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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1403R-1 
 Symbol 2-Sep  Full Name Septin-1 
 CG No CG1403  Old CG No CG1403 
 Synonyms sep1, CG1403, iby, unnamed, tu1, anon-19Fa, Diff6, Sep1 
 Accession No (Link to NCBI) NM_078706.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002 [ PubMed ID = 25977760 ] [ RRC reference ]

Fairchild MJ, Islam F, Tanentzapf G.
Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
PLoS Genet. (2017) 13(9) e1007026 [ PubMed ID = 28957323 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgAAGGGCTTTTCCAGCATCGAG 
 Primer Seq. 3'
aatctagaggtaccGAGTGCAGCTTCTTCATGAAC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 AAGGGCTTTT CCAGCATCGA GACGCCCGGC TATGTGGGCT TGCGCCAACC TGCCCAACCA 
0061 GGTGCATCGA AAGTCCGTGA AAAAGGGATT CGAGTTCACC CTAATGGTGG TGGGCGAGTC 
0121 CGGACTGGGC AAGTCCACGC TGGTCAATAG TCTCTTTCTC ACCGATCTCT ATCCCGAGCG 
0181 CATTATTCCG GACGCCATAG AGAAACAAAA GCAAACCGTA AAGCTGGAGG CATCGACGGT 
0241 GGAGATCGAG GAGCGCGGCG TCNAAGCTAA GATTGACGGT GGTGGACACA CCCGGATTCG 
0301 GCGATGCCAT CGACAACTCC AACAGCTTTG GTGCCATACT CGAGTACATC GATGAGCAAT 
0361 ACNGAGCGCT TCCTGCGCGA CGAGAGCGGC CTGAACAGAC GCAACATTGT GGACAATCGC 
0421 ATTCACTGCT GTTTCTACTT TATATCGCCG TTCGGTCATG GCCTAAAACC CCTGGACGTA 
0481 GAGTTCATGA AGAAGCTGCA CTC 
 in silico PCR Fragment
0001 AAGGGCTTTT CCAGCATCGA GACGCCCGGC TATGTGGGCT T-CGCCAACC TGCCCAACCA 
0061 GGTGCATCGA AAGTCCGTGA AAAAGGGATT CGAGTTCACC CTAATGGTGG TGGGCGAGTC 
0121 CGGACTGGGC AAGTCCACGC TGGTCAATAG TCTCTTTCTC ACCGATCTCT ATCCCGAGCG 
0181 CATTATTCCG GACGCCATAG AGAAACAAAA GCAAACCGTA AAGCTGGAGG CATCGACGGT 
0241 GGAGATCGAG GAGCGCGGCG TC-AAGCTAA GATTGACGGT GGTGGACACA CCCGGATTCG 
0301 GCGATGCCAT CGACAACTCC AACAGCTTTG GTGCCATACT CGAGTACATC GATGAGCAAT 
0361 AC-GAGCGCT TCCTGCGCGA CGAGAGCGGC CTGAACAGAC GCAACATTGT GGACAATCGC 
0421 ATTCACTGCT GTTTCTACTT TATATCGCCG TTCGGTCATG GCCTAAAACC CCTGGACGTA 
0481 GAGTTCATGA AGAAGCTGCA CTC 
 Assemble Data

1403R-1.IR_full       1   AAGGGCTTTTCCAGCATCGAGACGCCCGGCTATGTGGGCTTGCGCCAACCTGCCCAACCA 60
                          ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
1403R-1.in silico     1   AAGGGCTTTTCCAGCATCGAGACGCCCGGCTATGTGGGCTT-CGCCAACCTGCCCAACCA 60


1403R-1.IR_full       61  GGTGCATCGAAAGTCCGTGAAAAAGGGATTCGAGTTCACCCTAATGGTGGTGGGCGAGTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico     61  GGTGCATCGAAAGTCCGTGAAAAAGGGATTCGAGTTCACCCTAATGGTGGTGGGCGAGTC 120


1403R-1.IR_full       121 CGGACTGGGCAAGTCCACGCTGGTCAATAGTCTCTTTCTCACCGATCTCTATCCCGAGCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico     121 CGGACTGGGCAAGTCCACGCTGGTCAATAGTCTCTTTCTCACCGATCTCTATCCCGAGCG 180


1403R-1.IR_full       181 CATTATTCCGGACGCCATAGAGAAACAAAAGCAAACCGTAAAGCTGGAGGCATCGACGGT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico     181 CATTATTCCGGACGCCATAGAGAAACAAAAGCAAACCGTAAAGCTGGAGGCATCGACGGT 240


