|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 4 May to 11 May, deadline 21 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1462R-2 |
| Symbol |
Aph-4 |
Full Name |
Alkaline phosphatase 4 |
| CG No |
CG1462 |
Old CG No |
CG1462 |
| Synonyms |
CG1462, pMY51, l(3)07028, AP, unnamed, Aph, Aph-4 |
| Accession No (Link to NCBI) |
NM_079862.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Kayukawa N, Miki T.
A genetic screen in Drosophila for regulators of human prostate cancer progression.
Biochem Biophys Res Commun (2014) 451(4) 548-55
[
PubMed ID = 25117438
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTTCTGCTGGGTAGCCTGGTG |
| Primer Seq. 3' |
aatctagaggtaccTGCTGCCCATTCTTCTTGGAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTTCTGCTGG GTAGCCTGGT GGCATTCAGC TGGGCAGGAG TCACAACCCA GCCACCGCCA
0061 CTCATACGCA CCCTGAGTGC CGGCGGAGAC ATAGGACCCC AGTTCGACGT GGGGAAGACC
0121 AAGGAACCCG AAGACGCGGA ATTCTGGCAC AACGTGGGCC TGAGGCAGCT GGAGAAGACC
0181 ATTAAGCAGG CGCAGCGCGT GAAGGAGGAC TCCTACCAGA AAAAGGCGCG GAATATCATC
0241 ATCTTCATCG GAGACGGCAT GGGAATATCC ACGATCAGTG CTGGTCGCAT CTACAAGGGG
0301 CAGTACCTGA AGCATGGTTA CGGCGAGGAG GAAACCCTCG TCTTCGACGA TTTCCCAAAC
0361 ACTGGAATGG CCAAAACCTA CAACGTGGAC AAACAAGTGC CGGATTCGGC GGGCACTGCC
0421 ACTGCGATCT TCTCGGGTTC GAAAACCCAT TACGGAGCCA TTGGAATGGN ACGCCACCCG
0481 CTCCAAGAAG AATGGGCCAG CA |
| in silico PCR Fragment |
0001 TTTCTGCTGG GTAGCCTGGT GGCATTCAGC TGGGCAGGAG TCACAACCCA GCCACCGCCA
0061 CTCATACGCA CCCTAAGTGC CGGCGGAGAC ATAGGACCCC AGTTCGACGT GGGGAAGACC
0121 AAGGAACCCG AAGACGCGGA ATTCTGGCAC AACGTGGGCC TGAGGCAGCT GGAGAAGACC
0181 ATTAAGCAGG CGCAGCGCGT GAAGGAGGAC TCCTACCAGA AAAAGGCGCG GAATATCATC
0241 ATCTTCATCG GAGACGGCAT GGGAATATCC ACGATCAGTG CTGGTCGCAT CTACAAGGGG
0301 CAGTACCTGA AGCATGGTTA CGGCGAGGAG GAAACCCTCG TCTTCGACGA TTTCCCAAAC
0361 ACTGGAATGG CCAAAACCTA CAACGTGGAC AAACAAGTGC CGGATTCGGC GGGCACTGCC
0421 ACTGCGATCT TCTCGGGTTC GAAAACCCAT TACGGAGCCA TTGGAATGG- ACGCCACCCG
0481 CTCCAAGAAG AATGGG-CAG CA |
| Assemble Data |
1462R-2.IR_full 1 TTTCTGCTGGGTAGCCTGGTGGCATTCAGCTGGGCAGGAGTCACAACCCAGCCACCGCCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1462R-2.in silico 1 TTTCTGCTGGGTAGCCTGGTGGCATTCAGCTGGGCAGGAGTCACAACCCAGCCACCGCCA 60
1462R-2.IR_full 61 CTCATACGCACCCTGAGTGCCGGCGGAGACATAGGACCCCAGTTCGACGTGGGGAAGACC 120
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
1462R-2.in silico 61 CTCATACGCACCCTAAGTGCCGGCGGAGACATAGGACCCCAGTTCGACGTGGGGAAGACC 120
1462R-2.IR_full 121 AAGGAACCCGAAGACGCGGAATTCTGGCACAACGTGGGCCTGAGGCAGCTGGAGAAGACC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1462R-2.in silico 121 AAGGAACCCGAAGACGCGGAATTCTGGCACAACGTGGGCCTGAGGCAGCTGGAGAAGACC 180
1462R-2.IR_full 181 ATTAAGCAGGCGCAGCGCGTGAAGGAGGACTCCTACCAGAAAAAGGCGCGGAATATCATC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1462R-2.in silico 181 ATTAAGCAGGCGCAGCGCGTGAAGGAGGACTCCTACCAGAAAAAGGCGCGGAATATCATC 240
1462R-2.IR_full 241 ATCTTCATCGGAGACGGCATGGGAATATCCACGATCAGTGCTGGTCGCATCTACAAGGGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1462R-2.in silico 241 ATCTTCATCGGAGACGGCATGGGAATATCCACGATCAGTGCTGGTCGCATCTACAAGGGG 300
1462R-2.IR_full 301 CAGTACCTGAAGCATGGTTACGGCGAGGAGGAAACCCTCGTCTTCGACGATTTCCCAAAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1462R-2.in silico 301 CAGTACCTGAAGCATGGTTACGGCGAGGAGGAAACCCTCGTCTTCGACGATTTCCCAAAC 360
