCCCTGGTGGCGGTGTCCTCCGCCGTTGTGGTCCCTGGTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15006R-2.in silico     1   AACTGATCCTCGTCCTGAGCGCCCTGGTGGCGGTGTCCTCCGCCGTTGTGGTCCCTGGTC 60


15006R-2.IR_full       61  CCGGACTGGCACTGCCCGCCTATCCATCGTACCCGGCGCTGGCCAAGGTGGCTGCTCCTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15006R-2.in silico     61  CCGGACTGGCACTGCCCGCCTATCCATCGTACCCGGCGCTGGCCAAGGTGGCTGCTCCTC 120


15006R-2.IR_full       121 TGGTGGCCAAGGTGGCGGGTCCGGAGCCATACGATCCCAATCCCCAATACACCTTCAGCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15006R-2.in silico     121 TGGTGGCCAAGGTGGCGGGTCCGGAGCCATACGATCCCAATCCCCAATACACCTTCAGCT 180


15006R-2.IR_full       181 ACGATGTTCATGATGGCTCCACTGGTGACGTGAAGAGCCAGCAGGAGACCCGCAGCGGAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15006R-2.in silico     181 ACGATGTTCATGATGGCTCCACTGGTGACGTGAAGAGCCAGCAGGAGACCCGCAGCGGAG 240


15006R-2.IR_full       241 ATGTGGTGCAGGGCGCCTNATTCACTGATTGAGGCCGACGGAACCCGCCGCATTGTGGAG 300
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
15006R-2.in silico     241 ATGTGGTGCAGGGCGCCT-ATTCACTGATTGAGGCCGACGGAACCCGCCGCATTGTGGAG 300


15006R-2.IR_full       301 TACACCGCCGATCCCGTGCACGGATTCAACGCAGTGGTGCGCCGCGAGGGAGCTGTGGTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15006R-2.in silico     301 TACACCGCCGATCCCGTGCACGGATTCAACGCAGTGGTGCGCCGCGAGGGAGCTGTGGTG 360


15006R-2.IR_full       361 AAGGCTGTGGCTCCGGTGGCCAAGGTTCTGGCCCCGCCTCCGCTGCTCCATGCCTCTCCT 420
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
15006R-2.in silico     361 AAGGCTGTGGCTCCGGTGGCCAAGGTTCTGGCCCCGGCTCCGCTGCTCCATGCCTCTCCT 420


15006R-2.IR_full       421 CTGGTGGCCAA 431
                           |||||||||||
15006R-2.in silico     421 CTGGTGGCCAA 431

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100.48	414	3	9	14	NM_139615.2	CG15006-RA (CG15006), mRNA
1.69	7	14	10	38	NM_141420.2	CG2342-RA (Ccp84Ag), mRNA
0	0	11	5	6	NM_057558.3	CG16963-RA (Cry), mRNA
0	0	8	6	5	NM_164877.1	CG31878-RB, transcript variant B (CG31878), mRNA
0	0	8	6	5	NM_164878.1	CG31878-RA, transcript variant A (CG31878), mRNA
0	0	3	4	12	NM_140864.1	CG9290-RA (CG9290), mRNA
0	0	3	4	9	NM_139618.2	CG1259-RB (CG1259), mRNA
0	0	3	3	3	NM_167522.2	CG4429-RB, transcript variant B (Rbp2), mRNA
0	0	3	3	3	NM_080220.2	CG4429-RA, transcript variant A (Rbp2), mRNA
0	0	3	3	3	NM_206764.1	CG4429-RC, transcript variant C (Rbp2), mRNA
0	0	3	2	5	NM_206398.1	CG33255-RA (CG33255), mRNA
0	0	2	11	18	NM_139617.1	CG15008-RA (CG15008), mRNA
0	0	2	10	6	NM_168797.1	CG32213-RA (CG32213), mRNA
0	0	2	10	6	NM_168796.1	CG32208-RA (825-Oak), mRNA
0	0	2	7	2	NM_139411.1	CG1919-RA (CG1919), mRNA
0	0	2	4	7	NM_079523.1	CG2345-RA (Edg84A), mRNA
0	0	2	3	8	NM_165103.2	CG3506-RA (vas), mRNA
0	0	2	3	6	NM_078768.2	CG9554-RB, transcript variant B (eya), mRNA
0	0	2	3	6	NM_164693.1	CG9554-RA, transcript variant A (eya), mRNA
0	0	2	3	4	NM_130571.1	CG14796-RA (CG14796), mRNA
0	0	2	2	3	NM_164429.1	CG31666-RC, transcript variant C (CG31666), mRNA
0	0	2	2	3	NM_164428.2	CG31666-RB, transcript variant B (CG31666), mRNA
0	0	2	2	3	NM_134766.3	CG31666-RA, transcript variant A (CG31666), mRNA
0	0	2	2	3	NM_164430.2	CG31666-RD, transcript variant D (CG31666), mRNA
0	0	1	12	7	NM_140857.2	CG12519-RA (CG12519), mRNA
0	0	1	10	35	NM_141423.2	CG2341-RA (Ccp84Ad), mRNA
0	0	1	9	38	NM_141426.1	CG2360-RA (Ccp84Aa), mRNA
0	0	1	9	32	NM_141425.1	CG1252-RA (Ccp84Ab), mRNA
0	0	1	5	4	NM_168795.1	CG32214-RA (CG32214), mRNA
0	0	1	4	27	NM_142556.2	CG6240-RA (CG6240), mRNA

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