1403R-1.IR_full       241 GGAGATCGAGGAGCGCGGCGTCNAAGCTAAGATTGACGGTGGTGGACACACCCGGATTCG 300
                          |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
1403R-1.in silico     241 GGAGATCGAGGAGCGCGGCGTC-AAGCTAAGATTGACGGTGGTGGACACACCCGGATTCG 300


1403R-1.IR_full       301 GCGATGCCATCGACAACTCCAACAGCTTTGGTGCCATACTCGAGTACATCGATGAGCAAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico     301 GCGATGCCATCGACAACTCCAACAGCTTTGGTGCCATACTCGAGTACATCGATGAGCAAT 360


1403R-1.IR_full       361 ACNGAGCGCTTCCTGCGCGACGAGAGCGGCCTGAACAGACGCAACATTGTGGACAATCGC 420
                          || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico     361 AC-GAGCGCTTCCTGCGCGACGAGAGCGGCCTGAACAGACGCAACATTGTGGACAATCGC 420


1403R-1.IR_full       421 ATTCACTGCTGTTTCTACTTTATATCGCCGTTCGGTCATGGCCTAAAACCCCTGGACGTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico     421 ATTCACTGCTGTTTCTACTTTATATCGCCGTTCGGTCATGGCCTAAAACCCCTGGACGTA 480


1403R-1.IR_full       481 GAGTTCATGAAGAAGCTGCACTC 503
                          |||||||||||||||||||||||
1403R-1.in silico     481 GAGTTCATGAAGAAGCTGCACTC 503

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_078706.2  CG1403-RA, transcript variant A (Sep1), mRNA 
91.28   440  NM_167747.1  CG1403-RB, transcript variant B (Sep1), mRNA 
4.35   21  16  29  34  NM_165597.1  CG8705-RB, transcript variant B (pnut), mRNA 
4.35   21  16  29  34  NM_057716.3  CG8705-RA, transcript variant A (pnut), mRNA 
2.48   12  22  56  48  NM_206766.1  CG9699-RG, transcript variant G (CG9699), mRNA 
2.48   12  22  56  48  NM_132919.2  CG9699-RC, transcript variant C (CG9699), mRNA 
2.48   12  22  56  48  NM_167531.1  CG9699-RB, transcript variant B (CG9699), mRNA 
2.48   12  22  56  48  NM_167533.1  CG9699-RE, transcript variant E (CG9699), mRNA 
2.48   12  22  56  48  NM_167532.1  CG9699-RD, transcript variant D (CG9699), mRNA 
2.48   12  22  56  48  NM_167534.1  CG9699-RF, transcript variant F (CG9699), mRNA 
2.48   12  22  56  48  NM_167530.1  CG9699-RA, transcript variant A (CG9699), mRNA 
0.2   NM_137707.2  CG30388-RA (Magi), mRNA 
0.2   NM_166197.1  CG5522-RF, transcript variant F (CG5522), mRNA 
0.2   NM_166194.1  CG5522-RB, transcript variant B (CG5522), mRNA 
0.2   NM_137314.2  CG5522-RC, transcript variant C (CG5522), mRNA 
0.2   NM_166195.1  CG5522-RE, transcript variant E (CG5522), mRNA 
0.2   NM_166196.1  CG5522-RD, transcript variant D (CG5522), mRNA 
0.2   NM_166193.1  CG5522-RA, transcript variant A (CG5522), mRNA 
0   NM_079693.2  CG4173-RA (Sep2), mRNA 
0   NM_078673.2  CG7128-RA (Taf8), mRNA 
0   NM_142602.2  CG4424-RA (CG4424), mRNA 
0   NM_001032091.1  CG33650-RA, transcript variant A (DNApol-gamma35), mRNA 
0   NM_001032092.1  CG33649-RA, transcript variant A (CG33649), mRNA 
0   NM_080297.2  CG7413-RA (Rbf), mRNA 
0   NM_170557.1  CG11525-RD, transcript variant D (CycG), mRNA 
0   NM_170556.1  CG11525-RC, transcript variant C (CycG), mRNA 
0   NM_079870.2  CG11525-RA, transcript variant A (CycG), mRNA 
0   NM_170555.1  CG11525-RB, transcript variant B (CycG), mRNA 
0   NM_170558.1  CG11525-RE, transcript variant E (CycG), mRNA 
0   NM_141798.1  CG10703-RA (CG10703), mRNA 

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