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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1395R-1 |
| Symbol |
stg |
Full Name |
string |
| CG No |
CG1395 |
Old CG No |
CG1395 |
| Synonyms |
cdc25, str/cdc25, cdc25[string], CDC25[string], stg[cdc25], Cdc25, CG1395, Cdc25[String], String/Cdc25, SY3-4, l(3)01235, clone 2.21, Cdc25[stg], 5473, 1143/02, 1089/08, 1083/13, 0980/06, 0967/05, 0896/05, 0730/13, 0439/22, 0245/03, 0224/06, Cdc25[string], S(rux)3A, EP1213, l(3)s2213, l(3)j3D1, l(3)j1E3, l(3)j1D3, l(3)j10B9, anon-EST:Liang-2.21, stg, Stg |
| Accession No (Link to NCBI) |
NM_079823.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Zhang P, Holowatyj AN, Roy T, Pronovost SM, Marchetti M, Liu H, Ulrich CM, Edgar BA.
An SH3PX1-Dependent Endocytosis-Autophagy Network Restrains Intestinal Stem Cell Proliferation by Counteracting EGFR-ERK Signaling.
Dev Cell (2019) 49(4) 574-589.e5
[
PubMed ID = 31006650
]
[
RRC reference
]
Davis TL, Rebay I.
Antagonistic regulation of the second mitotic wave by Eyes absent-Sine oculis and Combgap coordinates proliferation and specification in the Drosophila retina.
Development (2017) 144(14) 2640-2651
[
PubMed ID = 28619818
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGATCTCGTCGTGCTCGCCGTT |
| Primer Seq. 3' |
aatctagaggtaccCTCTGGGGTCTTTGGTGGTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GATCTCGTCG TGCTCGCCGT TCCCTGGAAC TGATGAGCAT GGACCAGGAG GAGCTGTCGT
0061 TCTACGACGA CGACGTTGTG CCCCAGGATC AGCAGCGATC GGCCAGTCCG GAGCTGATGG
0121 GTCTGCTCTC GCCGGAGGGC TCGCCCCAGC GCNTTCCAGA TCGTCCGCCA GCCGAAAATT
0181 CTGCCAGCTA TGGGAGTATC AAGTGATCAC ACGCCGNGCG CGCAGCTTCC GCATCTTCAA
0241 CAGCCTGTCC TCCACATGCT CCATGGAGTC CTCCATGGAC GATGAGTACA TGGAGNCTCT
0301 TCGAGATGGA GTCGCAGAGC CAACAGACCG CCCTGGGCTT CCCCAGTGGC CTAAACTCGC
0361 TGATCAGCGG CCAGATCAAG GAGCAGCCTG CTGCCAAATC GCCAGCGGGT CTGTCCATGC
0421 GCCGCCCTTC GGTCAGAAGG TGCCTCAGCA TGACGGAGAG CAACACCAAC AGCACCACCA
0481 CCCCACCACC AAAGACCCCA GAG |
| in silico PCR Fragment |
0001 GATCTCGTCG TGCTCGCCGT TCCCTGGAAC TGATGAGCAT GGACCAGGAG GAGCTGTCGT
0061 TCTACGACGA CGACGTTGTG CCCCAGGATC AGCAGCGATC GGCCAGTCCG GAGCTGATGG
0121 GTCTGCTCTC GCCGGAGGGC TCGCCCCAGC GC-TTCCAGA TCGTCCGCCA GCCGAAAATT
0181 CTGCCAGCTA TGGGAGTATC AAGTGATCAC ACGCCG-GCG CGCAGCTTCC GCATCTTCAA
0241 CAGCCTGTCC TCCACATGCT CCATGGAGTC CTCCATGGAC GATGAGTACA TGGAG-CTCT
0301 TCGAGATGGA GTCGCAGAGC CAACAGACCG CCCTGGGCTT CCCCAGTGGC CTAAACTCGC
0361 TGATCAGCGG CCAGATCAAG GAGCAGCCTG CTGCCAAATC GCCAGCGGGT CTGTCCATGC
0421 GCCGCCCTTC GGTCAGAAGG TGCCTCAGCA TGACGGAGAG CAACACCAAC AGCACCACCA
0481 CCCCACCACC AAAGACCCCA GAG |
| Assemble Data |
1395R-1.IR_full 1 GATCTCGTCGTGCTCGCCGTTCCCTGGAACTGATGAGCATGGACCAGGAGGAGCTGTCGT 60
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1395R-1.in silico 1 GATCTCGTCGTGCTCGCCGTTCCCTGGAACTGATGAGCATGGACCAGGAGGAGCTGTCGT 60
1395R-1.IR_full 61 TCTACGACGACGACGTTGTGCCCCAGGATCAGCAGCGATCGGCCAGTCCGGAGCTGATGG 120
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1395R-1.in silico 61 TCTACGACGACGACGTTGTGCCCCAGGATCAGCAGCGATCGGCCAGTCCGGAGCTGATGG 120
1395R-1.IR_full 121 GTCTGCTCTCGCCGGAGGGCTCGCCCCAGCGCNTTCCAGATCGTCCGCCAGCCGAAAATT 180
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1395R-1.in silico 121 GTCTGCTCTCGCCGGAGGGCTCGCCCCAGCGC-TTCCAGATCGTCCGCCAGCCGAAAATT 180
1395R-1.IR_full 181 CTGCCAGCTATGGGAGTATCAAGTGATCACACGCCGNGCGCGCAGCTTCCGCATCTTCAA 240
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1395R-1.in silico 181 CTGCCAGCTATGGGAGTATCAAGTGATCACACGCCG-GCGCGCAGCTTCCGCATCTTCAA 240
1395R-1.IR_full 241 CAGCCTGTCCTCCACATGCTCCATGGAGTCCTCCATGGACGATGAGTACATGGAGNCTCT 300
