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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1403R-1 |
| Symbol |
2-Sep |
Full Name |
Septin-1 |
| CG No |
CG1403 |
Old CG No |
CG1403 |
| Synonyms |
sep1, CG1403, iby, unnamed, tu1, anon-19Fa, Diff6, Sep1 |
| Accession No (Link to NCBI) |
NM_078706.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Sun T, Song Y, Teng D, Chen Y, Dai J, Ma M, Zhang W, Pastor-Pareja JC.<br> Atypical laminin spots and pull-generated microtubule-actin projections mediate Drosophila wing adhesion.<br> Cell Rep (2021) 36(10) 109667
[
PubMed ID = 34496252
]
[
RRC reference
]
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAGGGCTTTTCCAGCATCGAG |
| Primer Seq. 3' |
aatctagaggtaccGAGTGCAGCTTCTTCATGAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAGGGCTTTT CCAGCATCGA GACGCCCGGC TATGTGGGCT TGCGCCAACC TGCCCAACCA
0061 GGTGCATCGA AAGTCCGTGA AAAAGGGATT CGAGTTCACC CTAATGGTGG TGGGCGAGTC
0121 CGGACTGGGC AAGTCCACGC TGGTCAATAG TCTCTTTCTC ACCGATCTCT ATCCCGAGCG
0181 CATTATTCCG GACGCCATAG AGAAACAAAA GCAAACCGTA AAGCTGGAGG CATCGACGGT
0241 GGAGATCGAG GAGCGCGGCG TCNAAGCTAA GATTGACGGT GGTGGACACA CCCGGATTCG
0301 GCGATGCCAT CGACAACTCC AACAGCTTTG GTGCCATACT CGAGTACATC GATGAGCAAT
0361 ACNGAGCGCT TCCTGCGCGA CGAGAGCGGC CTGAACAGAC GCAACATTGT GGACAATCGC
0421 ATTCACTGCT GTTTCTACTT TATATCGCCG TTCGGTCATG GCCTAAAACC CCTGGACGTA
0481 GAGTTCATGA AGAAGCTGCA CTC |
| in silico PCR Fragment |
0001 AAGGGCTTTT CCAGCATCGA GACGCCCGGC TATGTGGGCT T-CGCCAACC TGCCCAACCA
0061 GGTGCATCGA AAGTCCGTGA AAAAGGGATT CGAGTTCACC CTAATGGTGG TGGGCGAGTC
0121 CGGACTGGGC AAGTCCACGC TGGTCAATAG TCTCTTTCTC ACCGATCTCT ATCCCGAGCG
0181 CATTATTCCG GACGCCATAG AGAAACAAAA GCAAACCGTA AAGCTGGAGG CATCGACGGT
0241 GGAGATCGAG GAGCGCGGCG TC-AAGCTAA GATTGACGGT GGTGGACACA CCCGGATTCG
0301 GCGATGCCAT CGACAACTCC AACAGCTTTG GTGCCATACT CGAGTACATC GATGAGCAAT
0361 AC-GAGCGCT TCCTGCGCGA CGAGAGCGGC CTGAACAGAC GCAACATTGT GGACAATCGC
0421 ATTCACTGCT GTTTCTACTT TATATCGCCG TTCGGTCATG GCCTAAAACC CCTGGACGTA
0481 GAGTTCATGA AGAAGCTGCA CTC |
| Assemble Data |
<PRE>
1403R-1.IR_full 1 AAGGGCTTTTCCAGCATCGAGACGCCCGGCTATGTGGGCTT<span class="snp"><tt>G</tt></span>CGCCAACCTGCCCAACCA 60
|||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||
1403R-1.in silico 1 AAGGGCTTTTCCAGCATCGAGACGCCCGGCTATGTGGGCTT<span class="snp"><tt>-</tt></span>CGCCAACCTGCCCAACCA 60
1403R-1.IR_full 61 GGTGCATCGAAAGTCCGTGAAAAAGGGATTCGAGTTCACCCTAATGGTGGTGGGCGAGTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico 61 GGTGCATCGAAAGTCCGTGAAAAAGGGATTCGAGTTCACCCTAATGGTGGTGGGCGAGTC 120
1403R-1.IR_full 121 CGGACTGGGCAAGTCCACGCTGGTCAATAGTCTCTTTCTCACCGATCTCTATCCCGAGCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico 121 CGGACTGGGCAAGTCCACGCTGGTCAATAGTCTCTTTCTCACCGATCTCTATCCCGAGCG 180
1403R-1.IR_full 181 CATTATTCCGGACGCCATAGAGAAACAAAAGCAAACCGTAAAGCTGGAGGCATCGACGGT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico 181 CATTATTCCGGACGCCATAGAGAAACAAAAGCAAACCGTAAAGCTGGAGGCATCGACGGT 240
1403R-1.IR_full 241 GGAGATCGAGGAGCGCGGCGTC<span class="snp"><tt>N</tt></span>AAGCTAAGATTGACGGTGGTGGACACACCCGGATTCG 300
||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||
1403R-1.in silico 241 GGAGATCGAGGAGCGCGGCGTC<span class="snp"><tt>-</tt></span>AAGCTAAGATTGACGGTGGTGGACACACCCGGATTCG 300
1403R-1.IR_full 301 GCGATGCCATCGACAACTCCAACAGCTTTGGTGCCATACTCGAGTACATCGATGAGCAAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico 301 GCGATGCCATCGACAACTCCAACAGCTTTGGTGCCATACTCGAGTACATCGATGAGCAAT 360
1403R-1.IR_full 361 AC<span class="snp"><tt>N</tt></span>GAGCGCTTCCTGCGCGACGAGAGCGGCCTGAACAGACGCAACATTGTGGACAATCGC 420