1462R-2.IR_full 361 ACTGGAATGGCCAAAACCTACAACGTGGACAAACAAGTGCCGGATTCGGCGGGCACTGCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1462R-2.in silico 361 ACTGGAATGGCCAAAACCTACAACGTGGACAAACAAGTGCCGGATTCGGCGGGCACTGCC 420
1462R-2.IR_full 421 ACTGCGATCTTCTCGGGTTCGAAAACCCATTACGGAGCCATTGGAATGGNACGCCACCCG 480
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
1462R-2.in silico 421 ACTGCGATCTTCTCGGGTTCGAAAACCCATTACGGAGCCATTGGAATGG-ACGCCACCCG 480
1462R-2.IR_full 481 CTCCAAGAAGAATGGGCCAGCA 502
|||||||||||||||| |||||
1462R-2.in silico 481 CTCCAAGAAGAATGGG-CAGCA 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
3 |
NM_079862.2 |
CG1462-RA, transcript variant A (Aph-4), mRNA |
| 72.82 |
351 |
1 |
1 |
4 |
NM_170534.1 |
CG1462-RB, transcript variant B (Aph-4), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_079994.3 |
CG12630-RA (tio), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_176541.1 |
CG31158-RB, transcript variant B (CG31158), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_170027.2 |
CG31158-RA, transcript variant A (CG31158), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_140264.1 |
CG5897-RA (CG5897), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_078803.1 |
CG4105-RA (Cyp4e3), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079927.2 |
CG5105-RA (Plap), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_142986.2 |
CG5720-RA (CG5720), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_058149.3 |
CG3352-RA (ft), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_057893.3 |
CG5799-RB, transcript variant B (dve), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_057894.3 |
CG5799-RA, transcript variant A (dve), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_141813.2 |
CG6728-RA (ninaG), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140258.2 |
CG7264-RA (CG7264), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001031862.1 |
CG33950-RC, transcript variant C (trol), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001031864.1 |
CG33950-RE, transcript variant E (trol), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001031867.1 |
CG33950-RF, transcript variant F (trol), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001031866.1 |
CG33950-RA, transcript variant A (trol), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001031863.1 |
CG33950-RD, transcript variant D (trol), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001031865.1 |
CG33950-RB, transcript variant B (trol), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_133036.1 |
CG15373-RA (CG15373), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079448.2 |
CG8287-RA (Rab8), mRNA |
| 0 |
0 |
0 |
8 |
16 |
NM_001032052.1 |
CG33715-RE, transcript variant E (Msp-300), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_057863.3 |
CG8418-RA (Ric), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_165793.1 |
CG11883-RA, transcript variant A (CG11883), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_136754.3 |
CG11883-RB, transcript variant B (CG11883), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_206728.1 |
CG9012-RD, transcript variant D (Chc), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_206729.1 |
CG9012-RC, transcript variant C (Chc), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_057694.2 |
CG9012-RA, transcript variant A (Chc), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_167466.1 |
CG9012-RB, transcript variant B (Chc), mRNA |
|
|
|