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1395R-1.in silico 241 CAGCCTGTCCTCCACATGCTCCATGGAGTCCTCCATGGACGATGAGTACATGGAG-CTCT 300
1395R-1.IR_full 301 TCGAGATGGAGTCGCAGAGCCAACAGACCGCCCTGGGCTTCCCCAGTGGCCTAAACTCGC 360
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1395R-1.in silico 301 TCGAGATGGAGTCGCAGAGCCAACAGACCGCCCTGGGCTTCCCCAGTGGCCTAAACTCGC 360
1395R-1.IR_full 361 TGATCAGCGGCCAGATCAAGGAGCAGCCTGCTGCCAAATCGCCAGCGGGTCTGTCCATGC 420
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1395R-1.in silico 361 TGATCAGCGGCCAGATCAAGGAGCAGCCTGCTGCCAAATCGCCAGCGGGTCTGTCCATGC 420
1395R-1.IR_full 421 GCCGCCCTTCGGTCAGAAGGTGCCTCAGCATGACGGAGAGCAACACCAACAGCACCACCA 480
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1395R-1.in silico 421 GCCGCCCTTCGGTCAGAAGGTGCCTCAGCATGACGGAGAGCAACACCAACAGCACCACCA 480
1395R-1.IR_full 481 CCCCACCACCAAAGACCCCAGAG 503
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1395R-1.in silico 481 CCCCACCACCAAAGACCCCAGAG 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
7 |
NM_079823.2 |
CG1395-RA (stg), mRNA |
| 0.2 |
1 |
4 |
5 |
39 |
NM_137711.3 |
CG30389-RA, transcript variant A (CG30389), mRNA |
| 0.2 |
1 |
4 |
5 |
39 |
NM_137712.3 |
CG30389-RC, transcript variant C (CG30389), mRNA |
| 0.2 |
1 |
4 |
5 |
38 |
NM_166428.2 |
CG30389-RB, transcript variant B (CG30389), mRNA |
| 0.2 |
1 |
4 |
4 |
6 |
NM_143085.1 |
CG11848-RA (CG11848), mRNA |
| 0.2 |
1 |
4 |
3 |
13 |
NM_057624.2 |
CG4698-RA (Wnt4), mRNA |
| 0.2 |
1 |
2 |
3 |
17 |
NM_170013.2 |
CG31160-RA (CG31160), mRNA |
| 0 |
0 |
6 |
26 |
49 |
NM_132235.2 |
CG32717-RB, transcript variant B (sdt), mRNA |
| 0 |
0 |
6 |
25 |
42 |
NM_206653.1 |
CG32717-RE, transcript variant E (sdt), mRNA |
| 0 |
0 |
6 |
25 |
42 |
NM_132236.3 |
CG32717-RA, transcript variant A (sdt), mRNA |
| 0 |
0 |
5 |
11 |
34 |
NM_168113.2 |
CG32423-RD, transcript variant D (alan-shepard), mRNA |
| 0 |
0 |
5 |
2 |
3 |
NM_132132.1 |
CG4557-RA (CG4557), mRNA |
| 0 |
0 |
4 |
10 |
37 |
NM_140417.2 |
CG17364-RA, transcript variant A (CG17364), mRNA |
| 0 |
0 |
4 |
9 |
32 |
NM_176328.1 |
CG17364-RB, transcript variant B (CG17364), mRNA |
| 0 |
0 |
4 |
6 |
37 |
NM_078523.2 |
CG2252-RB, transcript variant B (fs(1)h), mRNA |
| 0 |
0 |
4 |
4 |
12 |
NM_133114.2 |
CG32541-RA (CG32541), mRNA |
| 0 |
0 |
4 |
4 |
8 |
NM_132610.2 |
CG4404-RA (CG4404), mRNA |
| 0 |
0 |
4 |
2 |
13 |
NM_080483.2 |
CG6871-RA (Cat), mRNA |
| 0 |
0 |
3 |
10 |
111 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0 |
0 |
3 |
10 |
111 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 0 |
0 |
3 |
10 |
111 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 0 |
0 |
3 |
6 |
4 |
NM_134978.2 |
CG16857-RA (CG16857), mRNA |
| 0 |
0 |
3 |
5 |
17 |
NM_166335.2 |
CG7097-RA, transcript variant A (CG7097), mRNA |
| 0 |
0 |
3 |
4 |
10 |
NM_167777.1 |
CG14619-RC, transcript variant C (CG14619), mRNA |
| 0 |
0 |
3 |
3 |
9 |
NM_132300.2 |
CG12664-RB (ld14), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_169406.2 |
CG31363-RH, transcript variant H (Jupiter), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_169405.2 |
CG31363-RA, transcript variant A (Jupiter), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_169407.2 |
CG31363-RC, transcript variant C (Jupiter), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_169404.2 |
CG31363-RD, transcript variant D (Jupiter), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_169409.3 |
CG31363-RB, transcript variant B (Jupiter), mRNA |
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