||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico 361 AC<span class="snp"><tt>-</tt></span>GAGCGCTTCCTGCGCGACGAGAGCGGCCTGAACAGACGCAACATTGTGGACAATCGC 420
1403R-1.IR_full 421 ATTCACTGCTGTTTCTACTTTATATCGCCGTTCGGTCATGGCCTAAAACCCCTGGACGTA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1403R-1.in silico 421 ATTCACTGCTGTTTCTACTTTATATCGCCGTTCGGTCATGGCCTAAAACCCCTGGACGTA 480
1403R-1.IR_full 481 GAGTTCATGAAGAAGCTGCACTC 503
|||||||||||||||||||||||
1403R-1.in silico 481 GAGTTCATGAAGAAGCTGCACTC 503
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_078706.2 |
CG1403-RA, transcript variant A (Sep1), mRNA |
| 91.28 |
440 |
1 |
1 |
2 |
NM_167747.1 |
CG1403-RB, transcript variant B (Sep1), mRNA |
| 4.35 |
21 |
16 |
29 |
34 |
NM_165597.1 |
CG8705-RB, transcript variant B (pnut), mRNA |
| 4.35 |
21 |
16 |
29 |
34 |
NM_057716.3 |
CG8705-RA, transcript variant A (pnut), mRNA |
| 2.48 |
12 |
22 |
56 |
48 |
NM_206766.1 |
CG9699-RG, transcript variant G (CG9699), mRNA |
| 2.48 |
12 |
22 |
56 |
48 |
NM_132919.2 |
CG9699-RC, transcript variant C (CG9699), mRNA |
| 2.48 |
12 |
22 |
56 |
48 |
NM_167531.1 |
CG9699-RB, transcript variant B (CG9699), mRNA |
| 2.48 |
12 |
22 |
56 |
48 |
NM_167533.1 |
CG9699-RE, transcript variant E (CG9699), mRNA |
| 2.48 |
12 |
22 |
56 |
48 |
NM_167532.1 |
CG9699-RD, transcript variant D (CG9699), mRNA |
| 2.48 |
12 |
22 |
56 |
48 |
NM_167534.1 |
CG9699-RF, transcript variant F (CG9699), mRNA |
| 2.48 |
12 |
22 |
56 |
48 |
NM_167530.1 |
CG9699-RA, transcript variant A (CG9699), mRNA |
| 0.2 |
1 |
2 |
2 |
4 |
NM_137707.2 |
CG30388-RA (Magi), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_166197.1 |
CG5522-RF, transcript variant F (CG5522), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_166194.1 |
CG5522-RB, transcript variant B (CG5522), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_137314.2 |
CG5522-RC, transcript variant C (CG5522), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_166195.1 |
CG5522-RE, transcript variant E (CG5522), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_166196.1 |
CG5522-RD, transcript variant D (CG5522), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_166193.1 |
CG5522-RA, transcript variant A (CG5522), mRNA |
| 0 |
0 |
5 |
9 |
9 |
NM_079693.2 |
CG4173-RA (Sep2), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_078673.2 |
CG7128-RA (Taf8), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_142602.2 |
CG4424-RA (CG4424), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_001032091.1 |
CG33650-RA, transcript variant A (DNApol-gamma35), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_001032092.1 |
CG33649-RA, transcript variant A (CG33649), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_080297.2 |
CG7413-RA (Rbf), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_170557.1 |
CG11525-RD, transcript variant D (CycG), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_170556.1 |
CG11525-RC, transcript variant C (CycG), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_079870.2 |
CG11525-RA, transcript variant A (CycG), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_170555.1 |
CG11525-RB, transcript variant B (CycG), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_170558.1 |
CG11525-RE, transcript variant E (CycG), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_141798.1 |
CG10703-RA (CG10703), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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